Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,177,350 +C 100% coding (27/990 nt) rbsB ← ribose ABC transporter periplasmic ribose‑binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,177,3501.→C100.0% 690.5 182coding (27/990 nt)rbsBribose ABC transporter periplasmic ribose‑binding protein
Reads supporting (aligned to +/- strand):  new base (81/100):  ref base (0/1):  total (81/101)

Only 100 of 194 total aligned reads displayed.

CATCTTGACGAAAA..GGGTTG..GT..CGGTCTT.GTGTACAGACAGGCCCCGGTTTCGGCC.G.AGCCGCCCCGGCCAGCGCGGAAAACGCCA.GGCAGAGG.CC.CCAACAGGGCCA.G.TTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTCG  >  NC_014034/2177257‑2177444
                                                                                                        |                                                                                                 
cATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAgg                                                                                                    >  1:1309149/1‑101 (MQ=255)
cATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAgg                                                                                                    >  2:2474680/1‑101 (MQ=255)
  tCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGG.cc                                                                                                 <  1:2808819/101‑1 (MQ=255)
  tCTTGACGAAAAAGGGGTTG..GTGGCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGG.cc                                                                                                 >  2:1827423/1‑101 (MQ=255)
    ttGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGG.cc.cc                                                                                              <  1:3265855/101‑1 (MQ=255)
    ttGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGG.ca.cc                                                                                              >  2:3933967/1‑101 (MQ=255)
               ggggttggtgt..cggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCa                                                                                    >  2:3661406/10‑101 (MQ=255)
                gggttggtgt..cggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.g                                                                                  <  2:3531409/93‑1 (MQ=255)
                  gttggtgt..cggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.G.tt                                                                               <  1:471822/95‑1 (MQ=255)
                  gttggtgt..cggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.G.tt                                                                               <  2:1949770/95‑1 (MQ=255)
                   ttggtgt..cggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.G.ttt                                                                              <  1:620353/96‑2 (MQ=255)
                    tggtgt..cggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.G.tttg                                                                             >  1:2588294/5‑99 (MQ=255)
                     ggtgt..cggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.G.tttgc                                                                            >  2:2731979/4‑98 (MQ=255)
                     ggtgt..cggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.G.tttgc                                                                            >  2:2532896/4‑98 (MQ=255)
                      gtgt..cggtcTTGGTGATCAGACAGGCCCCGNTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.G.tttgcg                                                                           <  1:19746/99‑5 (MQ=255)
                       tgt..cggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.G.tttgcgc                                                                          >  2:667951/2‑96 (MQ=255)
                       tgt..cggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.G.tttgcgc                                                                          >  2:1024315/2‑96 (MQ=255)
                               tcttggtgatcagacagGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCatcatc                                                                     <  1:1885137/91‑1 (MQ=255)
                               tcttggtgatcagacagGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCatcatc                                                                     <  2:1223441/91‑1 (MQ=255)
                                cttggtgatcagacagGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCAtcatca                                                                    <  1:2042882/92‑1 (MQ=255)
                                 ttggtgatcagacagGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCAc                                                                   <  2:4429127/93‑1 (MQ=255)
                                 ttggtgatcagacagGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCAc                                                                   >  1:3492412/9‑101 (MQ=255)
                                  tggtgatcagacagGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACg                                                                  <  1:888903/94‑1 (MQ=255)
                                  tggtgatcagacagGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACg                                                                  <  1:2859812/94‑1 (MQ=255)
                                      gatcagacagGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGtct                                                              >  1:2697068/4‑101 (MQ=255)
                                      gatcagacagGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACACCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGtct                                                              <  1:2952298/98‑1 (MQ=255)
                                       atcagacagGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGtctc                                                             >  2:2385478/3‑101 (MQ=255)
                                       atcagacagGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGtctc                                                             <  2:325213/99‑1 (MQ=255)
                                         cagacggGCCCCGGTTGCGGCC.GAAGCCGCCCTGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGtctctc                                                           <  2:3709008/101‑1 (MQ=255)
                                         cagacagGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGtctctc                                                           <  1:1723243/101‑1 (MQ=255)
                                         cagacagGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GATTGCGCATCATCACGGtctctc                                                           >  1:958063/1‑101 (MQ=255)
                                           gacagGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTctcct                                                         <  2:3375109/101‑1 (MQ=255)
                                             cagGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCtcctcc                                                       >  1:2633451/1‑101 (MQ=255)
                                             cagGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCtcctcc                                                       <  1:1040789/101‑1 (MQ=255)
                                              agGCCGCGGTTTCGGCCTA.AGCCGACCCGGCCAGCCCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTccc                                                      <  2:2627088/101‑1 (MQ=255)
                                              agGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTccc                                                      <  1:3191311/101‑1 (MQ=255)
                                              agGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTccc                                                      >  2:1063658/1‑101 (MQ=255)
                                              agGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTccc                                                      <  1:2431225/101‑1 (MQ=255)
                                              agGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTccc                                                      >  1:4494304/1‑101 (MQ=255)
                                              agGCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACCGTCTCTCCTccc                                                      <  1:2533070/101‑1 (MQ=255)
                                               ggCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCg                                                     >  1:1585748/1‑101 (MQ=255)
                                                gCCCCGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGt                                                    <  1:434313/101‑1 (MQ=255)
                                                 ccccGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGtt                                                   <  2:3597170/101‑1 (MQ=255)
                                                  cccGGTGTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGttt                                                  <  1:1816250/101‑1 (MQ=255)
                                                   ccGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTc                                                 <  2:801932/101‑1 (MQ=255)
                                                    cGGTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCa                                                <  2:4452304/101‑1 (MQ=255)
                                                      gTTTCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACt                                              >  1:1163766/1‑101 (MQ=255)
                                                        ttCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGt                                            <  2:3433570/101‑1 (MQ=255)
                                                        ttCGGCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGt                                            >  1:165039/1‑101 (MQ=255)
                                                         tCGGCC.GAAGCCGCCCCGGCCAGCGCCGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCTA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTc                                           <  2:1512559/101‑1 (MQ=255)
                                                           ggCC.GAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCgg                                         >  1:228067/1‑101 (MQ=255)
                                                               cgaaGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGccc                                      >  1:4532784/4‑101 (MQ=255)
                                                               cgaaGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGccc                                      <  1:2501571/98‑1 (MQ=255)
                                                               cgaaGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGccc                                      <  2:4348320/98‑1 (MQ=255)
                                                                gaaGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGcccc                                     <  2:3556452/99‑1 (MQ=255)
                                                                gaaGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGcccc                                     >  2:4510321/3‑101 (MQ=255)
                                                                gaaGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCAACACGGTCTCTCCTCCCGTTTCACTGTCGGcccc                                     >  1:4019649/3‑101 (MQ=255)
                                                                 aaGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCcct                                    >  2:4169122/2‑101 (MQ=255)
                                                                     cgccCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCctcctc                                <  2:2588120/101‑1 (MQ=255)
                                                                     cgccCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCctcctc                                <  2:1616661/101‑1 (MQ=255)
                                                                     cgccCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCctcctc                                <  1:2121694/101‑1 (MQ=255)
                                                                     cgccCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCctcctc                                <  1:840579/101‑1 (MQ=255)
                                                                     cgccCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCctcctc                                <  1:668013/101‑1 (MQ=255)
                                                                      gccCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCtcctcc                               <  1:1529945/101‑1 (MQ=255)
                                                                      gccCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCtcctcc                               <  2:1160606/101‑1 (MQ=255)
                                                                      gccCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCtcctcc                               >  1:4443226/1‑101 (MQ=255)
                                                                       ccccGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCg                              <  1:2166542/101‑1 (MQ=255)
                                                                        cccGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCgg                             >  1:4176096/1‑101 (MQ=255)
                                                                          cGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCgggg                           <  2:3792051/101‑1 (MQ=255)
                                                                          cGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCgggg                           <  1:3934252/101‑1 (MQ=255)
                                                                            gCCAGCGCGGAAAACGCCAGGGCAGAGG.CCNCCAACAGGGCCAGG.TTGCGCATCATCACGGGCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCt                         <  1:2276419/101‑1 (MQ=255)
                                                                             ccAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTc                        <  2:2434735/101‑1 (MQ=255)
                                                                              cAGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCg                       >  2:2873165/1‑101 (MQ=255)
                                                                               aGCGCGGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCgg                      <  1:3390525/101‑1 (MQ=255)
                                                                                 cgcgGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGAc                    >  1:3618482/1‑101 (MQ=255)
                                                                                  gcgGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACa                   >  2:3492337/1‑101 (MQ=255)
                                                                                   cgGAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTAGGACaa                  <  1:2387798/101‑1 (MQ=255)
                                                                                    ggAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACaaa                 <  2:3177969/101‑1 (MQ=255)
                                                                                    ggAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACaaa                 <  2:3051855/101‑1 (MQ=255)
                                                                                    ggAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACaaa                 <  2:2178170/101‑1 (MQ=255)
                                                                                    ggAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACaaa                 >  2:547554/1‑101 (MQ=255)
                                                                                     gAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAg                >  1:582447/1‑101 (MQ=255)
                                                                                     gAAAACGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCAGCATCACGGTCTCTCCTCCCATTACACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAg                >  1:3987099/1‑101 (MQ=255)
                                                                                      aaaaCGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGt               >  1:3568172/1‑101 (MQ=255)
                                                                                      aaaaCGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGt               <  2:1376775/101‑1 (MQ=255)
                                                                                       aaaCGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTc              >  2:3112280/1‑101 (MQ=255)
                                                                                         aCGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTg            >  1:3059741/1‑101 (MQ=255)
                                                                                         aCGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTg            <  2:2140861/101‑1 (MQ=255)
                                                                                          cGCCAGGGCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGa           >  2:2306702/1‑101 (MQ=255)
                                                                                            ccagggCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGAcc         <  1:1206373/97‑1 (MQ=255)
                                                                                             cagggCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGAccc        >  2:3210175/4‑101 (MQ=255)
                                                                                              agggCAGCGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGCCTGACCCg       >  1:3710162/3‑101 (MQ=255)
                                                                                              agggCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCg       <  1:3807649/99‑1 (MQ=255)
                                                                                              agggCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCg       >  1:741670/3‑101 (MQ=255)
                                                                                                ggCAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAg     <  1:1024398/101‑1 (MQ=255)
                                                                                                  cAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTc   <  2:1332692/101‑1 (MQ=255)
                                                                                                  cAGAGGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTc   >  2:4658458/1‑101 (MQ=255)
                                                                                                   agagGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTCg  >  2:2397065/1‑101 (MQ=255)
                                                                                                   agagGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTCg  <  2:2100761/101‑1 (MQ=255)
                                                                                                   agagGCCC.CCAACAGGGCCA.GTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTCg  >  1:1626312/1‑101 (MQ=255)
                                                                                                        |                                                                                                 
CATCTTGACGAAAA..GGGTTG..GT..CGGTCTT.GTGTACAGACAGGCCCCGGTTTCGGCC.G.AGCCGCCCCGGCCAGCGCGGAAAACGCCA.GGCAGAGG.CC.CCAACAGGGCCA.G.TTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTCG  >  NC_014034/2177257‑2177444

Base quality scores:  ATCG  < 3 ≤  ATCG  < 22 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG