Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,180,177 +C 100% coding (866/1314 nt) RCAP_rcc02022 → polyols ABC transporter periplasmic polyols‑binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,180,1771.→C100.0% 723.5 193coding (866/1314 nt)RCAP_rcc02022polyols ABC transporter periplasmic polyols‑binding protein
Reads supporting (aligned to +/- strand):  new base (89/102):  ref base (1/1):  total (90/103)

Only 100 of 214 total aligned reads displayed.

GGCATGTGGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTC.G.GCTGGC.CCGAAC.AAGG.CGGTGTCCGCAAGCATGGCAACTGGCTCTGGGCCTGGAACCCTCGCCAATTCCGGCGGGCACGCAAAAAGGCCGAGGCGGCGCTGAAATTCGTCGAAT  >  NC_014034/2180080‑2180275
                                                                                                      |                                                                                                  
ggCATGTGGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTC.GCGCTGGCGCCGAAC.AAGG.c                                                                                                   <  1:3810888/101‑1 (MQ=255)
  cATGTGGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTC.GCGCTGGCGCCGAAC.AAGG.ccg                                                                                                 >  2:3856819/1‑99 (MQ=255)
  cATGTGGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTC.GCGCTGGCGCCGAAC.AAGG.ccg                                                                                                 >  2:2163552/1‑99 (MQ=255)
    tgtgGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTC.GCGCTGGCGCCGAAC.AAGG.ccggt                                                                                               <  1:1907238/101‑5 (MQ=255)
    tgtgGAGCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTC.GCGCTGGCGCCGAAC.AAGG.ccggt                                                                                               <  1:4430785/101‑5 (MQ=255)
    tgtgGACCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTC.GCGCTGGCGCCGAAC.AAGG.ccggt                                                                                               <  1:2389308/101‑5 (MQ=255)
      tgGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGCGGCCGATCATGTCGGCTTC.GCGCTGGCGCCGAAC.AAGG.ccggggt                                                                                             >  1:3722433/1‑95 (MQ=255)
        gATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTC.GCGCTGGCGCCGAAC.AAGGCCGGTGTcg                                                                                            >  2:1563240/1‑100 (MQ=255)
         aTCGATGCGACCGTGGCCGCCTCTTTCGTGAGCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTC.GCGCTGGCGCCGAAC.AAGGCCGGTGTcgc                                                                                           <  2:2364485/101‑3 (MQ=255)
           cGATGCGACCGTGGCCGCCTCTTTCGTGCCCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTG.GCGCTGGCGCCGAAC.AAGGCCGGTGTcgcca                                                                                         >  1:4607684/1‑97 (MQ=255)
             aTGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTC.GCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAg                                                                                       <  2:2875913/101‑1 (MQ=255)
             aTGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTC.GCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAg                                                                                       >  2:2935105/1‑101 (MQ=255)
              tGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTC.GCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGc                                                                                      <  2:1951974/101‑1 (MQ=255)
               gCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTC.GCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCa                                                                                     >  2:1001375/1‑101 (MQ=255)
                cGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTC.GCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAATCAt                                                                                    <  2:146445/101‑1 (MQ=255)
                 gACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTC.GCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCCAGCATg                                                                                   >  1:2960223/1‑101 (MQ=255)
                 gACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTC.GCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATg                                                                                   <  1:1510445/101‑1 (MQ=255)
                 gACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTC.GCGCCGGCGCCGAAC.AAGGCCGGTGGCGCCAAGCATg                                                                                   >  1:556369/1‑101 (MQ=255)
                    cGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTC.GCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCa                                                                                >  1:1612567/1‑101 (MQ=255)
                       ggCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTC.GCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACt                                                                             <  2:3895840/101‑1 (MQ=255)
                        gccgccTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTC.GCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTg                                                                            <  1:2370329/101‑1 (MQ=255)
                         ccgccTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTC.GCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTgg                                                                           <  2:893810/101‑1 (MQ=255)
                           gccTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTC.GCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGct                                                                         >  1:862823/1‑101 (MQ=255)
                           gccTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTC.GCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGct                                                                         <  1:2546855/101‑1 (MQ=255)
                            ccTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTC.GCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGctc                                                                        >  1:4341519/1‑101 (MQ=255)
                              tctTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTC.GCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTg                                                                      <  1:139785/101‑1 (MQ=255)
                              tctTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTC.GCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACAGGCTCTg                                                                      >  2:4302270/1‑101 (MQ=255)
                               ctTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTC.GCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTgg                                                                     >  2:4088544/1‑101 (MQ=255)
                                tttCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTC.GCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTggg                                                                    >  2:29788/1‑101 (MQ=255)
                                tttCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTC.GCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTggg                                                                    >  2:958512/1‑101 (MQ=255)
                                tttCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTC.GCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTggg                                                                    >  1:1422040/1‑101 (MQ=255)
                                 ttCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTC.GCGCTGGCGCCGAACAAAGC.CGGTGTCGCCAAGCATGGCAACTGGCTCTGGGc                                                                   >  2:740661/1‑101 (MQ=255)
                                     tGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCTC.GCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCCACTGGCTCTGGGCCTgg                                                               >  1:4541031/1‑101 (MQ=255)
                                      gACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTC.GCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGGCTGGa                                                              >  2:1867835/1‑101 (MQ=255)
                                      gACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTC.GCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGa                                                              >  1:1126035/1‑101 (MQ=255)
                                      gACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTC.GCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGa                                                              >  1:3786385/1‑101 (MQ=255)
                                       aCCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTC.GCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGaa                                                             >  1:2102549/1‑101 (MQ=255)
                                       aCCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTC.GCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGaa                                                             <  1:4520109/101‑1 (MQ=255)
                                        ccAAGCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTC.GCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAAGTGGCTCTGGGCCGGGAAc                                                            <  2:229305/101‑1 (MQ=255)
                                         cAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTC.GCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAAcc                                                           <  2:3956242/101‑1 (MQ=255)
                                         cAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTC.GCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAAcc                                                           <  1:4206862/101‑1 (MQ=255)
                                          caCCCCAAGGGCCCCGCCGGGGGCGATCCTGTCGGCTTC.GCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAAcct                                                          <  1:259302/100‑2 (MQ=255)
                                           aCCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTC.GCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAAcctc                                                         <  2:4230445/101‑3 (MQ=255)
                                           aCCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTC.GCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAAcctc                                                         <  1:3084742/101‑3 (MQ=255)
                                            ccccAAGGACTCGGCCGTGGCCGATCATGTCGGCTTC.GCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAAcctcg                                                        <  1:2205054/101‑4 (MQ=255)
                                              ccAAGGACTCGGCCGTGGCCGATCATGTCGGCTTC.GCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCCGGAA.CCTCGcc                                                     >  2:3068099/1‑101 (MQ=255)
                                                aaGGACTCGGCCGTGGCCGATCATGTCGGCTTC.GCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAAATGGCTCTGGGCCTGGAA.CCTCGCCat                                                   >  1:3914950/1‑100 (MQ=255)
                                                aaGGACTCGGCCGCGGCCGATCATGTCGGCTTC.GCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCat                                                   <  2:3722158/101‑2 (MQ=255)
                                                  ggACTCGGCCGTGGCCGATCATGTCGGCTTC.GCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCattc                                                 <  1:1001458/101‑4 (MQ=255)
                                                    aCTCGGCCGTGGCCGATCATGTCGGCTTC.GCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCcg                                              <  1:680676/101‑1 (MQ=255)
                                                     cTCGGCCGTGGCCGATCATGTCGGCTTC.GCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCcgg                                             >  1:2688981/1‑101 (MQ=255)
                                                      tCGGCCGTGGCCGATCATGTCGGCTTC.GCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCcggc                                            <  2:1615929/101‑1 (MQ=255)
                                                      tCGGCCGTGGCCGATCATGTCGGCTTC.GCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCcggc                                            >  2:1363171/1‑101 (MQ=255)
                                                        ggCCGTGGCCGATCATGTCGGCTTC.GCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCcggcgg                                          >  2:1295013/1‑101 (MQ=255)
                                                         gCCGTGGCCGATCATGTAGGCTTC.GCGCTGGCGCCGAAC.AAGGCCCGCGTCGCCAAGCATGGCAACTGGGTCTGGGCCTGG.ACCCTCGCC.ATTCCGGCggg                                         >  2:3101666/1‑101 (MQ=255)
                                                          ccGTGGCCGATCATGTCGGCTTC.GCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGc                                        <  1:2163702/101‑1 (MQ=255)
                                                           cGTGGCCGATCATGTCGGCTTC.GCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCa                                       >  2:649540/1‑101 (MQ=255)
                                                           cGTGGCCGATCATGTCGGCTTC.GCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCa                                       <  1:4439364/101‑1 (MQ=255)
                                                             tGGCCGATCATGTCGGCTTC.GCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACg                                     <  2:3547784/101‑1 (MQ=255)
                                                             tGGCCGATCATGTCGGCTTC.GCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACg                                     >  1:120355/1‑101 (MQ=255)
                                                              ggCCGATCATGTCGGCTTC.GCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGc                                    >  1:768514/1‑101 (MQ=255)
                                                               gCCGATCATGTCGGCTTC.GCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCa                                   <  2:300214/101‑1 (MQ=255)
                                                               gCCGATCATGTCGGCTTC.GCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCa                                   <  2:3237863/101‑1 (MQ=255)
                                                               gCCGATCATGTCGGCTTC.GCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCa                                   <  1:1945081/101‑1 (MQ=255)
                                                                ccGATCATGTCGGCTTC.GCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaa                                  >  1:3545219/1‑101 (MQ=255)
                                                                   aTCATGTCGGCTTC.GCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCATGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaag                               >  1:4006217/1‑100 (MQ=255)
                                                                      aTGTCGGCTTC.GCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaaggcc                            <  2:3731701/101‑5 (MQ=255)
                                                                        gTCGGCTTC.GCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGa                         >  2:3818746/1‑101 (MQ=255)
                                                                         tcggcttc.gcgcTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.CAAAGGCCGAg                        >  1:3621353/1‑101 (MQ=255)
                                                                         tcggcttc.gcgcTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAg                        <  2:4341227/101‑1 (MQ=255)
                                                                         tcggcttc.gcgcTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAg                        <  1:3193337/101‑1 (MQ=255)
                                                                         tcggcttc.gcgcTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAg                        >  1:290445/1‑101 (MQ=255)
                                                                         tcggcttc.gcgcTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAg                        <  1:1380101/101‑1 (MQ=255)
                                                                           ggcttc.gcgcTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCAGCGGGCACGC.AAAAGGCCGAggc                      <  1:1707979/101‑1 (MQ=255)
                                                                            gcttc.gcgcTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAggcg                     >  1:1310697/1‑101 (MQ=255)
                                                                              cttcgcgctGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAggcgg                    >  1:2467832/7‑101 (MQ=255)
                                                                              cttcgcgctGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAggcgg                    <  2:4484894/95‑1 (MQ=255)
                                                                              cttcgcgctGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAggcgg                    <  1:483125/95‑1 (MQ=255)
                                                                                tcgcgctGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGgcggcg                  <  1:1561683/97‑1 (MQ=255)
                                                                                  gcgctGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCt                <  2:831617/99‑1 (MQ=255)
                                                                                  gcgctGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCt                <  2:4368466/99‑1 (MQ=255)
                                                                                   cgctGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCNGCGGGCACGC.AAAAGGCCGAGGCGGCGCTg               >  1:965467/2‑101 (MQ=255)
                                                                                   cgctGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTg               <  2:963268/100‑1 (MQ=255)
                                                                                   cgctGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTg               >  2:3343742/2‑101 (MQ=255)
                                                                                   cgctGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTg               >  2:2210618/2‑101 (MQ=255)
                                                                                   cgctGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTg               <  2:1013234/100‑1 (MQ=255)
                                                                                    gctGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGa              <  2:2477618/101‑1 (MQ=255)
                                                                                    gctGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGa              >  1:3314842/1‑101 (MQ=255)
                                                                                    gctGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGa              >  1:1280689/1‑101 (MQ=255)
                                                                                      tggcgccGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGGCGAGGCGGCGCTGaaa            >  2:2612107/6‑101 (MQ=255)
                                                                                      tggcgccGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGaaa            <  1:1012680/96‑1 (MQ=255)
                                                                                       ggcgccGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAAt           >  1:3821779/5‑101 (MQ=255)
                                                                                         cgccGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATtc         <  1:3869176/99‑1 (MQ=255)
                                                                                          gccGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATtcg        >  1:3884854/2‑101 (MQ=255)
                                                                                          gccGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATtcg        <  2:207291/100‑1 (MQ=255)
                                                                                           ccGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATtcgt       >  2:519131/1‑101 (MQ=255)
                                                                                             gAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATtcgtcg     <  1:728276/101‑1 (MQ=255)
                                                                                             gAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATtcgtcg     <  2:1125946/101‑1 (MQ=255)
                                                                                               aC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGaa   >  1:3493632/1‑101 (MQ=255)
                                                                                                c.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAAt  <  1:3150170/101‑1 (MQ=255)
                                                                                                      |                                                                                                  
GGCATGTGGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTC.G.GCTGGC.CCGAAC.AAGG.CGGTGTCCGCAAGCATGGCAACTGGCTCTGGGCCTGGAACCCTCGCCAATTCCGGCGGGCACGCAAAAAGGCCGAGGCGGCGCTGAAATTCGTCGAAT  >  NC_014034/2180080‑2180275

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG