Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,878,747 +T 97.6% intergenic (+56/‑170) RCAP_rcc01739 → / → RCAP_rcc01740 hypothetical protein/ABC transporter ATP‑binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,878,7471.→T97.6% 4.3 ‑1.2 ‑1.8169intergenic (+56/‑170)RCAP_rcc01739/RCAP_rcc01740hypothetical protein/ABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  new base (69/94):  ref base (3/1):  total (74/95)
Fisher's exact test strand distribution p-value = 3.16e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.43e-02

Only 100 of 193 total aligned reads displayed.

CACACCTACACGCCGCCGGGCGAGAAACCCGGCAAGGACGCCTGACGCCCGCTTGCCGCGACATCCGACCGCCGAACCCGGCCCCGCCCCGCGGGACC.GGG.TT.TTTGTGAACCGGGCCGGAAAGACGCGCCGCATCGGACCCGCTCGGGATATCTGGCCAAGGCGAAGGGGCAGTCGGGTCCGCTTTCCCCCGGCC  >  NC_014034/1878647‑1878842
                                                                                                      |                                                                                                
cacaCCTACACGCCGCCGGGCGAGAAACCCGGCAAGGACGCCTGACGCCCGCTTGCCGCGACATCCGACCGCCGAACCCGGCCCCGCCCCGCGGGACC.ggg                                                                                                   >  2:3112789/1‑101 (MQ=255)
   cccTACACGCCGCCGGGCGAGAAACCCGGCAAGGACGCCTGACGCCCGCTTGCCGCGACATCCGACCGCCGAACCCGGCCCCGCCCCGCGGGACC.TGG.tt.t                                                                                              <  2:4057065/100‑1 (MQ=255)
     ctccacGCCGCCGGGCGAGAAACCCGGCAAGGACGCCTGACGCCCGCTTGCCGCGACATCCGACCGCCGAACCCGGCCCCGCCCCGCGGGACC.GGG.tt.ttt                                                                                            <  1:2130197/98‑1 (MQ=255)
      tACACGCCGCCGGGCGAGAAACCCGGCAAGGACGCCTGACGCCCGCTTGCCGCGACATCCGACCGCCGAACCCGGCCCCGCCCCGCGGGACC.GGG.tt.tttt                                                                                           >  2:4240972/1‑100 (MQ=255)
      tACACGCCGCCGGGCGAGAAACCCGGCAAGGACGCCTGACGCCCGCTTGCCGCGACATCCGACCGCCGAACCCGGCCCCGCCCCGCGGGACC.GGG.ct.tttt                                                                                           >  2:918712/1‑100 (MQ=255)
       acacGCCGCCGGGCGAGAAACCCGGCAAGGACGCCTGACGCCCGCTTGCCGCGACATCCGACCGCCGAACCCGGCCCCGCCCCGCGGGACC.GGG.tt.ttttg                                                                                          <  2:4520818/101‑3 (MQ=255)
         ccgccgcCGGGCGAGAAACCCGGCAAGGACGCCTGACGCCCGCTTGCCGCGACATCCGACCGCCGAACCCGGCCCCGCCCCGCGGGACC.GGG.tt.ttttgtg                                                                                        <  1:3330230/100‑5 (MQ=255)
         acGCCGCCGGGCGAGAAACCCGGCAAGGACGCCTGACGCCCGCTTGCCGCGACATCCGACCGCCGAACCCGGCCCCGCCCCGCGGGACC.GGG.tt.ttttgtg                                                                                        >  2:2894713/1‑97 (MQ=255)
           gccgccGGGCGAGAAACCCGGCAAGGACGCCTGACGCCCGCTTGCCGCGACATCCGACCGCCGAACCCGGCCCCGCCCCGCGGGACC.GGGTTT.TTTGTGaa                                                                                       >  1:2314754/1‑101 (MQ=255)
           gccgccGGGCGAGAAACCCGGCAAGGACGCCTGACGCCCGCTTGCCGCGACATCCGACCGCCGAACCCGGCCCCGCCCCGCGGGACC.GGGTTT.TTTGTGaa                                                                                       <  1:1767307/101‑1 (MQ=255)
           gccgccGGGCGAGAAACCCGGCAAGGACGCCTGACGCCCGCTTGCCGCGACATCCGACCGCCGAACCCGGCCCCGCCCCGCGGGACC.GGGTTT.TTTGTGaa                                                                                       >  1:1117742/1‑101 (MQ=255)
            cggccgGGCGAGAACCCCGGCAAGGACGCCTGACGCCCGCTTGCCGCGACATCCGACCGCCGAACCCGGCCCCGCCCCGCGGGACC.GGGTTT.TTTGTGAAc                                                                                      <  2:3194955/99‑1 (MQ=255)
            ccgccgGGCGAGAAACCCGGCAAGGACGCCTGACGCCCGCTTGCCGCGACATCCGACCGCCGAACCCGGCCCCGCCCCGCGGGACC.GGGTTT.TTTGTGAAc                                                                                      <  2:1488178/101‑1 (MQ=255)
             cgccgGGCGAGAAACCCGGCAAGGACGCCTGACGCCCGCTTGCCGCGACATCCGACCGCCGAACCCGGCCCCGCCCCGCGGGACC.GGGTTT.TTTGTGAAcc                                                                                     <  2:4158675/101‑1 (MQ=255)
             cgccgGGCGAGAAACCCGGCAAGGACGCCTGACGCCCGCTTGCCGCGACATCCGACCGCCGAACCCGGCCCCGCCCCGCGGGACC.GGGCTT.TTTGTGAAcc                                                                                     >  2:3263962/1‑101 (MQ=255)
              gccgGGCGAGAAGCCCGGCAAGGACGCCTGACGCCCGCTTGCCGCGACATCCGACCGCCGAACCCGGCCCCGCCCCGCGGGACC.GGGTTT.TTTGTGAACCg                                                                                    <  1:455525/101‑1 (MQ=255)
              gccgGGCGAGAAACCCGGCAAGGACGCCTGACGCCCGCTTGCCGCGACATCCGACCGCCGAACCCGGCCCCGCCCCGCGGGACC.GGGTTT.TTTGTGAACCg                                                                                    >  1:2688433/1‑101 (MQ=255)
               ccgGGCGAGAAACCCGGCAAGGACGCCTGACGCCCGCTTGCCGCGACATCCGACCGCCGAACCCGGCCCCGCCCCGCGGGACCGGGG.TT.TTTGTGAACCgg                                                                                   >  1:1027420/1‑101 (MQ=255)
               ccgGGCGAGAAACCCGGCAAGGACGCCTGACGCCCGCTTGCCGCGACATCCGACCGCCGAACCCGGCCCCGCCCCGCGGGACC.GGGTTT.TTTGTGAACCgg                                                                                   <  2:1365751/101‑1 (MQ=255)
                cgGGCGAGAAACCCGGCAAGGACGCCTGACGCCCGCTTGCCGCGACATCCGACCGCCGAACCCGGCCCCGCCCCGCGGGACC.GGGTTT.TTTGTGAACCggg                                                                                  >  1:2087916/1‑101 (MQ=255)
                cgGGCGAGAAACCCGGCAAGGACGCCTGACGCCCGCTTGCCGCGACATCCGACCGCCGAACCCGGCCCCGCCCCGCGGGACC.GGGTTT.TTTGTGAACCggg                                                                                  >  2:2465664/1‑101 (MQ=255)
                 gggCGAGAAACCCGGCAAGGACGCCTGACGCCCGCTTGCCGCGACATCCGACCGCCGAACCCGGCCCCGCCCCGCGGGACC.GGGTTT.TTTGTGAACCGGGc                                                                                 <  2:286215/101‑1 (MQ=255)
                   gCGAGAAACCCGGCAAGGACGCCTGACGCCCGCTTGCCGCGACATCCGACCGCCGAACCCGGCCCCGCCCCGCGGGACCGGGG.TT.TTTGTGAACCGGGCCg                                                                               >  2:4400365/1‑101 (MQ=255)
                       gaAACCCGGCAAGGACGCCTGACGCCCGCTTGCCGCGACATCCGACCGCCGAACCCGGCCCCGCCCCGCGGGACC.GGGTTT.TTTGTGAACCGGGCCGGaaa                                                                           >  1:1282104/1‑101 (MQ=255)
                           cccGGCAAGGACGCCTGACGCCCGCTTGCCGCGACATCCGACCGCCGAACCCGGCCCCGCCCCGCGGGACCGGGG.TT.TTTGTGAACCGGGCCGGAAAGAcg                                                                       >  2:3425440/1‑101 (MQ=255)
                               gCAAGGACGCCTGACGCCCGCTTGCCGCGACATCCGACCGCCGAACCCGGCCCCGCCCCGCGGGACC.GGGTTT.TTTGTGAACCGGGCCGGAAAGACGcgcc                                                                   >  1:733068/1‑101 (MQ=255)
                                cAAGGACGCCTGACGCCCGCTTGCCGCGACATCCGACCGCCGAACCCGGCCCCGCCCCGCGGGACC.GGGTTT.TTTGTGAACCGGGCCGGAAAGACGcgccg                                                                  >  1:2771821/1‑101 (MQ=255)
                                cAAGGACGCCGGACGCCCGCTTGCCGCGACATCCGACCGCCGAACCCGGCCCCGCCCCGCGGGACC.GGGTTT.TTTGTGAACCGGGCCGGAAAGACGcgccg                                                                  <  2:3638936/101‑1 (MQ=255)
                                 aaGGACGCCTGACGCCCGCTTGCCGCGACATCCGACCGCCGAACCCGGCCCCGCCCCGCGGGACC.GGGTTT.TTTGTGAACCGGGCCGGAAAGACGcgccgc                                                                 >  1:1567027/1‑101 (MQ=255)
                                 aaGGACGCCTGACGCCCGCTTGCCGCGACATCCGACCGCCGAACCCGGCCCCGCCCCGCGGGACC.GGGTTT.TTTGTGAACCGGGCCGGAAAGACGcgccgc                                                                 >  1:4197794/1‑101 (MQ=255)
                                 aaGAACGGCTGACGCCCGCGTGGAGCGAGATCCAAGGGCCGAACCCCGTTCCGCCCCGCGGGACCGGGG.TT.TTCGTGCCCAGGGCCGGGAAGACgcgcaaa                                                                 >  2:2060271/1‑98 (MQ=255)
                                  aGGACGCCTGACGCCCGCTTGCCGCGACATCCGACCGCCGAACCCGGCCCCGCCCCGCGGGACC.GGGTTT.TTTGTGAACCGGGCCGGAAAGACGCGCCGCa                                                                >  2:2539805/1‑101 (MQ=255)
                                  aGGACGCCTGACGCCCGCTTGCCGCGACATCCGACCGCCGAACCCGGCCCCGCCCCGCGGGACC.GGGTTT.TTTGTGAACCGGGCCGGAAAGACGCGCCGCa                                                                <  2:211681/101‑1 (MQ=255)
                                   ggACGCCTGACGCCCGCTTGCCGCGACATCCGACCGCCGAACCCGGCCCCGCCCCGCGGGACC.GGGTTT.TTTGTGAACCGGGCCGGAAAGACGCGCCGCAt                                                               >  2:2224514/1‑101 (MQ=255)
                                   ggACGCCTGACGCCCGCTTGCCGCGACATCCGACCGCCGAACCCGGCCCCGCCCCGCGGGACC.GGGTTT.TTTGTGAACCGGGCCGGAAAGACGCGCCGCAt                                                               <  2:2204523/101‑1 (MQ=255)
                                   ggACGCCTGACGCCCGCTTGCCGCGACATCCGACCGCCGAACCCGGCCCCGCCCCGCGGGACC.GGGTTT.TTTGTGAACCGGGCCGGAAAGACGCGCCGCAt                                                               >  1:4409878/1‑101 (MQ=255)
                                    gACGCCTGACGCCCGCTTGCCGCGACATCCGACCGCCGAACCCGGCCCCGCCCCGCGGGACC.GGGTTT.TTTGTGAACCGGGCCGGAAAGACGCGCCGCATc                                                              <  1:4661855/101‑1 (MQ=255)
                                    gACGCCTGACCCCCGCTTGCCGCGACATCCGACCGCCGAACCCGGCCCCGCCCCGCGGGACC.GGGTTT.TTTGTGAACCGGGCCGGAAAGACGCGCCGCATc                                                              <  1:4105243/101‑1 (MQ=255)
                                     aCGCCTGACGCCCGCTTGCCGCGACATCCGACCGCCGAACCCGGCCCCGCCCCGCGGGACC.GGGTTT.TTTGTGAACCGGGCCGGAAAGACGCGCCGCATCg                                                             <  1:2287036/101‑1 (MQ=255)
                                       gCCTGCCGCCCGCTTGCCGCGACATCCGACCGCCGAACCCGGCCCCGCCCCGCGGGACC.GGGTTT.TTTGTGAACCGGGCCGGAAAGACGCGCCGCATCGGa                                                           <  1:1369085/101‑1 (MQ=255)
                                       gCCTGACGCCCGCTTGCCGCGACATCCGACCGCCGAACCCGGCCCCGCCCCGCGGGACC.GGGTTT.TTTGTGAACCGGGCCGGAAAGACGCGCCGCATCggc                                                           >  2:2807072/1‑100 (MQ=255)
                                        ccTGGCCCCCGCTTGCCGCGACATCCGACCGCCGAACCCGGCCCCGCCCCGCGGGACC.GGGTTT.TTTGTGAACCGGGCCGGAAAGACGCGCCGCATCGGAc                                                          <  1:3786215/101‑1 (MQ=255)
                                        ccTGACGCCCGCTTGCCGCGACATCCGACCGCCGAACCCGGCCCCGCCCCGCGGGACC.GGGTTT.TTTGTGAACCGGGCCGGAAAGACGCGCCGCATCGGAc                                                          <  2:1211893/101‑1 (MQ=255)
                                         cTGACGGCCGCTTGCCGCGACATCCGACCGCCGAACCCGGCCCCGCCCCGCGGGACC.GGGTTT.TTTGTGAACCGGGCCGGAAAGACGCGCCGCATCGGAcc                                                         <  1:777440/101‑1 (MQ=255)
                                         cTGACGCCCGCTTGCCGCGACATCCGACCGCCGAACCCGGCCCCGCCCCGCGGGACC.GGGTTT.TTTGTGAACCGGGCCGGAAAGACGCGCCGCATCGGAcc                                                         <  1:3113033/101‑1 (MQ=255)
                                           gACGCCCGCTTGCCGCGACATCCGACCGCCGAACCCGGCCCCGCCCCGCGGGACC.GGGTTT.TTTGTGAACCGGGCCGGAAAGACGCGCCGCATCGGACCCg                                                       >  1:218175/1‑101 (MQ=255)
                                           gACGCCCGCTTGCCGCGACATCCGACCGCCGAACCCGGCCCCGCCCCGCGGGACC.GGGTTT.TTTGTGAACCGGGCCGGAAAGACGCGCCGCATCGGACCCg                                                       <  2:2766544/101‑1 (MQ=255)
                                              gCCCGCTTGCCGCGACATCCGACCGCCGAACCCGGCCCCGCCCCGCGGGACCGGGG.TT.TTTGTGAACCGGGCCGGAAAGACGCGCCGCATCGGACCCGCTc                                                    >  2:3161672/1‑101 (MQ=255)
                                              gCCCGCTTGCCGCGACATCCGACCGCCGAACCCGGCCCCGCCCCGCGGGACC.GGGTTT.TTTGTGAACCGGGCCGGAAAGACGCGCCGCATCGGACCCGccc                                                    >  2:390908/1‑99 (MQ=255)
                                              gCCCGCTTGCCGCGACATCCGACCGCCGAACCCGGCCCCGCCCCGCGGGACC.GGGTTT.TTTGTGAACCGGGCCGGAAAGACGCGCCGCATCGGACCCGCTc                                                    <  1:225451/101‑1 (MQ=255)
                                                 cGCTTGCCGCGACATCCGACCGCCGAACCCGGCCCCGCCCCGCGGGACC.GGGTTT.TTTGTGAACCGGGCCGGAAAGACGCGCCGCATCGGACCCGCTCggg                                                 <  2:4040998/101‑1 (MQ=255)
                                                 cGCTTGCCGCGACATCCGACCGCCGAACCCGGCCCCGCCCCGCGGGACC.GGGTTT.TTTGTGAACCGGGCCGGAAAGACGCGCCGCATCGGACCCGCTCggg                                                 >  1:3903188/1‑101 (MQ=255)
                                                   cTTGCCGCGACATCCGACCGCCGAACCCGGCCCCGCCCCGCGGGACC.GGGTTT.TTTGTGAACCGGGCCGGAAAGACGCGCCGCATCGGACCCGCTCGGGat                                               >  2:1721855/1‑101 (MQ=255)
                                                     tGCCGCGACATCCGACCGCCGAACCCGGCCCCGCCCCGCGGGACC.GGGTTT.TTTGTGAACCGGGCCGGAAAGACGCGCCGCATCGGACCCGCTCGGGatat                                             >  2:3281233/1‑101 (MQ=255)
                                                      gCCGCGACATCCGACCGCCGAACCCGGCCCCGCCCCGCGGGACC.GGGTTT.TTTGTGAACCGGGCCGGAAAGACGCGCCGCATCGGACCCGCTCGGGATATc                                            >  2:4632244/1‑101 (MQ=255)
                                                         gcgACATCCGACCGCCGAACCCGGCCCCGCCCCGCGGGACC.GGGTTT.TTTGTGAACCGGGCCGGAAAGACGCGCCGCATCGGACCCGCTCGGGATATCTgg                                         >  1:2411535/1‑101 (MQ=255)
                                                         gcgACATCCGACCGCCGAACCCGGCCCCGCCCCGCGGGACC.GGGTTT.TTTGTGAACCGGGCCGGAAAGACGCGCCGCATCGGACCCGCTCGGGATATCTgg                                         <  2:57561/101‑1 (MQ=255)
                                                           gACATCCGACCGCCGAACCCGGCCCCGCCCCGCGGGACC.GGGTTT.TTTGTGAACCGGGCCGGAAAGACGCGCCGCATCGGACCCGCTCGGGATATCTGGcc                                       <  1:4508017/101‑1 (MQ=255)
                                                           gACATCCGACCGACGAACCCGGCCCCGCCCCGCGGGACC.GGGTTT.TTTGTGAACCGGGCCGGAAAGACGCGCCGCATCGGACCCGCTCGGGGTATCtaccc                                       >  2:1261407/1‑97 (MQ=255)
                                                            aCATCCGACCGCCGAACCCGGCCCCGCCCCGCGGGACC.GGGTTT.TTTGTGAACCGGGCCGGAAAGACGCGCCGCATCGGACCCGCTCGGGATATCTGGCCa                                      <  2:827424/101‑1 (MQ=255)
                                                             cATCCGACCGCCGAACCCGGCCCCGCCCCGCGGGACC.GGGTTT.TTTGTGAACCGGGCCGGAAAGACGCGCCGCATCGGACCCGCTCGGGATATCTGGCCaa                                     >  1:347001/1‑101 (MQ=255)
                                                              tgtgctcttccgATCTCGGCCCCGCCCCGCGGGACC.GGGTTT.TTTGTGAACCGGGCCGGAAAGACGCGCCGCATCGGACCCGCTCGGGATATCTGGCCAAg                                    <  1:3040587/92‑1 (MQ=255)
                                                               tCCGCCCGCCGAACCCGGCCCCGCCCCGCGGGACC.GGGTTT.TTTGTGAACCGGGCCGGAAAGACGCGCCGCATCGGACCCGCTCGGGATATCTGGCCAAgg                                   <  1:918792/101‑1 (MQ=255)
                                                                  gACCGCCGAACCCGGCCCCGCCCCGCGGGACC.GGGTTT.TTTGTGAACCGGGCCGGAAAGACGCGCCGCATCGGACCCGCTCGGGATATCTGGCCAAGGCGa                                <  2:3722315/101‑1 (MQ=255)
                                                                   cccgccgAACCCGGCCCCGCCCCGCGGGACC.GGGTTT.TTTGTGAACCGGGCCGGAAAGACGCGCCGCATCGGACCCGCTCGGGATATCTGGCCAAGGCGaa                               <  1:4617025/100‑1 (MQ=255)
                                                                   aCCGCCGAACCCGGCCCCGCCCCGCGGGACC.GGGTTT.TTTGTGAACCGGGCCGGAAAGACGCGCCGCATCGGACCCGCTCGGGATATCTGGCCAAGGCGaa                               >  2:628013/1‑101 (MQ=255)
                                                                   aCCGCCGAACCCGGCCCCGCCCCGCGGGACC.GGGTTT.TTTGTGAACCGGGCCGGAAAGACGCGCCGCATCGGACCCGCTCGGGATATCTGGCCAAGGCGaa                               <  2:4502241/101‑1 (MQ=255)
                                                                     cgccgACCCCGGCCCCGCCCCGCGGGACC.GGGTTT.TTTGTGAACCGGGCCGGAAAGACGCGCCGCATCGGACCCGCTCGGGATATCTGGCCAAGGCGAAgg                             <  2:3496497/101‑1 (MQ=255)
                                                                      gccgAACCCGGCCCCGCCCCGCGGGACC.GGGTTT.TTTGTGAACCGGGCCGGAAAGACGCGCCGCATCGGACCCGCTCGGGATATCTGGCCAAGGCGAAggg                            <  1:3430248/101‑1 (MQ=255)
                                                                      gccgAACCCGGCCCCGCCCCGCGGGACC.GGGTTT.TTTGTGAACCGGGCCGGAAAGACGCGCCGCATCGGACCCGCTCGGGATATCTGGCCAAGGCGAAggg                            <  1:2898578/101‑1 (MQ=255)
                                                                      cccgAACCCGGCCCCGCCCCGCGGGACC.GGGTTT.TTTGTGAACCGGGCCGGAAAGACGCGCCGCATCGGACCCGCTCGGGATATCTGGCCAAGGCGAAggg                            <  2:3186994/100‑1 (MQ=255)
                                                                         gAACCCGGCCCCGCCCCGCGGGACC.GGGTTT.TTTGTGAACCGGGCCGGAAAGACGCGCCGCATCGGACCCGCTCGGGATATCTGGCCAAGGCGAAGGGGCa                         >  2:268299/1‑101 (MQ=255)
                                                                         gAACCCGGCCCCGCCCCGCGGGACC.GGGTTT.TTTGTGAACCGGGCCGGAAAGACGCGCCGCATCGGACCCGCTCGGGATATCTGGCCAAGGCGAAGGGGCa                         >  2:2009158/1‑101 (MQ=255)
                                                                         gAACCCGGCCCCGCCCCGCGGGACC.GGGTTT.TTTGTGAACCGGGCCGGAAAGACGCGCCGCATCGGACCCGCTCGGGATATCTGGCCAAGGCGAAGGGGCa                         >  2:3362866/1‑101 (MQ=255)
                                                                          aaCCCGGCCCCGCCCCGCGGGACC.GGGTTT.TTTGTGAACCGGGCCGGAAAGACGCGCCGCATCGGACCCGCTCGGGATATCTGGCCAAGGCGAAGGGGCAg                        >  2:2780526/1‑101 (MQ=255)
                                                                            cccGGCCCCGCCCCGCGGGACC.GGGTTT.TTTGTGAACCGGGCCGGAAAGACGCGCCGCATCGGACCCGCTCGGGATATCTGGCCAAGGCGAAGGGGCAGTc                      <  1:2046108/101‑1 (MQ=255)
                                                                             ccGGCGCCGCCCCGCGGGGCC.GGT.TTATTTGTGAACCGGGCCGGAAAGACGCTCTGCATCGGACCTGCTCGGGATATCTGGCCAAGGCGAAGGGGCAGTCg                     <  2:1532144/101‑1 (MQ=255)
                                                                             ccGGCCCCGCCCCGCGGGACC.GGGTTT.TTTGTGAACCGGGCCGGAAAGACGCGCCGCATCGGACCCGCTCGGGATATCTGGCCAAGGCGAAGGGGCAGTCg                     <  2:4358680/101‑1 (MQ=255)
                                                                              cGGCCCCGCCCCGCGGGACC.GGGTTT.TTTGTGAACCGGGCCGGAAAGACGCGCCGCATCGGACCCGCTCGGGATATCTGGCCAAGGCGAAGGGGCAGTCgg                    >  2:2300206/1‑101 (MQ=255)
                                                                               ggacccgccccgcGGGACC.GGGTTT.TTTGTGAACCGGGCCGGAAAGACGCGCCGCATCGGACCCGCTCGGGATATCTGGCCAAGGCGAAGGGGCAGTCggg                   >  1:3448528/4‑101 (MQ=255)
                                                                               ggCCCCGCCCCGCGGGACC.GGGTTT.TTTGTGAACCGGGCCGGAAAGACGCGCCGCATCGGACCCGCTCGGGATATCTGGCCAAGGCGAAGGGGCAGTCggg                   >  1:2193810/1‑101 (MQ=255)
                                                                                gccccgccccGCGGGACC.GGGTTT.TTTGTGAACCGGGCCGGAAAGACGCGCCGCATCGGACCCGCTCGGGATATCTGGCCAAGGCGAAGGGGCAGTCgggg                  >  1:1977285/1‑100 (MQ=255)
                                                                                  cccgccccgcGGGACC.GGGTTT.TTTGTGAACCGGGCCGGAAAGACGCGCCGCATCGGACCCGCTCGGGATATCTGGCCAAGGCGAAGGGGCAGTCGGGTcc                <  2:3760119/101‑1 (MQ=255)
                                                                                  cccgccccgcGGGACC.GGGTTT.TTTGTGAACCGGGCCGGAAAGACGCGCCGCATCGGACCAGCTCGGGATATCTGGCCAAGGCGAAGGGGCAGTCGGGGcc                >  2:1045512/1‑101 (MQ=255)
                                                                                   ccgccccgcGGGACC.GGGTTT.TTTGTGAACCGGGCCGGAAAGACGCGCCGCATCGGACCCGCTCGGGATATCTGGCCAAGGCGAAGGGGCAGTCGGGTCCg               >  1:3619913/1‑101 (MQ=255)
                                                                                    cgccccgcGGGACC.GGGTTT.TTTGTGAACCGGGCCGGAAAGACGCGCCGCATCGGACCCGCTCGGGATATCTGGCCAAGGCGAAGGGGCAGTCGGGTCCGc              >  1:1804106/1‑101 (MQ=255)
                                                                                      ccccgcGGGACC.GGGTTT.TTTGTGACCCGGGCCGGAAAGACGCGCCGCATCGGACCCGCTCGGGATATCTGGCCAAGGCGAAGGGGCAGTCGGGTCCGCtt            <  2:1395427/101‑1 (MQ=255)
                                                                                      ccccgcGGGACC.GGGTTT.TTTGTGAACCGGGCCGGAAAGACGCGCCGCATCGGACCCGCTCGGGATATCTGGCCAAGGCGAAGGGGCAGTCGGGTCCGCtt            <  2:1803987/101‑1 (MQ=255)
                                                                                      ccccgcGGGACC.GGGTTT.TTTGTGAACCGGGCCGGAAAGACGCGCCGCATCGGACCCGCTCGGGATATCTGGCCAAGGCGAAGGGGCAGTCGGGTCCGCtt            <  2:4077/101‑1 (MQ=255)
                                                                                       cccgcGGGACC.GGGTTT.TTTGTGAACCGGGCCGGAAAGACGCGCCGCATCGGACCCGCTCGGGATATCTGGCCAAGGCGAAGGGGCAGTCGGGTCCGCttt           <  1:5604/101‑1 (MQ=255)
                                                                                       cccgcGGGACC.GGGTTT.TTTGTGAACCGGGCCGGAAAGACGCGCCGCATCGGACCCGCTCGGGATATCTGGCCAAGGCGAAGGGGCAGTCGGGTCCGCttt           >  1:345984/1‑101 (MQ=255)
                                                                                        ccgcGGGACC.GGGTTT.TTTGTGAACCGGGCCGGAAAGACGCGCCGCATCGGACCCGCTCGGGATATCTGGCCAAGGCGAAGGGGCAGTCGGGTCCGCTTTc          <  1:2539958/101‑1 (MQ=255)
                                                                                        ccgcGGGACC.GGGTTT.TTTGTGAACCGGGCCGGAAAGACGCGCCGCATCGGACCCGCTCGGGATATCTGGCCAAGGCGAAGGGGCAGTCGGGTCCGCTTTc          <  2:3122573/101‑1 (MQ=255)
                                                                                          gcgGGACC.GGGTTT.TTTGTGAACCGGGCCGGAAAGACGCGCCGCATCGGACCCGCTCGGGATATCTGGCCAAGGCGAAGGGGCAGTCGGGTCCGCTTTccc        >  2:53302/1‑101 (MQ=255)
                                                                                            gggACC.GGGTTT.TTTGTGAACCGGGCCGGAAAGACGCGCCGCATCGGACCCGCTCGGGATATCTGGCCAAGGCGAAGGGGCAGTCGGGTCCGCTTTccccc      >  1:1468987/1‑101 (MQ=255)
                                                                                            gggACC.GGGTTT.TTTGTGAACCGGGCCGGAAAGACGCGCCGCATCGGACCCGCTCGGGATATCTGGCCAACGCGAAGGGGCAGTCGGGTCCGCTTTccccc      <  1:4152649/101‑1 (MQ=255)
                                                                                             ggACC.GGGTTT.TTTGTGAACCGGGCCGGAAAGGCGCGCCGCATCGGACCCGCTCGGGATATCTGGCCAAGGCGAAGGGGCAGTCGGGTCCGCTTTCCCCCg     <  2:291595/101‑1 (MQ=255)
                                                                                             ggACC.GGGTTT.TTTGTGAACCGGGCCGGAAAGACGCGCCGCATCGGACCCGCTCGGGATATCTGGCCAAGGCGAAGGGGCAGTCGGGTCCGCTTTCCCCCg     >  1:1507334/1‑101 (MQ=255)
                                                                                               aCC.GGGTTT.TTTGTGAACCGGGCCGGAAAGACGCGCCGCATCGGACCCGCTCGGGATATCTGGCCAAGGCGAAGGGGCAGTCGGGTCCGCTTTCCCCCGGc   >  1:1043148/1‑101 (MQ=255)
                                                                                               aCC.GGGTTT.TTTGTGAACCGGGCCGGAAAGACGCGCCGCATCGGACCCGCTCGGGATATCTGGCCAAGGCGAAGGGGCAGTCGGGTCCGCTTTCCCCCGGc   <  2:283992/101‑1 (MQ=255)
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CACACCTACACGCCGCCGGGCGAGAAACCCGGCAAGGACGCCTGACGCCCGCTTGCCGCGACATCCGACCGCCGAACCCGGCCCCGCCCCGCGGGACC.GGG.TT.TTTGTGAACCGGGCCGGAAAGACGCGCCGCATCGGACCCGCTCGGGATATCTGGCCAAGGCGAAGGGGCAGTCGGGTCCGCTTTCCCCCGGCC  >  NC_014034/1878647‑1878842

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG