Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,925,099 +C 100% intergenic (+39/+6) RCAP_rcc01778 → / ← RCAP_rcc01779 hypothetical protein/MazG family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,925,0991.→C100.0% 14.4 181intergenic (+39/+6)RCAP_rcc01778/RCAP_rcc01779hypothetical protein/MazG family protein
Reads supporting (aligned to +/- strand):  new base (106/67):  ref base (0/7):  total (107/74)
Fisher's exact test strand distribution p-value = 1.67e-03
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.02e-01

Only 100 of 212 total aligned reads displayed.

GCCTCTTCGCAGGGTTTCTGATGCTGGTCGCGCTGAACATGCTGCGCAAGGCGCTGTTCTGATTTTATCTTGCGGCAAATATCCC.GGGGTGCGGGGCAGA.G.CCCGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCATCTCGGCCAGATCGCTGTCGGCGGGCCGCTTGCCGGCCTTCGCCAG  >  NC_014034/1924999‑1925191
                                                                                                       |                                                                                            
  ctctTCGCAGGGTTTCTGATGCTGGTCGCGCTGAACATGCTGCGCAAGGCGCTGTTCTGATTTTATCTTGCGGCAAATATCCC.GGGGTGCGGGGCAGA.G.cc                                                                                            >  1:1366264/1‑101 (MQ=255)
  ctctTCGCAGGGTTTCTGATGCTGGTCGCGCTGAACATGCTGCGCAAGGCGCTGTTCTGATTTTATCTTGCGGCAAATATCCC.GGGGTGCGGGGCAGA.G.cc                                                                                            >  2:351530/1‑101 (MQ=255)
   tctTCGCAGGGTTTCTGATGCTGGTCGCGCTGAACATGCTGCGCAAGGCGCTGTTCTGATTTTATCTTGCGGCAAATATCCC.GGGGTGCGGGGCAGA.G.ccc                                                                                           >  2:4139849/1‑101 (MQ=255)
   tctTCGCAGGGTTTCTGATGCTGGTCGCGCTGAACATGCTGCGCAAGGCGCTGTTCTGATTTTATCTTGCGGCAAATATCCC.GGGGTGCGGGGCAGA.G.ccc                                                                                           >  2:3715033/1‑101 (MQ=255)
    ctTCGCAGGGTTTCTGATGCTGGTCGCGCTGAACATGCTGCGCAAGGCGCTGTTCTGATTTTATCTTGCGGCAAATATCCC.GGGGTGCGGGGCAGA.G.cccc                                                                                          >  2:2723227/1‑100 (MQ=255)
    ctTCGCAGGGTTTCTGATGCTGGTCGCGCTGAACATGCTGCGCAAGGCGCTGTTCTGATTTTATCTTGCGGCAAATATCCC.GGGGTGCGGGGCAGA.G.cccc                                                                                          >  1:2464645/1‑100 (MQ=255)
    cgtCGCAGGGTTTCTGATGCTGGTCGCGCTGAACATGCTGCGCAAGGCGCTGTTCTGATTTTATCTTGCGGCAAATATCCC.GGGGTGCGGGGCAGA.G.cccc                                                                                          <  2:4625648/99‑2 (MQ=255)
     ttCGCAGGGTTTCTGATGCTGGTCGCGCTGAACATGCTGCGCAAGGCGCTGTTCTGATTTTATCTTGCGGCAAATATCCC.GGGGTGCGGGGCAGA.G.ccccg                                                                                         <  2:2834063/101‑3 (MQ=255)
     ttCGCAGGGTGTCTGATGCTGGGCGCGCTGAACATGCTGCGCAAGGCGCTGTTCTGATTTTAGCTTGCGGCAAATATCCC.GGGGTGCGGGGCAGA.G.ccccg                                                                                         <  2:2233554/101‑3 (MQ=255)
      tCGCAGGGTTTCTGATGCTGGTCGCGCTGAACATGCTGCGCAAGGCGCTGTTCTGATTTTATCTTGCGGCAAATATCCC.GGGGTGCGGGGCAGA.G.ccccgc                                                                                        >  2:3624820/1‑98 (MQ=255)
       cGCAGGGTTTCTGATGCTGGTCGCGCTGAACATGCTGCGCAAGGCGCTGTTCTGATTTTATCTTGCGGCAAATATCCC.GGGGTGCGGGGCAGA.G.ccccgcc                                                                                       >  2:2773038/1‑97 (MQ=255)
       cGCAGGGTTTCTGATGCTGGTCGCGCTGAACATGCTGCGCAAGGCGCTGTTCTGATTTTATCTTGCGGCAAATATCCC.GGGGTGCGGGGCAGA.G.ccccgcc                                                                                       <  1:1617618/101‑5 (MQ=255)
        gCAGGGTTTCTGATGCTGGTCGCGCTGAACATGCTGCGCAAGGCGCTGTTCTGATTTTATCTTGCGGCAAATATCCC.GGGGTGCGGGGCAGA.G.ccccgcct                                                                                      <  2:1375060/101‑6 (MQ=255)
         cAGGGTTTCTGATGCTGGTCGCGCTGAACATGCTGCGCAAGGCGCTGTTCTGATTTTATCTTGCGGCAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCCTa                                                                                      <  1:3283596/101‑1 (MQ=255)
         cAGGGTTTCTGATGCTGGTCGCGCTGAACATGCTGCGCAAGGCGCTGTTCTGATTTTATCTTGCGGCAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCCTa                                                                                      >  1:2782420/1‑101 (MQ=255)
          aGGTTTTCTGATGCTGGTCGCGCTGAACATGCTGCGCAAGGCGCTGTTCTGATTTTATCTTGCGGCAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCCTAc                                                                                     <  2:3406344/101‑1 (MQ=255)
          aGGGTTTCTGATGCTGGTCGCGCTGAACATGCTGCGCAAGGCGCTGTTCTGATTTTATCTGGCGGCAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCCTAc                                                                                     <  2:3337759/101‑1 (MQ=255)
           gggTTTCTGATGCTGGTCGCGCTGAACATGCTGCGCAAGGCGCTGTTCTGATTTTATCTTGCGGCAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCCTACa                                                                                    <  1:1894586/101‑1 (MQ=255)
           gggTTTCTGATGCTGGTCGCGCTGAACATGCTGCGCAAGGCGCTGTTCTGATTTTATCTTGCGGCAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCCTACa                                                                                    <  2:446634/101‑1 (MQ=255)
            ggTTTCTGATGCTGGTCGCGCTGAACATGCTGCGCAAGGCGCTGTTCTGATTTTATCTTGCGGCAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCCTACAg                                                                                   <  2:3696830/101‑1 (MQ=255)
            ggTTTCTGATGCTGGTCGCGCTGAACATGCTGCGCAAGGCGCTGTTCTGATTTTATCTTGCGGCAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCCTACAg                                                                                   <  2:3109458/101‑1 (MQ=255)
              tttCTGATGCTGGTCGCGCTGAACATGCTGCGCAAGGCGCTGTTCTGATTTTATCTTGCGGCAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCCTACAGcc                                                                                 <  1:3705331/101‑1 (MQ=255)
              tttCTGATGCTGGTCGCGCTGAACATGCTGCGCAAGGCGCTGTTCTGATTTTATCTTGCGGCAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCCTACAGcc                                                                                 <  1:449221/101‑1 (MQ=255)
                tCTGATGCTGGTCGCGCTGAACATGCTGCGCAAGGCGCTGTTCTGTTTTTATCTTGCGGAAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCCTACAGcccc                                                                               <  1:3635671/101‑1 (MQ=37)
                tCTGATGCTGGTCGCGCTGAACATGCTGCGCAAGGCGCTGTTCTGATTTTATCTTGCGGCAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCCTACAGcccc                                                                               >  1:427800/1‑101 (MQ=255)
                  tGATGCTGGTCGCGCTGAACATGCTGCGCAAGGCGCTGTTCTGATTTTATCTTGCGGCAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCCTACAGCCCCtt                                                                             >  2:4525051/1‑101 (MQ=255)
                    aTGCTGGTCGCGCTGAACATGCTGCGCAAGGCGCTGTTCTGATTTTATCTTGCGGCAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCCTACAGCCCCTTgc                                                                           >  1:507565/1‑101 (MQ=255)
                       cTGGTCGCGCTGAACATGCTGCGCAAGGCGCTGTTCTGATTTTATCTTGCGGCAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCCTACAGCCCCTTGCGCt                                                                        >  1:728019/1‑101 (MQ=255)
                        tgtccgcgCTAAAAATGCGGCGCAAGCCGCAGTTCTGTTTTTGTCTTGCGACAAATACCCG.GGGGTGCGGGCCAGA.GCCCCGCCTACAGCCCCTTGCGCtt                                                                       <  2:1832426/97‑1 (MQ=255)
                        tGGTCGCGCTGAACATGCTGCGCAAGGCGCTGTTCTGATTTTATCTTGCGGCAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCCTACAGCCCCTTGCGCtt                                                                       >  2:1329290/1‑101 (MQ=255)
                         ggTCGAGCTGAAAATGCTGCTCAAGGTCCTGTTCTGATTTTATTTTGCGGCAATTTACCC.GTGGTGCGGGGCAGA.GCCCCGCCTACAGCCCCTTGCGCTTg                                                                      <  2:716259/101‑1 (MQ=255)
                            cgcgCGGAACATGCTGCGCAAGGCGCTGTTCTGATTTTATCTTGCGGCAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCCTACAGCCCCTTGCGCTTGGcc                                                                   <  1:579745/101‑1 (MQ=255)
                             gcgcTGAACATGCTGCGCAAGGCGCTGTTCTGATTTTATCTTGCGGCAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCCTACAGCCCCTTGCGCTTGGCCg                                                                  >  2:1504685/1‑101 (MQ=255)
                                cTGAACATGCTGCGCAAGGCGCTGTTCTGATTTTATCTTGCGGCAAATATCCC.GGGGGGCGGGGCAGA.GCCCCGCCTACAGCCCCTTGCGCTTGGCCGCAt                                                               <  1:2054951/101‑1 (MQ=255)
                                 tGAACATGCTGCGCAAGGCGCTGTTCTGATTTTATCTTGCGGCAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCCTACAGCCCCTTGCGCTTGGCCGCATc                                                              >  2:376796/1‑101 (MQ=255)
                                  gacgaTGCTGCGCCAGGCGCTGTTCTTATTTTATCTTGCGGCAAATATCGC.GGGGCGGGGGCAAGA.GCCCCGCCTACAGCCCCTTGCGCTTGGCCGCATcc                                                             <  2:2026776/97‑1 (MQ=255)
                                  gAACATGCTGCGCAAGGCGCTGTTCTGATTTTATCTTGCGGAAAATACCCC.GGGGTGCGGGGCAGA.GCCCCGCCTACAGGCCCTTGCGCTTGGCCGCATcc                                                             <  1:2648498/101‑1 (MQ=37)
                                     ctggctgcGCAAGGCGCTGTTCTGATTTTATCTTGCGGCAAATACCCG.GGGGTGCGGGGCAGA.GCCCCGCCTACAGCCCCTTGCGCTTGGCCGCATCCCaa                                                          <  2:4360397/98‑1 (MQ=38)
                                     cATGCTGCGCAAGGCGCTGTTCTGATTTTATCTTGCGGCAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCCTACAGCCCCTTGCGCTTGGCCGCATCCCaa                                                          >  2:1897280/1‑101 (MQ=255)
                                      aTGCTGCGCAAGGCGCTGTTCTGATTTTATCTTGCGGCAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCCTACAGCCCCTTGCGCTTGGCCGCATCCCaaa                                                         >  2:1645535/1‑101 (MQ=255)
                                      aTGCTGCGCAAGGCGCTGTTCTGATTTTATCTTGCGGCAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCCTACAGCCCCTTGCGCTTGGCCGCATCCCaaa                                                         <  1:2233049/101‑1 (MQ=255)
                                        gctgcGCAAGGCGCTGTTCTGATTTTATCTTGCGGCAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAgc                                                       <  2:1269508/101‑1 (MQ=255)
                                         ctgcGCAAGGCGCTGTTCTGATTTTATCTTGCGGCAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAgcg                                                      >  1:4155515/1‑101 (MQ=255)
                                             gcAAGGCGCTGTTCTGATTTTATCTTGCGGCAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTc                                                  >  1:3213439/1‑101 (MQ=255)
                                                aGGCGCTGTTCTGATTTTATCTTGCGGCAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCAt                                               >  1:1028615/1‑101 (MQ=255)
                                                aGGCGCTGTTCTGATTTTATCTTGCGGCAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCAt                                               <  2:2982589/101‑1 (MQ=255)
                                                  gcgtTGTTCTGATTTTATCTTGCGGCAAATATCCC.GGGGTAGGGGGCAGA.GCCCCGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCAtct                                             <  1:4629510/101‑1 (MQ=255)
                                                    gcTGTTCTGATTTTATCTTGCGGCAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCATCTCg                                           >  2:1385372/1‑101 (MQ=255)
                                                     cTGTTCTGATTTTATCTTGCGGCAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCATCTCgg                                          >  2:543226/1‑101 (MQ=255)
                                                     cTGTTCTGATTTTATCTTGCGGCAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCATCTCgg                                          >  2:4346411/1‑101 (MQ=255)
                                                      tGTTCTGATTTTATCTTGCGGCAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCATCTCGGc                                         >  2:1936341/1‑101 (MQ=255)
                                                       gTTCTGATTTTATCTTGCGGCAAATATCCC.GGGGGGCGGGGCAGA.GCCCCGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCATCTCGGcc                                        <  1:4588282/101‑1 (MQ=255)
                                                       gTTCTGATTTTAACTTGCGGCAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCATCTCGGcc                                        >  1:2524868/1‑101 (MQ=255)
                                                        ttCTGATTTTATCTTGCGGCAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCATCTCGGCCa                                       <  1:1166673/101‑1 (MQ=255)
                                                         tCTGATTTTATCTTGCGGCAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCCTACAGCCCCTTGGGCTCGGCCGCATCCCAAAGCGCGTCCATCTCGGCCAg                                      >  1:3982899/1‑101 (MQ=38)
                                                         tCTGATTTTATCTTGCGGCAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCATCTCGGCCAg                                      <  1:2923821/101‑1 (MQ=255)
                                                          cTGATTTTATCTTGCGGCAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCATCTCGGCCAGa                                     <  1:3884687/101‑1 (MQ=255)
                                                          cTGATTTTATCTTGCGGCAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCATCTCGGCCAGa                                     >  1:1780525/1‑101 (MQ=255)
                                                            gATTTTATCTTGCGGCAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAACGCGCGTCCATCTCGGCCAGATc                                   >  2:3840659/1‑101 (MQ=255)
                                                            gATTTTATCTTGCGGCAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCATCTCGGCCAGATc                                   >  2:1189374/1‑101 (MQ=255)
                                                             aTTTTATCTTGCGGCAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCATCTCGGCCAGATCg                                  <  2:2587388/101‑1 (MQ=255)
                                                             aTTTTATCTTGCGGCAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCATCTCGGCCAGATCg                                  >  1:92504/1‑101 (MQ=255)
                                                              ttttATCTTGCGGCAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCATCTCGGCCAGATCGc                                 >  1:3102499/1‑101 (MQ=255)
                                                              ttttATCTTGCGGCAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCATCTCGGCCAGATCGc                                 >  2:4221493/1‑101 (MQ=255)
                                                               tttATCTTGCGGCAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCATCTCGGCCAGATCGCt                                >  1:1864715/1‑101 (MQ=255)
                                                                ttATCTTGCGGCAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCATCTCGGCCAGATCGCTg                               >  1:1250481/1‑101 (MQ=255)
                                                                 tATCTTGCGGCAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCATCTCGGCCAGATCGCTGt                              >  2:4583376/1‑101 (MQ=255)
                                                                 tATCTTGCGGCAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCATCTCGGCCAGATCGCTGt                              <  1:420572/101‑1 (MQ=255)
                                                                  aTCTTGCGGCAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCATCTCGGCCAGATCGCTGTc                             >  1:3783353/1‑101 (MQ=255)
                                                                   tCTGGCGGCAAATATCCC.GGGGGGCGGGGCAGA.GCCCCGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCATCTCGGCCAGATCGCTGTcg                            <  1:4525346/101‑1 (MQ=38)
                                                                    cTTGCGGCAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCATCTCGGCCAGATCGCTGTcgg                           <  1:3458373/101‑1 (MQ=255)
                                                                     ttGCGGCAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCATCTCGGCCAGATCGCTGTcggc                          >  1:3608405/1‑101 (MQ=255)
                                                                       ggggaaaaTATCCC.GGGGTGCGGGGCAGACC.CCCGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCATCTCGGCCAGATCGCTGTcggcgg                        <  2:3984902/96‑1 (MQ=38)
                                                                       gCGGCAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCATCTCGGCCAGATCGCTGTcggcgg                        <  1:3009378/101‑1 (MQ=255)
                                                                       gCGGCAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCATCTCGGCCAGATCGCTGTcggcgg                        >  2:2677087/1‑101 (MQ=255)
                                                                        cGGCAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCATCTCGGCCAGATCGCTGTCGGCggg                       >  2:1445908/1‑101 (MQ=255)
                                                                         ggCAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCATCTCGGCCAGATCGCTGTCGGCGGGc                      >  2:4111823/1‑101 (MQ=255)
                                                                          gCAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCATCTCGGCCAGATCGCTGTCGGCggggc                     >  1:1929625/1‑99 (MQ=255)
                                                                           gaaaTATCCCGGGGGTGGGGGCAAGACC.CCCGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCATCTCGGCCAGATCGCTGTCGGCGGGcc                     <  2:4024510/100‑1 (MQ=255)
                                                                           cAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCATCTCGGCCAGATCGCTGTCGGCGGGCCg                    >  1:979239/1‑101 (MQ=255)
                                                                           cAAATATCCC.GGGGTGCGGGGCAGA.GCCCCGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCATCTCGGCCAGATCGCTGTCGGCGGGCCg                    >  2:2358613/1‑101 (MQ=255)
                                                                           cAAATATCCC.GGGGCGCGGGGCAGA.GCCCCGCCTACAGCCCCTTGCGCTTGCCCGCATCCCAAAGCGCGTCCATCTCGGCCAGATCCCTGTCGGCGGGccc                    >  2:859703/1‑100 (MQ=38)
                                                                            aaaTATCCC.GGGGTGCGGGGCAGA.GCCCCGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCATCTCGGCCAGATCGCTGTCGGCGGGCCGc                   >  2:1525226/1‑101 (MQ=255)
                                                                            aaaTATCCC.GGGGTGCGGGGCAGA.GCCCCGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCATCTCGGCCAGATCGCTGTCGGCGGGCCGc                   >  1:1809402/1‑101 (MQ=255)
                                                                            aaaTATCCC.GGGGTGCGGGGCAGA.GCCCCGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCATCTCGGCCAGATCGCTGTCGGCGGGCCGc                   >  1:640882/1‑101 (MQ=255)
                                                                             aaTATCCC.GGGGTGCGGGGCAGA.GCCCCGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCATCTCGGCCAGATCGCTGTCGGCGGGCCGCt                  >  1:170067/1‑101 (MQ=255)
                                                                             aaTATCCC.GGGGTGCGGGGCAGA.GCCCCGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCATCTCGGCCAGATCGCTGTCGGCGGGCCGCt                  <  2:3102255/101‑1 (MQ=255)
                                                                              atttcgc.GGCGTTCGGGGCAGA.GCCCCGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCATCTCGGCCAGATCGCTGTCGGCGGGCCGCtt                 <  2:640821/95‑1 (MQ=255)
                                                                              atatCCC.GGGGTGCGGGGCAGA.GCCCCGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCATCTCGGCCAGATCGCTGTCGGCGGGCCGCtt                 >  1:364283/1‑101 (MQ=255)
                                                                               tatCCC.GGGGTGCGGGGCAGA.GCCCCGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCATCTCGGCCAGATCGCTGTCGGCGGGCCGCTTg                >  2:3508765/1‑101 (MQ=255)
                                                                               tatCCC.GGGGTGCGGGGCAGA.GCCCCGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCATCTCGGCCAGATCGCTGTCGGCGGGCCGCTTg                >  1:4037835/1‑101 (MQ=255)
                                                                               tacccc.GGGGTGCGGGGCAGACC.CCCGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCATCTCGGCCAGATCGCTGTCGGCGGGCCGCTTg                <  1:432914/98‑1 (MQ=255)
                                                                                atCCC.GGGGTGCGGGGCAGA.GCCCCGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCATCTCGGCCAGATCGCTGTCGGCGGGCCGCTTGc               >  1:1516719/1‑101 (MQ=255)
                                                                                    c.GGGGTGCGGGGCAGA.GCCCCGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCATCTCGGCCAGATCGCTGTCGGCGGGCCGCTTGCCGGc           >  2:1152789/1‑101 (MQ=255)
                                                                                    c.GGGGTGCGGGGCAGA.GCCCCGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCATCTCGGCCAGATCGCTGTCGGCGGGCCGCTTGCCGGc           >  2:624440/1‑101 (MQ=255)
                                                                                       gggtcgggggCAGA.GCCCCGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCATCTCGGCCAGATCGCTGTCGGCGGGCCGCTTGCCGGCCt         <  2:1134792/95‑1 (MQ=255)
                                                                                         gTGCGGGGCAGA.GCCCCGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCATCTCGGCCAGATCGCTGTCGGCGGGCCGCTTGCCGGCCTTc       <  1:2265071/101‑1 (MQ=255)
                                                                                             ggggCAGA.GCCCCGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCATCTCGGCCAGATCGCTGTCGGCGGGCCGCTTGCCGGCCTTCGCCa   >  1:4372426/1‑101 (MQ=255)
                                                                                             ggggCAGA.GCCCCGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCATCTCGGCCAGATCGCTGTCGGCGGGCCGCTTGCCGGCCTTCGCCa   >  2:2137424/1‑101 (MQ=255)
                                                                                              gggCAGA.GCCCCGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCATCTCGGCCAGATCGCTGTCGGCGGGCCGCTTGCCGGCCTTCGCCAg  <  2:1966358/101‑1 (MQ=255)
                                                                                                       |                                                                                            
GCCTCTTCGCAGGGTTTCTGATGCTGGTCGCGCTGAACATGCTGCGCAAGGCGCTGTTCTGATTTTATCTTGCGGCAAATATCCC.GGGGTGCGGGGCAGA.G.CCCGCCTACAGCCCCTTGCGCTTGGCCGCATCCCAAAGCGCGTCCATCTCGGCCAGATCGCTGTCGGCGGGCCGCTTGCCGGCCTTCGCCAG  >  NC_014034/1924999‑1925191

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG