Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,177,342 +G 100% coding (35/990 nt) rbsB ← ribose ABC transporter periplasmic ribose‑binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,177,3421.→G100.0% 662.5 174coding (35/990 nt)rbsBribose ABC transporter periplasmic ribose‑binding protein
Reads supporting (aligned to +/- strand):  new base (80/94):  ref base (0/0):  total (80/94)

Only 100 of 196 total aligned reads displayed.

ACCTTCCTTCATCTTGACGAAAA..GGGTTG..GTCGGTCTT.GTGTA.CAGACAGGCCCCGGTTTCGGCC.G.AGCCGCCCCGGCCAGCGCGGAAAACGCCA.GGCAGAGG.CC.CCAACAGGGCCA.G.TTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGA  >  NC_014034/2177248‑2177435
                                                                                                       |                                                                                                 
aCCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCa                                                                                                    <  2:535089/101‑1 (MQ=255)
   ttccttcATCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.ggg                                                                                                >  1:951329/1‑100 (MQ=255)
   ttccttcATCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.ggg                                                                                                >  1:4223900/1‑100 (MQ=255)
    tccttcATCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggc                                                                                               >  2:3989549/1‑99 (MQ=255)
     ctttcATCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggca                                                                                              <  2:3373386/99‑4 (MQ=255)
      cttcATCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggcag                                                                                             <  1:4601486/101‑5 (MQ=255)
      cttcATCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggcag                                                                                             >  1:3025176/1‑97 (MQ=255)
       ttcATCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggcaga                                                                                            >  1:1039340/1‑96 (MQ=255)
       ttcATCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggcaga                                                                                            >  1:434106/1‑96 (MQ=255)
       ttcATCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggcaag                                                                                            >  1:3373636/1‑96 (MQ=255)
        tcATCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCagag                                                                                            >  1:4445006/1‑101 (MQ=255)
        tcATCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCagag                                                                                            >  2:484180/1‑101 (MQ=255)
         cATCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAgg                                                                                           >  2:2474680/1‑101 (MQ=255)
         cATCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAgg                                                                                           >  1:1309149/1‑101 (MQ=255)
         cATCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAgg                                                                                           <  1:987794/101‑1 (MQ=255)
         cATCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAgg                                                                                           >  1:1273251/1‑101 (MQ=255)
          aTCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCaagatcggaag                                                                                          >  2:3382245/1‑91 (MQ=255)
          aTCTTGACGAAAAAGGGGTTGGTGTCGGTCTTCGTGAT.CAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGG.c                                                                                         >  1:2556549/1‑101 (MQ=255)
            cTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGG.cc.c                                                                                      >  2:4497451/1‑101 (MQ=255)
            cTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGG.cc.c                                                                                      >  1:2881723/1‑101 (MQ=255)
             ttGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGG.cc.cc                                                                                     <  1:3265855/101‑1 (MQ=255)
             ttGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGG.ca.cc                                                                                     >  2:3933967/1‑101 (MQ=255)
                           gttggtgtcggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.G.tt                                                                      <  1:471822/95‑1 (MQ=255)
                            ttggtgtcggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.G.ttt                                                                     <  1:2390646/96‑2 (MQ=255)
                             tggtgtcggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.G.tttg                                                                    <  2:1039257/97‑3 (MQ=255)
                             tggtgtcggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.G.tttg                                                                    <  2:1564048/97‑3 (MQ=255)
                              ggtgtcggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.G.tttgc                                                                   <  1:506834/98‑4 (MQ=255)
                              ggtgtcggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.G.tttgc                                                                   >  2:2731979/4‑98 (MQ=255)
                              ggtgtcggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.G.tttgc                                                                   >  2:3195176/4‑98 (MQ=255)
                               gtgtcggtcTTGGTGAT.CAGACAGGCCCCGNTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.G.tttgcg                                                                  <  1:19746/99‑5 (MQ=255)
                               gtgtcggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.G.tttgcg                                                                  <  1:716198/99‑5 (MQ=255)
                                tgtcggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.G.tttgcgc                                                                 >  2:667951/2‑96 (MQ=255)
                                tgtcggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.G.tttgcgc                                                                 >  1:1645370/2‑96 (MQ=255)
                                tgtcggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.G.tttgcgc                                                                 >  2:356407/2‑96 (MQ=255)
                                  tcggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGcat                                                                <  1:1809581/101‑1 (MQ=255)
                                  gcggtcTT.GGGCACCAGACAGGCCCAGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGcat                                                                <  2:257263/100‑1 (MQ=255)
                                   cggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGcatc                                                               <  2:3051331/101‑1 (MQ=255)
                                   cggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGcatc                                                               >  2:1157850/1‑101 (MQ=255)
                                       tcttggtgatcagacagGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCatcatc                                                            <  1:1885137/91‑1 (MQ=255)
                                       tcttggtgatcagacagGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCatcatc                                                            >  1:1523453/11‑101 (MQ=255)
                                        cttggtgatcagacagGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACCGGGCCA.GTTTGCGCAtcatca                                                           <  1:2643655/92‑1 (MQ=255)
                                        cttggtgatcagacagGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCAtcatca                                                           <  1:2042882/92‑1 (MQ=255)
                                         ttggtgatcagacagGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCAc                                                          >  2:1163267/9‑101 (MQ=255)
                                         ttggtgatcagacagGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCAc                                                          <  2:196968/93‑1 (MQ=255)
                                          tggtgatcagacagGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACg                                                         <  2:548456/94‑1 (MQ=255)
                                          tggtgatcagacagGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACg                                                         <  1:888903/94‑1 (MQ=255)
                                            gtgatcagacagGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGt                                                       <  2:4444711/96‑1 (MQ=255)
                                              gatcagacagGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGtct                                                     >  1:4419914/4‑101 (MQ=255)
                                              gatcagacagGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGtct                                                     >  1:802006/4‑101 (MQ=255)
                                              gatcagacagGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACACCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGtct                                                     <  1:2952298/98‑1 (MQ=255)
                                               atcagacagGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGtctc                                                    <  2:325213/99‑1 (MQ=255)
                                                  agacagGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTctcc                                                 <  1:2698009/101‑1 (MQ=255)
                                                     cagGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCtcctcc                                              <  1:1040789/101‑1 (MQ=255)
                                                      agGCCGCGGTTTCGGCCTA.AGCCGACCCGGCCAGCCCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTccc                                             <  2:2627088/101‑1 (MQ=255)
                                                      agGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTccc                                             >  2:2387636/1‑101 (MQ=255)
                                                      agGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTccc                                             >  1:4494304/1‑101 (MQ=255)
                                                      agGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTccc                                             <  1:3191311/101‑1 (MQ=255)
                                                       ggCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCg                                            >  1:258898/1‑101 (MQ=255)
                                                       ggCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCg                                            >  1:1585748/1‑101 (MQ=255)
                                                          cccGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGttt                                         >  1:2830317/1‑101 (MQ=255)
                                                           ccGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTc                                        <  2:801932/101‑1 (MQ=255)
                                                           ccGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTc                                        >  2:1915471/1‑101 (MQ=255)
                                                            cGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCGCCTCCCGTTTCa                                       <  1:2528363/101‑1 (MQ=255)
                                                              gTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACt                                     >  1:1163766/1‑101 (MQ=255)
                                                              gTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACt                                     <  1:2474846/101‑1 (MQ=255)
                                                                ttCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGt                                   <  1:3475779/101‑1 (MQ=255)
                                                                ttCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGt                                   <  2:3433570/101‑1 (MQ=255)
                                                                 tCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTc                                  >  1:373957/1‑101 (MQ=255)
                                                                 tCGGCC.GAAGCCGCCCCGGCCAGCGCCGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCTA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTc                                  <  2:1512559/101‑1 (MQ=255)
                                                                      ccgaaGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGcc                              <  1:2353818/97‑1 (MQ=255)
                                                                       cgaaGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGccc                             <  2:4348320/98‑1 (MQ=255)
                                                                        gaaGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGcccc                            >  1:65126/3‑101 (MQ=255)
                                                                        gaaGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCAACACGGTCTCTCCTCCCGTTTCACTGTCGGcccc                            >  1:4019649/3‑101 (MQ=255)
                                                                         aaGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCcct                           >  2:4169122/2‑101 (MQ=255)
                                                                          aGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCcctc                          <  1:1671114/101‑1 (MQ=255)
                                                                            ccgccCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCcctcct                        >  1:393777/1‑101 (MQ=255)
                                                                             cgccCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCctcctc                       <  1:2121694/101‑1 (MQ=255)
                                                                              gccCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCtcctcc                      >  2:3551534/1‑101 (MQ=255)
                                                                              gccCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCtcctcc                      <  1:2165111/101‑1 (MQ=255)
                                                                              gccCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCtcctcc                      >  2:1715251/1‑101 (MQ=255)
                                                                                cccGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCgg                    >  2:4583195/1‑101 (MQ=255)
                                                                                  cGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCgggg                  <  2:3792051/101‑1 (MQ=255)
                                                                                    gCCAGCGCGGAAAACGCCAGGGCAGAGG.CCNCCAACAGGGCCAGG.TTGCGCATCATCACGGGCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCt                <  1:2276419/101‑1 (MQ=255)
                                                                                     ccAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTc               <  2:2434735/101‑1 (MQ=255)
                                                                                       aGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCgg             <  1:3390525/101‑1 (MQ=255)
                                                                                         cgcgGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGAc           <  2:3682445/101‑1 (MQ=255)
                                                                                         cgcgGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGAc           >  1:3618482/1‑101 (MQ=255)
                                                                                          gcgGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACa          >  2:3492337/1‑101 (MQ=255)
                                                                                            ggAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACaaa        <  2:3177969/101‑1 (MQ=255)
                                                                                             gAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCAGCATCACGGTCTCTCCTCCCATTACACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAg       >  1:3987099/1‑101 (MQ=255)
                                                                                              aaaaCGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGt      <  2:1376775/101‑1 (MQ=255)
                                                                                              aaaaCGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGt      >  1:3568172/1‑101 (MQ=255)
                                                                                               aaaCGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTc     >  2:4421483/1‑101 (MQ=255)
                                                                                               aaaCGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTc     >  2:3112280/1‑101 (MQ=255)
                                                                                                 aCGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTg   <  2:3617003/101‑1 (MQ=255)
                                                                                                 aCGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTg   >  1:3059741/1‑101 (MQ=255)
                                                                                                 aCGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTg   <  2:2140861/101‑1 (MQ=255)
                                                                                                  cGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGa  >  1:610261/1‑101 (MQ=255)
                                                                                                  cGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGa  <  2:1282643/101‑1 (MQ=255)
                                                                                                  cGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGa  >  2:2306702/1‑101 (MQ=255)
                                                                                                       |                                                                                                 
ACCTTCCTTCATCTTGACGAAAA..GGGTTG..GTCGGTCTT.GTGTA.CAGACAGGCCCCGGTTTCGGCC.G.AGCCGCCCCGGCCAGCGCGGAAAACGCCA.GGCAGAGG.CC.CCAACAGGGCCA.G.TTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGA  >  NC_014034/2177248‑2177435

Base quality scores:  ATCG  < 3 ≤  ATCG  < 24 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG