BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,047,090	+C	88.5%	coding (20/879 nt)	potH1 ←	polyamine ABC transporter permease PotH

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,047,090	1	.→C	88.5%	72.8 ‑0.2 ‑1.2	209	coding (20/879 nt)	potH1	polyamine ABC transporter permease PotH
Reads supporting (aligned to +/- strand): new base (86/99): ref base (12/12): total (98/111)
Fisher's exact test strand distribution p-value = 8.29e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.73e-02

Only 100 of 225 total aligned reads displayed.


CGACGATGGTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCG.CCACCG.CGGG.TGGAGATGCCGACATACTCTATTCT.CCTCAGCG.C.TCTCA.GCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGG  >  NC_014034/2046992‑2047178


cgacgaTGGTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCTAGCGCGCCCACcg.c                                                                                              <  2:3909768/101‑1 (MQ=255)


cgacgaTGGTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACcg.c                                                                                              >  2:2408817/1‑101 (MQ=255)


cgacgaTGGTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACcg.c                                                                                              >  2:1232727/1‑101 (MQ=255)


 gacgaTGGTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACcg.cc                                                                                             >  2:4444652/1‑100 (MQ=255)


 gacgaTGGTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACcg.cc                                                                                             >  2:2305955/1‑100 (MQ=255)


 gacgaTGGTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACcg.cc                                                                                             <  2:523541/101‑2 (MQ=255)


 gacgaTGGTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACcg.cc                                                                                             >  2:2918896/1‑100 (MQ=255)


 gacgaTGGTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACcg.cc                                                                                             >  1:1161546/1‑100 (MQ=255)


 gacgaTGGTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACcg.cc                                                                                             <  2:4014287/101‑2 (MQ=255)


  acgaTGGTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACcg.ccg                                                                                            <  1:1821919/101‑3 (MQ=255)


  acgaTGGTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACcg.ccg                                                                                            >  1:1096227/1‑99 (MQ=255)


   cgaTGGTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCG.Ccgg                                                                                           >  1:774363/1‑101 (MQ=255)


    gaTGGTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCG.Ccggg                                                                                          <  2:1480986/101‑2 (MQ=255)


     aTGGTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCG.Ccgggt                                                                                         <  2:2725085/101‑3 (MQ=255)


     aTGGTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCG.Ccgggt                                                                                         >  1:1753834/1‑99 (MQ=255)


      tGGTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCG.Ccgggtt                                                                                        >  1:1417464/1‑98 (MQ=255)


      tGGTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCAGCTGGCCGCCTCCGAGCGCGCCCACCG.Ccgggtt                                                                                        >  1:4003911/1‑98 (MQ=255)


       ggTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCG.Ccgggttg                                                                                       >  1:2624165/1‑97 (MQ=255)


        gTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCG.Ccgggttgg                                                                                      <  2:4408399/101‑6 (MQ=255)


         ccACCAGCGGGCCCGCCAGCAACAGAACTGCATAGATCGGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCG.Ccgggttgga                                                                                     <  1:2125639/100‑7 (MQ=255)


          cACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCG.Ccgggttggag                                                                                    >  1:3002725/1‑94 (MQ=255)


          cACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCG.Ccgggttggag                                                                                    <  1:1011780/101‑8 (MQ=255)


           aCCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCG.Ccgggttggaga                                                                                   <  1:1919405/101‑9 (MQ=255)


           aCCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCG.Ccgggtagatcg                                                                                   >  1:1661136/1‑93 (MQ=255)


            ccAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGAt                                                                                   <  2:4609649/101‑1 (MQ=255)


            ccAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGAt                                                                                   <  1:2324111/101‑1 (MQ=255)


             cAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATg                                                                                  <  2:439965/101‑1 (MQ=255)


              aGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGc                                                                                 >  1:2582972/1‑101 (MQ=255)


              aGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGc                                                                                 >  2:2048329/1‑101 (MQ=255)


                gggggCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCg                                                                               <  1:1637657/100‑1 (MQ=255)


                cGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCg                                                                               <  1:4096627/101‑1 (MQ=255)


                 ggggCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGa                                                                              >  1:2220827/1‑101 (MQ=255)


                  gggCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGAc                                                                             <  2:1096138/101‑1 (MQ=255)


                   ggCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACa                                                                            >  1:2307900/1‑101 (MQ=255)


                    gccgccAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACAt                                                                           <  2:4136321/101‑1 (MQ=255)


                    gccgccAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACAt                                                                           >  2:3375997/1‑101 (MQ=255)


                      cgccAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATAc                                                                         >  2:2491276/1‑101 (MQ=255)


                      cgccAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATAc                                                                         >  2:4437173/1‑101 (MQ=255)


                       gccAGCAACAGAACTGCATAGATCAGCGTTGGGCTGCCCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATAct                                                                        <  2:387166/101‑1 (MQ=255)


                        ccAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATActc                                                                       <  2:2976215/101‑1 (MQ=255)


                        ccAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATActc                                                                       <  1:1239851/101‑1 (MQ=255)


                        ccAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATActc                                                                       <  1:1567278/101‑1 (MQ=255)


                         cAGCAACAGACCTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATActct                                                                      <  2:1363151/101‑1 (MQ=255)


                         cAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATActct                                                                      >  2:2573025/1‑101 (MQ=255)


                             aaCAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATtc                                                                  <  1:4168656/101‑1 (MQ=255)


                              aCAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATtct                                                                 <  2:2592932/101‑1 (MQ=255)


                              aCAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATtct                                                                 >  1:3577/1‑101 (MQ=255)


                                aGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGCTGCCGACATACCATATCct.cc                                                              >  2:3403124/1‑101 (MQ=255)


                                  aaCTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTct.ccct                                                            >  1:3108256/1‑99 (MQ=255)


                                    cTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTct.ccctca                                                          <  1:601595/101‑5 (MQ=255)


                                     tGCATAGATCAGCGTTGGGCTGACCATGACCCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTct.ccctcag                                                         <  1:3038217/101‑6 (MQ=255)


                                      gCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAgc                                                         >  1:4360928/1‑101 (MQ=255)


                                       cATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAgcg                                                        >  1:2810185/1‑101 (MQ=255)


                                          aGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAgcg.cgt                                                    <  2:2823458/101‑3 (MQ=255)


                                             tCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTGCCTCAgcg.cgtctc                                                 >  2:3080117/1‑96 (MQ=255)


                                               aGCGTTGGGCTGACCATGACCCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCG.CGTCTCA.g                                              <  2:1624823/101‑1 (MQ=255)


                                               aGCGTTGGGCTGACCATGACCCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCG.CGTCTCA.g                                              <  2:2220665/101‑1 (MQ=255)


                                                 cGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCG.CGTCTCA.ggg                                            >  2:2538295/1‑99 (MQ=255)


                                                 cGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCG.CGTCTCA.ggc                                            >  2:4250759/1‑99 (MQ=255)


                                                    tGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTTTCCCTCAGCG.CGTCTCA.ggcgcg                                         <  1:3750868/101‑6 (MQ=255)


                                                       gCTGACAATGACCCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCG.CGTCTCAGGCGCGttt                                       <  2:1277609/101‑1 (MQ=255)


                                                          gACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCG.CGTCTCAGGCGCGTTTggg                                    >  1:2469465/1‑101 (MQ=255)


                                                               tGACCCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCG.CGTCTCAGGCGCGTTTGGGCCaaa                               <  1:2178057/101‑1 (MQ=255)


                                                               tGAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCG.CGTCTCAGGCGCGTTTGGGCCaaa                               >  2:2673682/1‑101 (MQ=255)


                                                                gAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCG.CGTCTCAGGCGCGTTTGGGCCAAAt                              >  1:4624198/1‑101 (MQ=255)


                                                                gAACCAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCG.CGTCTCAGGCGCGTTTGGGCCAAAt                              >  1:396303/1‑101 (MQ=255)


                                                                  aCCAGCCGCTGGCCGCCTCCGAGCGCGCCCCCCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCG.CGTCTCAGGCGCGTTTGGGCCAAAttt                            <  1:4232671/101‑1 (MQ=255)


                                                                    cAGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCG.CGTCTCAGGCGCGTTTGGGCCAAATTTGc                          >  2:2525032/1‑101 (MQ=255)


                                                                     aGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCG.CGTCTCAGGCGCGTTTGGGCCAAATTTGca                         <  2:1982950/101‑1 (MQ=255)


                                                                     aGCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCG.CGTCTCAGGCGCGTTTGGGCCAAATTTGca                         >  2:18646/1‑101 (MQ=255)


                                                                      gCCGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCGTG.TCTCAGGCGCGTTTGGGCCAAATTTGcac                        <  1:4151676/101‑1 (MQ=255)


                                                                       ccGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCG.CGTCTCAGGCGCGTTTGGGCCAAATTTGcaca                       <  2:1743005/101‑1 (MQ=255)


                                                                       ccGCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCG.CGTCTCAGGCGCGTTTGGGCCAAATTTGcaca                       <  2:396266/101‑1 (MQ=255)


                                                                         gCTGGCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCG.CGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAg                     <  1:4437468/101‑1 (MQ=255)


                                                                            ggCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCG.CGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGtt                  >  1:1984653/1‑101 (MQ=255)


                                                                            ggCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCG.CGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGtt                  >  1:4568579/1‑101 (MQ=255)


                                                                            ggCCGCCTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCG.CGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGtt                  <  2:285606/101‑1 (MQ=255)


                                                                             gccgccTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCG.CGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGttt                 <  2:774288/101‑1 (MQ=255)


                                                                              ccgccTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCG.CGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTc                <  1:1034537/101‑1 (MQ=255)


                                                                              ccgccTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCG.CGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTc                <  1:662887/101‑1 (MQ=255)


                                                                                gccTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCG.CGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCgg              >  2:611983/1‑101 (MQ=255)


                                                                                gccTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCG.CGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCgg              >  2:344110/1‑101 (MQ=255)


                                                                                gccTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCG.CGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCgg              >  2:1003351/1‑101 (MQ=255)


                                                                                gccTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCG.CGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCgg              >  1:2447431/1‑101 (MQ=255)


                                                                                  cTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCG.CGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCa            <  2:3238858/101‑1 (MQ=255)


                                                                                  cTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCG.CGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCa            <  1:842432/101‑1 (MQ=255)


                                                                                  cTCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCG.CGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCa            <  1:3897464/101‑1 (MQ=255)


                                                                                   tCCGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCG.CGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCag           >  1:52611/1‑101 (MQ=255)


                                                                                    ccGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCG.CGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCaga          >  1:3307687/1‑101 (MQ=255)


                                                                                     cGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCG.CGTCTCAGGCGCGTTTGGGCCAACTTTGCACAAGGTTTCGGCagag         >  1:4192875/1‑101 (MQ=255)


                                                                                     cGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCG.CGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCagag         >  2:234983/1‑101 (MQ=255)


                                                                                     cGAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCG.CGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCagag         >  1:4037622/1‑101 (MQ=255)


                                                                                      gAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCG.CGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAgg        <  1:2077358/101‑1 (MQ=255)


                                                                                      gAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCG.CGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAgg        >  2:4260688/1‑101 (MQ=255)


                                                                                      gAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCG.CGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAgg        >  2:4178009/1‑101 (MQ=255)


                                                                                      gAGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCG.CGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAgg        <  2:2623980/101‑1 (MQ=255)


                                                                                       aGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCG.CGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGa       <  1:2379565/101‑1 (MQ=255)


                                                                                       aGCGCGCCCACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCG.CGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGa       <  2:166456/101‑1 (MQ=255)


                                                                                         cgcgcccACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCG.CGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGaaa     <  1:578000/96‑1 (MQ=255)


                                                                                         cgcgcccACCGCCGGGTTGGAGATGCCGACATACTCTATTCTCCCTCAGCG.CGTCTCAGGCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGaaa     >  2:3667098/6‑101 (MQ=255)


CGACGATGGTCACCAGCGGGGCCGCCAGCAACAGAACTGCATAGATCAGCGTTGGGCTGACCATGAACCAGCCGCTGGCCGCCTCCGAGCGCG.CCACCG.CGGG.TGGAGATGCCGACATACTCTATTCT.CCTCAGCG.C.TCTCA.GCGCGTTTGGGCCAAATTTGCACAAGGTTTCGGCAGAGGAAAGGG  >  NC_014034/2046992‑2047178

Base quality scores:


ATCG

< 3 ≤


ATCG

< 20 ≤


ATCG

< 29 ≤


ATCG

< 34 ≤


ATCG

< 41 ≤


ATCG