Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,974,337 +C 100% intergenic (‑30/+25) rpe1 ← / ← cbbM ribulose‑phosphate 3‑epimerase/ribulose bisphosphate carboxylase large subunit

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,974,3371.→C100.0% 731.8 195intergenic (‑30/+25)rpe1/cbbMribulose‑phosphate 3‑epimerase/ribulose bisphosphate carboxylase large subunit
Reads supporting (aligned to +/- strand):  new base (100/94):  ref base (1/0):  total (101/94)

Only 100 of 222 total aligned reads displayed.

GGCCGAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTT..GGGC.GGGGT.G.T.C.CCCCGCCCGGATAGCACCGGCGTTCAGTTAACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGAACGC  >  NC_014034/1974241‑1974433
                                                                                                      |                                                                                                 
ggCCGAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCGGGGGT.G.T.c.c                                                                                                <  1:3258765/101‑1 (MQ=255)
   cGAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCGGGGGG.G.T.c.cccc                                                                                             >  1:4396082/1‑101 (MQ=255)
    gAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCGGGGGT.G.T.c.ccccc                                                                                            <  1:2084156/101‑2 (MQ=255)
       aTCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCGGGGGT.G.G.c.cccccgcc                                                                                         >  2:3631415/1‑97 (MQ=255)
        tCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCGGGGGT.G.T.c.cccccgccc                                                                                        <  1:1428546/101‑6 (MQ=255)
         cGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCGGGGGT.G.TCC.Ccccgcccg                                                                                        <  2:2403548/101‑1 (MQ=255)
         cGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCGGGGGG.G.TCC.Ccccgcccg                                                                                        >  2:3048150/1‑101 (MQ=255)
          ggCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCGGGGGT.G.TCC.CCCCGCCCgg                                                                                       <  2:2544280/101‑1 (MQ=255)
           gCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCGGGGGT.G.TCC.CCCCGCCCGGa                                                                                      >  2:4000568/1‑101 (MQ=255)
             gAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCGGGGGT.G.TCC.CCCCGCCCGGATa                                                                                    >  1:3087462/1‑101 (MQ=255)
             gAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCGGGGGT.G.TCC.CCCCGCCCGGATa                                                                                    >  1:1904428/1‑101 (MQ=255)
              aaGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCGGGGGTGG.C.C.CCCCGCCCGGATAg                                                                                   >  2:1494093/1‑101 (MQ=255)
              aaGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCGGGGGT.G.TCC.CCCCGCCCGGATAg                                                                                   <  2:871860/101‑1 (MQ=255)
               aGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTGGGGGG.GGGGT.G.TCC.CCCCGCCCGGATAGc                                                                                  >  1:493410/1‑101 (MQ=255)
               aGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCGGGGGT.G.TCC.CCCCGCCCGGATAGc                                                                                  >  1:2078582/1‑101 (MQ=255)
               aGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCGGGGGT.G.TCC.CCCCGCCCGGATAGc                                                                                  >  1:3748675/1‑101 (MQ=255)
                gTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCGGGGGT.G.TCC.CCCCGCCCGGATAGCa                                                                                 >  1:4631294/1‑101 (MQ=255)
                 tCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACTGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCGGGGGT.G.TCC.CCCCGCCCGGATAGCAc                                                                                <  1:3856391/101‑1 (MQ=255)
                 tCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCGGGGGT.G.TCC.CCCCGCCCGGATAGCAc                                                                                <  1:1744122/101‑1 (MQ=255)
                  cGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCGGGGGG.G.TCC.CCCCGCCCGGATAGCAcg                                                                               >  2:1512951/1‑100 (MQ=255)
                    gCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGCCGGTCTCCTGGGGGGTTG.GGGC.GGGGG.GCG.CTCCCCGCCCTGATAGCACGgg                                                                             >  2:1087133/1‑101 (MQ=255)
                     cAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCGGGGGT.G.TCC.CCCCGCCCGGATAGCAcggcg                                                                            <  2:3804947/101‑5 (MQ=255)
                        aaaGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCGGGGGT.G.TCC.CCCCGCCCGGATAGCA.CGGCGTTc                                                                        >  1:2478336/1‑101 (MQ=255)
                        aaaGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCGGGGGT.G.TCC.CCCCCCCCGGATAGCA.CGGCGTTc                                                                        >  1:2283156/1‑101 (MQ=255)
                         aaGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCGGGGGT.G.TCC.CCCCGCCCGGATAGCA.CGGCGTTCa                                                                       >  2:2012286/1‑101 (MQ=255)
                         aaGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCGGGGGT.G.TCC.CCCCGCCCGGATAGCA.CGGCGTTCa                                                                       <  2:3721985/101‑1 (MQ=255)
                         aaGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCGGGGGT.G.TCC.CCCCGCCCGGATAGCA.CGGCGTTCa                                                                       <  2:2207072/101‑1 (MQ=255)
                          aGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCGGGGGT.G.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAg                                                                      <  2:4494917/101‑1 (MQ=255)
                          aGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCGGGGGG.G.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAg                                                                      >  1:3016589/1‑101 (MQ=255)
                            gATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCGGGGGT.G.TCC.CCCCGCCCGGATAGGA.CGGCGTTCAGtt                                                                    >  2:869009/1‑101 (MQ=255)
                            gATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCGGGGGT.G.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGtt                                                                    <  1:2909775/101‑1 (MQ=255)
                               cGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCGGGGGT.G.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCAc                                                                 <  1:4333589/101‑1 (MQ=255)
                               cGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCGGGGGT.G.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCAc                                                                 >  1:1354259/1‑101 (MQ=255)
                               cGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCGGGGGT.G.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCAc                                                                 >  1:2216559/1‑101 (MQ=255)
                                gAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCGGGGGT.G.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACg                                                                >  1:260484/1‑101 (MQ=255)
                                gAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCGGGGGT.G.TCC.CCCCGCCCGGATAGCA.CGGCGGTCAGTTCACg                                                                >  2:4317797/1‑101 (MQ=255)
                                gAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCGGGGGG.G.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGGTCACg                                                                >  1:3710824/1‑101 (MQ=255)
                                gAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCGGGGGG.G.TCC.CCCCGCCCGGATAG.AACGGCGTTCAGTTCACg                                                                >  2:3975878/1‑101 (MQ=255)
                                   ggggCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCGGGGGT.G.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGccc                                                             <  2:4362350/101‑1 (MQ=255)
                                    gggCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCGGGGGT.G.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCa                                                            <  2:2487620/101‑1 (MQ=255)
                                     ggCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCGGGGGT.G.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCag                                                           <  1:4070817/101‑1 (MQ=255)
                                      gCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCGGGGGT.G.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCcga                                                          >  1:1000408/1‑101 (MQ=255)
                                      gCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCGGGGGT.G.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCaga                                                          <  2:4472670/101‑1 (MQ=255)
                                      gCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCGGGGGT.G.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCaga                                                          >  2:1903850/1‑101 (MQ=255)
                                       cGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCGGGGGT.G.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCagag                                                         <  2:3497951/101‑1 (MQ=255)
                                        gATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCGGGGGT.G.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGc                                                        <  2:3037499/101‑1 (MQ=255)
                                        gATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCGGGGGT.G.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGc                                                        >  2:1202656/1‑101 (MQ=255)
                                         accTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCGGGGGT.G.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCg                                                       <  1:1087222/99‑1 (MQ=255)
                                          tCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCGGGGGT.G.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCCGAGCGa                                                      >  2:1794570/1‑101 (MQ=255)
                                           cTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCGGGGGC.G.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGAc                                                     >  1:1035408/1‑101 (MQ=255)
                                            ttGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCGGGGGT.G.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGAcg                                                    <  1:4189831/101‑1 (MQ=255)
                                            ttGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCGGGGGT.G.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGAcg                                                    <  2:2681593/101‑1 (MQ=255)
                                             tGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCGGGGGT.T.TGC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGAcgc                                                   >  2:2992314/1‑101 (MQ=255)
                                              gATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCGGGGGT.G.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGAcgcg                                                  <  2:2707430/101‑1 (MQ=255)
                                                tGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCGGGGGT.G.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGcc                                                <  2:4067122/101‑1 (MQ=255)
                                                 gtaaCGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCGGGGGT.G.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCa                                               >  1:703216/3‑101 (MQ=255)
                                                 ggAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCGGGGGT.G.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCa                                               <  2:2161070/101‑1 (MQ=255)
                                                    aCGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCGGGGGT.G.TCC.CCCCCCCCGGATAGCA.CGACGTTCAGTTCACGCCCAGAGCGACGCGCCAGcc                                            >  1:4010499/1‑101 (MQ=255)
                                                     cGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCGGGGGT.G.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGccc                                           <  1:1202748/101‑1 (MQ=255)
                                                         cGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCGGGGGT.G.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGAt                                       <  1:363731/101‑1 (MQ=255)
                                                         cGAAGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCGGGGGT.G.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGAt                                       >  2:760514/1‑101 (MQ=255)
                                                            aGGTCATGTCGGTCTCCTGAGGGGTTG.GGGCGGGGGT.G.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATaga                                    <  2:4401972/101‑1 (MQ=255)
                                                              gTCATGTCGGTCTCCTGAGGGGTTG.GGGCGGGGGT.G.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGATCGACGCGCCAGCCCGGATAGAGc                                  <  2:4122933/101‑1 (MQ=255)
                                                                cATGTCGGTCTCCTGAGGGGTTG.GGGCGGGGGT.G.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCtt                                <  2:703141/101‑1 (MQ=255)
                                                                 aTGTCGGTCTCCTGAGGGGTTG.GGGCGGGGGT.G.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTg                               >  2:1603225/1‑101 (MQ=255)
                                                                 aTGTCGGTCTCCTGAGGGGTTG.GGGCGGGGGG.G.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCCGAGCGACGCGCCCGCCCGGATAGAGCTTg                               >  2:3555791/1‑101 (MQ=255)
                                                                 aTGTCGGTCTCCTGAGGGGTTG.GGGCGGGGGG.G.TCC.CCCCCCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTg                               >  1:581862/1‑101 (MQ=255)
                                                                      ggTCTCCTGAGGGGTTG.GGGCGGGGGT.G.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGc                          >  2:2193740/1‑101 (MQ=255)
                                                                         ctcCTGAGGGGTTG.GGGCGGGGGT.G.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATc                       <  2:4442665/101‑1 (MQ=255)
                                                                         ctcCTGAGGGGTTG.GGGCGGGGGT.G.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATc                       <  1:1603330/101‑1 (MQ=255)
                                                                         ctcCTGAGGGGTTG.GGGCGGGGGT.G.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATc                       <  1:3434352/101‑1 (MQ=255)
                                                                          tcCTGAGGGGTTG.GGGCGGGGGT.G.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCg                      >  2:1547906/1‑101 (MQ=255)
                                                                          tcCTGAGGGGTTG.GGGCGGGGGT.G.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCg                      >  1:21290/1‑101 (MQ=255)
                                                                          tcCGGAGGGGTTG.GGGCGGGGGT.G.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGTATCg                      <  2:1738503/101‑1 (MQ=255)
                                                                           ccTGAGGGGTTG.GGGCGGGGGT.G.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGt                     <  1:1278352/101‑1 (MQ=255)
                                                                            cTGAGGGGTTG.GGGCGGGGGT.G.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACTCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGtt                    <  2:3016360/101‑1 (MQ=255)
                                                                             tGAGGGGTTG.GGGCGGGGGT.G.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTc                   <  1:2992543/101‑1 (MQ=255)
                                                                              gAGGGGTTG.GGGCGGGGGT.G.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCg                  >  2:3132383/1‑101 (MQ=255)
                                                                              gAGGGGTTG.GGGCGGGGGT.G.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCg                  >  1:3044598/1‑101 (MQ=255)
                                                                                    gttggggcgggggT.G.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAg             <  2:1941120/92‑1 (MQ=255)
                                                                                      tggggcgggggT.G.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCt           >  1:1465107/8‑101 (MQ=255)
                                                                                      tggggcgggggT.G.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCt           >  2:1148186/8‑101 (MQ=255)
                                                                                      tggggcgggggT.G.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCt           >  1:773143/8‑101 (MQ=255)
                                                                                      tggggcgggggT.G.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCt           >  2:238057/8‑101 (MQ=255)
                                                                                      tggggcgggggT.G.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCt           >  2:2327528/8‑101 (MQ=255)
                                                                                       ggggcgggggT.G.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCtt          >  1:2805229/7‑101 (MQ=255)
                                                                                       ggggcgggggT.G.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCtt          >  2:830257/7‑101 (MQ=255)
                                                                                       ggggcgggggT.G.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCtt          <  2:2667459/95‑1 (MQ=255)
                                                                                        gggcgggggT.G.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCttt         <  2:2235231/96‑1 (MQ=255)
                                                                                         ggcgggggT.G.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTc        <  2:4571782/97‑1 (MQ=255)
                                                                                         ggcgggggT.G.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTc        >  2:1125482/5‑101 (MQ=255)
                                                                                          gcgggggT.G.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCGGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCg       <  1:1574231/98‑1 (MQ=255)
                                                                                          gcgggggT.G.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCg       <  1:3885307/98‑1 (MQ=255)
                                                                                           ggggggT.G.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGa      <  2:282659/99‑1 (MQ=255)
                                                                                           cgggggT.G.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGa      <  1:1705629/99‑1 (MQ=255)
                                                                                           cgggggT.G.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGa      >  2:1654258/3‑101 (MQ=255)
                                                                                            gggggT.G.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGaa     >  1:198600/2‑101 (MQ=255)
                                                                                            gggggT.G.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGaa     >  1:3628264/2‑101 (MQ=255)
                                                                                            gggggT.G.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGaa     <  1:1513060/100‑1 (MQ=255)
                                                                                              gggT.G.TCC.CCCCGCCCGGATAGCA.CGGCGTTCAGTTCACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGAACg   >  2:3885860/1‑101 (MQ=255)
                                                                                                      |                                                                                                 
GGCCGAAATCGGCGAAGTCGGCAGAAAGGATCGAGGGGGCGATCTTGATGGAACGGTCGAAGGTCATGTCGGTCTCCTGAGGGGTT..GGGC.GGGGT.G.T.C.CCCCGCCCGGATAGCACCGGCGTTCAGTTAACGCCCAGAGCGACGCGCCAGCCCGGATAGAGCTTGTCGGCATCGTTCGGGAAGCTTTCGAACGC  >  NC_014034/1974241‑1974433

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG