BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,997,981	+G	95.5%	intergenic (‑46/‑119)	RCAP_rcc01844 ← / → RCAP_rcc01845	sensor histidine kinase/hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,997,981	1	.→G	95.5%	22.3 ‑1.6 ‑0.9	220	intergenic (‑46/‑119)	RCAP_rcc01844/RCAP_rcc01845	sensor histidine kinase/hypothetical protein
Reads supporting (aligned to +/- strand): new base (111/99): ref base (3/7): total (114/106)
Fisher's exact test strand distribution p-value = 2.02e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.27e-01

Only 100 of 234 total aligned reads displayed.


GCAGAATCGGAAACGGTAACGTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTT.C.GG.GAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGATGTTGTTGTCATGAGCGATGGGTTT  >  NC_014034/1997881‑1998076


gCAGAATCGGAAACGGTAACGTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTT.c                                                                                                   <  2:4571631/101‑1 (MQ=255)


gCAGAATCGGAAACGGTAACGTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTT.c                                                                                                   <  2:2707419/101‑1 (MQ=255)


    aaTCGGAAACGGTAACGTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTT.C.gg.gg                                                                                             >  1:249354/1‑100 (MQ=255)


     aTCGGAAACGGTAACGTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTT.C.gg.gga                                                                                            <  2:303412/101‑3 (MQ=255)


        ggAAACGGTAACGTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTT.C.GG.Ggaaac                                                                                         >  2:2287093/1‑99 (MQ=255)


         gAAACGGTAACGTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTT.CGGG.GAAACg                                                                                         <  2:3004548/101‑1 (MQ=255)


         gAAACGGTAACGTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTT.CGGG.GAAACg                                                                                         <  2:2716356/101‑1 (MQ=255)


         gAAACGGTAACGTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTT.CGGG.GAAACg                                                                                         <  1:749336/101‑1 (MQ=255)


          aaaCGGTAACGTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTT.CGGG.GAAACGa                                                                                        >  1:3300964/1‑101 (MQ=255)


            aCGGTAACGTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTT.CGGG.GAAACGATa                                                                                      >  2:2025643/1‑101 (MQ=255)


            aCGGTAACGTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTT.C.GG.GGAAAGagtg                                                                                     <  2:3815847/101‑4 (MQ=255)


             cGGTAACGTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTT.CGGG.GAAACGATAc                                                                                     >  2:3340723/1‑101 (MQ=255)


              ggTAACGTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTT.CGGG.GAAACGATAcc                                                                                    >  2:3397700/1‑101 (MQ=255)


                tAACGTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTT.CGGG.GAAACGATACCCa                                                                                  <  1:559091/101‑1 (MQ=255)


                 aaCGTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTT.CGGG.GAAACGATACCCaa                                                                                 >  2:536567/1‑101 (MQ=255)


                   cGTCTCGGCCAAGGAGATTGTTGACGCATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTT.CGGG.GAAACGATACCCAAGt                                                                               <  1:2804217/101‑1 (MQ=255)


                   cGTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTT.CGGG.GAAACGATACCCAAGt                                                                               <  1:4499664/101‑1 (MQ=255)


                    gTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTT.CGGG.GAAACGATACCCAAGtt                                                                              >  1:2560432/1‑101 (MQ=255)


                      ctcGGCGAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTT.CGGG.GAAACGATACCCAAGTTCa                                                                            <  2:3583853/101‑1 (MQ=255)


                      ctcGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTT.CGGG.GAAACGATACCCAAGTTCa                                                                            <  2:4104255/101‑1 (MQ=255)


                      ctcGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTT.CGGG.GAAACGATACCCAAGTTCa                                                                            >  2:248180/1‑101 (MQ=255)


                       tcGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTT.CGGG.GAAACGATACCCAAGTTCaa                                                                           >  2:2755712/1‑101 (MQ=255)


                       tcGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTT.CGGG.GAAACGATACCCAAGTTCaa                                                                           <  1:4653216/101‑1 (MQ=255)


                          gCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTT.CGGG.GAAACGATACCCAAGTTCAAGGc                                                                        >  1:3763257/1‑101 (MQ=255)


                          gCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTT.CGGG.GAAACGATACCCAAGTTCAAGGc                                                                        >  2:2348425/1‑101 (MQ=255)


                            cAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTT.CGGG.GAAACGATACCCAAGTTCAAGGCCg                                                                      <  2:3171636/101‑1 (MQ=255)


                             aaGGAGATTGTTGACGAATCCGCCATTTTTTGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTTAG.GG.GAATCGATGCCCAAGTTCAAGGCGGc                                                                     >  2:773467/1‑101 (MQ=255)


                             aaGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTT.CGGG.GAAACGATACCCAAGTTCAAGGCCGc                                                                     >  2:3653195/1‑101 (MQ=255)


                              aGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTT.CGGG.GAAACGATACCCAAGTTCAAGGCCGCa                                                                    >  2:2190783/1‑101 (MQ=255)


                               ggAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTT.CGGG.GAAACGATACCCAAGTTCAAGGCCGCAt                                                                   >  2:884401/1‑101 (MQ=255)


                               ggAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTT.CGGG.GAAACGATACCCAAGTTCAAGGCCGCAt                                                                   <  1:3013420/101‑1 (MQ=255)


                               ggAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTT.CGGG.GAAACGATACCCAAGTTCAAGGCCGCAt                                                                   <  2:40070/101‑1 (MQ=255)


                               ggAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTT.CGGG.GAAACGATACCCAAGTTCAAGGCCGCAt                                                                   <  1:2245236/101‑1 (MQ=255)


                                 agaTTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTT.CGGG.GAAACGATACCCAAGTTCAAGGCCGCATGc                                                                 >  2:243866/1‑101 (MQ=255)


                                  gaTTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTT.CGGG.GAAACGATACCCAAGTTCAAGGCCGCATGCa                                                                <  1:42731/101‑1 (MQ=255)


                                   nttntngACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTT.CGGG.GAAACGATACCCAAGTTCAAGGCCGCATGCan                                                               <  1:959847/100‑2 (MQ=255)


                                   aTTGTTGGCGAATCCGCCATTTTTCGGTCCCCCTGACATATCCAATGCTTGACCCGAGCAAGCTT.CGGG.GAAACGATACCCAAGTTCAAGGCCGCATGCaa                                                               <  1:3569100/101‑1 (MQ=255)


                                   aTTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTT.CGGG.GAAACGATACCCAAGTTCAAGGCCGCATGCaa                                                               >  2:4383846/1‑101 (MQ=255)


                                     tgttgACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTT.CGGG.GAAACGATACCCAAGTTCAAGGCCGCATGCaaaa                                                             >  2:450980/1‑101 (MQ=255)


                                     tgttgACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTT.CGGG.GAAACGATACCCAAGTTCAAGGCCGCATGCaaaa                                                             <  2:4449816/101‑1 (MQ=255)


                                      gttgACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTT.CGGG.GAAACGATACCCAAGTTCAAGGCCGCATGCAAAAg                                                            <  2:589860/101‑1 (MQ=255)


                                        tgACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTT.CGGG.GAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGcc                                                          >  1:4238140/1‑101 (MQ=255)


                                        tgACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTT.CGGG.GAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGcc                                                          <  2:3714521/101‑1 (MQ=255)


                                        tgACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTT.CGGG.GAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGcc                                                          >  2:2586771/1‑101 (MQ=255)


                                        tgACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTT.CGGG.GAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGcc                                                          <  1:1157243/101‑1 (MQ=255)


                                         gACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGCCCCGAGCAAGCTT.CGGG.GAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCa                                                         <  1:2025783/101‑1 (MQ=255)


                                         gACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTT.CGGG.GAAATGATACCCAAGTTCAAGGCCGCATGCAAAAGCCa                                                         >  1:4009721/1‑101 (MQ=255)


                                         gACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTT.CGGG.GAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCa                                                         >  1:2172186/1‑101 (MQ=255)


                                          aCGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTT.CGGG.GAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAg                                                        >  1:3941948/1‑101 (MQ=255)


                                          aCGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTT.CGGG.GAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAg                                                        <  2:3771434/101‑1 (MQ=255)


                                          aCGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTT.CGGG.GAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAg                                                        <  2:1120103/101‑1 (MQ=255)


                                          aCGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTT.CGGG.GAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAg                                                        <  1:1587120/101‑1 (MQ=255)


                                           cGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTT.CGGG.GAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAgg                                                       >  1:3188963/1‑101 (MQ=255)


                                             aaTCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTT.CGGG.GAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAgggg                                                     >  1:2463971/1‑101 (MQ=255)


                                              aTCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTT.CGGG.GAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAggggg                                                    <  1:454608/101‑1 (MQ=255)


                                              aTCCGCCATTTTTCGCTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTT.CGGG.GAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAggggg                                                    <  2:141463/101‑1 (MQ=255)


                                                ccGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTT.CGGG.GAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGc                                                  <  2:1289727/101‑1 (MQ=255)


                                                 cGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTT.CGGG.GAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCt                                                 <  2:3300719/101‑1 (MQ=255)


                                                  gcaaTTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTT.CGGG.GAAAAGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTa                                                >  2:1879142/4‑101 (MQ=255)


                                                   ccttttttCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTT.CGGG.GAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTaa                                               <  2:3934314/98‑1 (MQ=255)


                                                    cATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTT.CGGG.GAAACGATACCCAGGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAg                                              <  1:1870231/101‑1 (MQ=255)


                                                     aTTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTT.CGGG.GAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGc                                             <  1:2932615/101‑1 (MQ=255)


                                                     aTTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTT.CGGG.GAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGc                                             <  1:915558/101‑1 (MQ=255)


                                                      tttttCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTT.CGGG.GAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCt                                            >  1:1710355/1‑101 (MQ=255)


                                                       ttttCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTT.CGGG.GAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCtt                                           <  2:659464/101‑1 (MQ=255)


                                                       ttttCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTT.CGGG.GAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCtt                                           >  1:1492923/1‑101 (MQ=255)


                                                         ttCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTT.CGGG.GAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCtttt                                         <  1:1030042/101‑1 (MQ=255)


                                                          tCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTT.CGGG.GAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTg                                        >  1:3403274/1‑101 (MQ=255)


                                                           cGGTCCCCCTGACATATCCAATGCTTGACCCGAGCAAGCTT.CGGG.GAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGc                                       <  2:1831697/101‑1 (MQ=255)


                                                            ggTCCCCCTGACATATCCAATGCTTGACCCGAGCAAGCTT.CGGG.GAAACGATACCCAAGTTCAAGGCCGGATGCAAAATCCAGGGGGGCTAAGCTTTTGCt                                      <  1:4205477/101‑1 (MQ=255)


                                                               cACCCTGACATATCCAATGCTTGACCCGAGCAAGCTT.CGGG.GAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGaa                                   >  1:2697474/1‑101 (MQ=255)


                                                               cACCCTGACATATCCAATGCTTGACCCGAGCAAGCTT.CGGG.GAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGaa                                   >  2:3141789/1‑101 (MQ=255)


                                                                  ccTGACATATCCAATGCTTGACCCGAGCAAGCTT.CGGG.GAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTc                                <  1:62096/101‑1 (MQ=255)


                                                                    tGACATATCCAATGCTTGACCCGAGCAAGCTT.CGGG.GAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTcgc                              <  1:3035207/101‑1 (MQ=255)


                                                                      aCATATCCAATGCTTGACCCGAGCAAGCTT.CGGG.GAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCgg                            >  2:2986929/1‑101 (MQ=255)


                                                                      aCATATCCAATGCTTGACCCGAGCAAGCTT.CGGG.GAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCgg                            >  2:3858003/1‑101 (MQ=255)


                                                                        atatCCAATGCTTGACCCGAGCAAGCTT.CGGG.GAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGAt                          >  1:379304/1‑101 (MQ=255)


                                                                        atatCCAATGCTTGACCCGAGCAAGCTT.CGGG.GAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGAt                          >  1:1081969/1‑101 (MQ=255)


                                                                        atatCCAATGCTTGACCCGAGCAAGCTT.CGGG.GAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGAt                          >  1:1991128/1‑101 (MQ=255)


                                                                          atCCAATGCTTGACCCGAGCAAGCTT.CGGG.GAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGAtgt                        <  1:3190843/101‑1 (MQ=255)


                                                                          atCCAATGCTTGACCCGAGCAAGCTT.CGGG.GAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGAtgt                        >  1:1276958/1‑101 (MQ=255)


                                                                           tCCAATGCTTGACCCGAGCAAGCTT.CGGG.GAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGAtgtt                       <  1:3817401/101‑1 (MQ=255)


                                                                            ccAATGCTTGACCCGAGCAAGCTT.CGGG.GAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGAtgttg                      >  2:1608304/1‑101 (MQ=255)


                                                                             cAATGCTTGACCCGAGCAAGCTT.C.GGTGAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGAtgttgt                     <  1:1550169/101‑1 (MQ=255)


                                                                               aTGCTTGACCCGAGCAAGCTT.CGGG.GAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGAtgttgttg                   >  1:4450358/1‑101 (MQ=255)


                                                                                tGCTTGACCCGAGCAAGCTT.CGGG.GAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGAtgttgttgc                  >  2:1308144/1‑100 (MQ=255)


                                                                                 gCTTGACCCGAGCAAGCTT.CGGG.GAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGATGTTGTTGTc                 >  1:1762567/1‑101 (MQ=255)


                                                                                 gCTTGACCCGAGCAAGCTT.CGGG.GAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGATGTTGTTGTc                 <  1:2068647/101‑1 (MQ=255)


                                                                                   ttGACCCGAGCAAGCTT.CGGG.GAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGATGTTGTTGTCAt               >  1:1815993/1‑101 (MQ=255)


                                                                                     gACCCGAGCAAGCTT.CGGG.GAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGATGTTGTTGCCATGa             <  1:2348587/101‑1 (MQ=255)


                                                                                      aCCCGAGCAAGCTT.CGGG.GAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGATGTTGTTGTCATGAg            >  1:404894/1‑101 (MQ=255)


                                                                                       cccGAGCAAGCTT.CGGG.GAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGATGTTGTTGTCATGAGc           <  2:4237852/101‑1 (MQ=255)


                                                                                       cccGAGCAAGCTT.CGGG.GAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGATGTTGTTGTCATGAGc           <  1:2288863/101‑1 (MQ=255)


                                                                                       cccGAGCAAGCTT.CGGG.GAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGATGTTGTTGTCATGAGc           <  1:3144020/101‑1 (MQ=255)


                                                                                       cccGAGCAAGCTT.CGGG.GAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGACAGGGGGGCTAAGCTTTTGCTGAATTCGCGGATGTTGTTGTCATGAGc           <  1:773542/101‑1 (MQ=255)


                                                                                        ccGAGCAAGCTT.CGGG.GAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGATGTTGTTGTCATGAGCg          >  2:4054231/1‑101 (MQ=255)


                                                                                            gCAAGCTT.CGGG.GAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGATGTTGTTGTCATGAGCGATgg      <  2:1492814/101‑1 (MQ=255)


                                                                                              aaGCTT.CGGG.GAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGATGTTGTTGTCATGAGCGATGGGt    >  2:3225429/1‑101 (MQ=255)


                                                                                                gCTT.CGGG.GAAACGATACCCAAGTTCAAGGCCGGATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGATGTTGTTGTCATGAGCGATGGGttt  >  2:1195939/1‑101 (MQ=255)


                                                                                                gCTT.CGGG.GAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGCATTCGCGGATGTTGTTGTCATGAGCGATGGGttt  >  1:133823/1‑101 (MQ=255)


GCAGAATCGGAAACGGTAACGTCTCGGCCAAGGAGATTGTTGACGAATCCGCCATTTTTCGGTCACCCTGACATATCCAATGCTTGACCCGAGCAAGCTT.C.GG.GAAACGATACCCAAGTTCAAGGCCGCATGCAAAAGCCAGGGGGGCTAAGCTTTTGCTGAATTCGCGGATGTTGTTGTCATGAGCGATGGGTTT  >  NC_014034/1997881‑1998076

Base quality scores:


ATCG

< 3 ≤


ATCG

< 24 ≤


ATCG

< 31 ≤


ATCG

< 35 ≤


ATCG

< 41 ≤


ATCG