Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,304,896 +C 100% coding (2679/2808 nt) RCAP_rcc02139 ← methyl‑accepting chemotaxis protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,304,8961.→C100.0% 556.0 159coding (2679/2808 nt)RCAP_rcc02139methyl‑accepting chemotaxis protein
Reads supporting (aligned to +/- strand):  new base (67/91):  ref base (0/1):  total (67/92)

Only 100 of 190 total aligned reads displayed.

GGCAAGATCCTGATAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCGCCCTGCATGC.G.AAGACCG.CGTAC.GCTCCCGCA.GGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTG  >  NC_014034/2304797‑2304988
                                                                                                      |                                                                                              
 gCAAGGTCCTGATAATGTTGCGAAAACCGGGGCCGGACCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGG.AAGACcg.cc                                                                                              <  2:907513/101‑2 (MQ=255)
    aGATCCTGATAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGG.GGCCTGCATGCGG.AAGACcg.ccgtc                                                                                           >  2:3717897/1‑97 (MQ=255)
     gATCCTGATAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGG.AAGACcg.ccgtca                                                                                          <  1:2158714/101‑6 (MQ=255)
     aaTCCTGATAATCTTGCGAAAACCCCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGG.AAGACcg.ccgtca                                                                                          <  1:1325639/100‑6 (MQ=255)
      aTCCTGATAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGG.AAGACcg.ccgtcag                                                                                         >  2:3810751/1‑95 (MQ=255)
        ccTGATAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGG.AAGACcg.ccggcagcc                                                                                       >  2:3328061/1‑93 (MQ=255)
             tAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGG.AAGAACGCCGTCA.GCTCCCGc                                                                                  >  2:233760/1‑101 (MQ=255)
             tAATCTTGCGCAAAACCCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGG.AAGACCGCCGTCA.GCTCCCGc                                                                                  <  1:1375771/101‑1 (MQ=255)
             tAATCTTGCGAAAAACTCGCCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGG.AAGACCGCCGTCA.GCTCCCGc                                                                                  <  2:2935297/101‑1 (MQ=255)
              aaTCTTGCGCAAACCTCGGCCGGATCCGGCCGGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGG.GGCCTGCATGCGG.AAGACCGCCGTCA.GCTCCCGCa                                                                                 <  1:3718172/101‑1 (MQ=255)
              aaTCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGG.AAGACCGCCGTCA.GCTCCCGCa                                                                                 >  2:4608335/1‑101 (MQ=255)
               aTCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGG.AAGACCGCCGTCA.GCTCCCGCA.g                                                                               >  1:2373229/1‑101 (MQ=255)
                tCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGG.AAGACCGCCGTCA.GCTCCCGCA.gg                                                                              >  2:36489/1‑101 (MQ=255)
                tCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGCGGCG.CCTGCATGCGG.AAGACCGCCGTCA.GCTCCCGCA.gg                                                                              >  1:2495508/1‑101 (MQ=255)
                 cTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGG.AAGACCGCCGTCA.GCTCCCGCA.ggg                                                                             >  2:3871187/1‑100 (MQ=255)
                  ttGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGG.AAGACCGCCGTCA.GCTCCCGCA.gggt                                                                            >  1:3349105/1‑99 (MQ=255)
                  ttGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTCGC.CCCTGCATGCGG.AAGACCGCCGTCA.GCTCCCGCA.gggc                                                                            >  2:321730/1‑99 (MQ=255)
                   tGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGG.AAGACCT.CCCCCAGCTCCCGCA.gggac                                                                           >  2:3906710/1‑98 (MQ=255)
                   tGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGG.AAGACCGCCGTCA.GCCCCCGCA.gggtc                                                                           >  1:3845079/1‑98 (MQ=255)
                      gcAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGG.AAGACCGCCGTCA.GCTCCCGCAGGGTCTgc                                                                         >  2:1012848/1‑101 (MQ=255)
                      caaaacCTCGGCCGGACCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGG.AAGACCGCCGTCA.GCTCCCGCAGGGTCTgc                                                                         <  2:3965909/99‑1 (MQ=255)
                       cAAACCTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGG.AAGACCGCCGTCA.GCTCCCGCAGGGTCTgcg                                                                        <  2:3693257/101‑1 (MQ=255)
                        aaaaCTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGG.AAGACCGCCGTCA.GCTCCCGCA.ggggctgcgc                                                                      >  2:3625750/1‑93 (MQ=255)
                          aaCTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGG.AAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCt                                                                     >  1:3140013/1‑101 (MQ=255)
                          aaCTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGG.AAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCt                                                                     >  1:702146/1‑101 (MQ=255)
                          aaCTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGG.AAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCt                                                                     >  2:3760999/1‑101 (MQ=255)
                            cTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGG.AAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCg                                                                   >  2:1218992/1‑101 (MQ=255)
                             ccgcccgaacccGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGG.AAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGc                                                                  <  1:3211123/91‑1 (MQ=255)
                              cGGCCGGCCCGGGCCTGTCAGCCGCCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGG.AAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGcc                                                                 <  2:4340240/101‑1 (MQ=255)
                              cGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGG.AAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGcc                                                                 >  2:1091928/1‑101 (MQ=255)
                              cGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGG.AAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGcc                                                                 >  1:1032287/1‑101 (MQ=255)
                              cGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGGGGCG.CCTGCATGCGG.AAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGcc                                                                 >  1:2964306/1‑101 (MQ=255)
                                gCCGGATCCGGCGTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGG.AAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCca                                                               <  1:2191981/101‑1 (MQ=255)
                                  cGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGG.AAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCcagc                                                             >  2:2405485/1‑101 (MQ=255)
                                  cGGATCCGGCCTGTCACCCGGCCTGCCGCAACGGCCGCGCCCGGGGCG.CCTGCATGCGG.GAGACCGCCGTCA.GCGCCCGCAGGGTCTGCGCCTCGCCcaga                                                             >  2:150026/1‑100 (MQ=255)
                                   ggATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGG.AAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCcagca                                                            >  1:1609700/1‑101 (MQ=255)
                                    gATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGG.AAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCcagcag                                                           >  2:264718/1‑101 (MQ=255)
                                        cgcccTGTCACCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGG.AAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGcc                                                       <  1:1911371/98‑1 (MQ=255)
                                        cGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCTGTGGCG.CCTGCATGC.GCAAGACCGCCGTCA.GCTCCCGCAGGGGCTGCGCCCCGCCCAGCAGCGcc                                                       >  2:3100648/1‑101 (MQ=255)
                                         ggCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGG.AAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCt                                                      >  1:1004543/1‑101 (MQ=255)
                                          gCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGG.AAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTg                                                     >  2:3068248/1‑101 (MQ=255)
                                          gCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGG.AAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTg                                                     <  1:761117/101‑1 (MQ=255)
                                          gCCGGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGG.AAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTg                                                     <  1:3624503/101‑1 (MQ=255)
                                           ccggTCAGCGGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGG.AAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGc                                                    <  2:3014776/98‑1 (MQ=255)
                                            cTGTCAGCCGCCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGG.AAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCg                                                   <  1:1969233/101‑1 (MQ=255)
                                              gTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGG.AAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTc                                                 <  2:4520872/101‑1 (MQ=255)
                                               tCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGG.AAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTcg                                                <  1:1673373/101‑1 (MQ=255)
                                                cAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGG.AAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTcgc                                               <  1:4250937/101‑1 (MQ=255)
                                                 aGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGG.AAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTcgcg                                              <  1:433086/101‑1 (MQ=255)
                                                     ggCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGG.AAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCcg                                          >  2:4390974/1‑101 (MQ=255)
                                                     ggCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGG.AAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCcg                                          <  1:2942734/101‑1 (MQ=255)
                                                     ggCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGG.AAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCcg                                          <  1:300324/101‑1 (MQ=255)
                                                      gCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGG.AAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCcgc                                         <  2:1595678/101‑1 (MQ=255)
                                                        cTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGG.AAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGt                                       >  2:3483902/1‑101 (MQ=255)
                                                        cTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGG.AAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGt                                       >  2:1758870/1‑101 (MQ=255)
                                                         tGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGG.AAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGtt                                      >  2:1717084/1‑101 (MQ=255)
                                                          gCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGG.AAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGttt                                     <  1:3209208/101‑1 (MQ=255)
                                                            cGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGG.AAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTc                                   >  1:1494635/1‑101 (MQ=255)
                                                              cAACGGCGGCGCCGGTGGCG.CCTGCATGCGG.AAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCt                                 >  2:2218656/1‑101 (MQ=255)
                                                              cAACGGCGGCGCCGGTGGCG.CCTGCATGCGG.AAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCt                                 <  2:1981753/101‑1 (MQ=255)
                                                               aaCGGCGGCGCCGGTGGCG.CCTGCATGCGG.AAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTc                                <  1:978221/101‑1 (MQ=255)
                                                               aaCGGCGGCGCCGGTGGCG.CCTGCATGCGG.AAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTc                                <  1:264750/101‑1 (MQ=255)
                                                               aaCGGCGGCGCCGGTGGCG.CCTGCATGCGG.AAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTc                                <  1:1012931/101‑1 (MQ=255)
                                                               aaCGGCGGCGCCGGTGGCG.CCTGCATGCGG.AAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTc                                >  1:4655028/1‑101 (MQ=255)
                                                                aCGGCGGCGCCGGTGGCG.CCTGCATGCGG.AAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCg                               <  1:1219084/101‑1 (MQ=255)
                                                                 cggcggCGCCGGTGGCG.CCTGCATGCGG.AAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGa                              <  1:3904947/101‑1 (MQ=255)
                                                                  ggcggcGCCGGTGGCG.CCTGCATGCGG.AAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGaa                             <  1:2597186/101‑1 (MQ=255)
                                                                   gcggcgCCGGTGGCG.CCTGCATGCGG.AAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAAc                            <  2:1609595/101‑1 (MQ=255)
                                                                     ggcgCCGGTGGCG.CCTGCATGCGG.AAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACAt                          <  2:4654727/101‑1 (MQ=255)
                                                                     ggcgCCGGTGGCG.CCAGCATGCGG.AAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACAt                          >  1:3934834/1‑101 (MQ=255)
                                                                      gcgcCGGTGGCG.CCTGCATGCGG.AAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATc                         <  1:1311792/101‑1 (MQ=25)
                                                                       cgcCGGTGGCG.CCTGCATGCGG.AAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATcg                        <  1:3306308/101‑1 (MQ=25)
                                                                        gcCGGTGGCG.CCTGCATGCGG.AAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATcgc                       <  1:993372/101‑1 (MQ=25)
                                                                        gcCGGTGGCG.CCTGCATGCGG.AAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATcgc                       <  2:1293582/101‑1 (MQ=25)
                                                                        gcCGGTGGCG.CCTGCATGCGG.AAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATcgc                       >  1:2016415/1‑101 (MQ=25)
                                                                          cGGTGGCG.CCTGCATGCGG.AAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCACCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATcgccg                     <  1:3100892/101‑1 (MQ=25)
                                                                           ggTGGCG.CCTGCATGCGG.AAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATcgccgc                    <  1:2393775/101‑1 (MQ=25)
                                                                           ggTGGCG.CCTGCATGCGG.AAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATcgccgc                    >  2:761390/1‑101 (MQ=25)
                                                                           ggTGGCG.CCTGCATGCGG.AAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATcgccgc                    >  1:4302170/1‑101 (MQ=25)
                                                                            gTGGCG.CCTGCATGCGG.AAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCa                   >  2:3445105/1‑101 (MQ=25)
                                                                            gTGGCG.CCTGCATGCGG.AAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCa                   >  1:4567167/1‑101 (MQ=25)
                                                                             tGGCG.CCTGCATGCGG.AAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCAt                  >  1:2107733/1‑101 (MQ=21)
                                                                               ggcgccTGCATGCGG.AAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCAtt                 >  1:4496137/5‑101 (MQ=25)
                                                                               ggcgccTGCATGCGG.AAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCAtt                 <  1:1419742/97‑1 (MQ=25)
                                                                                gcgccTGCATGCGG.AAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATtg                <  1:1719603/98‑1 (MQ=25)
                                                                                gcgccTGCATGCGG.AAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATtg                <  2:4574798/98‑1 (MQ=25)
                                                                                  gccTGCATGCGG.AAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATtgtg              >  2:451473/2‑101 (MQ=25)
                                                                                  cccTGCATGCGG.AAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATtgtg              <  2:1323112/101‑1 (MQ=25)
                                                                                   ccTGCATGCGG.AAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGc             >  2:4094683/1‑101 (MQ=21)
                                                                                    cTGCATGCGG.AAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGCCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCt            >  2:4332181/1‑101 (MQ=25)
                                                                                     tGCATGCGG.AAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTg           <  2:1129263/101‑1 (MQ=25)
                                                                                      gCATGCGG.AAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTgg          <  2:1406515/101‑1 (MQ=25)
                                                                                      gCATGCGG.AAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTgg          <  2:196101/101‑1 (MQ=25)
                                                                                       cATGCGG.AAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTggg         <  1:2122383/101‑1 (MQ=25)
                                                                                         tgcgg.AAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTg       <  2:4069613/97‑1 (MQ=25)
                                                                                         tgcgg.AAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTg       <  2:1038345/97‑1 (MQ=25)
                                                                                          gcgg.AAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGa      <  1:1748034/98‑1 (MQ=25)
                                                                                           cgg.AAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGAc     <  1:595327/99‑1 (MQ=21)
                                                                                           cgg.AAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGAc     <  2:2756275/99‑1 (MQ=21)
                                                                                           cgg.AAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGAc     <  1:3470920/99‑1 (MQ=25)
                                                                                                      |                                                                                              
GGCAAGATCCTGATAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCGCCCTGCATGC.G.AAGACCG.CGTAC.GCTCCCGCA.GGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTG  >  NC_014034/2304797‑2304988

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG