Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,304,910 +G 100% coding (2665/2808 nt) RCAP_rcc02139 ← methyl‑accepting chemotaxis protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,304,9101.→G100.0% 615.1 177coding (2665/2808 nt)RCAP_rcc02139methyl‑accepting chemotaxis protein
Reads supporting (aligned to +/- strand):  new base (75/102):  ref base (0/0):  total (75/102)

Only 100 of 201 total aligned reads displayed.

AATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCGCCCTGCATGC.GAAGACCG.CGTAC.GCTCCCGCA.GGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCAAGCTGGTTCACCG  >  NC_014034/2304811‑2305005
                                                                                                       |                                                                                               
aaTCTTGCGCAAACCCCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCA.GCTCCCGCa                                                                                                  <  1:1362138/101‑1 (MQ=255)
aaTCTTGCCAAAACCCCGGCCGGACCCGGCCGGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCA.GCTCCCGCa                                                                                                  <  2:3354762/101‑1 (MQ=255)
aaTCTTGCCAAAAACCCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCA.GCTCCCGCa                                                                                                  <  1:1917972/101‑1 (MQ=255)
  tCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGCGGCG.CCTGCATGCGGAAGACCGCCGTCA.GCTCCCGCA.gg                                                                                               >  1:2495508/1‑101 (MQ=255)
   cTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCA.GCTCCCGCA.ggg                                                                                              >  2:3871187/1‑100 (MQ=255)
    ttGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCA.GCTCCCGCA.gggt                                                                                             >  1:3349105/1‑99 (MQ=255)
    ttGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTCGC.CCCTGCATGCGGAAGACCGCCGTCA.GCTCCCGCA.gggc                                                                                             >  2:321730/1‑99 (MQ=255)
     tGCGCAACTCTGGGCGGGACCGGGCCTGTCAGCCGCCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCA.GCTCCCGCA.gggtc                                                                                            <  2:1156362/101‑4 (MQ=255)
     tGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCT.CCCCCAGCTCCCGCA.gggac                                                                                            >  2:3906710/1‑98 (MQ=255)
       cgcAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCA.GCTCCCGCA.gggtctg                                                                                          >  2:2222413/1‑96 (MQ=255)
        gcAAACCTCGGCGGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCA.GCTCCCGCAGGGTCTgc                                                                                          <  2:2117961/101‑1 (MQ=255)
        gcAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCA.GCTCCCGCAGGGTCTgc                                                                                          >  2:1012848/1‑101 (MQ=255)
        gcAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCA.GCTCCCGCAGGGTCTgc                                                                                          >  2:300292/1‑101 (MQ=255)
        caaaacCTCGGCCGGACCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCA.GCTCCCGCAGGGTCTgc                                                                                          <  2:3965909/99‑1 (MQ=255)
         cAAACCTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCA.GCTCCCGCAGGGTCTgcg                                                                                         <  2:3693257/101‑1 (MQ=255)
         cAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTTCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCA.GCTTCCGCAGGGTCTgcg                                                                                         >  2:3090304/1‑101 (MQ=255)
          aaaccccGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCA.GCTCCCGCAGGGTCTgcgc                                                                                        <  1:123745/95‑1 (MQ=255)
          aaaaCTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGGAGACCGCCGTCA.GCTCCCGCAGGGTCTgcgc                                                                                        >  2:3208965/1‑101 (MQ=255)
            aaCTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCt                                                                                      >  2:3760999/1‑101 (MQ=255)
            aaCTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCt                                                                                      >  2:4607209/1‑101 (MQ=255)
            aaCTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCt                                                                                      >  1:3140013/1‑101 (MQ=255)
               ccgcccgaacccGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGc                                                                                   <  1:3211123/91‑1 (MQ=255)
                cGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGcc                                                                                  >  2:1091928/1‑101 (MQ=255)
                cGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGGGGCG.CCTGCATGCGGAAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGcc                                                                                  >  1:2964306/1‑101 (MQ=255)
                 ggCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGccc                                                                                 >  2:1734153/1‑101 (MQ=255)
                     ggATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCcagca                                                                             >  2:1304725/1‑101 (MQ=255)
                      gATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCcagcag                                                                            >  2:264718/1‑101 (MQ=255)
                       aTCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCagcagc                                                                           >  2:2741159/1‑101 (MQ=255)
                        tCCGGCCTGTCAGCCGGCGTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAgcg                                                                          >  2:1273764/1‑101 (MQ=255)
                          cGGCCGGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGcc                                                                        <  1:604875/101‑1 (MQ=255)
                           ggCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCt                                                                       >  1:1004543/1‑101 (MQ=255)
                              cTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCg                                                                    <  2:1118771/101‑1 (MQ=255)
                                gTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTc                                                                  <  2:1688261/101‑1 (MQ=255)
                                  cAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTcgc                                                                <  1:36495/101‑1 (MQ=255)
                                   aGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTcgcg                                                               <  1:433086/101‑1 (MQ=255)
                                     ccGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGc                                                             >  1:4429154/1‑101 (MQ=255)
                                       ggCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCcg                                                           >  1:1492695/1‑101 (MQ=255)
                                       ggCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCcg                                                           <  1:300324/101‑1 (MQ=255)
                                       ggCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCcg                                                           <  1:2942734/101‑1 (MQ=255)
                                        gCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCcgc                                                          <  2:1595678/101‑1 (MQ=255)
                                           tGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGtt                                                       >  2:1717084/1‑101 (MQ=255)
                                                cAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCt                                                  <  2:1981753/101‑1 (MQ=255)
                                                cAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCt                                                  >  2:3639397/1‑101 (MQ=255)
                                                 aaCGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTc                                                 <  1:3587875/101‑1 (MQ=255)
                                                 aaCGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTc                                                 <  2:4466676/101‑1 (MQ=255)
                                                 aaCGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTc                                                 >  1:4655028/1‑101 (MQ=255)
                                                  aCGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCg                                                <  1:1602596/101‑1 (MQ=255)
                                                   cggcggCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGa                                               <  1:3904947/101‑1 (MQ=255)
                                                    ggcggcGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGaa                                              <  2:4631617/101‑1 (MQ=255)
                                                    ggcggcGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGaa                                              <  1:2184403/101‑1 (MQ=255)
                                                    ggcggcGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGaa                                              <  1:2597186/101‑1 (MQ=255)
                                                         cgcCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATcg                                         <  1:3306308/101‑1 (MQ=25)
                                                         cgcCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATcg                                         <  2:3489350/101‑1 (MQ=25)
                                                          gcCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATcgc                                        >  1:2016415/1‑101 (MQ=25)
                                                           ccGGTGGCG.CCTGCATGCGGAAGACCGCCGTCA.TCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATcgcc                                       >  1:1632225/1‑101 (MQ=21)
                                                           ccGGTGGCG.CCTGCATGCGGAAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATcgcc                                       <  2:4386057/101‑1 (MQ=25)
                                                            cGGTGGCG.CCTGCATGCGGAAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCACCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATcgccg                                      <  1:3100892/101‑1 (MQ=25)
                                                             ggTGGCG.CCTGCATGCGGAAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATcgccgc                                     <  1:2393775/101‑1 (MQ=25)
                                                             ggTGGCG.CCTGCATGCGGAAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATcgccgc                                     >  1:4302170/1‑101 (MQ=25)
                                                             ggTGGCG.CCTGCATGCGGAAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATcgccgc                                     <  2:2373067/101‑1 (MQ=25)
                                                              gTGGCG.CCTGCATGCGGAAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCa                                    <  1:1187955/101‑1 (MQ=25)
                                                              gTGGCG.CCTGCATGCGGAAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCa                                    >  1:4567167/1‑101 (MQ=25)
                                                              gTGGCG.CCTGCATGCGGAAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCa                                    >  2:3445105/1‑101 (MQ=25)
                                                               tGGCG.CCTGCATGCGGAAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCAt                                   >  1:2107733/1‑101 (MQ=21)
                                                               tGGCG.CCTGCATGCGGAAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCAt                                   >  1:3745693/1‑101 (MQ=21)
                                                                gggcgccTGCATGCGGAAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCAt                                   <  2:1446126/96‑1 (MQ=25)
                                                                 ggcgccTGCATGCGGAAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCAtt                                  <  1:1419742/97‑1 (MQ=25)
                                                                  gcgccTGCATGCGGAAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATtg                                 <  1:1719603/98‑1 (MQ=25)
                                                                    gccTGCATGCGGAAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATtgtg                               >  2:4517522/2‑101 (MQ=21)
                                                                     ccTGCATGCGGAAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGc                              <  2:4551534/101‑1 (MQ=25)
                                                                     ccTGCATGCGGAAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGc                              >  2:4094683/1‑101 (MQ=21)
                                                                      cTGCATGCGGAAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGCCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCt                             >  2:4332181/1‑101 (MQ=25)
                                                                       tGCATGCGGAAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTg                            <  1:1015694/101‑1 (MQ=25)
                                                                        gCATGCGGAAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTgg                           <  2:196101/101‑1 (MQ=25)
                                                                         cATGCGGAAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTggg                          <  1:2122383/101‑1 (MQ=25)
                                                                         cATGCGGAAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTggg                          <  1:71272/101‑1 (MQ=25)
                                                                           tgcggAAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTg                        <  1:228737/97‑1 (MQ=25)
                                                                           tgcggAAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTg                        <  1:989672/97‑1 (MQ=25)
                                                                           tgcggAAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTg                        <  2:1038345/97‑1 (MQ=25)
                                                                            gcggAAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGa                       <  1:1748034/98‑1 (MQ=25)
                                                                             cggAAGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGAc                      <  1:3470920/99‑1 (MQ=25)
                                                                                aaGACCGCCGTCA.GCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTg                   <  1:2556249/101‑1 (MQ=21)
                                                                                    accgccgtcagCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATc                >  1:1511911/11‑101 (MQ=21)
                                                                                     ccgccgtcagCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCGTGCGTCGCGGCCGCGTTTTACTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCa               >  1:3991465/10‑101 (MQ=21)
                                                                                     ccgccgtcagCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCa               <  2:3034640/92‑1 (MQ=21)
                                                                                     ccgccgtcagCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCa               <  1:3871472/92‑1 (MQ=21)
                                                                                      cgccgtcagCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCaa              <  1:2710458/93‑1 (MQ=21)
                                                                                      cgccgtcagCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCaa              <  2:1390319/93‑1 (MQ=21)
                                                                                         cgtcagCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCAAGCt           >  2:2967500/6‑101 (MQ=255)
                                                                                         cgtcagCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCAAGCt           >  1:4157367/6‑101 (MQ=255)
                                                                                           tcagCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCAAGCTgg         >  1:2745731/4‑101 (MQ=255)
                                                                                            cagCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCAAGCTGGt        <  1:887248/99‑1 (MQ=255)
                                                                                              gCTCCCGGAGGGGCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCAAGCTGGTTc      <  2:788778/101‑1 (MQ=18)
                                                                                              gCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCAAGCTGGTTc      <  2:564269/101‑1 (MQ=255)
                                                                                              gCTCCCGCAGGGGCTGCGCCTCGCCCACCAGCGCCTGAGTCGGGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGGGACCTGATCAAGCTGGTTc      >  2:428291/1‑101 (MQ=18)
                                                                                                tCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCAAGCTGGTTCAc    <  1:774339/101‑1 (MQ=255)
                                                                                                tCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCAAGCTGGTTCAc    <  1:3910959/101‑1 (MQ=255)
                                                                                                tCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCAAGCTGGTTCAc    >  1:1394161/1‑101 (MQ=255)
                                                                                                 cccGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCAAGCTGGTTCAcc   <  2:3335437/101‑1 (MQ=255)
                                                                                                  ccGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCAAGCTGGTTCAccg  <  1:4081823/101‑1 (MQ=255)
                                                                                                       |                                                                                               
AATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCGCCCTGCATGC.GAAGACCG.CGTAC.GCTCCCGCA.GGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCAAGCTGGTTCACCG  >  NC_014034/2304811‑2305005

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG