Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,177,270 +AG 100% coding (107/990 nt) rbsB ← ribose ABC transporter periplasmic ribose‑binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,177,2701.→A100.0% 781.4 195coding (107/990 nt)rbsBribose ABC transporter periplasmic ribose‑binding protein
Reads supporting (aligned to +/- strand):  new base (103/92):  ref base (0/0):  total (103/92)
*NC_0140342,177,2702.→G100.0% 748.2 195coding (107/990 nt)rbsBribose ABC transporter periplasmic ribose‑binding protein
Reads supporting (aligned to +/- strand):  new base (103/92):  ref base (0/0):  total (103/92)

Only 100 of 228 total aligned reads displayed.

CTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGGGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAA..GGGT..TG..GT..CGGTCTT.GTGTACAGACAGGCCCCGGTTTCGGCCG.AGCCGCCCCGGCCAGCGCGGAAAACGCCA.GGCAGAGGCCCC  >  NC_014034/2177170‑2177354
                                                                                                     ||                                                                                             
cTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGaaaa                                                                                                 <  1:4294250/101‑1 (MQ=255)
 tGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGaaaaa                                                                                                >  2:1040706/1‑100 (MQ=255)
  gCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGaaaaag                                                                                               >  1:26872/1‑99 (MQ=255)
    gTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAA..aggg                                                                                           <  1:1012316/101‑2 (MQ=255)
    gTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAA..aggg                                                                                           >  1:548504/1‑100 (MQ=255)
     tttCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAA..agggg                                                                                          <  1:433951/101‑3 (MQ=255)
      ttCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAA..aggggt                                                                                         >  2:4285126/1‑98 (MQ=255)
      ttCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAA..aggggt                                                                                         <  2:2775530/101‑4 (MQ=255)
      ttCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAA..aggggt                                                                                         >  2:1372021/1‑98 (MQ=255)
       tCCACATCGCCATCGATCTTGCCCGAATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAA..aggggtt                                                                                        >  2:888827/1‑97 (MQ=255)
        ccACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAA..aggggttg                                                                                       <  1:2109150/101‑6 (MQ=255)
         cacaTCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAA..aggggttgg                                                                                      <  2:91102/101‑7 (MQ=255)
         cacaTCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAA..aggggttgg                                                                                      >  2:4606221/1‑95 (MQ=255)
         cacaTCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAA..aggggttgg                                                                                      >  2:3494976/1‑95 (MQ=255)
           caTCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGACCCTTCCTTCATCTTGACGAAAAAGGGGT..Tg..gtg                                                                                  >  1:4005260/1‑100 (MQ=255)
            aTCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..Tg..gtgt                                                                                 >  2:222138/1‑99 (MQ=255)
              cGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..Tg..gtgtcg                                                                               >  1:640892/1‑97 (MQ=255)
              cGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..Tg..gtgtcg                                                                               >  2:549311/1‑97 (MQ=255)
              cGCCAACGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..Tg..gtgtcg                                                                               <  2:117940/101‑5 (MQ=255)
               gCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..Tg..gtgtcgg                                                                              >  1:1380398/1‑96 (MQ=255)
               gCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..Tg..gtgtcgg                                                                              <  1:687946/101‑6 (MQ=255)
               gCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..Tg..gtgtcgg                                                                              >  1:4452599/1‑96 (MQ=255)
               gCCATCCATCTTGCCCGGATAGGTCAAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..Tg..gtgtcgg                                                                              <  2:2272324/101‑6 (MQ=255)
                ccATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..Tg..gtgtcggt                                                                             <  2:3191642/101‑7 (MQ=255)
                ccATCGATATTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCAAAGTATCTTCCTTCATCTTGACGAAAAAGGGGT..Tg..gtgtcggt                                                                             >  2:3849361/1‑95 (MQ=255)
                 cATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..Tg..gtgtcggtc                                                                            >  1:3682720/1‑94 (MQ=255)
                   tCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..Tg..gtgtcggtctt                                                                          >  2:3475521/1‑92 (MQ=255)
                    cGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTGT..CGGTCTt.g                                                                        >  1:331165/1‑101 (MQ=255)
                         ttGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTGT..CGGTCTt.ggtgat                                                                   >  1:3523979/1‑96 (MQ=255)
                           gCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTGT..CGGTCTt.ggtgatca                                                                 <  1:1125871/101‑8 (MQ=255)
                           gCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTGT..CGGTCTt.ggtgatca                                                                 <  1:3172036/101‑8 (MQ=255)
                            cccGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTGT..CGGTCTt.ggtgatcag                                                                <  2:857954/101‑9 (MQ=255)
                            cccGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTGT..CGGTCTt.ggtgatcag                                                                <  2:639702/101‑9 (MQ=255)
                            cccGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTGT..CGGTCTt.ggtgatcag                                                                <  1:3085174/101‑9 (MQ=255)
                             ccGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTGT..CGGTCTt.ggtgatcaga                                                               <  2:4413919/101‑10 (MQ=255)
                              cGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTGT..CGGTCTt.ggtgatcagac                                                              >  1:107639/1‑91 (MQ=255)
                              cGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTGT..CGGTCTt.ggtgatcagac                                                              <  2:2974143/101‑11 (MQ=255)
                              cGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTGT..CGGTCTt.ggtgatcagac                                                              >  1:3210523/1‑91 (MQ=255)
                                cATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTGT..CGGTCTTGGTGAtcagacag                                                             >  1:2456289/1‑101 (MQ=255)
                                cATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTGT..CGGTCTTGGTGAtcagacag                                                             >  2:2435653/1‑101 (MQ=255)
                                 aTAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTGT..CGGTCTTGGTGATCAGACAgg                                                            >  2:1940203/1‑101 (MQ=255)
                                 aTAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTGT..CGGTCTTGGTGATCAGACAgg                                                            >  1:3565271/1‑101 (MQ=255)
                                     gTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTGT..CGGTCTTGGTGATCAGACAGGcccc                                                        >  1:3625745/1‑101 (MQ=255)
                                      gggAGAGCACCCGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTGT..CGGTCTTGGTGATCAGACAGGCCCCg                                                       <  2:1397285/99‑1 (MQ=255)
                                        gAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGt                                                     >  2:1288617/1‑101 (MQ=255)
                                          gagCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTGT..TGGTCTTGGTGATCAGACTGGCCCCGGttt                                                   <  2:2039335/99‑1 (MQ=255)
                                          gagCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGttt                                                   <  2:2490345/99‑1 (MQ=255)
                                          gagCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGttt                                                   >  1:1344486/3‑101 (MQ=255)
                                          gagCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGttt                                                   <  2:3523721/99‑1 (MQ=255)
                                            gCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCg                                                 <  1:2376930/101‑1 (MQ=255)
                                             cACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCgg                                                <  1:4501086/101‑1 (MQ=255)
                                             cACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCgg                                                >  2:407594/1‑101 (MQ=255)
                                              aCCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGc                                               <  2:2257341/101‑1 (MQ=255)
                                                cAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCg                                             <  2:580900/101‑1 (MQ=255)
                                                 aGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCG.a                                           <  1:222162/101‑1 (MQ=255)
                                                  tccccATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCG.aa                                          <  1:3290364/100‑2 (MQ=255)
                                                     ccATCTCGGTCGCCTTTGCCGAAGCTCCTTCCTTCATCTTGACGAAAAAGGGGTTGTG..GT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCG.aagcc                                       <  2:1311785/101‑5 (MQ=255)
                                                     ccATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCG.aagcc                                       <  1:2435819/101‑5 (MQ=255)
                                                     ccATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCG.aagcc                                       <  2:3552656/101‑5 (MQ=255)
                                                       aTCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAgccgc                                      <  1:4202292/101‑1 (MQ=255)
                                                        tctcGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAgccgcc                                     >  1:1535170/1‑101 (MQ=255)
                                                         ctcGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGccc                                    >  2:3317274/1‑101 (MQ=255)
                                                          tcGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGcccc                                   >  1:332814/1‑101 (MQ=255)
                                                          tcGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGcccc                                   <  1:3505652/101‑1 (MQ=255)
                                                           cGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCg                                  <  2:3503481/101‑1 (MQ=255)
                                                            ggTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCgg                                 >  2:3601073/1‑101 (MQ=255)
                                                               cGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGccg                              >  2:4156338/1‑100 (MQ=255)
                                                               cGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCa                              <  2:2623498/101‑1 (MQ=255)
                                                                gCCTTTTCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAg                             <  1:775533/101‑1 (MQ=255)
                                                                gCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAg                             <  1:4117049/101‑1 (MQ=255)
                                                                 ccTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAgc                            >  1:2876285/1‑101 (MQ=255)
                                                                 ccTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAgc                            <  1:1870166/101‑1 (MQ=255)
                                                                 ccTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAgc                            <  2:3971190/101‑1 (MQ=255)
                                                                  cTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAgcg                           <  2:4548891/101‑1 (MQ=255)
                                                                    ttGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGcgcg                         >  2:781874/1‑101 (MQ=255)
                                                                     tGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCgg                        >  1:1616766/1‑101 (MQ=255)
                                                                       ccGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGaa                      >  2:1506157/1‑101 (MQ=255)
                                                                       ccGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGaa                      >  2:1671003/1‑101 (MQ=255)
                                                                       ccGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGaa                      <  2:204425/101‑1 (MQ=255)
                                                                       ccGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGaa                      >  2:2388531/1‑101 (MQ=255)
                                                                       ccGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTGT..CGGTCTTGGTCATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGaa                      >  1:1572506/1‑101 (MQ=255)
                                                                        cGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGaaa                     >  1:4348612/1‑101 (MQ=255)
                                                                         gAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTGT..CGGTCTTGGTGATCAGACCGGCCCCGGCTTCGGCCGAAGCCGGCCCGGCCAGCGCCGacaa                    >  1:128843/1‑101 (MQ=255)
                                                                           aggaCCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACg                  <  1:3849646/98‑1 (MQ=255)
                                                                             cACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGcc                <  1:3480781/101‑1 (MQ=255)
                                                                               ccttccttCATCTTGACGAAAAAGGGGT..TGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.g             <  2:1535063/101‑1 (MQ=255)
                                                                               ccttccttCATCTTGACGAAAAAGGGGT..TGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.g             <  1:486626/101‑1 (MQ=255)
                                                                                cttccttcATCTTGGCGAAAAAGGGGT..TGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gg            <  1:2145410/101‑1 (MQ=255)
                                                                                cttccttcATCTTGACGAAAAAGGGGT..TGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gg            >  2:4638462/1‑101 (MQ=255)
                                                                                 ttccttcATCTTGACGAAAAAGGGGT..TGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.ggg           >  1:951329/1‑100 (MQ=255)
                                                                                 ttccttcATCTTGACGAAAAAGGGGT..TGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.ggg           >  1:4223900/1‑100 (MQ=255)
                                                                                  tccttcATCTTGACGAAAAAGGGGT..TGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggc          >  1:2971033/1‑99 (MQ=255)
                                                                                    cttcATCTTGACGAAAAAGGGGT..TGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggcag        <  1:4601486/101‑5 (MQ=255)
                                                                                     ttcATCTTGACGAAAAAGGGGT..TGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggcaga       >  1:1039340/1‑96 (MQ=255)
                                                                                     ttcATCTTGACGAAAAAGGGGT..TGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggcaag       >  1:3373636/1‑96 (MQ=255)
                                                                                       cATCTTGACGAAAAAGGGGT..TGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAgg      >  1:1273251/1‑101 (MQ=255)
                                                                                        aTCTTGACGAAAAAGGGGT..TGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCaagatcggaag     >  2:3382245/1‑91 (MQ=255)
                                                                                         tCTTGACGAAAAAGGGGT..TGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGcc    <  1:2808819/101‑1 (MQ=255)
                                                                                         tCTTGACGAAAAAGGGGT..TG..GTGGCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGcc    >  2:1827423/1‑101 (MQ=255)
                                                                                           ttGACGAAAAAGGGGT..TGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGcacc  >  2:3933967/1‑101 (MQ=255)
                                                                                                     ||                                                                                             
CTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGGGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAA..GGGT..TG..GT..CGGTCTT.GTGTACAGACAGGCCCCGGTTTCGGCCG.AGCCGCCCCGGCCAGCGCGGAAAACGCCA.GGCAGAGGCCCC  >  NC_014034/2177170‑2177354

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG