Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,249,327 +C 5.3% intergenic (‑123/‑182) parE ← / → RCAP_rcc02080 DNA topoisomerase IV subunit B/HAD superfamily hydrolase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,249,3273.→C 5.3% 28.0 ‑1.5 ‑0.0207intergenic (‑123/‑182)parE/RCAP_rcc02080DNA topoisomerase IV subunit B/HAD superfamily hydrolase
Reads supporting (aligned to +/- strand):  new base (3/8):  ref base (93/103):  total (96/111)
Fisher's exact test strand distribution p-value = 2.28e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.34e-01

Only 100 of 223 total aligned reads displayed.

TGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.CCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.CCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAAC  >  NC_014034/2249229‑2249426
                                                                                                     |                                                                                                           
tGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...g                                                                                                          <  2:2483596/101‑1 (MQ=255)
tGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...g                                                                                                          >  2:1400778/1‑101 (MQ=255)
tGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...g                                                                                                          >  2:1811288/1‑101 (MQ=255)
  agaTGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...ggg                                                                                                        >  2:4386802/1‑101 (MQ=255)
      gCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCCGGATCTTTGAGGCAAGATGAA...AGCG..GGc                                                                                                  >  2:2031148/1‑101 (MQ=37)
        acaGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCtt                                                                                                <  1:1240985/101‑1 (MQ=255)
        acaGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCtt                                                                                                >  2:1049655/1‑101 (MQ=255)
          aGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTc                                                                                              <  1:318676/101‑1 (MQ=255)
           gATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCa                                                                                             <  1:3143737/101‑1 (MQ=255)
           gATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCa                                                                                             <  1:1136761/101‑1 (MQ=255)
           gATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGACCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCa                                                                                             >  2:2086170/1‑101 (MQ=255)
             tcccGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGTAAGATGAA...GGGG..GGCTTTCATc                                                                                           <  2:4171107/99‑1 (MQ=255)
               ccGCAGCTTGGGCCCCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCtt                                                                                         <  1:2366249/101‑1 (MQ=255)
               ccGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCtt                                                                                         <  1:2479760/101‑1 (MQ=255)
               ccGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCtt                                                                                         <  2:3582004/101‑1 (MQ=255)
                 gCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGc                                                                                       >  1:4469196/1‑101 (MQ=255)
                 gCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGc                                                                                       <  2:3065563/101‑1 (MQ=255)
                  cAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGcc                                                                                      <  2:2998724/101‑1 (MQ=255)
                   aGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCg                                                                                     >  1:819492/1‑101 (MQ=255)
                   aGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGAAGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCg                                                                                     >  2:4032114/1‑101 (MQ=38)
                    gCTTGGGCACGCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGa                                                                                    <  2:892112/101‑1 (MQ=255)
                    gCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGa                                                                                    <  2:4411749/101‑1 (MQ=255)
                     cTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGaa                                                                                   <  1:2939022/101‑1 (MQ=255)
                     cTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGaa                                                                                   >  2:523173/1‑101 (MQ=255)
                       tGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGaaaa                                                                                 >  1:4235150/1‑101 (MQ=255)
                        gggCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAat                                                                                >  2:1629410/1‑101 (MQ=255)
                        gggCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAat                                                                                >  2:3475674/1‑101 (MQ=255)
                        gggCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTAAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAat                                                                                >  1:3247440/1‑101 (MQ=255)
                          gCACCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAatat                                                                              <  1:863587/101‑1 (MQ=255)
                            aCCCGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATcc                                                                            <  1:3036161/101‑1 (MQ=255)
                             cccGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATccc                                                                           >  1:156643/1‑101 (MQ=255)
                              ccGGGGCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCg                                                                          >  1:3734220/1‑101 (MQ=255)
                                ggggCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCggg                                                                        >  2:2434816/1‑101 (MQ=255)
                                ggggCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCggg                                                                        >  2:6728/1‑101 (MQ=255)
                                ggggCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCggg                                                                        >  1:4362966/1‑101 (MQ=255)
                                  ggCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCggggg                                                                      <  2:780584/101‑1 (MQ=255)
                                  ggCAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCggggg                                                                      >  2:4265205/1‑101 (MQ=255)
                                    cAAGAAGGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGt                                                                    >  1:989718/1‑101 (MQ=255)
                                     aagaagGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCgggggggg                                                                   >  1:1649433/1‑99 (MQ=255)
                                     aagaagGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTg                                                                   <  1:1265535/101‑1 (MQ=255)
                                     aagaagGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTg                                                                   <  1:540217/101‑1 (MQ=255)
                                      agaagGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGTTATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGGGa                                                                  >  1:973071/1‑101 (MQ=38)
                                      agaagGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGa                                                                  <  2:3774895/101‑1 (MQ=255)
                                      agaagGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGa                                                                  <  1:1400873/101‑1 (MQ=255)
                                        aagGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.t                                                               <  1:191509/101‑1 (MQ=255)
                                         agGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTTAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.Tg                                                              <  2:2233459/101‑1 (MQ=255)
                                         agGGGGCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.Tg                                                              <  2:839540/101‑1 (MQ=255)
                                          gggggCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.Tgg                                                             <  1:2957826/101‑1 (MQ=255)
                                             ggCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGgcgc                                                          >  1:2748308/1‑101 (MQ=255)
                                             ggCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGgcgc                                                          <  1:2191454/101‑1 (MQ=255)
                                             ggCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGGGAA.TGgcgc                                                          >  1:2461494/1‑101 (MQ=255)
                                              gCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCa                                                         >  1:1977196/1‑101 (MQ=255)
                                              gCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCa                                                         <  2:2587633/101‑1 (MQ=255)
                                              gCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCa                                                         <  1:4451019/101‑1 (MQ=255)
                                              gCTCTGCCCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCa                                                         <  2:262919/101‑1 (MQ=255)
                                               ctctGCCCCCGTCCCGTNCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..g                                                      <  2:25262/101‑1 (MQ=255)
                                                 ctgcccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..Gcc                                                    <  2:3403963/97‑1 (MQ=255)
                                                   gcccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCag                                                  >  2:3371068/3‑101 (MQ=255)
                                                    cccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCaga                                                 >  1:649210/2‑101 (MQ=255)
                                                     ccccGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCagag                                                >  1:3318288/1‑101 (MQ=255)
                                                      cccgtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAgg                                               <  2:2348383/101‑1 (MQ=255)
                                                       ccgtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAggg                                              >  2:3700126/1‑101 (MQ=255)
                                                        cgtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAgggg                                             >  1:493782/1‑101 (MQ=255)
                                                         gtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCGGAggggg                                            <  2:1448065/101‑1 (MQ=255)
                                                          tcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAgggggg                                           >  1:3661675/1‑101 (MQ=255)
                                                           cccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGc                                          >  2:4579212/1‑101 (MQ=255)
                                                           cccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGc                                          <  1:1201492/101‑1 (MQ=255)
                                                           cccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGGGAA.TGGCGCA..GCCAGAggggggg                                          >  1:351778/1‑100 (MQ=255)
                                                            ccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGcg                                         >  2:943842/1‑100 (MQ=255)
                                                            ccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCa                                         <  2:156629/101‑1 (MQ=255)
                                                            ccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCa                                         >  1:4291518/1‑101 (MQ=255)
                                                            ccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCa                                         <  2:3234555/101‑1 (MQ=255)
                                                             cgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAg                                        <  1:2241603/101‑1 (MQ=255)
                                                             cgtCCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...TGGG..CGCTTTCATCTTCCCGAAAATATCCCGGGGGGTGAA.TGGCCCA..GCCAGAGGGGGGCAg                                        <  2:3852007/101‑1 (MQ=25)
                                                             cgcaagcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAggggg                                            <  2:2140257/94‑1 (MQ=18)
                                                              gtGCCGGGACT.CCCCTGCGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAgc                                       <  1:3869997/101‑1 (MQ=255)
                                                              gcaagcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAgggggg                                           >  2:2807528/7‑101 (MQ=18)
                                                              gcaagcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAgggggg                                           >  2:401988/7‑101 (MQ=18)
                                                                gCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAgcg.c                                    >  2:594752/1‑101 (MQ=255)
                                                                gCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAgcg.c                                    >  1:3413164/1‑101 (MQ=255)
                                                                  gcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAGGGGGGCAgc                                       <  2:1690262/99‑1 (MQ=18)
                                                                  ccgggctcccccaGGGATATTTGAGGCAAGATGAG...GGGG..GGCTTTCATCTTGACGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.cc                                   <  1:739917/93‑1 (MQ=25)
                                                                  cGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.ccc                                  >  2:3060633/1‑101 (MQ=255)
                                                                  cGGGACG.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.ccc                                  <  1:3475932/101‑1 (MQ=255)
                                                                   gggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.cccc                                 >  1:3856632/1‑101 (MQ=255)
                                                                   gggACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.cccc                                 >  1:4100865/1‑101 (MQ=255)
                                                                   cggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..GCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAGGGGGGCAgcg                                      <  1:308289/100‑1 (MQ=18)
                                                                        t.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.ccccccttc                            <  1:1780179/101‑5 (MQ=255)
                                                                          cccccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.ccccccttcc                           >  2:1358030/1‑96 (MQ=255)
                                                                            cccGGGATATTTGCGGCAAGATGAA...GGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAAAGGCCAAGAGGGGGCAGCG.CCCCCtgc                             >  2:1905000/1‑99 (MQ=0)
                                                                            cccGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTcgcg                          >  2:4227192/1‑101 (MQ=255)
                                                                              cGGGATATTTGCGGCAAGATGAA...GGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAAAGGCCAAGAGGGGGCAGCG.CCCCCtgccc                           >  2:3971647/1‑97 (MQ=0)
                                                                              cGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCgcggg                        >  1:1804492/1‑101 (MQ=255)
                                                                               gggATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCgcgggc                       >  1:4656833/1‑101 (MQ=255)
                                                                                ggATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCgcgggcg                      >  1:2624461/1‑101 (MQ=255)
                                                                                              aGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCGCGGGCGGCCGCGACgcgcgc        <  1:6732/101‑1 (MQ=255)
                                                                                               gATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCGCGGGCGGCCGCGACGCGcgcc       >  2:1985429/1‑101 (MQ=255)
                                                                                               gATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCGCCCCCCTTCGCGGGCGGCCGCGACGCGcgcc       <  1:2975977/101‑1 (MQ=255)
                                                                                                   aa...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGAGCAGCGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCaa   >  1:3556136/1‑101 (MQ=255)
                                                                                                    a...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGAAGCGCCCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAAc  <  1:4115703/101‑1 (MQ=255)
                                                                                                     |                                                                                                           
TGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.CCCCGTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..GGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCG.CCCCCTTCGCGGGCGGCCGCGACGCGCGCCGCAAC  >  NC_014034/2249229‑2249426

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG