Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,180,161 +C 100% coding (850/1314 nt) RCAP_rcc02022 → polyols ABC transporter periplasmic polyols‑binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,180,1611.→C100.0% 659.9 177coding (850/1314 nt)RCAP_rcc02022polyols ABC transporter periplasmic polyols‑binding protein
Reads supporting (aligned to +/- strand):  new base (82/94):  ref base (1/0):  total (83/94)

Only 100 of 205 total aligned reads displayed.

CTTCCAGCAGGGCCATTGCGGCATGTGGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCG.GCTGGC.CCGAAC.AAG.G.CGGTGTCCGCAAGCATGGCAACTGGCTCTGGGCCTGGAACCCTCGCCAATTCCGGCGGGCACGCAAAAAGGCCGAGGCG  >  NC_014034/2180061‑2180256
                                                                                                     |                                                                                                   
ctTCCAGCAGGGCCATTGCGGCATGTGGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGtcggcttcg                                                                                                      <  2:4586521/101‑1 (MQ=255)
 ttCCAGCAGGGCCATTGCGGCATGTGGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGtcggcttcgc                                                                                                     <  1:3772059/101‑2 (MQ=255)
   ccAGCAGGGCCATTGCGGCATGTGGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGtcggcttcgcgc                                                                                                   <  1:2824461/101‑4 (MQ=255)
    cagcagGGCCATTGCGGCATGTGGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGtcggcttcgcgct                                                                                                  >  1:3685290/1‑97 (MQ=255)
    cagcagGGCCATTGCGGCATGTGGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGtcggcttcgcgct                                                                                                  >  2:3625986/1‑97 (MQ=255)
     agcagGGCCATTGCGGCATGTGGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGtcggcttcgcgctg                                                                                                 <  1:1227515/101‑6 (MQ=255)
     agcagGGCCATTGCGGCATGTGGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGtcggcttcgcgctg                                                                                                 >  2:3047598/1‑96 (MQ=255)
      gcagGGCCATTGCGGCATGTGGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTgg                                                                                                <  1:3619862/101‑1 (MQ=255)
       cagGGCCATTGCGGCATGTGGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGc                                                                                               >  2:1561578/1‑101 (MQ=255)
        agGGCCATTGCGGCATGTGGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGcg                                                                                              >  2:24444/1‑100 (MQ=255)
         gggCCATTGCGGCATGTGGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGcgc                                                                                             >  1:776928/1‑99 (MQ=255)
              aTTGCGGCATGTGGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGGCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAc                                                                                        >  1:3940807/1‑101 (MQ=255)
              aTTGCGGCATGTGGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAc                                                                                        <  1:1047015/101‑1 (MQ=255)
              aTTGCGGCATGTGGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAc                                                                                        <  2:2729088/101‑1 (MQ=255)
              aTTGCGGCATGTGGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAc                                                                                        >  1:363801/1‑101 (MQ=255)
               ttGCGGCATGTGGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.a                                                                                      <  2:231244/101‑1 (MQ=255)
                tGCGGCATGTGGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.aa                                                                                     <  1:1052903/101‑1 (MQ=255)
                tGCGGCATGTGGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.aa                                                                                     <  1:2507625/101‑1 (MQ=255)
                 gCGGCATGTGGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAg                                                                                    >  1:3905270/1‑101 (MQ=255)
                 gCGGCATGTGGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAg                                                                                    <  1:530380/101‑1 (MQ=255)
                   ggCATGTGGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGTCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAG.G.c                                                                                >  2:3868891/1‑101 (MQ=255)
                   ggCATGTGGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAG.G.c                                                                                <  1:3811512/101‑1 (MQ=255)
                    gCATGTGGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAG.G.cc                                                                               >  2:299829/1‑100 (MQ=255)
                       tgtgGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAG.G.ccggt                                                                            <  1:2681396/101‑5 (MQ=255)
                       tgtgGAGCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAG.G.ccggt                                                                            <  1:4430785/101‑5 (MQ=255)
                         tgGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGCGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAG.G.ccggggt                                                                          >  1:3722433/1‑95 (MQ=255)
                          ggATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAAG.C.CGgtgtg                                                                          >  2:1380006/1‑100 (MQ=255)
                           gATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAG.GCCGGTGTcg                                                                         <  1:4527511/101‑2 (MQ=255)
                              cGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAG.GCCGGTGTcgcca                                                                      <  1:24449/101‑5 (MQ=255)
                                aTGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAG.GCCGGTGTCGCCAAg                                                                    <  2:1952431/101‑1 (MQ=255)
                                aTGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAG.GCCGGTGTCGCCAAg                                                                    <  1:975704/101‑1 (MQ=255)
                                   cGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAG.GCCGGTGTCGCCAAGCAt                                                                 <  1:2550459/101‑1 (MQ=255)
                                    gACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAG.GCCGGTGTCGCCCAGCATg                                                                >  1:2960223/1‑101 (MQ=255)
                                    gACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAG.GCCGGTGTCGCCAAGCATg                                                                <  1:1510445/101‑1 (MQ=255)
                                    gACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCCGGCGCCGAAC.AAG.GCCGGTGGCGCCAAGCATg                                                                >  1:556369/1‑101 (MQ=255)
                                         tGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAG.GCCGGTGTCGCCAAGCATGGCAAc                                                           >  1:3548042/1‑101 (MQ=255)
                                          ggCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAG.GCCGGTGTCGCCAAGCATGGCAACt                                                          <  1:2237335/101‑1 (MQ=255)
                                           gccgccTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAG.GCCGGTGTCGCCAAGCATGGCAACTg                                                         <  2:13138/101‑1 (MQ=255)
                                           gccgccTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAG.GCCGGTGTCGCCAAGCATGGCAACTg                                                         >  1:3882798/1‑101 (MQ=255)
                                           gcccgctctTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAGAC.CGGTGTCGCCAAGCATGGCAACTg                                                         <  1:4589374/96‑1 (MQ=255)
                                            ccgccTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAG.GCCGGTGTCGCCAAGCATGGCAACTgg                                                        <  2:893810/101‑1 (MQ=255)
                                              gccTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAG.GCCGGTGTCGCCAAGCATGGCAACTGGct                                                      >  2:2974820/1‑101 (MQ=255)
                                              gccTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAG.GCCGGTGTCGCCAAGCATGGCAACTGGct                                                      >  1:862823/1‑101 (MQ=255)
                                                ctctTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGATTCGCGCTGGCGCCGAAC.AAG.GCCGGTGTCGCCAAGCATGGCAACTGGctct                                                    >  2:3372125/1‑101 (MQ=255)
                                                ctatttCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAG.GCCGGTGTCGCCAAGCATGGCAACTGGctct                                                    <  2:3929736/98‑1 (MQ=255)
                                                 tctTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAG.GCCGGTGTCGCCAAGCATGGCAACTGGCTCTg                                                   <  1:139785/101‑1 (MQ=255)
                                                 tctTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAG.GCCGGTGTCGCCAAGCATGGCAACTGGCTCTg                                                   <  2:291379/101‑1 (MQ=255)
                                                  ctTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAG.GCCGGTGTCGCCAAGCATGGCAACTGGCTCTgg                                                  >  2:4088544/1‑101 (MQ=255)
                                                  ctTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAG.GCCGGTGTCGCCAAGCATGGCAACTGGCTCTgg                                                  >  1:3789858/1‑101 (MQ=255)
                                                   tttCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAG.GCCGGTGTCGCCAAGCATGGCAACTGGCTCTggg                                                 >  2:958512/1‑101 (MQ=255)
                                                   tttCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAG.GCCGGTGTCGCCAAGCATGGCAACTGGCTCTggg                                                 >  1:1422040/1‑101 (MQ=255)
                                                    ttCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAAG.C.CGGTGTCGCCAAGCATGGCAACTGGCTCTGGGc                                                >  2:740661/1‑101 (MQ=255)
                                                     tCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAG.GCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGcc                                               >  1:3530140/1‑101 (MQ=255)
                                                        tGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAG.GCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTgg                                            >  2:192300/1‑101 (MQ=255)
                                                         gACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAG.GCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGGCTGGa                                           >  2:1867835/1‑101 (MQ=255)
                                                         gACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAG.GCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGa                                           >  1:1126035/1‑101 (MQ=255)
                                                          aCCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAG.GCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGaa                                          >  1:2102549/1‑101 (MQ=255)
                                                           ccAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAG.GCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAAc                                         <  1:2381351/101‑1 (MQ=255)
                                                            cAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAG.GCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAAcc                                        <  1:4206862/101‑1 (MQ=255)
                                                            cAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAG.GCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAAcc                                        <  2:3956242/101‑1 (MQ=255)
                                                             caCCCCAAGGGCCCCGCCGGGGGCGATCCTGTCGGCTTCGCGCTGGCGCCGAAC.AAG.GCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAAcct                                       <  1:259302/100‑2 (MQ=255)
                                                             aaCCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAG.GCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAAcct                                       <  1:4392759/101‑2 (MQ=255)
                                                             aaCCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAG.GCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAAcct                                       >  2:4177642/1‑100 (MQ=255)
                                                              aCCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAG.GCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAAcctc                                      <  2:2225738/101‑3 (MQ=255)
                                                              aCCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAG.GCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAAcctc                                      <  1:3084742/101‑3 (MQ=255)
                                                               ccccAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAG.GCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAAcctcg                                     <  1:2205054/101‑4 (MQ=255)
                                                                 ccAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAG.GCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCCGGAA.CCTCGcc                                  >  2:3068099/1‑101 (MQ=255)
                                                                  cAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAG.GCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCa                                 <  2:3850218/101‑1 (MQ=255)
                                                                    aGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAG.GCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCatt                               >  2:423589/1‑99 (MQ=255)
                                                                    aGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAG.GCCGGTGTCCCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCatt                               <  1:4302559/101‑3 (MQ=255)
                                                                     ggACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAG.GCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCattc                              <  1:1001458/101‑4 (MQ=255)
                                                                     ggACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCCGGCGCCGAAC.AAG.GCCGGGGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCattc                              >  1:463061/1‑98 (MQ=255)
                                                                       aCTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAG.GCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCcg                           <  1:680676/101‑1 (MQ=255)
                                                                        cTCGGCCGTGGCCGATCATTTCGGCTTCGCGCTGGCGCCGAAC.AAG.GCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCcgg                          >  1:1905480/1‑101 (MQ=255)
                                                                        cTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAG.GCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCcgg                          >  2:269498/1‑101 (MQ=255)
                                                                         tCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAG.GCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCcggc                         <  2:1615929/101‑1 (MQ=255)
                                                                          cGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAG.GCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCcggcg                        <  1:1563345/101‑1 (MQ=255)
                                                                           ggCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAG.GCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCcggcgg                       >  2:1465361/1‑101 (MQ=255)
                                                                            gCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAG.GCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCggg                      >  1:207315/1‑101 (MQ=255)
                                                                            gCCGTGGCCGATCATGTAGGCTTCGCGCTGGCGCCGAAC.AAG.GCCCGCGTCGCCAAGCATGGCAACTGGGTCTGGGCCTGG.ACCCTCGCC.ATTCCGGCggg                      >  2:3101666/1‑101 (MQ=255)
                                                                             ccGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAG.GCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGc                     <  2:4328380/101‑1 (MQ=255)
                                                                             ccGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAG.GCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGc                     <  1:2163702/101‑1 (MQ=255)
                                                                              cGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAG.GCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCa                    <  1:4439364/101‑1 (MQ=255)
                                                                              cGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAG.GCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCa                    >  2:649540/1‑101 (MQ=255)
                                                                              cGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAG.GCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCa                    >  1:1941318/1‑101 (MQ=255)
                                                                                tGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAG.GCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACg                  >  1:120355/1‑101 (MQ=255)
                                                                                  gCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAG.GCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCa                <  2:3237863/101‑1 (MQ=255)
                                                                                  gCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAG.GCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCa                >  1:768147/1‑101 (MQ=255)
                                                                                     gATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAG.GCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaa             >  2:1012597/1‑101 (MQ=255)
                                                                                      aTCATGTCGGCTTCGCGCTGGCGCCGAAC.AAG.GCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCATGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaag            >  1:4006217/1‑100 (MQ=255)
                                                                                       tCATGTCGGCTTCGCGCTGGCGCCGAAC.AAG.GCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaagg           >  2:328991/1‑99 (MQ=255)
                                                                                         aTGTCGGCTTCGCGCTGGCGCCGAAC.AAG.GCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaaggcc         <  2:3731701/101‑5 (MQ=255)
                                                                                          tGTCGGCTTCGCGCTGGCGCCGAAC.AAG.GCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCg       <  2:2102399/101‑1 (MQ=255)
                                                                                           gTCGGCTTCGCGCTGGCGCCGAAC.AAG.GCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGa      >  2:3818746/1‑101 (MQ=255)
                                                                                            tcggcttcgcgcTGGCGCCGAAC.AAG.GCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAg     <  1:1380101/101‑1 (MQ=255)
                                                                                            tcggcttcgcgcTGGCGCCGAAC.AAG.GCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAg     >  1:290445/1‑101 (MQ=255)
                                                                                            tcggcttcgcgcTGGCGCCGAAC.AAG.GCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAg     <  1:3193337/101‑1 (MQ=255)
                                                                                              ggcttcgcgcTGGCGCCGAAC.AAG.GCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCAGCGGGCACGC.AAAAGGCCGAggc   <  1:1707979/101‑1 (MQ=255)
                                                                                               gcttcgcgcTGGCGCCGATC.AAG.GCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAggcg  <  2:4385183/101‑1 (MQ=255)
                                                                                               gcttcgcgcTGGCGCCGAAC.AAG.GCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAggcg  >  1:1310697/1‑101 (MQ=255)
                                                                                                     |                                                                                                   
CTTCCAGCAGGGCCATTGCGGCATGTGGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCG.GCTGGC.CCGAAC.AAG.G.CGGTGTCCGCAAGCATGGCAACTGGCTCTGGGCCTGGAACCCTCGCCAATTCCGGCGGGCACGCAAAAAGGCCGAGGCG  >  NC_014034/2180061‑2180256

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG