Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,974,330 +G 100% intergenic (‑23/+32) rpe1 ← / ← cbbM ribulose‑phosphate 3‑epimerase/ribulose bisphosphate carboxylase large subunit

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,974,3301.→G100.0% 636.9 172intergenic (‑23/+32)rpe1/cbbMribulose‑phosphate 3‑epimerase/ribulose bisphosphate carboxylase large subunit
Reads supporting (aligned to +/- strand):  new base (83/87):  ref base (1/0):  total (84/88)

Only 100 of 199 total aligned reads displayed.

TTTCACGGCCGAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTT.GGGC..GGGGTG.T.C.CCCCGCCCGGATAGCACCGGCGTTCAGTTAACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGG  >  NC_014034/1974235‑1974419
                                                                                                 |                                                                                             
  tCACGGCCGAAATCGGCGAGGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAGCGGTCGAAGGTGATGTCGGTCTCCTGAGGGGTTGGGGC..gggggt                                                                                        <  2:3265153/101‑3 (MQ=255)
  tCACGGCCGAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGGCATGTCGGTCTCCTGCGGGGTTGGGGC..gggggg                                                                                        >  2:2570197/1‑99 (MQ=255)
  tCACGGCCGAAAGCGGCGAAGTCGGCAGAAAAGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGACATGTCGGTCTCCTGAGGGGTTGGGGC..gggggt                                                                                        <  2:3639923/101‑3 (MQ=255)
   cACGGCCGAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGC..ggggggg                                                                                       >  1:1973675/1‑98 (MQ=255)
     cGGCCGAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGC..gggggtgtc                                                                                     <  1:3411678/101‑6 (MQ=255)
      ggCCGGAATCGGCGGAATCGGCAGAAAGGCTCGAGGTGTCGATCTTGACGGAACGGTCGAAGGTCATGTCGGTTTCCTGAGGGGTTGGGGCG.GGGGTG.T.c.c                                                                                  <  2:112591/101‑1 (MQ=255)
      ggCCGAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCG.GGGGTG.T.c.c                                                                                  <  1:3258765/101‑1 (MQ=255)
       gCCGAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCG.GGGGTG.T.c.cc                                                                                 <  2:4412696/101‑1 (MQ=255)
       gCCGAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGC..gggggtggccc                                                                                   >  1:2855485/1‑94 (MQ=255)
        ccGAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCG.GGGGTG.T.c.ccc                                                                                <  2:852094/101‑1 (MQ=255)
         cGAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCG.GGGGGG.T.c.cccc                                                                               >  1:4396082/1‑101 (MQ=255)
          gAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCG.GGGGTG.T.c.ccccc                                                                              >  2:3885022/1‑100 (MQ=255)
          gAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCG.GGGGTG.T.c.ccccc                                                                              <  1:2084156/101‑2 (MQ=255)
          gAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGC..ggggggggcccccc                                                                                >  1:1860551/1‑91 (MQ=255)
              tCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCG.GGGGTG.T.c.cccccgccc                                                                          <  1:1428546/101‑6 (MQ=255)
               cGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCG.GGGGGG.TCC.Ccccgcccg                                                                          >  2:3048150/1‑101 (MQ=255)
               cGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCG.GGGGGG.TCC.Ccccgcccg                                                                          >  1:4572081/1‑101 (MQ=255)
                ggCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCG.GGGGTG.TCC.CCCCGCCCgg                                                                         <  2:2544280/101‑1 (MQ=255)
                  cGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCG.GGGGGG.TCC.CCCCGCCCGGag                                                                       >  2:1394912/1‑100 (MQ=255)
                   gAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCG.GGGGTG.TCC.CCCCGCCCGGATa                                                                      >  1:2315578/1‑101 (MQ=255)
                    aaGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCG.GGGGTG.TCC.CCCCGCCCGGATAg                                                                     <  2:871860/101‑1 (MQ=255)
                    aaGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTT.GGGCCGGGGGTG.TCC.CCCCGCCCGGATAg                                                                     <  1:3009985/101‑1 (MQ=255)
                     aGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCG.GGGGTG.TCC.CCCCGCCCGGATAGc                                                                    >  1:2078582/1‑101 (MQ=255)
                        cGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCG.GGGGTG.TCC.CCCCGCCCGGATAGCAcg                                                                 <  2:4106898/101‑2 (MQ=255)
                        cGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCG.GGGGTG.TCC.CCCCGCCCGGATAGCAcg                                                                 <  2:2837011/101‑2 (MQ=255)
                        cGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCG.GGGGGG.TCC.CCCCGCCCGGATAGCAcg                                                                 >  2:1512951/1‑100 (MQ=255)
                         ggCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCG.GGGGGG.TAC.CCCCCCCCGGGTAGCaggg                                                                >  2:3525547/1‑98 (MQ=255)
                          gCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCG.GGGGTG.TCC.CCCCGCCCGGATAGCAcggc                                                               <  1:1306457/101‑4 (MQ=255)
                          gCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGCCGGTCTCCTGGGGGGTTGGGGC..GGGGGGCG.CTCCCCGCCCTGATAGCACGgg                                                               >  2:1087133/1‑101 (MQ=255)
                           cAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCG.GGGGTG.TCC.CCCCGCCCGGATAGCAcggcg                                                              >  2:24960/1‑97 (MQ=255)
                             gAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCG.GGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGtt                                                           >  1:1710717/1‑101 (MQ=255)
                              aaaGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCG.GGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTc                                                          >  1:2478336/1‑101 (MQ=255)
                               aaGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCG.GGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCa                                                         <  2:161613/101‑1 (MQ=255)
                               aaGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCG.GGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCa                                                         >  2:2012286/1‑101 (MQ=255)
                               aaGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCG.GGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCa                                                         <  2:2207072/101‑1 (MQ=255)
                               aaGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCG.GGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCa                                                         <  2:3721985/101‑1 (MQ=255)
                                aGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCG.GGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAg                                                        <  2:4494917/101‑1 (MQ=255)
                                 ggATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCG.GGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGt                                                       <  2:4589339/101‑1 (MQ=255)
                                  gATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCG.GGGGTG.TCC.CCCCGCCCGGATAGGA.CGGCGTTCAGtt                                                      >  2:869009/1‑101 (MQ=255)
                                  gATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCG.GGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGtt                                                      <  1:112811/101‑1 (MQ=255)
                                  gATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCG.GGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGtt                                                      <  2:4358347/101‑1 (MQ=255)
                                  gATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCG.GGGGGG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGtt                                                      >  1:1239593/1‑101 (MQ=255)
                                    tCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCG.GGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGttca                                                    <  1:1035075/101‑3 (MQ=255)
                                     cGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCG.GGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCAc                                                   <  1:4333589/101‑1 (MQ=255)
                                     cGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCG.GGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCAc                                                   >  1:1354259/1‑101 (MQ=255)
                                     cGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCG.GGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCAc                                                   >  1:2216559/1‑101 (MQ=255)
                                      gAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCG.GGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACg                                                  >  1:1941255/1‑101 (MQ=255)
                                      gAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCG.GGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACg                                                  >  1:260484/1‑101 (MQ=255)
                                      gAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCG.GGGGGG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGGTCACg                                                  >  1:3710824/1‑101 (MQ=255)
                                      gAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCG.GGGGGG.TCC.CCCCGCCCGGATAG.AACGGCGTTCAGTTCACg                                                  >  2:3975878/1‑101 (MQ=255)
                                       aGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCG.GGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGc                                                 >  1:2235393/1‑101 (MQ=255)
                                           ggCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCG.GGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCag                                             <  1:4070817/101‑1 (MQ=255)
                                            gCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCG.GGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCcga                                            >  1:1000408/1‑101 (MQ=255)
                                            gCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCG.GGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCaga                                            <  2:4472670/101‑1 (MQ=255)
                                            gCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCG.GGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCaga                                            >  2:1903850/1‑101 (MQ=255)
                                             cGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCG.GGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCagag                                           >  1:1051707/1‑101 (MQ=255)
                                              gATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCG.GGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGc                                          >  2:1202656/1‑101 (MQ=255)
                                              gATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCG.GGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGc                                          <  2:3037499/101‑1 (MQ=255)
                                              gATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCG.GGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGc                                          <  1:1526286/101‑1 (MQ=255)
                                               aTCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCG.GGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCg                                         >  1:611001/1‑101 (MQ=255)
                                                tCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCG.GGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCCGAGCGa                                        >  2:1794570/1‑101 (MQ=255)
                                                tCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCG.GGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGa                                        >  1:283764/1‑101 (MQ=255)
                                                tCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCG.GGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGa                                        <  2:1000325/101‑1 (MQ=255)
                                                  ttGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCG.GGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGAcg                                      <  1:4189831/101‑1 (MQ=255)
                                                  ttGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCG.GGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGAcg                                      <  2:2681593/101‑1 (MQ=255)
                                                   tGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCG.GGGGTT.TGC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGAcgc                                     >  2:2992314/1‑101 (MQ=255)
                                                    gATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCG.GGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGAcgcg                                    <  2:2707430/101‑1 (MQ=255)
                                                     aTGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCG.GGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACgcgc                                   >  2:4042864/1‑101 (MQ=255)
                                                      tGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCG.GGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGcc                                  <  1:3599156/101‑1 (MQ=255)
                                                       gtaaCGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCG.GGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCa                                 >  1:703216/3‑101 (MQ=255)
                                                       ggAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCG.GGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCa                                 <  2:2161070/101‑1 (MQ=255)
                                                          aCGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCG.GGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGcc                              >  1:507399/1‑101 (MQ=255)
                                                          aCGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCG.GGGGTG.TCC.CCCCCCCCGGATAGCA.CGACGTTCAGTTCACGCCCAGAGCGACGCGCCAGcc                              >  1:4010499/1‑101 (MQ=255)
                                                             gTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCG.GGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCgg                           <  1:2910539/101‑1 (MQ=255)
                                                               cGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCG.GGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGAt                         >  2:760514/1‑101 (MQ=255)
                                                               cGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCG.GGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGAt                         <  1:363731/101‑1 (MQ=255)
                                                                gAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCG.GGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATa                        <  1:3753204/101‑1 (MQ=255)
                                                                gAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCG.GGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATa                        >  2:3752929/1‑101 (MQ=255)
                                                                gAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGCG.GGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATa                        <  1:3652253/101‑1 (MQ=255)
                                                                 aaGGTCATGTCGGTCTCCTGAGGGGTTGGGGCG.GGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATag                       >  2:4394152/1‑101 (MQ=255)
                                                                 aaGGTCATGTCGGTCTCCTGAGGGGTTGGGGCA.GGGGTGCC.C.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCAAGAGCGACGCGCCAGCCCGGATag                       >  1:3861696/1‑101 (MQ=255)
                                                                  aGGTCATGTCGGTCTCCTGAGGGGTTGGGGCG.GGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATaga                      <  2:704450/101‑1 (MQ=255)
                                                                  aGGTCATGTCGGTCTCCTGAGGGGTTGGGGCG.GGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATaga                      >  2:3477664/1‑101 (MQ=255)
                                                                      cATGTCGGTCTCCTGAGGGGTTGGGGCG.GGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCtt                  >  2:245798/1‑101 (MQ=255)
                                                                       aTGTCGGTCTCCTGAGGGGTTGGGGCG.GGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTg                 >  2:1603225/1‑101 (MQ=255)
                                                                       aTGTCGGTCTCCTGAGGGGTTGGGGCG.GGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTg                 >  1:4642558/1‑101 (MQ=255)
                                                                          tCGGTCTCCTGAGGGGTTGGGGCG.GGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCg              <  1:32291/101‑1 (MQ=255)
                                                                            ggTCTCCTGAGGGGTTGGGGCG.GGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGc            >  2:2193740/1‑101 (MQ=255)
                                                                               ctcCTGAGGGGTTGGGGCG.GGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATc         <  1:1603330/101‑1 (MQ=255)
                                                                               ctcCTGAGGGGTTGGGGCG.GGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATc         <  2:924143/101‑1 (MQ=255)
                                                                               ctcCTGAGGGGTTGGGGCG.GGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATc         <  2:4442665/101‑1 (MQ=255)
                                                                               ctcCTGAGGGGTTGGGGCG.GGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATc         >  2:4282250/1‑101 (MQ=255)
                                                                               ctcCTGAGGGGTTGGGGCG.GGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATc         <  2:3577261/101‑1 (MQ=255)
                                                                                tcCTGAGGGGTTGGGGCG.GGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCg        >  1:21290/1‑101 (MQ=255)
                                                                                tcCTGAGGGGTTGGGGCG.GGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCg        >  2:1547906/1‑101 (MQ=255)
                                                                                tcCGGAGGGGTTGGGGCG.GGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGTATCg        <  2:1738503/101‑1 (MQ=255)
                                                                                  cTGAGGGGTTGGGGCG.GGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACTCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGtt      <  2:3016360/101‑1 (MQ=255)
                                                                                  cTGAGGGGTTGGGGCG.GGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGtt      >  2:2264117/1‑101 (MQ=255)
                                                                                    gAGGGGTTGGGGCG.GGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCg    >  1:3044598/1‑101 (MQ=255)
                                                                                      ggggTTGGGGCG.GGGGTG.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCggg  <  2:196685/101‑1 (MQ=255)
                                                                                                 |                                                                                             
TTTCACGGCCGAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTT.GGGC..GGGGTG.T.C.CCCCGCCCGGATAGCACCGGCGTTCAGTTAACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGG  >  NC_014034/1974235‑1974419

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG