Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,948,873 +G 100% coding (1047/2472 nt) quiP ← acyl‑homoserine‑lactone acylase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,948,8731.→G100.0% 738.4 195coding (1047/2472 nt)quiPacyl‑homoserine‑lactone acylase
Reads supporting (aligned to +/- strand):  new base (97/98):  ref base (0/0):  total (97/98)

Only 100 of 216 total aligned reads displayed.

TTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.C.GCC.C.A.CCCTCGGGGCTGCGGTA.CGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAGCCAAGCTGGTCGCTGCGCC  >  NC_014034/1948775‑1948967
                                                                                                        |                                                                                              
ttttCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCC.CAA.CCCTCGGGGCTGCGGTa                                                                                                 >  2:898843/1‑101 (MQ=255)
ttttCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCC.CAA.CCCTCGGGGCTGCGGTa                                                                                                 >  2:3454408/1‑101 (MQ=255)
gtttCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCC.CAA.CCCTCGGGGCTGCGGTa                                                                                                 <  1:1489068/100‑1 (MQ=255)
 tttCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCC.CAA.CCCTCGGGGCTGCGGTag                                                                                                >  1:4273695/1‑100 (MQ=255)
   tCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCC.CAA.CCCTCGGGGCTGCGGTagcg                                                                                              >  2:4180546/1‑98 (MQ=255)
    cGGTCCAGCGCAGGGTGAGGGTGATTGGCGCGGCATCCTTGGCCATGAGGATAATTTTGCGGGGGGGGAGCT.C.GCCTCAA.CCCTCGGGGCTGCGGTA.ccgc                                                                                            >  1:3863177/1‑98 (MQ=255)
     ggTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCC.CAA.CCCTCGGGGCTGCGGTagcgct                                                                                            >  1:1393583/1‑96 (MQ=255)
      gTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCC.CAA.CCCTCGGGGCTGCGGTAGCGCTc                                                                                           >  2:3516025/1‑101 (MQ=255)
       gccAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCC.CAA.CCCTCGGGGCTGCGGTAGCGCTCg                                                                                          <  1:3452724/100‑1 (MQ=255)
        ccAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCC.CAA.CCCTCGGGGCTGCGGTAGCGCTCgg                                                                                         >  1:4338452/1‑101 (MQ=255)
          ggcgcAGGGTGAGGGTGATCGGCGCGGTATCCTTGACCGTGACGCTCACATTGCGGGTGGTGAACT.CGGCC.CAA.CCCTCGGGGCTGCGGTAGCGCTCGGGc                                                                                       <  2:1564193/100‑1 (MQ=255)
          aGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCC.CAA.CCCTCGGGGCTGCGGTAGCGCTCGGGc                                                                                       <  1:521645/101‑1 (MQ=255)
          aGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCC.CAA.CCCTCGGGGCTGCGGTAGCGCTCGGGc                                                                                       <  2:1920138/101‑1 (MQ=255)
            cgcAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCC.CAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGt                                                                                     <  2:172477/101‑1 (MQ=255)
            cgcAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCC.CAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGt                                                                                     >  1:2421760/1‑101 (MQ=255)
             gcAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCC.CAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCgct                                                                                    >  2:4510772/1‑99 (MQ=255)
             gcAGGGTGAGGGTGATCAGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCC.CAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGtt                                                                                    <  2:4167054/101‑1 (MQ=255)
              cAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCC.CAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGtta                                                                                   >  2:4359103/1‑100 (MQ=255)
               agggtgagggtgATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCC.CAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGtttt                                                                                  >  2:3313992/1‑101 (MQ=255)
               agggtgagggtgATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCC.CAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGtttt                                                                                  >  1:827997/1‑101 (MQ=255)
               agggtgagggtgATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCC.CAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGtttt                                                                                  >  1:2119961/1‑101 (MQ=255)
                gggtgagggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCC.CAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTc                                                                                 <  1:2177087/101‑1 (MQ=255)
                gggtgagggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCC.CAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTc                                                                                 >  2:3358041/1‑101 (MQ=255)
                    gagggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCC.CAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGAt                                                                             <  2:757168/101‑1 (MQ=255)
                     agggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCC.CAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATc                                                                            >  2:2410187/1‑101 (MQ=255)
                     agggtgaTCGGCGCGGCATCCTTGACCGTGACGATAACCTTGCGGGTGATGAACTCC.GCC.C.AGCCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATc                                                                            <  2:3071478/101‑1 (MQ=255)
                      gggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCC.CAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCa                                                                           <  1:4427358/101‑1 (MQ=255)
                      gggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCC.CAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCa                                                                           <  2:3951392/101‑1 (MQ=255)
                       ggtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCC.CAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCaa                                                                          <  1:3312781/101‑1 (MQ=255)
                        gtgaTTGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCC.CAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAg                                                                         >  2:1693434/1‑101 (MQ=255)
                        gtgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCC.CAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAg                                                                         <  2:2364758/101‑1 (MQ=255)
                         tgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCC.CAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGt                                                                        >  2:4245399/1‑101 (MQ=255)
                         tgaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCC.CAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGt                                                                        >  1:3614916/1‑101 (MQ=255)
                          gaTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCC.CAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGtt                                                                       <  2:2488882/101‑1 (MQ=255)
                           aTCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCC.CAA.CCCTCAGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTc                                                                      >  2:2745254/1‑101 (MQ=255)
                             cGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCC.CAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCt                                                                    <  1:3665321/101‑1 (MQ=255)
                             cGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCC.CAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCt                                                                    <  1:2287570/101‑1 (MQ=255)
                              ggCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCC.CAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTc                                                                   <  2:592117/101‑1 (MQ=255)
                              ggCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCC.CAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTc                                                                   <  1:1003814/101‑1 (MQ=255)
                               gcgcGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCC.CAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTcg                                                                  <  2:497700/101‑1 (MQ=255)
                               gcgcGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCC.CAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTcg                                                                  >  1:4482967/1‑101 (MQ=255)
                                cgcgGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCC.CAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAgaaatcgga                                                                 <  2:4669035/101‑9 (MQ=255)
                                 gcgGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCC.CAA.CTCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTcgac                                                                <  1:2833276/101‑1 (MQ=255)
                                  cgGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCC.CAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTcgacg                                                               <  2:2028806/101‑1 (MQ=255)
                                  cgGCATCCTTGACCGTGACGATAACCTTGCGGGTGGTGAACT.CGGCC.CAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTcgacg                                                               >  2:1178803/1‑101 (MQ=255)
                                    gCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCC.CAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGaa                                                             >  2:1755630/1‑101 (MQ=255)
                                     cATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCC.CAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAAc                                                            <  2:858566/101‑1 (MQ=255)
                                     cATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCC.CAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAAc                                                            <  1:2493865/101‑1 (MQ=255)
                                      aTCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCC.CAA.CCCTCGGGGCTGCGGTAGCGCTCGGGGGGTTTTTGGTTAAGTTACTcttcctaca                                                           >  2:3717215/1‑93 (MQ=255)
                                      aTCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCC.CAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACa                                                           >  2:1604694/1‑101 (MQ=255)
                                       tCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCC.CAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAg                                                          >  2:1314648/1‑101 (MQ=255)
                                         cTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCC.CAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGt                                                        >  2:2614802/1‑101 (MQ=255)
                                           tGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCC.CAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTcc                                                      >  1:1877008/1‑101 (MQ=255)
                                           tGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCC.CAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTcc                                                      <  2:3409746/101‑1 (MQ=255)
                                           tGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCC.CAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTcc                                                      >  1:3648403/1‑101 (MQ=255)
                                           tGACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCC.CAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTcc                                                      <  2:1070350/101‑1 (MQ=255)
                                            gACCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCC.CAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCt                                                     <  2:2701191/101‑1 (MQ=255)
                                             aCCGTGACGATCACCTTGCGGGTGGTGAACT.CGGCC.CAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTc                                                    <  2:1857045/101‑1 (MQ=255)
                                              ccGTGACGATCACCTTGCGGGTGGTGAACT.CGGCC.CAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCg                                                   <  2:3592751/101‑1 (MQ=255)
                                               cGTGACGATCACCTTGCGGGTGGTGAACT.CGGCC.CAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGt                                                  >  1:1029920/1‑101 (MQ=255)
                                                  gACGATCACCTTGCGGGTGGTGAACT.CGGCC.CAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCAt                                               >  2:4106702/1‑101 (MQ=255)
                                                  gACGATCACCTTGCGGGTGGTGAACT.CGGCC.CAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCAt                                               >  1:472281/1‑101 (MQ=255)
                                                   aCGATCACCTTGCGGGTGGTGAACT.CGGCC.CAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATc                                              <  1:3548683/101‑1 (MQ=255)
                                                    cGATCACCTTGCGGGTGGTGAACT.CGGCC.CAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCg                                             >  2:3122554/1‑101 (MQ=255)
                                                     gATCACCTTGCGGGTGGTGAACT.CGGCC.CAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCgg                                            >  2:2129697/1‑101 (MQ=255)
                                                       tCACCTTGCGGGTGGTGAACT.CGGCC.CAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCg                                          >  2:4545666/1‑101 (MQ=255)
                                                         aCCTTGCGGGTGGTGAACT.CGGCC.CAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGaa                                        >  1:1564536/1‑101 (MQ=255)
                                                         aCCTTGCGGGGGGTGAACT.CGGCC.CAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGaa                                        <  1:1968390/101‑1 (MQ=255)
                                                            ttGCGGGTGGTGAACT.CGGCC.CAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAggc                                     <  1:893668/101‑1 (MQ=255)
                                                             tGCGGGTGGTGAACT.CGGCC.CAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAggcg                                    <  1:587935/101‑1 (MQ=255)
                                                              gCGGGTGGTGAACT.CGGCC.CAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAggcgg                                   <  1:222413/101‑1 (MQ=255)
                                                               cGGGTGGTGAACT.CGGCC.CAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATTAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAggcggc                                  >  2:1835360/1‑101 (MQ=255)
                                                               cGGGTGGTGAACT.CGGCC.CAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAggcggc                                  >  2:354573/1‑101 (MQ=255)
                                                               cGGGTGGTGAACT.CGGCC.CAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAggcggc                                  >  1:2929759/1‑101 (MQ=255)
                                                                gggTGGTGAACT.CGGCC.CAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGgcggcg                                 >  1:1664412/1‑101 (MQ=255)
                                                                 ggtggtGAACT.CGGCC.CAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGcggcgg                                >  2:2579543/1‑101 (MQ=255)
                                                                    ggtgAACT.CGGCC.CAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGa                             >  1:4641675/1‑101 (MQ=255)
                                                                    ggtgAACT.CGGCC.CAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGa                             <  2:3766853/101‑1 (MQ=255)
                                                                    ggtgAACT.CGGCC.CAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCCAAGGCGGCGGTGa                             >  2:4403405/1‑101 (MQ=255)
                                                                      tgAACT.CGGCC.CAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATg                           >  2:2012630/1‑101 (MQ=255)
                                                                       gAACT.CGGCC.CAA.CCTTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGGGATGc                          >  1:3934005/1‑101 (MQ=255)
                                                                       gAACT.CGGCC.CAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGc                          >  1:3766613/1‑101 (MQ=255)
                                                                       gAACT.CGGCC.CAA.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGGCCTCGTCATCGGCGAAGGtggcgggagctc                          >  2:2236963/1‑95 (MQ=255)
                                                                            ctcggcccaa.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGcccc                       <  1:2787587/92‑1 (MQ=255)
                                                                              cggcccaa.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAg                     <  1:4604439/94‑1 (MQ=255)
                                                                              cggcccaa.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAg                     <  1:3342583/94‑1 (MQ=255)
                                                                                gcccaa.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAGcc                   <  1:3188489/96‑1 (MQ=255)
                                                                                  ccaa.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGGGATGCCCCAGCCaa                 >  2:4623557/4‑101 (MQ=255)
                                                                                   caa.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAGCCAAg                <  1:1726220/99‑1 (MQ=255)
                                                                                   caa.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAGCCAAg                >  2:2918947/3‑101 (MQ=255)
                                                                                   caa.CCCTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAGCCAAg                >  1:4532485/3‑101 (MQ=255)
                                                                                         cTCGGGGCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAGCCAAGCTggc           >  1:124395/1‑100 (MQ=255)
                                                                                            ggggCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAGCCAAGCTGGTCGc        >  1:2315685/1‑101 (MQ=255)
                                                                                             gggCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAGCCAAGCTGGTCGCt       <  2:827921/101‑1 (MQ=255)
                                                                                              ggCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAGCCAAGCTGGTCGCTg      >  1:4170914/1‑101 (MQ=255)
                                                                                              ggCTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAGCCAAGCTGGTCGCTg      >  1:548670/1‑101 (MQ=255)
                                                                                              ggCTGCCGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAGCCAAGCTGGTCGCTg      <  2:3884982/101‑1 (MQ=255)
                                                                                                cTGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAGCCAAGCTGGTCGCTgcg    <  1:3387742/101‑1 (MQ=255)
                                                                                                 tGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAGCCAAGCTGGTCGCTgcgc   <  2:3702556/101‑1 (MQ=255)
                                                                                                 tGCGGTAGCGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAGCCAAGCTGGTCGCTgcgc   >  2:3633232/1‑101 (MQ=255)
                                                                                                        |                                                                                              
TTTTCGGTCCAGCGCAGGGTGAGGGTGATCGGCGCGGCATCCTTGACCGTGACGATCACCTTGCGGGTGGTGAACT.C.GCC.C.A.CCCTCGGGGCTGCGGTA.CGCTCGGGCGTTTTCGGATCAAGTTCCTCGACGAACAGGTCCTCGTCATCGGCGAAGGCGGCGGTGATGCCCCAGCCAAGCTGGTCGCTGCGCC  >  NC_014034/1948775‑1948967

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 28 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG