BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,948,851	+G	89.3%	coding (1069/2472 nt)	quiP ←	acyl‑homoserine‑lactone acylase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,948,851	1	.→G	89.3%	69.3 ‑0.7 ‑0.7	214	coding (1069/2472 nt)	quiP	acyl‑homoserine‑lactone acylase
Reads supporting (aligned to +/- strand): new base (98/93): ref base (10/13): total (108/106)
Fisher's exact test strand distribution p-value = 5.15e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.81e-01

Only 100 of 225 total aligned reads displayed.


TGATCCGACGGCAGCACCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTC.GC.C.C.ACCCTCGGGGCTGCGGTA.CGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGC  >  NC_014034/1948751‑1948943


 gATCCGACGGCAGCACCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTC.g                                                                                                 <  2:3805757/101‑1 (MQ=255)


 gATCCGACGGCAGCACCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTC.g                                                                                                 >  2:2493699/1‑101 (MQ=255)


  aTCCGACGGCAGCACCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTC.gg                                                                                                >  1:1582872/1‑100 (MQ=255)


   tCCGACGGCAGCACCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTC.ggc                                                                                               >  2:4132645/1‑99 (MQ=255)


    ccGACGGCAGCACCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTC.Gg.c.c                                                                                            >  2:1195203/1‑101 (MQ=255)


    ccGACGGCAGCACCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTC.Gg.c.c                                                                                            <  1:2538980/101‑1 (MQ=255)


      gACGGCAGCACCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTC.Gg.c.cca                                                                                          >  1:1556867/1‑99 (MQ=255)


      gACGGCAGCACCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTC.Gg.c.cca                                                                                          >  2:1535181/1‑99 (MQ=255)


      gACGGCAGCACCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTC.GG.C.C.aa                                                                                         >  2:3573970/1‑100 (MQ=255)


       aCGGCAGCACCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTC.Gg.c.ccaa                                                                                         >  2:303913/1‑98 (MQ=255)


          gcagcaCCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTC.Gg.c.ccaaccc                                                                                      <  2:863988/101‑7 (MQ=255)


          gcagcaCCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTC.Gg.c.ccaaccc                                                                                      <  2:2482048/101‑7 (MQ=255)


          gcagcaCCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTC.Gg.c.ccaaccc                                                                                      <  1:4506986/101‑7 (MQ=255)


            agcaCCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTC.Gg.c.ccaaccctc                                                                                    <  2:1424265/101‑9 (MQ=255)


            agcaCCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTC.Gg.c.ccaaccctc                                                                                    >  1:969621/1‑93 (MQ=255)


            agcaCCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTC.Gg.c.ccaaccctc                                                                                    <  1:783156/101‑9 (MQ=255)


              caCCGGGCCTTTTTCGGTCGAGCTGAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.C.CAACCCTCgg                                                                                   <  1:2363194/101‑1 (MQ=255)


                ccGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.C.CAACCCTCgggg                                                                                 >  2:561933/1‑101 (MQ=255)


                 cGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.C.CAACCCTCGGGGc                                                                                >  2:3371743/1‑101 (MQ=255)


                 cGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.C.CAACCCTCGGGGc                                                                                >  1:3711307/1‑101 (MQ=255)


                   ggCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.C.CAACCCTCGGGGCTg                                                                              <  2:3641741/101‑1 (MQ=255)


                    gCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.C.CAACCCTCGGGGCTGc                                                                             <  1:2046592/101‑1 (MQ=255)


                     gcGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.C.CAACCCTCGGGGCTGCg                                                                            <  2:1118393/100‑1 (MQ=255)


                     ccGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.C.CAACCCTCGGGGCTGCg                                                                            >  2:222389/1‑101 (MQ=255)


                     ccGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCGTGCGGGTGGTGAACTCGGC.C.CAACCCTCGGGGCTGCg                                                                            >  1:3246214/1‑101 (MQ=255)


                       gTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.C.CAACCCTCGGGGCTGCGGt                                                                          <  2:189353/101‑1 (MQ=255)


                       gTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.C.CAACCCTCGGGGCTGCGGt                                                                          >  2:276323/1‑101 (MQ=255)


                       gTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.C.CAACCCTCGGGGCTGCGGt                                                                          <  2:1636842/101‑1 (MQ=255)


                       gTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.C.CAACCCTCGGGGCTGCGGt                                                                          <  1:190893/101‑1 (MQ=255)


                        ttttCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.C.CAACCCTCGGGGCTGCGGTa                                                                         <  1:3649890/101‑1 (MQ=255)


                        ttttCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.C.CAACCCTCGGGGCTGCGGTa                                                                         >  2:3454408/1‑101 (MQ=255)


                        gtttCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.C.CAACCCTCGGGGCTGCGGTa                                                                         <  1:1489068/100‑1 (MQ=255)


                          ttCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.C.CAACCCTCGGGGCTGCGGTagc                                                                       >  1:3207408/1‑99 (MQ=255)


                            cGGTCCAGCGCAGGGTGAGGGTGATTGGCGCGGCATCCTTGGCCATGAGGATAATTTTGCGGGGGGGGAGCTC.GC.CTCAACCCTCGGGGCTGCGGTA.ccgc                                                                    >  1:3863177/1‑98 (MQ=255)


                            cGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.C.CAACCCTCGGGGCTGCGGTagcgc                                                                     >  2:492467/1‑97 (MQ=255)


                               tCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.C.CAACCCTCGGGGCTGCGGTAGCGCTCg                                                                  <  1:1195295/101‑1 (MQ=255)


                               tCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.C.CAACCCTCGGGGCTGCGGTAGCGCTCg                                                                  >  2:800510/1‑101 (MQ=255)


                               gccAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.C.CAACCCTCGGGGCTGCGGTAGCGCTCg                                                                  <  1:3452724/100‑1 (MQ=255)


                                  aGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.C.CAACCCTCGGGGCTGCGGTAGCGCTCGGGc                                                               >  2:1968255/1‑101 (MQ=255)


                                    cgcAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.C.CAACCCTCGGGGCTGCGGTAGCGCTCGGGCGt                                                             <  2:172477/101‑1 (MQ=255)


                                     tcAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.C.CAACCCTCGGGGCTGCGGTAGCGCTCGGGCGtt                                                            <  1:4359395/100‑1 (MQ=255)


                                     gcAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.C.CAACCCTCGGGGCTGCGGTAGCGCTCGGGCgct                                                            >  2:4510772/1‑99 (MQ=255)


                                     gcAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.C.CAACCCTCGGGGCTGCGGTAGCGCTCGGGCGtt                                                            <  2:374463/101‑1 (MQ=255)


                                      cAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.C.CAACCCTCGGGGCTGCGGTAGCGCTCGGGCGtta                                                           >  2:4359103/1‑100 (MQ=255)


                                      cAGGGTGAGGGTGATCGGCGCGGCAGCCTTTTCCGTGACGATCACCTTGCGTGTGGTGAACTCGGC.C.CAACCCTCGGGGCTGCGGTAGCGCTCGGGCGttt                                                           <  1:545223/101‑1 (MQ=255)


                                       agggtgagggtgATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.C.CAACCCTCGGGGCTGCGGTAGCGCTCGGGCGtttt                                                          >  2:3938271/1‑101 (MQ=255)


                                       agggtgagggtgATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.C.CAACCCTCGGGGCTGCGGTAGCGCTCGGGCGtttt                                                          >  1:827997/1‑101 (MQ=255)


                                       agggtgagggtgATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.C.CAACCCTCGGGGCTGCGGTAGCGCTCGGGCGtttt                                                          >  1:3531067/1‑101 (MQ=255)


                                       agggtgagggtgATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.C.CAACCCTCGGGGCTGCGGTAGCGCTCGGGCGtttt                                                          <  1:2870459/101‑1 (MQ=255)


                                        gggtgagggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.C.CAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTc                                                         <  1:2177087/101‑1 (MQ=255)


                                         ggtgagggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.C.CAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCg                                                        >  1:2822675/1‑101 (MQ=255)


                                          gtgagggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.C.CAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCgg                                                       <  1:4590957/101‑1 (MQ=255)


                                             agggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGCTC.A.ACCCTCGGGCATGCGGTAGCGCTCGGGCGTCTTCGGGTc                                                    >  1:2310665/1‑101 (MQ=255)


                                             agggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.C.CAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATc                                                    >  2:2410187/1‑101 (MQ=255)


                                              gggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.C.CAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCa                                                   >  2:3107734/1‑101 (MQ=255)


                                               ggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.C.CAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCaa                                                  <  1:3312781/101‑1 (MQ=255)


                                                 tgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.C.CAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGt                                                >  2:3346285/1‑101 (MQ=255)


                                                   aTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.C.CAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTc                                              <  2:2944437/101‑1 (MQ=255)


                                                   aTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.C.CAACCCTCAGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTc                                              >  2:2745254/1‑101 (MQ=255)


                                                      ggCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.C.CAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTc                                           <  1:1003814/101‑1 (MQ=255)


                                                      ggCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.C.CAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTc                                           <  1:4127360/101‑1 (MQ=255)


                                                       gcgcGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.C.CAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTcg                                          >  1:4482967/1‑101 (MQ=255)


                                                        cgcgGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.C.CAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAgaaatcgga                                         <  2:4669035/101‑9 (MQ=255)


                                                          cgGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.C.CAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTcgacg                                       <  2:2028806/101‑1 (MQ=255)


                                                           ggCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.C.CAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTcgacga                                      <  2:359776/101‑1 (MQ=255)


                                                            gCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.C.CAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTGGACGaa                                     >  1:4330041/1‑101 (MQ=255)


                                                            gCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.C.CAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGaa                                     >  2:1755630/1‑101 (MQ=255)


                                                            gCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.C.CAACCCTCGGGGCTGCGGGAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGaa                                     >  1:1338101/1‑101 (MQ=255)


                                                             cATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.C.CAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAAc                                    <  1:2493865/101‑1 (MQ=255)


                                                              aTCCTTGACCGTGCCGATCACCTTGCGGGTGGTGAACTCGGC.C.CAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACa                                   <  1:3107978/101‑1 (MQ=255)


                                                              aTCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.C.CAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACa                                   >  2:1604694/1‑101 (MQ=255)


                                                              aTCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.C.CAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACa                                   <  2:3542887/101‑1 (MQ=255)


                                                               tCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.C.CAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAAccg                                  >  1:853838/1‑99 (MQ=255)


                                                               tCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.C.CAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAg                                  >  2:1314648/1‑101 (MQ=255)


                                                                 cTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.C.CAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAggc                                >  2:592835/1‑100 (MQ=255)


                                                                 cTTGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.C.CAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGt                                >  2:2614802/1‑101 (MQ=255)


                                                                  ttGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.C.CAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTc                               <  1:2270491/101‑1 (MQ=255)


                                                                   tGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.C.CAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTcc                              >  1:1877008/1‑101 (MQ=255)


                                                                   tGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.C.CAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTcc                              <  2:3409746/101‑1 (MQ=255)


                                                                   tGACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.C.CAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTcc                              <  2:2532785/101‑1 (MQ=255)


                                                                    gACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.C.CAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCt                             <  2:460980/101‑1 (MQ=255)


                                                                    gACCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.C.CAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCt                             <  2:2701191/101‑1 (MQ=255)


                                                                     aCCGTGACGATCACCTTTCGGGTGGTGAACTCGGC.C.CAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTc                            <  2:4185869/101‑1 (MQ=255)


                                                                     aCCGTGACGATCACCTTGCGGGTGGTGAACTCGGC.C.CAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTc                            <  2:1857045/101‑1 (MQ=255)


                                                                       cGTGACGATCACCTTGCGGGTGGTGAACTCGGC.C.CAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGt                          >  1:1029920/1‑101 (MQ=255)


                                                                         tGACGATCACCCTGCGGGTGGTGAACTCGGCTC.A.ACCCTCGGTGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCa                        <  2:2310503/101‑1 (MQ=255)


                                                                          gACGATCACCTTGCGGGTGGTGAACTCGGC.C.CAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCAt                       >  1:472281/1‑101 (MQ=255)


                                                                             gATCACCTTGCGGGTGGTGAACTCGGC.C.CAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCgg                    >  2:2129697/1‑101 (MQ=255)


                                                                               tCACCTTGCGGGTGGTGAACTCGGC.C.CAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCg                  <  2:4170746/101‑1 (MQ=255)


                                                                               tCACCTTGCGGGTGGTGAACTCGGC.C.CAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCg                  <  1:462269/101‑1 (MQ=255)


                                                                               tCACCTTGCGGGTGGTGAACTCGGC.C.CAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCg                  >  1:3585923/1‑101 (MQ=255)


                                                                               tCACCTTGCGGGTGGTGAACTCGGC.C.CAACCCTCGGGGCTGAGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCg                  <  1:3391500/101‑1 (MQ=255)


                                                                                   cTTGCGGGTGGTGAACTCGGC.C.CAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAgg              >  1:4198327/1‑101 (MQ=255)


                                                                                     tGCGGGTGGTGAACTCGGC.C.CAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAggcg            <  1:587935/101‑1 (MQ=255)


                                                                                       cGGGTGGTGAACTCGGC.C.CAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATTAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAggcggc          >  2:1835360/1‑101 (MQ=255)


                                                                                        gggTGGTGAACTCGGC.C.CAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGgcggcg         >  1:1664412/1‑101 (MQ=255)


                                                                                          gtggtgAACTCGGC.C.CAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGt       <  2:3138152/101‑1 (MQ=255)


                                                                                          gatctgAACTCGGC.C.CAACCCGCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGt       <  1:2237125/97‑1 (MQ=255)


                                                                                               gAACTCGGC.C.CAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGc  >  2:40874/1‑101 (MQ=255)


                                                                                               gAACTCGGC.C.CAACCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGc  >  1:3766613/1‑101 (MQ=255)


TGATCCGACGGCAGCACCGGGCCGTTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACTC.GC.C.C.ACCCTCGGGGCTGCGGTA.CGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGC  >  NC_014034/1948751‑1948943

Base quality scores:


ATCG

< 3 ≤


ATCG

< 20 ≤


ATCG

< 27 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG