Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,070,633	+G	100%	intergenic (+48/+124)	dcd → / ← guaB	deoxycytidine triphosphate deaminase/inosine‑5'‑monophosphate dehydrogenase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,070,633	1	.→G	100.0%	63.5	22	intergenic (+48/+124)	dcd/guaB	deoxycytidine triphosphate deaminase/inosine‑5'‑monophosphate dehydrogenase
Reads supporting (aligned to +/- strand):  new base (1/20):  ref base (1/0):  total (2/20)

GAACTATCAGGGCCAGGGGCTGAAGCTCGCCAAGCATTTCCGCGCGGGGTGAGGGG.CGCGCGGATGGATTTCGCCGCGGCTTTGCCTGCGGCGACCCGCC.GGGGGCGCTG..CCCCCCGGACCCCCCGGGATATTTTCGGCAAGATGAAGGGGAAGCGGTGTTTGCCGGTACACAGAAAAAGGGCCGGGGTTTCCC  >  NC_014034/2070534‑2070727                                                                                                      |                                                                                                 gAACTATCAGGGCCAGGGGCTGAAGCTCGCCAAGCATTTCCGCGCGGGGTGAGGGG.CGCGCGGATGGATTTCGCCGCGGCTTTGCCTGCGGCGACccgccc                                                                                                  >  2:2571051/1‑100 (MQ=255) gAACTATCAGGGCCAGGGGCCGAAGCTCGCCAAGCATTTCCGCGCGGGGTGAGGGG.CGCGCGGATGGATTTCGCCGCGGCTTTGCCTGCGGCGACccgccc                                                                                                  >  1:1282305/1‑100 (MQ=255)  aaCTATCAGGGCCAGGGGCTGAAGCTCGCCAAGCATTTCCGCGCGGGGTGAGGGG.CGCGCGGATGGATTTCGCCGCGGCTTTGCCTGCGGCGACccgcctg                                                                                                 >  2:4345318/1‑99 (MQ=255)    cTATCAGGGCCAGGGGCTGAAGCTCGCCAAGCATTTCCGCGCGGGGTGAGGGG.CGCGCGGATGGATTTCGCCGCGGCTTTGCCTGCGGCGACCCGCC.gggg                                                                                              >  1:1193159/1‑101 (MQ=255)       tCAGGGCCAGGGGCTGAAGCTCGCCAAGCATTTCCGCGCGGGGTGAGGGG.CGCGCGGATGGATTTCGCCGCGGCTTTGCCTGCGGCGACCCGCC.ggggggg                                                                                           >  1:2194834/1‑99 (MQ=255)       tCAGGGCCAGGGGCTGAAGCTCGCCAAGCATTTCCGCGCGGGGTGAGGGG.CGCGCGGATGGATTTCGCCGCGGCTTTGCCTGCGGCGACCCGCC.ggggggc                                                                                           >  1:4528123/1‑99 (MQ=255)       ccggggCGAGGGGCTGAAGCTCCCAAAGCTTTCCCGGGGGGGGTGGGGGG.GGCGCGGATGTTTTTCGCCGCGGTTTTGCCGGCGGCGACCCGCC.ggggggc                                                                                           <  2:596377/98‑3 (MQ=255)           ggCCGGGGGCTGAAGCTCGCCAAGCATTTCCGCGTGGGGTGAGGGG.CGCGCGGATGGATTTCGCCGCGGCTTTGCCTGCGCCGACCCGCCGGGGGGCGCTg                                                                                        <  1:4224553/101‑1 (MQ=255)                 gggCTGAAGCTCGCCAAGCATTTCCGCGCGGGGTGAGGGG.CGCGCGGATGGATTTCGCCGCGGCTTTGCCTGCGGCGACCCGCC.TGGGgcggggccggctc                                                                                 >  2:1172327/1‑91 (MQ=39)                  ggcggacccccGCCAAGCGTTCCGGCGGGGGGGGGGGGG.CGCGGGGATGGTTTCCGCCGCGGTTTTCCCGGCGGCGCCCCGCCGGGGGGCGCTG..CCCCCCg                                                                               <  1:4372146/91‑1 (MQ=255)                   gCTGAACCCCGCCAAGCTTTCCCGGGGGGGGGGGGGGG.CGCGGGGGTGGTTTCCCCCGCGGCTTTGCCGGCGGCCCCCCGCCGGGGGGCGCTG..CCCCCCgg                                                                              <  1:3361404/101‑1 (MQ=255)                   gCTAAAGCCCGCCAGGCATTCCCGGGGGGGGGGGGGGG.CGCGGGGATGTTTTCCGCCGCGTCTTTGCCGGCGGCGCCCCGCCGGGGGGCGCTG..CCCCCCgg                                                                              <  2:4527828/101‑1 (MQ=255)                    gtGAGGCGCGCCCAGCCTTCCCGCGGGGGGTGGGGGGTCGGGGGGA.GTATTTCCCCGCGGTTTCGCGCGCGGAACCCCGCCGGGGGGCGCTG..CCCCCCGGa                                                                             <  1:3091436/100‑1 (MQ=255)                    gtGAAGCTCCCCAGGCTTTCCCGGGGGGGGGGGGGGG.CGCGGGGAGTGTTTCCGCCGCGTTTTTCCCGGCGGCGCCCCGCCGGGGGGCGCTG..CCCCCCGGa                                                                             <  1:2066940/100‑1 (MQ=255)                     ggAAGCCCCCAAAGCTTTCCCGGGGGGGGGGGGGGG.CGCGGGGATGTTTTCCGCCGCGGTTTGCCCGGCGGCGCCCCGCCGGGGGGCGCTG..CCCCCCGGAc                                                                            <  1:468234/100‑1 (MQ=255)                        ggccccccAGGCATTGGCGGGGGGGGTGGGGGG.CGGGGGGATGGTTTCCCCCGCGTCTTTCCCTGCGGCGCCCCGCCGGGGGGCGCTG..CCCCCCGGAcccc                                                                         <  2:1480453/95‑1 (MQ=12)                         gcgcGCCAAGCTTCGCCGCGCGGGGTGCGGCG.CGCGCGGTTGGTTTGCGCCGGGGCTTTCCCGGGGGCCCCCCGCCGGGGGGCGCTG..CCCCCCGGAccccc                                                                        <  2:1503508/98‑1 (MQ=12)                          cTCCCCAAGCGTTCCGGGGGGGGGGGGGGGG.CGCGGGGGTGGTTTCCGCCGCGGCTTTGCCGGCGGCGCCCCGCCGGGGGGCGCTG..CCCCCCGGAcccccc                                                                       <  2:1046899/101‑1 (MQ=16)                                 aGCATTTCCGCGCGGGGTGAGGGG.CGCGCGGATGGATTTCGCCGCGGCTTTGCCTGCGGCGACCCGCCGGGGGGGGGCG..CCCCCCCGAcacccccgtgtct                                                                >  2:568355/1‑94 (MQ=25)                                    aTTTCCGCGCGGGGTGAGGGG.CGCGCGGATGGATTTCGCCGCGGCTTTGCCTGCGGCGACCCGCC.GGGGGGGGGGGCCCCCCCGGCCCCCCCgccgagatt                                                              >  2:3044974/1‑93 (MQ=25)                                              ggggGGGGGGG.CGCGGGGGTGGTTTCCGCCCCGTTTTGGCCGGCGGCCCCCCGCCGGGGGGCGCTG..CCCCCCGGACCCCCCGGGATATTTTCGGCAAGATg                                                   <  2:3748582/101‑1 (MQ=9)                                                    aGGGG.CGCGCGGATGGATTTCGCCGCGGCTTTGCCTGCGGCGACCCGCCGGGGGGCGGTG..CCCCCCGGACCCCCCGGGGTATTTGTGGCAAGATGAGgggg                                             >  2:1595805/1‑101 (MQ=37)                                                                  gggtttccgccgcGGGTCTGCCTGGGGCCCCCCGCCGGGGGGCGCTG..CCCCCCGGACCCCCCGGGATATTTTCGGCAAGATGAAGGGGAAGCGGTGTTTGc                                <  2:4568925/94‑1 (MQ=18)                                                                   gtttcccgccgcGGTTTGCCCGGCGCCCCCCCGCCGGGGGGCGCTG..CCCCCCGGACCCCCCGGGATATTTTCGGCAAGATGAAGGGGAAGCGGTGTTTGcc                               <  1:4497306/95‑1 (MQ=18)                                                                        tCGCCGCGGTTTCGCCGGCGCCCCCCCGCCGGGGGGCGCTG..CCCCCCGGACCCCCCGGGATATTTTCGGCAAGATGAAGGGGAAGCGGTGTTTGCCGGTac                          <  2:1227262/101‑1 (MQ=21)                                                                         ccccccgGCTTTGCCGGCGGCGCCCCGCCGGGGGGCGCTG..CCCCCCGGACCCCCCGGGATATTTTCGGCAAGATGAAGGGGAAGCGGTGTTTGCCGGTaca                         <  2:2643686/96‑1 (MQ=25)                                                                           ccgcGTCTTTCCCGGGGGCCCCCCGCCGGGGGGCGCTG..CCCCCCGGACCCCCCGGGATATTTTCGGCAAGATGAAGGGGAAGCGGTGTTTGCCGGTAcaca                       <  2:3909367/101‑1 (MQ=21)                                                                              cgGCTGTGCCCGCGCCGCCCCGCCGGGGGGCGCTG..CCCCCCGGACCCCCCGGGATATTTTCGGCAAGATGAAGGGGAAGCGGTGTTTGCCGGTACACAGaa                    <  1:3742455/101‑1 (MQ=25)                                                                                      cgcgcggccCCCCGCCGGGGGGCGCTG..CCCCCCGGACCCCCCGGGATATTTTCGGCAAGATGAAGGGGAAGCGGTGTTTGCCGGTACACAGAAAAAGGGcc            <  1:3525005/98‑1 (MQ=25)                                                                                      ccggcgcccccccGCCGGGGGGCGCTG..CCCCCCGGACCCCCCGGGATATTTTCGGCAAGATGAAGGGGAAGCGGTGTTTGCCGGTACACAGAAAAAGGGcc            <  1:3130026/91‑1 (MQ=25)                                                                                                cccGCCGGGGGGCGCTG..CCCCCCGGACCCCCCGGGATATTTTCGGCAAGATGAAGGGGAAGCGGTGTTTGCCGGTACACAGAAAAAGGGCCGGGGTTTccc  <  1:3742480/101‑1 (MQ=255)                                                                                                      |                                                                                                 GAACTATCAGGGCCAGGGGCTGAAGCTCGCCAAGCATTTCCGCGCGGGGTGAGGGG.CGCGCGGATGGATTTCGCCGCGGCTTTGCCTGCGGCGACCCGCC.GGGGGCGCTG..CCCCCCGGACCCCCCGGGATATTTTCGGCAAGATGAAGGGGAAGCGGTGTTTGCCGGTACACAGAAAAAGGGCCGGGGTTTCCC  >  NC_014034/2070534‑2070727 Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 30 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG