Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,162,477 +A 100% intergenic (+210/‑247) RCAP_rcc02005 → / → RCAP_rcc02006 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,162,4771.→A100.0% 846.3 221intergenic (+210/‑247)RCAP_rcc02005/RCAP_rcc02006hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (95/123):  ref base (1/0):  total (98/123)

Only 100 of 252 total aligned reads displayed.

TCGTCACCCTCCGAAACCTCGCCGCGGATCTCGATTGATTTCAGGGCGCTCCAGTGGTAAAGGAGCACATCACCGCCAAAATGACGGCGGACTGAACCTAAG.A.CGGGGACAGTCGACCAGAAACCCCAAGGAGCACATCACCGCCAAAATGACGGCGGACTGAACCTGCCGGGTCTCATTGAGGATAAGGGCAATCG  >  NC_014034/2162376‑2162572
                                                                                                      |                                                                                                
 cgtcACCCTCCGAAACCT.GCCGCGGATCTCGATTGATTTCAGGGCGTTCCAGTGGTAAAGGAGCACATCACCGCCAAAATGACGGCGGACTGAACCTAAG.a                                                                                                 >  1:2745739/1‑101 (MQ=255)
 cgtcACCCTCCGAAACCT.GCCGCGGATCTCGATTGATTTCAGGGCGTTCCAGTGGTAAAGGAGCACATCACCGCCAAAATGACGGCGGACTGAACCTAAG.a                                                                                                 <  1:217310/101‑1 (MQ=255)
  gtcACCCTCCGAAACCT.GCCGCGGATCTCGATTGATTTCAGGGCGTTCCAGTGGTAAAGGAGCACATCACCGCCAAAATGACGGCGGACTGAACCTAAG.aa                                                                                                >  1:1208549/1‑100 (MQ=255)
   tcccccTCCGAAACCT.GCCGCGGATCTCGANTGATTTCAGGGCGTTCCAGTGGTAAAGGAGCACATCACCGCCAAAATGACGGCGGACTGAACCTAAG.aac                                                                                               <  1:52106/98‑3 (MQ=37)
   tcACCCTCCGAAACCT.GCCGCGGATCTCGATTGATTTCAGGGCGTTCCAGTGGTAAAGGAGCACATCACCGCCAAAATGACGGCGGACTGAACCTAAG.aac                                                                                               <  2:4002361/101‑3 (MQ=255)
    cACCCTCCGAAACCT.GCCGCGGATCTCGATTGATTTCAGGGCGTTCCAGTGGTAAAGGAGCACATCACCGCCAAAATGACGGCGGACTGAACCTAAG.aacg                                                                                              >  1:3628274/1‑98 (MQ=255)
       ccTCCGAAACCT.GCCGCGGATCTCGATTGATTTCAGGGCGTTCCAGTGGTAAAGGAGCACATCACCGCCAAAATGACGGCGGACTGAACCTAAGAA.Cgggg                                                                                           >  2:4494409/1‑101 (MQ=37)
       ccTCCGAAACCT.GCCGCGGATCTCGATTGATTTCAGGGCGTTCCAGTGGTAAAGGAGCACATCACCGCCAAAATGACGGCGGACTGAACCTAAGAA.Cgggg                                                                                           <  1:4384316/101‑1 (MQ=37)
        cTCCGAAACCT.GCCGCGGATCTCGATTGATTTCAGGGCGTTCCAGTGGTAAAGGAGCACATCACCGCCAAAATGACGGCGGACTGAACCTAAGAA.Cggggc                                                                                          >  2:1397148/1‑100 (MQ=37)
         cccGAAACCT.GCCGCGGATCTCGATTGATTTCAGGGCGTTCCAGTGGTAAAGGAGCACATCACCGCCAAAATGACGGCGGACTGAACCTAAGAA.CGGGGAc                                                                                         <  1:3041424/100‑1 (MQ=37)
          ccGAAACCT.GCCGCGGATCTCGATTGATTTCAGGGCGTTCCAGTGGTAAAGGAGCACATCACCGCCAAAATGACGGCGGACTGAACCTAAGAA.CGGGGACa                                                                                        <  1:816349/101‑1 (MQ=37)
          ccGAAACCT.GCCGCGGATCTCGATTGATTTCAGGGCGTTCCAGTGGTAAAGGAGCACATCACCGCCAAAATGACGGCGGACTGAACCTAAGAA.CGGGGACa                                                                                        <  1:3588130/101‑1 (MQ=37)
           cGAAACCT.GCCGCGGATCTCGATTGATTTCAGGGCGTTCCAGTGGTAAAGGAGCACATCACCGCCAAAATGACGGCGGACTGAACCTAAGAA.CGGGGACAg                                                                                       <  2:4595306/101‑1 (MQ=37)
           cGAAACCT.GCCGCGGATCTCGATTGATTTCAGGGCGTTCCAGTGGTAAAGGAGCACATCACCGCCAAAATGACGGCGGACTGAACCTAAGAA.CGGGGACAg                                                                                       <  1:735210/101‑1 (MQ=37)
           cGAAACCT.GCCGCGGATCTCGATTGATTTCAGGGCGTTCCAGTGGTAAAGGAGCACATCACCGCCAAAATGACGGCGGACTGAACCTAAGAA.CGGGGACAg                                                                                       >  2:347933/1‑101 (MQ=37)
           cGAAACCT.GCCGCGGATCTCGATTGATTTCAGGGCGTTCCAGTGGTAAAGGAGCACATCACCGCCAAAATGACGGCGGACTGAACCTAAGAA.CGGGGACAg                                                                                       <  1:104237/101‑1 (MQ=37)
            gAAACCT.GGCGCGGAGCTCGATGGAGTTCAGGTGGTGCCAGTGGTAAAGGAGCACATCACCGCCAAAATGACGGCGGACTGAACCTAAGAA.CGGGGACAGt                                                                                      <  1:3762773/101‑1 (MQ=18)
            gAAACCT.GCCGCGGATCTCGATTGATTTCAGGGCGTTCCAGTGGTAAAGGAGCACATCACCGCCAAAATGACGGCGGACTGAACCTAAGAA.CGGGGACAGt                                                                                      <  1:2847670/101‑1 (MQ=37)
            gAAACCT.GCCGCGGATCTCGATTGATTTCAGGGCGTTCCAGTGGTAAAGGAGCACATCACCGCCAAAATGACGGCGGACTGAACCTAAGAA.CGGGGACAGt                                                                                      >  2:2176874/1‑101 (MQ=37)
             gaaccctgccgcGGATCTCGATTGATTTCAGGGCGTTCCAGTGGTAAAGGAGCACATCACCGCCAAAATGACGGCGGACTGAACCTAAGAA.CGGGGACAGt                                                                                      <  1:2694850/94‑1 (MQ=25)
             aaaCCT.GCCGCGGATCTCGATTGATTTCAGGGCGTTCCAGTGGTAAAGGAGCACATCACCGCCAAAATGACGGCGGACTGAACCTAAGAA.CGGGGACAGTc                                                                                     <  2:1291040/101‑1 (MQ=37)
             aaaCCT.GCCGCGGATCTCGATTGATTTCAGGGCGTTCCAGTGGTAAAGGAGCACATCACCGCCAAAATGACGGCGGACTGAACCTAAGAA.CGGGGACAGTc                                                                                     <  1:2951074/101‑1 (MQ=37)
             aaaCCT.GCCGCGGATCTCGATTGATTTCAGGGCGTTCCAGTGGTAAAGGAGCACATCACCGCCAAAATGACGGCGGACTGAACCTAAGAA.CGGGGACAGTc                                                                                     <  1:3894402/101‑1 (MQ=37)
                 cctgccgcGGATCTCGATTGATTTCAGGGCGTTCCAGTGGTAAAGGAGCACATCACCGCCAAAATGACGGCGGACTGAACCTAAG.AGCGGGGACAGTCGAc                                                                                  >  1:4021666/4‑101 (MQ=37)
                    gccgcGGATCTCGATTGATTTCAGGGCGTTCCAGTGGTAAAGGAGCACATCACCGCCAAAATGACGGCGGACTGAACCTAAGAA.CGGGGACAGTCGACCAg                                                                               <  2:3548589/101‑1 (MQ=255)
                       gcgGATCTCGATTGATTTCAGGGCGTTCCAGTGGTAAAGGAGCACATCACCGCCGAAATGACGGCGGACTGAACCTAAGAA.CGGGGACAGTCGACCAGaaa                                                                            >  2:1648543/1‑101 (MQ=37)
                       gcgGATCTCGATTGATTTCAGGGCGTTCCAGTGGTAAAGGAGCACATCACCGCCAAAATGACGGCGGACTGAACCTAAGAA.CGGGGACAGTCGACCAGaaa                                                                            <  1:1268446/101‑1 (MQ=255)
                        cgGATCTCGATTGATTTCAGGGCGTTCCAGTGGTAAAGGAGCACATCACCGCCAAAATGACGGCGGACTGAACCTAAGAA.CGGGGACAGTCGACCAGAAAc                                                                           >  1:208698/1‑101 (MQ=255)
                          gATCTCGATTGATTTCAGGGCGTTCCAGTGGTAAAGGAGCACATCACCGCCAAAATGACGGCGGACTGAACCTAAGAA.CGGGGACAGTCGACCAGAAAccc                                                                         <  1:3792669/101‑1 (MQ=255)
                          gATCTCGATTGATTTCAGGGCGTTCCAGTGGTAAAGGAGCACATCACCGCCAAAATGACGGCGGACTGAACCTAAGAA.CGGGGACAGTCGACCAGAAAacc                                                                         >  2:366575/1‑101 (MQ=37)
                            tctcGATTGATTTCAGGGCGTTCCAGTGGTAAAGGAGCACATCACCGCCAAAATGACGGCGGACTGAACCTAAGAA.CGGGGACAGTCGACCAGAAACCCCa                                                                       <  1:3181804/101‑1 (MQ=255)
                              tcGATTGATTTCAGGGCGTTCCAGTGGTAAAGGAGCACATCACCGCCAAAATGACGGCGGACTGAACCTAAGAA.CGGGGACAGTCGACCAGAAACCCCAAg                                                                     <  2:3837148/101‑1 (MQ=255)
                              tcGATTGATTTCAGGGCGTTCCAGTGGTAAAGGAGCACATCACCGCCAAAATGACGGCGGACTGAACCTAAGAA.CGGGGACAGTCGACCAGAAACCCCAAg                                                                     >  1:3408516/1‑101 (MQ=255)
                              tcGATTGATTTCAGGGCGTTCCAGTGGTAAAGGAGCACATCACCGCCAAAATGACGGCGGACTGAACCTAAGAA.CGGGGACAGTCGACCAGAAACCCCAAg                                                                     >  1:3858458/1‑101 (MQ=255)
                               cGATTGATTTCAGGGCGTTCCAGTGGTAAAGGAGCACATCACCGCCAAAATGACGGCGGACTGAACCTAAGCA.CGGGGACATTCGACCAGAAACCCCAAgc                                                                    >  2:633418/1‑100 (MQ=37)
                                gattgattTCAGGGCGTTCCAGTGGTAAAGGAGCACATCACCGCCAAAATGACGGCGGACTGAACCTAAGAA.CGGGGACAGTCGACCAGAAACCCCAAGGa                                                                   <  2:230152/101‑1 (MQ=255)
                                gattgattTCAGGGCGTTCCAGTGGTAAAGGAGCACATCACCGCCAAAATGACGGCGGACTGAACCTAAGAA.CGGGGACAGTCGACCAGAAACCCCAAGGa                                                                   <  2:2840642/101‑1 (MQ=255)
                                 attgattTCAGGGCGTTCCAGTGGTAAAGGAGCACATCACCGCCAAAATGACGGCGGACTGAACCTAAGAA.CGGGGACAGTCGACCAGAAACCCCAAGGAg                                                                  >  1:2397020/1‑101 (MQ=255)
                                       ttCAGGGCGTTCCAGTGGTAAAGGAGCACATCACCGCCAAAATGACGGCGGACTGAACCTAAGAA.CGGGGACAGTCGACCAGAAACCCCAAGGAGCACATc                                                            >  1:2113214/1‑101 (MQ=255)
                                       ttCAGGGCGTTCCAGTGGTAAAGGAGCACATCACCGCCAAAATGACGGCGGACTGAACCTAAGAA.CGGGGACAGTCGACCAGAAACCCCAAGGAGCACATc                                                            >  1:3084266/1‑101 (MQ=255)
                                          aGGGCGTTCCAGTGGTAAAGGAGCACATCACCGCCAAAATGACGGCGGACTGAACCTAAGAA.CGGGGACAGTCGACCAGAAACCCCAAGGAGCACATCAcc                                                         >  1:4615992/1‑101 (MQ=255)
                                            ggCGTTCCAGTGGTAAAGGAGCACATCACCGCCAAAATGACGGCGGACTGAACCTAAGAA.CGGGGACAGTCGACCAGAAACCCCAAGGAGCACATCACCGc                                                       >  2:2663769/1‑101 (MQ=255)
                                             gcgtTCCAGTGGTAAAGGAGCACATCACCGCCAAAATGACGGCGGACTGAACCTAAGAA.CGGGGACAGTCGACCAGAAACCCCAAGGAGCACATCACCGcc                                                      <  1:1525530/101‑1 (MQ=255)
                                              cgttCCAGTGGTAAAGGAGCACATCACCGCCAAAATGACGGCGGACTGAACCTAAGAA.CGGGGACAGTCGACCAGAAACCCCAAGGCGCACATCACCGCCa                                                     >  2:2150706/4‑101 (MQ=37)
                                               gttCCAGTGGTAAAGGAGCACATCACCGCCAAAATGACGGCGGACTGAACCTAAGAA.CGGGGACAGTCGACCAGAAACCCCAAGGAGCACATCACCGCCaa                                                    >  1:2181891/3‑101 (MQ=255)
                                                ttCCATTGGTAAAGGAGCACATCACCGCCAAAATGACGGCGGACTGAACCTAAGAA.CGGGGACAGTCGACCAGAAACCCCAAGGAGCACATCACCGCCaaa                                                   >  2:4182020/2‑101 (MQ=255)
                                                 tCCAGTGGTAAAGGAGCACATCACCGCCAAAATGACGGCGGACTGAACCTAAGAA.CGGGGACAGTCGACCAGAAACCCCAAGGAGCACATCACCGGCaaaa                                                  >  2:1937076/1‑101 (MQ=255)
                                                 tCCAGTGGTAAAGGAGCACATCACCGCCAAAATGACGGCGGACTGAACCTAAGAA.CGGGGACAGTCGACCAGAAACCCCAAGGAGCACATCACCGCCaaaa                                                  <  1:1816872/101‑1 (MQ=255)
                                                   cAGTGGTAAAGGAGCACATCACCGCCAAAATGACGGCGGACTGAACCTAAGAA.CGGGGACAGTCGACCAGAAACCCCAAGGAGCACATCACCGCCAAAATg                                                <  1:3471791/101‑1 (MQ=255)
                                                   cAGTGGTAAAGGAGCACATCACCGCCAAAATGAAGGCGGACTGAACCTAAGAA.CGGGGACAGTCTACCAGAAACCCCAAGGAGCACATCACCGCCAAAATg                                                >  2:3054411/1‑101 (MQ=37)
                                                    aGTGGTAAAGGAGCACATCACCGCCAAAATGACGGCGGACTGAACCTAAGAA.CGGGGACAGTCGACCAGAAACCCCAAGGAGCACATCACCGCCAAAATGa                                               <  1:1720545/101‑1 (MQ=255)
                                                    aGTGGTAAAGGAGCACATCACCGCCAAAATGACGGCGGACTGAACCTAAGAA.CGGGGACAGTCGACCAGAAACCCCAAGGAGCACATCACCGCCAAAATGa                                               >  2:2218679/1‑101 (MQ=255)
                                                    aGTGGTAAAGGAGCACATCACCGCCAAAATGACGGCGGACTGAACCTAAGAA.CGGGGACAGTCGACCAGAAACCCCAAGGAGCACATCACCGCCAAAATGa                                               >  2:2692801/1‑101 (MQ=255)
                                                     gTGGTAAAGGAGCACATCACCGCCAAAATGACGGCGGACTGAACCTAAGAA.CGGGGACAGTCGACCAGAAACCCCAAGGAGCACATCACCGCCAAAATGAc                                              <  2:3575194/101‑1 (MQ=255)
                                                        gTAAAGGAGCACATCACCGCCAAAATGACGGCGGACTGAACCTAAGAA.CGGGGACAGTCGACCAGAAACCCCAAGGAGCACATCACCGCCAAAATGAcggc                                           <  2:71280/101‑1 (MQ=255)
                                                         tAAAGGAGCACATCACCGCCAAAATGACGGCGGACTGAACCTAAGAA.CGGGGACAGTCGACCAGAAACCCCAAGGAGCACATCACCGCCAAAATGAcggcg                                          >  2:3095341/1‑101 (MQ=255)
                                                         tAAAGGAGCACATCACCGCCAAAATGACGGCGGACTGAACCTAAGAA.CGGGGACAGTCGACCAGAAACCCCAAGGAGCACATCACCGCCAAAATGAcggcg                                          >  1:2514705/1‑101 (MQ=255)
                                                           aaGGAGCACATCACCGCCAAAATGACGGCGGACTGAACCTAAGAA.CGGGGACAGTCGACCAGAAACCCCAAGGAGCACATCACCGCCAAAATGACGGCGGa                                        <  2:1878147/101‑1 (MQ=255)
                                                           aaGGAGCACATCACCGCCAAAATGACGGCGGACTGAACCTAAGAA.CGGGGACAGTCGACCAGAAACCCCAAGGAGCACATCACCGCCAAAATGACGGCGGa                                        >  2:20185/1‑101 (MQ=255)
                                                            agtatcacaTCACCGCCAACATGACGGCGGACTGAACCTAAGAA.CGGAGACAGTCGACCAGAAACCCCAAGGAGCACATCACCGCCAAAATGACGGCGGAc                                       <  2:3260484/96‑1 (MQ=37)
                                                             ggAGCACATCACCGCCAAAATGACGGCGGACTGAACCTAAGAA.CGGGGACAGTCGACCAGAAACCCCAAGGAGCACATCACCGCCAAAATGACGGCGGACt                                      <  2:292465/101‑1 (MQ=255)
                                                              gAGCACATCACCGCCAAAATGACGGCGGACTGAACCTAAGAA.CGGGGACAGTCGACCAGAAACCCCAAGGAGCACATCACCGCCAAAATGACGGCGGACTg                                     <  1:4528181/101‑1 (MQ=255)
                                                              gAGCACATCACCGCCAAAATGACGGCGGACTGAACCTAAGAA.CGGGGACAGTCGACCAGAAACCCCAAGGAGCACATCACCGCCAAAATGACGGCGGACTg                                     >  1:265198/1‑101 (MQ=255)
                                                               aGCACATCACCGCCAAAATGACGGCGGACTGAACCTAAGAA.CGGGGACAGTCGACCAGAAACCCCAAGGAGCACATCACCGCCAAAATGACGGCGGACTGa                                    <  2:3974561/101‑1 (MQ=255)
                                                               aGCACATCACCGCCAAAATGACGGCGGACTGAACCTAAGAA.CGGGGACAGTCGACCAGAAACCCCAAGGAGCACATCACCGCCAAAATGACGGCGGACTGa                                    >  1:2275499/1‑101 (MQ=255)
                                                               aGCACATCACCGCCAAAATGACGGCGGACTGAACCTAAGAA.CGGGGACAGTCGACCAGAAACCCCAAGGAGCACATCACCGCCAAAATGACGGCGGACTGa                                    >  1:2879799/1‑101 (MQ=255)
                                                                gCACATCACCGCCAAAATGACGGCGGACTGAACCTAAGAA.CGGGGACAGTCGACCAGAAACCCCAAGGAGCACATCACCGCCAAAATGACGGCGGACTGaa                                   <  2:1219836/101‑1 (MQ=255)
                                                                gCACATCACCGCCAAAATGACGGCGGACTGAACCTAAGAA.CGGGGACAGTCGACCAGAAACCCCAAGGAGCACATCACCGCCAAAATGACGGCGGACTGaa                                   <  1:417519/101‑1 (MQ=255)
                                                                  ccaTCACCGCCAAAATGACGGCGGACTGAACCTAAGAA.CGGGGACAGTCGACCAGAAACCCCAAGGAGCACATCACCGCCAAAATGACGGCGGACTGAAcc                                 <  2:941646/100‑1 (MQ=255)
                                                                  acaTCACCGCCAAAATGACGGCGGACTGAACCTAAGCA.CGGGGACAGGCGACCAGAAACCCCAAGGAGAACATCACCGCCAAAATGACGGCGGACTGCAcc                                 >  2:514049/1‑101 (MQ=38)
                                                                    aTCACCGCCAAAATGACGGCGGACTGAACCTAAGAA.CGGGGACAGTCGACCAGAAACCCCAAGGAGCACATCACCGCCAAAATGACGGCGGACTGAACCTg                               <  2:430897/101‑1 (MQ=255)
                                                                            cAAAATGACGGCGGACTGAACCTAAGAA.CGGGGACAGTCGACCAGAAACCCCAAGGAGCACATCACCGCCAAAATGACGGCGGACTGAACCTGCCGGGtct                       >  2:3035159/1‑101 (MQ=255)
                                                                             aaaaTGACGGCGGACTGAACCTAAGAA.CGGGGACAGTCGACCAGAAACCCCAAGGAGCACATCACCGCCAAAATGACGGCGGACTGAACCTGCCGGGtctc                      >  1:566296/1‑101 (MQ=255)
                                                                             aaaaTGACGGCGGACTGAACCTAAGAA.CGGGGACAGTCGACCAGAAACCCCAAGGAGCACATCACCGCCAAAATGACGGCGGACTGAACCTGCCGGGtctc                      <  1:3490592/101‑1 (MQ=255)
                                                                             aaaaTGACGGCGGACTGAACCTAAGAA.CGGGGACAGTCGACCAGAAACCCCAAGGAGCACATCACCGCCAAAATGACGGCGGACTGAACCTGCCGGGtctc                      <  1:3698675/101‑1 (MQ=255)
                                                                               aaTGACGGCGGACTGAACCTAAGAA.CGGGGACAGTCGACCAGAAACCCCAAGGAGCACATCACCGCCAAAATGACGGCGGACTGAACCTGCCGGGTCTCAt                    <  2:4288667/101‑1 (MQ=255)
                                                                                aTGACGGCGGACTGAACCTAAGAA.CGGGGACAGTCGACCAGAAACCCCAAGGAGCACATCACCGCCAAAATGACGGCGGACTGAACCTGCCGGGTCTCAtt                   <  1:1791449/101‑1 (MQ=255)
                                                                                aTGACGGCGGACTGAACCTAAGAA.CGGGGACAGTCGACCAGAAACCCCAAGGAGCACATCACCGCCAAAATGACGGCGGACTGAACCTGCCGGGTCTCAtt                   >  2:1080953/1‑101 (MQ=255)
                                                                                 tGACGGCGGACTGAACCTAAGAA.CGGGGACAGTCGACCAGAAACCCCAAGGAGCACATCACCGCCAAAATGACGGCGGACTGAACCTGCCGGGTCTCATTg                  <  2:2605534/101‑1 (MQ=255)
                                                                                   aCGGCGGACTGAACCTAAGAA.CGGGGACAGTCGACCAGAAACCCCAAGGAGCACATCACCGCCAAAATGACGGCGGACTGAACCTGCCGGGTCTCATTGAg                <  2:4070920/101‑1 (MQ=255)
                                                                                    cggcggACTGAACCTAAGAA.CGGGGACAGTCGACCAGAAACCCCAAGGAGCACATCACCGCCAAAATGACGGCGGACTGAACCTGCCGGGTCTCATTGAgg               <  2:751845/101‑1 (MQ=255)
                                                                                     ggcggACTGAACCTAAGAA.CGGGGACAGTCGACCAGAAACCCCAAGGAGCACATCACCGCCAAAATGACGGCGGACTGAACCTGCCGGGTCTCATTGAGGa              <  1:1412206/101‑1 (MQ=255)
                                                                                     ggcggACTGAACCTAAGAA.CGGGGACAGTCGACCAGAAACCCCAAGGAGCACATCACCGCCAAAATGACGGCGGACTGAACCTGCCGGGTCTCATTGAGGa              >  2:1694876/1‑101 (MQ=255)
                                                                                       cggACTGAACCTAAGAA.CGGGGACAGTCGACCAGAAACCCCAAGGAGCACATCACCGCCAAAATGACGGCGGACTGAACCTGCCGGGTCTCATTGAGGATa            >  1:1300245/1‑101 (MQ=255)
                                                                                       cggACTGAACCTAAGAA.CGGGGACAGTCGACCAGAAACCCCAAGGAGCACATCACCGCCAAAATGACGGCGGACTGAACCTGCCGGGTCTCATTGAGGATa            <  2:4470697/101‑1 (MQ=255)
                                                                                        ggACTGAACCTAAGAA.CGGGGACAGTCGACCAGAAACCCCAAGGAGCACATCACCGCCAAAATGACGGCGGACTGAACCTGCCGGGTCTCATTGAGGATaa           <  2:1183173/101‑1 (MQ=255)
                                                                                        ggACTGAACCTAAGAA.CGGGGACAGTCGACCAGAAACCCCAAGGAGCACATCACCGCCAAAATGACGGCGGACTGAACCTGCCGGGTCTCATTGAGGATaa           <  2:2113064/101‑1 (MQ=255)
                                                                                        ggACTGAACCTAAGAA.CGGGGACAGTCGACCAGAAACCCCAAGGAGCACATCACCGCCAAAATGACGGCGGACTGAACCTGCCGGGTCTCATTGAGGATaa           >  2:2760254/1‑101 (MQ=255)
                                                                                         gACTGAACCTAAGAA.CGGGGACAGTCGACCAGAAACCCCAAGGAGCACATCACCGCCAAAATGACGGCGGACTGAACCTGCCGGGTCTCATTGAGGATAAg          <  1:4337328/101‑1 (MQ=255)
                                                                                          gcTGAACCTAAGAA.CGGGGCCAGTCAACCAGAAGCCCCAAGGAGCACATCACCGCGAAAATGACGGCGGACTGACCCTGCCGGGTCTCATTGAGGATAAgg         <  2:3455046/100‑1 (MQ=25)
                                                                                          aCTGAACCTAAGAA.CGGGGACAGTCGACCAGAAACCCCAAGGAGCACATCACCGCCAAAATGACGGCGGACTGAACCTGCCGGGTCTCATTGAGGATAAgg         >  2:3742498/1‑101 (MQ=255)
                                                                                            tGAACCTAAGAA.CGGGGACAGTCGACCAGAAACCCCAAGGAGCACATCACCGCCAAAATGACGGCGGACTGAACCTGCCGGGTCTCATTGAGGATAAGGGc       <  2:2984732/101‑1 (MQ=255)
                                                                                             gAACCTAAGAA.CGGGGACAGTCGACCAGAAACCCCAAGGAGCACATCACCGCCAAAATGACGGCGGACTGAACCTGCCGGGTCTCATTGAGGATAAGGGCa      >  2:3501022/1‑101 (MQ=255)
                                                                                             gAACCTAAGAA.CGGGGACAGTCGACCAGAAACCCCAAGGAGCACATCACCGCCAAAATGACGGCGGACTGAACCTGCCGGGTCTCATTGAGGATAAGGGCa      >  2:812964/1‑101 (MQ=255)
                                                                                              aaCCTAAGAA.CGGGGACAGTCGACCAGAAACCCCAAGGAGCACATCACCGCCAAAATGACGGCGGACTGAACCTGCCGGGTCTCATTGAGGATAAGGGCaa     >  1:4163009/1‑101 (MQ=255)
                                                                                              aaCCTAAGAA.CGGGGACAGTCGACCAGAAACCCCAAGGAGCACATCACCGCCAAAATGACGGCGGACTGAACCTGCCGGGTCTCATTGAGGATAAGGGCaa     <  1:20190/101‑1 (MQ=255)
                                                                                              aaCCTAAGAA.CGGGGACAGGCGACCAGAAACCCCAAGGAGCACATCGCCGCCAAAATGACGGCGGACTGAACCTGCCGGGTCTCATTGAGGATAAGGGCaa     <  2:3771496/101‑1 (MQ=37)
                                                                                               aCCTAAGAA.CGGGGACAGTCGACCAGAAACCCCAAGGAGCACATCACCGCCAAAATGACGGCGGACTGAACCTGCCGGGTCTCATTGAGGATAAGGGCAAt    <  2:380044/101‑1 (MQ=255)
                                                                                                ccTAAGAA.CGGGGACAGTCGACCAGAAACCCCAAGGAGCACATCACCGCCAAAATGACGGCGGACTGAACCTGCCGGGTCTCATTGAGGATAAGGGCAATc   <  1:2596500/101‑1 (MQ=255)
                                                                                                 cTAAGAA.CGGGGACAGTCGCCCAGAACACCGAAGGAGCACATCACCGCCAAAATGACGGCGGACTGAACCTGCCGGGTCTCATTGAGGATAAGGGCAATCg  <  1:2804071/101‑1 (MQ=25)
                                                                                                      |                                                                                                
TCGTCACCCTCCGAAACCTCGCCGCGGATCTCGATTGATTTCAGGGCGCTCCAGTGGTAAAGGAGCACATCACCGCCAAAATGACGGCGGACTGAACCTAAG.A.CGGGGACAGTCGACCAGAAACCCCAAGGAGCACATCACCGCCAAAATGACGGCGGACTGAACCTGCCGGGTCTCATTGAGGATAAGGGCAATCG  >  NC_014034/2162376‑2162572

Base quality scores:  ATCG  < 3 ≤  ATCG  < 22 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG