Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,057,830 +C 86.2% intergenic (‑596/‑306) RCAP_rcc01900 ← / → RCAP_rcc01901 hemolysin‑type calcium‑binding repeat family protein/polyphosphate kinase 2 domain‑containing protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,057,8301.→C86.2% 73.5 ‑1.3 ‑0.2174intergenic (‑596/‑306)RCAP_rcc01900/RCAP_rcc01901hemolysin‑type calcium‑binding repeat family protein/polyphosphate kinase 2 domain‑containing protein
Reads supporting (aligned to +/- strand):  new base (67/83):  ref base (14/10):  total (81/93)
Fisher's exact test strand distribution p-value = 2.71e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.24e-01

Only 100 of 187 total aligned reads displayed.

GTCCTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.CC.GCG.CGC.AGC.G.TTCCGAACCGGCATCAATCTTG.G.CCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCAAAGC  >  NC_014034/2057732‑2057913
                                                                                                      |                                                                                        
 tCCTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.Cc..cg.cgcc                                                                                      >  2:313360/1‑100 (MQ=255)
   cTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACCA.T.CC..CG.CGC.CGc                                                                                   >  2:3568007/1‑101 (MQ=255)
    tGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.CC..CG.CGC.CGca                                                                                  >  1:931340/1‑100 (MQ=255)
     gTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.CC..CG.CGC.CGcca                                                                                 >  2:3247834/1‑99 (MQ=255)
     gTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.CC..CG.CGC.CGcaa                                                                                 >  1:3479686/1‑99 (MQ=255)
     gTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGAGTGGCGACAA.T.CC..CG.CGC.CGcaa                                                                                 >  2:775781/1‑99 (MQ=255)
      tttACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.CC..CG.CGC.CGcaag                                                                                >  2:1547553/1‑98 (MQ=255)
      tttACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.CC..CG.CGC.CGcaag                                                                                <  2:118209/101‑4 (MQ=255)
      tttACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.CC..CG.CGC.CGcaag                                                                                <  2:2498980/101‑4 (MQ=255)
         aCCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.CC..CG.CGC.CGcaagcgt                                                                             >  2:4586831/1‑95 (MQ=255)
         aCCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.CC..CG.CGC.CGcaagcgt                                                                             >  1:3974896/1‑95 (MQ=255)
         aCCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.CC..CG.CGC.CGcaagcgt                                                                             >  1:4069879/1‑95 (MQ=255)
          ccGCCCATTCACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.CC..CG.CGC.CGcaagcgtt                                                                            <  2:1511619/101‑8 (MQ=255)
                   aaCCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCC.GCGCCGCAAGC.GTTTCCGAACCg                                                                      >  2:2732235/1‑101 (MQ=255)
                    aCCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCC.GCGCCGCAAGC.GTTTCCGAACCgg                                                                     <  1:3510572/101‑1 (MQ=255)
                     cccTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCC.GCGCCGCAAGC.GTTTCCGAACCGGc                                                                    <  1:172233/101‑1 (MQ=255)
                     cccTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCC.GCGCCGCAAGC.GTTTCCGAACCGGc                                                                    <  2:104644/101‑1 (MQ=255)
                      ccTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCC.GCGCCGCAAGC.GTTTCCGAACCGGCa                                                                   <  1:799349/101‑1 (MQ=255)
                      ccTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCC.GCGCCGCAAGC.GTTTCCGAACCGGCa                                                                   <  2:2111029/101‑1 (MQ=255)
                       cTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCC.GCGCCGCAAGC.GTTTCCGAACCGGCAt                                                                  <  1:3801487/101‑1 (MQ=255)
                          cTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCTACAA.TCCC.GCGCCGCAAGC.GTTTCCGAACCGGCATCat                                                               >  1:1507914/1‑100 (MQ=255)
                          cTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCC.GCGCCGCAAGC.GTTTCCGAACCGGGATCat                                                               >  2:4301480/1‑100 (MQ=255)
                          cTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCCGCGTTTCCGGATTGGCGTCAA.TCCC.GCGCCGCAAGC.GTTTCCGAACCGGCATCat                                                               <  1:1425074/101‑2 (MQ=255)
                           ttCGGCGCGCGGACTTCGGGGTGGTGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCC.GCGCCGCAAGC.GTTTCCGAACCGGCATCatt                                                              <  1:2899097/101‑3 (MQ=255)
                           ttCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCC.GCGCCGCAAGC.GTTTCCGAACCGGCATCatt                                                              <  1:3178504/101‑3 (MQ=255)
                           ttCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCCGCGTTTCCGGATTGGCGACAA.TCCC.GCGCCGCAAGC.GTTTCCGAACCGGCATCatt                                                              <  1:2364552/101‑3 (MQ=255)
                             cGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCC.GCGCCGCAAGC.GTTTCCGAACCGGCATCATTCt                                                            <  1:1559179/101‑1 (MQ=255)
                             cGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCC.GCGCCGCAAGC.GTTTCCGAACCGGCATCATTCt                                                            >  1:3731874/1‑101 (MQ=255)
                              ggCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCC.GCGCCGCAAGC.GTTTCCGAACCGGCATCATTCtt                                                           <  2:4403140/101‑1 (MQ=255)
                              ggCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCC.GCGCCGCAAGC.GTTTCCGAACCGGCATCATTCtt                                                           <  2:1391802/101‑1 (MQ=255)
                               gcgcgcGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCC.GCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTg                                                          <  1:1060503/101‑1 (MQ=255)
                               gcgcgcGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCC.GCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTg                                                          <  1:2752578/101‑1 (MQ=255)
                               gcgcgcGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCC.GCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTg                                                          <  2:705379/101‑1 (MQ=255)
                                cgcgcgGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCC.GCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTg.g                                                        >  2:2552900/1‑101 (MQ=255)
                                    cgGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCC.GCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTG.G.cccc                                                   >  1:2325392/1‑101 (MQ=255)
                                     ggACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCC.GCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTG.G.ccccc                                                  >  1:4467618/1‑101 (MQ=255)
                                      gACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCC.GCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTG.G.cccccc                                                 <  2:1771631/101‑2 (MQ=255)
                                      gACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCC.GCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTG.G.cccccc                                                 <  1:3847922/101‑2 (MQ=255)
                                      gACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCC.GCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTG.G.cccccc                                                 <  2:2162443/101‑2 (MQ=255)
                                       gcTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCC.GCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTG.G.ccccccg                                                <  2:2456216/100‑3 (MQ=255)
                                       aCTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCC.GCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTG.G.ccccccg                                                <  2:4197704/101‑3 (MQ=255)
                                         ttCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCC.GCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTG.G.ccccccgcc                                              >  2:3782285/1‑97 (MQ=255)
                                         ttCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCC.GCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTG.G.CCCcccccc                                              >  1:3627784/1‑100 (MQ=255)
                                          tCGGGGGGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATGGGCGACAA.TCCC.GCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTG.G.ccccccgccg                                             <  2:2907565/101‑6 (MQ=255)
                                          tCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCC.GCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTG.G.ccccccgccg                                             <  2:2249732/101‑6 (MQ=255)
                                           cGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCC.GCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTG.GCCCCCCGCCgg                                             >  2:1370319/1‑101 (MQ=255)
                                            ggggCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCC.GCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTG.GCCCCCCGCCggg                                            >  2:4023006/1‑101 (MQ=255)
                                            ggggCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCC.GCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTG.GCCCCCCGCCggg                                            <  2:553382/101‑1 (MQ=255)
                                             gggCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCC.GCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTG.GCCCCCCGCCGGGa                                           <  2:38508/101‑1 (MQ=255)
                                              ggcggcggcGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCC.GCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTG.GCCCCCCGCCGGGAc                                          <  1:858102/101‑1 (MQ=255)
                                              ggcggcggcGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCC.GCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTG.GCCCCCCGCCGGGAc                                          >  2:2932075/1‑101 (MQ=255)
                                               gcggcggcgGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCC.GCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTG.GCCCCCCGCCGGGACa                                         <  1:1974793/101‑1 (MQ=255)
                                               gcggcggcgGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCC.GCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTG.GCCCCCCGCCGGGACa                                         <  1:2683023/101‑1 (MQ=255)
                                                cggcggcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCC.GCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTG.GCCCCCCGCCGGGACaa                                        <  1:2544858/101‑1 (MQ=255)
                                                  gcggcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCC.GCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTG.GCCCCCCGCCGGGACAATc                                      <  2:1191635/101‑1 (MQ=255)
                                                   cggcggGCCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCC.GCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTG.GCCCCCCGCCGGGACAATCa                                     <  1:4587130/101‑1 (MQ=255)
                                                    ggcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCC.GCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTG.GCCCCCCGCCGGGACAATCAc                                    >  2:847507/1‑101 (MQ=255)
                                                    ggcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCC.GCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTG.GCCCCCCGCCGGGACAATCAc                                    <  2:376510/101‑1 (MQ=255)
                                                    gcgggggCTCTTGGCATCCTGCGTTTCCGGATTGGCGACAA.TCCC.GCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTG.GCCCCCCGCCGGGACAATCAc                                    <  2:3056579/98‑1 (MQ=255)
                                                     gcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCC.GCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTG.GCCCCCCGCCGGGACAATCACg                                   <  1:630600/101‑1 (MQ=255)
                                                       ggggCTCTTGCGATCCTGCGTTTCCGGGTTGGCGACAA.TCCC.GCGCCGCAAGCGG.TTCCGAACCGGCATCATTCTTG.GCCCCCCGCCGGGACAATCACGgc                                 >  2:4577377/1‑101 (MQ=255)
                                                       ggggCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCC.GCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTG.GCCCCCCGCCGGGACAATCACGgc                                 >  1:3053343/1‑101 (MQ=255)
                                                       ggggCTCTTGCGACCCTGCGTTTCCGGATTGGGGAAAA.TCCC.GCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGCC.CCCCCCCCGGGAAAATAACgac                                 >  2:3746061/1‑99 (MQ=255)
                                                        gggCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCC.GCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTG.GCCCCCCGCCGGGACAATCACGgcc                                <  1:4189371/101‑1 (MQ=255)
                                                         ggCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCC.GCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTG.GCCCCCCGCCGGGACAATCACGgccg                               >  2:1761181/1‑101 (MQ=255)
                                                          gCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCC.GCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTG.GCCCCCCGCCGGGACAATCACGgccgc                              >  2:1286473/1‑101 (MQ=255)
                                                          gCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCC.GCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTG.GCCCCCCGCCGGGACAATCACGgccgc                              >  2:1945221/1‑101 (MQ=255)
                                                          gCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCC.GCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTG.GCCCCCCACCGGGACAATCACGgccgc                              >  1:1660748/1‑101 (MQ=255)
                                                           ctctTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCC.GCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTG.GCCCCCCGCCGGGACAATCACGgccgcc                             >  1:1158090/1‑101 (MQ=255)
                                                           ctctTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCC.GCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTG.GCCCCCCGCCGGGACAATCACGgccgcc                             >  2:3664757/1‑101 (MQ=255)
                                                            tctTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCC.GCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTG.GCCCCCCGCCGGGACAATCACGGCCGCCt                            <  1:1734157/101‑1 (MQ=255)
                                                             ctTGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCC.GCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTG.GCCCCCCGCCGGGACAATCACGGCCGCCTc                           <  1:2643501/101‑1 (MQ=255)
                                                               tGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCC.GCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTG.GCCCCCCGCCGGGACAATCACGGCCGCCTCAt                         >  1:1868996/1‑101 (MQ=255)
                                                               tGCGATCCTGCGTTTCCGGATTGGCGACAA.TCCC.GCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTG.GCCCCCCGCCGGGACAATCACGGCCGCCTCAt                         >  2:1648723/1‑101 (MQ=255)
                                                                 cGATCCTGCGTTTCCGGATTGGCGACAA.TCCC.GCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTG.GCCCCCCGCCGGGACAATCACGGCCGCCTCATTg                       >  2:3392888/1‑101 (MQ=255)
                                                                 cGATCCTGCGTTTCCGGATTGGCGACAA.TCCC.GCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTG.GCCCCCCGCCGGGACAATCACGGCCGCCTCATTg                       >  1:3661469/1‑101 (MQ=255)
                                                                  gATCCTGCGTTTCCGGATTGGCGACAA.TCCC.GCGCCGCAAGC.GTTTCCGAACAGGCATCATTCTTG.GCCCCCCGCCGGGACAATCACGGCCGCCTCATTGt                      >  1:2370875/1‑101 (MQ=255)
                                                                   agccTGCGTTTCCGGATTGGCGACAA.TCCC.GCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTG.GCCCCCCGCCGGGACAATCACGGCCGCCTCATTGtt                     <  2:755356/99‑1 (MQ=255)
                                                                   aTCCTGCGTTTCCGGATTGGCGACAA.TCCC.CCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTG.GCCCCCCGCCGGGACAATCACGGCCGCCTCATTGtt                     <  1:1547658/101‑1 (MQ=255)
                                                                    tCCTGCGTTTCCGGATTGGCGACAA.TCCC.GCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTG.GCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTc                    <  1:2193385/101‑1 (MQ=255)
                                                                      cTGGGTTTCCGGATTGGCGACAA.T.CCAGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTG.GCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCg                  <  2:571415/101‑1 (MQ=255)
                                                                      cTGCGTTTCCGGATTGGCGACAA.TCCC.GCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTG.GCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCg                  >  2:4316216/1‑101 (MQ=255)
                                                                       tGCGTTTCCGGATTGGCGACAA.TCCC.GCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTG.GCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGc                 >  1:2223319/1‑101 (MQ=255)
                                                                        gCGTGTCCGGATGGGCGACAACC.CC.GCGCCGCAAGCGG.TTCCGAACCGGCGTCATTCTTG.GCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGcc                <  2:4625734/101‑1 (MQ=255)
                                                                        acGTTTCCGGATTGGCGACAA.TCCC.GCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTG.GCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGcc                <  2:382882/100‑1 (MQ=255)
                                                                         cGTTTCCGGATTGGCGACAA.TCCC.GCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTG.GCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCa               <  2:4467323/101‑1 (MQ=255)
                                                                         cGTTTCCGGATTGGCGACAA.TCCC.GCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTG.GCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCa               >  2:1448479/1‑101 (MQ=255)
                                                                           tttCCGGATTGGCGACAA.TCCC.GCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTG.GCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCAtt             <  2:3661201/101‑1 (MQ=255)
                                                                            ttCCGGATTGGCGACAA.TCCC.GCGCCGCAAGC.GTTTCCGAACCGGCCTCATTCTTG.GCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTg            <  2:2157610/101‑1 (MQ=255)
                                                                             tCCGGATTGGCGACAA.TCCC.GCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTG.GCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGc           >  2:4067450/1‑101 (MQ=255)
                                                                               cGGATTGGCGACAA.TCCC.GCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTG.GCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGa         >  1:3788859/1‑101 (MQ=255)
                                                                                 gATTGGCGACAA.TCCC.GCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTG.GCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATc       >  2:3708322/1‑101 (MQ=255)
                                                                                 gATTGGCGACAA.TCCC.GCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTG.GCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATc       <  1:4507267/101‑1 (MQ=255)
                                                                                  aTTGGCGACAA.TCCC.GCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTG.GCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCa      >  1:4395996/1‑101 (MQ=255)
                                                                                   ttGGCGACAA.TCCC.GCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTG.GCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCaa     >  1:2127862/1‑101 (MQ=255)
                                                                                   ttGGCGACAA.TCCC.GCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTG.GCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCaa     >  2:2285689/1‑101 (MQ=255)
                                                                                    tGGCGACAA.TCCC.GCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTG.GCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCaaa    <  1:3018620/101‑1 (MQ=255)
                                                                                    tGGCGACAA.TCCC.GCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTG.GCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCaaa    <  1:43428/101‑1 (MQ=255)
                                                                                     ggCGACAA.TCCC.GCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTG.GCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCAAAg   <  1:2487176/101‑1 (MQ=255)
                                                                                      gCGACAA.TCCC.GCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTG.GCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCAAAGc  <  1:957601/101‑1 (MQ=255)
                                                                                                      |                                                                                        
GTCCTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAA.T.CC.GCG.CGC.AGC.G.TTCCGAACCGGCATCAATCTTG.G.CCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCAAAGC  >  NC_014034/2057732‑2057913

Base quality scores:  ATCG  < 3 ≤  ATCG  < 16 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG