Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,308,282 +C 94.2% coding (515/1203 nt) RCAP_rcc02140 ← extracellular ligand‑binding receptor

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,308,2821.→C94.2% 18.3 ‑0.7 ‑1.3137coding (515/1203 nt)RCAP_rcc02140extracellular ligand‑binding receptor
Reads supporting (aligned to +/- strand):  new base (59/69):  ref base (5/3):  total (65/72)
Fisher's exact test strand distribution p-value = 4.75e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.54e-02

Only 100 of 147 total aligned reads displayed.

CCGTCAGCTGCAGCCCGCGTTTCGCAAGCGCCGCCTCCAGCGCCGCAAGCCCGGCCCGGCCGAAACTGTCGTCCTGATAAAGGAATGCCGATCTTCTGCTT.G.CCC.GGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATAGGAGGGGCGCAGGTTGAAAACATTGCGCAATTTCGGATCGCGCAGG  >  NC_014034/2308181‑2308377
                                                                                                       |                                                                                                
ccGTCAGCTGCAGCCCGCGTTTCGCAAGCGCCGCCTCCAGCGCCGCAAGCCCGGCCCGGCCGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTT.g                                                                                                   >  1:3706956/1‑101 (MQ=255)
  gTCAGCTGCAGCCCGCGTTTCGCAAGCGCCGCCTCCAGCGCCGCAAGCCCGGCCCGGCCGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTT.G.cc                                                                                                >  1:3363027/1‑101 (MQ=255)
  gTCAGCTGCAGCCCGCGTTTCGCAAGCGCCGCCTCCAGCGCCGCAAGCCCGGCCCGGCCGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTT.G.cc                                                                                                >  1:2557667/1‑101 (MQ=255)
   tCAGCTGCAGCCCGCGTTTCGCAAGCGCCGCCTCCAGCGCCGCAAGCCCGGCCCGGCCGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTT.G.ccc                                                                                               >  1:4123828/1‑101 (MQ=255)
      gCTGCAGCCCGCGTTTCGCAAGCGCCGCCTCCAGCGCCGCAAGCCCGGCCCGGCCGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTT.G.CCC.cgg                                                                                           >  1:1807534/1‑101 (MQ=255)
       cTGCAGCCCGCGTTTCGCAAGCGCCGCCTCCAGCGCCGCAAGCCCGGCCCGGCCGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTT.G.CCC.cggg                                                                                          >  1:3555670/1‑100 (MQ=255)
       cTGCAGCCCGCGTTTCGCAAGCGCCGCCTCCAGCGCCGCAAGCCCGGCCCGGCCGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTT.G.CCC.cggg                                                                                          >  1:3385887/1‑100 (MQ=255)
        tggcgCCAGCGTTTCGCAAGCGCCGCCTCCGGCGCCGCAAGCCCGGCCCGGCCGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTT.G.CCC.cgggt                                                                                         <  2:3933750/97‑3 (MQ=255)
        tGCAGCCCGCGTTTCGCAAGCGCCGCCTCCAGCGCCGCAAGCCCGGCCCGGCCGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTT.G.CCC.cgggt                                                                                         <  2:260101/101‑3 (MQ=255)
         gCAGCCCGCGTTTCGCAAGCGCCGCCTCCAGCGCCGCAAGCCCGGCCCGGCCGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTT.GCCCC.GGGtc                                                                                         >  1:1362882/1‑101 (MQ=255)
         gCAGCCCGCGTTTCGCAAGCGCCGCCTCCAGCGCCGCAAGCCCGGCCCGGCCGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTT.GCCCC.GGGtc                                                                                         <  2:2878268/101‑1 (MQ=255)
          cAGCCCGCGTTTCGCAAGCGCCGCCTCCAGCGCCGCAAGCCCGGCCCGGCCGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTT.GCCCC.GGGtct                                                                                        <  2:1756216/101‑1 (MQ=255)
           aGCCCGCGTTTCGCAAGCGCCGCCTCCAGCGCCGCAAGCCCGGCCCGGCCGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTT.GCCCC.GGGtctc                                                                                       <  2:2592225/101‑1 (MQ=255)
           aGCCCGCGTTTCGCAAGCGCCGCCTCCAGCGCCGCAAGCCCGGCCCGGCCGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTT.GCCCC.GGGtctc                                                                                       >  1:1170709/1‑101 (MQ=255)
           aGCCCGCGTTTCGCAAGCGCCGCCTCCAGCGCCGCAAGCCCGGCCCGGCCGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTT.GCCCC.GGGtctc                                                                                       >  1:4093410/1‑101 (MQ=255)
            gCCCGCGTTTCGCAAGCGCCGCCTCCAGCGCCGCAAGCCCGGCCCGGCCGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTT.GCCCC.GGGTCTCg                                                                                      <  2:362100/101‑1 (MQ=255)
              ccGCGTTTCGCAAGCGCCGCCTCCAGCGCCGCAAGCCCGGCCCGGCCGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTT.GCCCC.GGGTCTCGAc                                                                                    >  1:3041639/1‑101 (MQ=255)
                gcgTTTCTCAAGCTCCGCCTCCAGCGCCGCAAGCCCGGCCTGGCCGAAACTGTCGTTCTGATAAA.GAATGCCGATCTTCTGCTT.GCCCC.GGGTCTCGACCa                                                                                  <  1:4655306/101‑1 (MQ=255)
                gcgTTTCGCAAGCGCCGCCTCCAGCGCCGCAAGCCCGGCCCGGCCGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTT.GCCCC.GGGTCTCGACCa                                                                                  <  2:1275473/101‑1 (MQ=255)
                gcgTTGCGCAAGCGCCGCCTCCAGCGCCGCAAGCCCGGCCCGGCCGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTT.GCCCC.GGGTCTCGACCa                                                                                  <  2:819248/101‑1 (MQ=255)
                 cgTTTCGCAAGCGCCGCCTCCAGCGCCGCAAGCCCGGCCCGGCCGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTT.GTCCC.GGGTCTCGACCAg                                                                                 >  2:3441887/1‑101 (MQ=255)
                    ttCGCAAGCGCCGCCTCCAGCGCCGCAAGCCCGGCCCGGCCGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTT.GCCCC.GGGTCTCGACCAGATg                                                                              >  1:1577226/1‑101 (MQ=255)
                     tCGCAAGCGCCGCCTCCAGCGCCGCAAGCCCGGCCCGGCCGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTT.GCCCC.GGGTCTCGACCAGATGt                                                                             <  2:793079/101‑1 (MQ=255)
                      cGCAAGCGCCGCCTCCAGCGCCGCAAGCCCGGCCCGGCCGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTT.GCCCC.GGGTCTCGACCAGATGtt                                                                            <  2:2236112/101‑1 (MQ=255)
                        cAAGCGCCGCCTCCAGCGCCGCAAGCCCGGCCCGGCCGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTT.GCCCC.GGGTCTCGACCAGATGTTGc                                                                          <  2:3177124/101‑1 (MQ=255)
                        cAAGCGCCGCCTCCAGCGCCGCAAGCCCGGCCCGGCCGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTT.GCCCC.GGGTCTCGACCAGATGTTGc                                                                          >  1:437785/1‑101 (MQ=255)
                          aGCGCCGCCTCCAGCGCCGCAAGCCCGGCCCGGCCGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTT.GCCCC.GGGTCTCGACCAGATGTTGCAc                                                                        >  1:3406532/1‑101 (MQ=255)
                           gcgcCGCCTCCAGCGCCGCAAGCCCGGCCCGGCCGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTT.GCCCC.GGGTCTCGACCAGATGTTGCAcc                                                                       >  1:1092077/1‑101 (MQ=255)
                             gccgccTCCAGCGCCGCAAGCCCGGCCCGGCCGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTT.GCCCC.GGGTCTCGACCAGATGTTGCACCCa                                                                     <  2:3556215/101‑1 (MQ=255)
                              ccgccTCCAGCGCCGCAAGCCCGGCCCGGCCGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTT.GCCCC.GGGTCTCGACCAGATGTTGCACCCAg                                                                    <  1:2115668/101‑1 (MQ=255)
                               cgccTCCAGCTCCGCAAGCCCGGCCCGGCCGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTT.GCCCC.GGGTCTCGACCAGATGTTGCACCCAgg                                                                   <  2:3054036/101‑1 (MQ=255)
                               ccgatctaGCGCCGCAAGCCCGGCCCGGCCGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTT.GCCCC.GGGTCTCGACCAGATGTTGCACCCAgg                                                                   <  2:3910908/94‑1 (MQ=255)
                                   tCCAGCGCCGCAAGCCCGGCCCGGCCGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTT.GCCCC.GGGTCTCGACCAGATGTTGCACCCAggcctc                                                               <  2:1170617/101‑1 (MQ=255)
                                    ccAGCGCCGCAAGCCCGGCCCGGCCGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTT.GCCCC.GGGTCTCGACCAGATGTTGCACCCAggcctcg                                                              >  1:2827891/1‑101 (MQ=255)
                                      aGCGCCGCAAGCCCGGCCCGGCCGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTT.GCCCC.GGGTCTCGACCAGATGTTGCACCCAggagatcgg                                                            >  1:3911193/1‑94 (MQ=255)
                                       gcgcCGCAAGCCCGGCCCGGCCGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTT.GCCCC.GGGTCTCGACCAGATGTTGCACCCAggcctcggcc                                                           <  2:1689488/101‑1 (MQ=255)
                                        cgccgcAAGCCCGGCCCGGCCGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTT.GCCCC.GGGTCTCGACCAGATGTTGCACCCAggcctcggcct                                                          <  2:1256957/101‑1 (MQ=255)
                                        ccccgcAAGCCCGGCCCGGCCGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTT.GCCCC.GGGTCTCGACCAGATGTTGCACCCAggcctcggcct                                                          <  1:3961049/99‑1 (MQ=255)
                                         gccgcAAGCCCGGCCCGGCCGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTT.GCCCC.GGGTCTCGACCAGATGTTGCACCCAggcctcggcctc                                                         >  1:3393313/1‑101 (MQ=255)
                                         gccgcAAGCCCGGCCCGGCCGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTT.GCCCC.GGGTCTCGACCAGATGTTGCACCCAggcctcggcctc                                                         >  1:3144035/1‑101 (MQ=255)
                                         gccgcAAGCCCGGCCCGGCCGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTT.GCCCC.GGGTCTCGACCAGATGTTGCACCCAggcctcggcctc                                                         >  1:2942380/1‑101 (MQ=255)
                                          ccgcAAGCCCGGCCCGGCCGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTT.GCCCC.GGGTCTCGACCAGATGTTGCACCCAGgcctcggcctcg                                                        <  2:806268/101‑1 (MQ=255)
                                            gcAAGCCCGGCCCGGCCGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTT.GCCCC.GGGTCTCGACCAGATGTTGCACCCAGGCctcggcctcggc                                                      >  1:1892009/1‑101 (MQ=255)
                                              aaGCCCGGCCCGGCCGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTT.GCCCC.GGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGgcgc                                                    >  1:3855300/1‑101 (MQ=255)
                                               aGCCCGGCCCGGCCGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTT.GCCCC.GGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGcc                                                   >  1:2468750/1‑101 (MQ=255)
                                               aGCCCGGCCCGGCCGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTT.GCCCC.CGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGcc                                                   <  2:2384505/101‑1 (MQ=255)
                                                 cccggcccggCCGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTT.GCCCC.GGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCAt                                                 <  2:860057/101‑1 (MQ=255)
                                                 cccggcccggCCGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTT.GCCCC.GGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCAt                                                 >  1:2573249/1‑101 (MQ=255)
                                                    ggcccggccGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTT.GCCCC.GGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATagg                                              <  2:3065446/101‑1 (MQ=255)
                                                    ggcccggccGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTT.GCCCC.GGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATagg                                              <  2:2351768/101‑1 (MQ=255)
                                                     gcccggccGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTT.GCCCC.GGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATagga                                             <  2:3145971/101‑1 (MQ=255)
                                                     gcccggccGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTT.GCCCC.GGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATagga                                             >  1:2106800/1‑101 (MQ=255)
                                                     gcccggccGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTT.GCCCC.GGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATagga                                             <  2:2074330/101‑1 (MQ=255)
                                                     gcccggccGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTT.GCCCC.GGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATagga                                             <  2:3732910/101‑1 (MQ=255)
                                                       ccggccGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTT.GCCCC.GGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATaggagg                                           <  2:1774172/101‑1 (MQ=255)
                                                       ccggccGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTT.G.CCCTGGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATaggagg                                           <  2:618932/101‑1 (MQ=255)
                                                        cggccGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTT.GCCCC.GGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATAGGAggg                                          <  1:912449/101‑1 (MQ=255)
                                                        cggccGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTT.GCCCC.GGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATAGGAggg                                          >  1:2893526/1‑101 (MQ=255)
                                                        cggccGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTT.GCCCC.GGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATAGGAggg                                          >  1:2766618/1‑101 (MQ=255)
                                                         ggccGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTT.GCCCC.GGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATAGGAgggg                                         <  2:2829892/101‑1 (MQ=255)
                                                         ggccGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTT.GCCCC.GGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATAGGAgggg                                         >  1:1292391/1‑101 (MQ=255)
                                                            cGAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTT.GCCCC.GGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATAGGAGGGgcgc                                      >  1:2744906/1‑101 (MQ=255)
                                                             gAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTT.GCCCC.GGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATAGGAGGGGCGCa                                     <  2:114938/101‑1 (MQ=255)
                                                             gAAACTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTT.GCCCC.GGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATAGGAGGGGCGCa                                     >  1:3027849/1‑101 (MQ=255)
                                                              aaaCTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTT.GCCCC.GGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATAGGAGGGGCGCAg                                    >  1:3584045/1‑101 (MQ=255)
                                                               aaCTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTT.GCCCC.GGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATAGGAGGGGCGCAgg                                   <  1:2084132/101‑1 (MQ=255)
                                                                aCTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTT.GCCCC.GGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATAGGAGGGGCGCAGGt                                  <  2:4311239/101‑1 (MQ=255)
                                                                aCTGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTT.GCCCC.GGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATAGGAGGGGCGCAGGt                                  >  1:535753/1‑101 (MQ=255)
                                                                  tGTCGTCCTGATAAA.GAATGCCGATCTTCTGCTT.GCCCC.GGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATAGGAGGGGCGCAGGTTg                                >  1:815165/1‑101 (MQ=255)
                                                                   gtcgtcCTGATAAA.GAATGCCGATCTTCTGCTT.GCCCC.GGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATAGGAGGGGCGCAGGTTGa                               >  1:1558199/1‑101 (MQ=255)
                                                                   gtcgtcCTGATAAA.GAATGCCGATCTTCTGCTT.GCCCC.GGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATAGGAGGGGCGCAGGTTGa                               >  1:2298239/1‑101 (MQ=255)
                                                                     cgtcCTGATAAA.GAATGCCGATCTTCTGCTT.GCCCC.GGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATAGGAGGGGCGCAGGTTGaaa                             >  1:4422007/1‑101 (MQ=255)
                                                                      gtcCTGATAAA.GAATGCCGATCTTCTGCTT.GCCCC.GGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATAGGAGGGGCGCAGGTTGaaaa                            >  1:2197119/1‑101 (MQ=255)
                                                                       tcCTGATAAA.GAATGCCGATCTTCTGCTT.GCCCC.GGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATAGGAGGGGCGCAGGTTGAAAAc                           >  1:4329805/1‑101 (MQ=255)
                                                                         cTGATAAA.GAATGCCGATCTTCTGCTT.GCCCC.GGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATAGGAGGGGCGCAGGTTGAAAACAt                         >  1:622407/1‑101 (MQ=255)
                                                                            aTAAA.GAATGCCGATCTTCTGCTT.GCCCC.GGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATAGGAGGGGCGCAGGTTGAAAACATTgc                      >  1:2451775/1‑101 (MQ=255)
                                                                               aaagAATGCCGATCTTCTGCTT.GCCCC.GGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATAGGAGGGGCGCAGGTTGAAAACATTgcgc                    >  1:2861180/4‑101 (MQ=255)
                                                                               aaagAATGCCGATCTTCTGCTT.GCCCC.GGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATAGGAGGGGCGCAGGTTGAAAACATTgcgc                    >  1:1366721/4‑101 (MQ=255)
                                                                               aaagAATGCCGATCTTCTGCTT.GCCCC.GGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATAGGAGGGGCGCAGGTTGAAAACATTgcgc                    <  2:552260/98‑1 (MQ=255)
                                                                                  gAATGCCGATCTTCTGCTT.GCCCC.GGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATAGGAGGGGCGCAGGTTGAAAACATTGCGCAAt                 <  2:616052/101‑1 (MQ=255)
                                                                                  gAATGCCGATCTTCTGCTT.GCCCC.GGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATAGGAGGGGCGCAGGTTGAAAACATTGCGCAAt                 <  2:1774390/101‑1 (MQ=255)
                                                                                   aaTGCCGATCTTCTGCTT.GCCCC.GGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATAGGAGGGGCGCAGGTTGAAAACATTGCGCAAtt                <  2:437748/101‑1 (MQ=255)
                                                                                   aaTGCCGATCTTCTGCTT.GCCCC.GGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATAGGAGGGGCGCAGGTTGAAAACATTGCGCAAtt                <  2:3555412/101‑1 (MQ=255)
                                                                                   aaTGCCGATCTTCTGCTT.GCCCC.GGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATAGGAGGGGCGCAGGTTGAAAACATTGCGCAAtt                >  1:1969996/1‑101 (MQ=255)
                                                                                      gCCGATCTTCTGCTTGG.CCC.GGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATAGGAGGGGCGCAGGTTGAAAACATTGCGCAATTTCg             >  1:4072765/1‑101 (MQ=255)
                                                                                      gCCGATCTTCTGCTT.GCCCC.GGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATAGGAGGGGCGCAGGTTGAAAACATTGCGCAATTTCg             <  2:460610/101‑1 (MQ=255)
                                                                                      gCCGATCTTCTGCTT.GCCCC.GGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATAGGAGGGGCGCAGGTTGAAAACATTGCGCAATTTCg             >  1:1079266/1‑101 (MQ=255)
                                                                                      gCCGATCTTCTGCTT.GCCCC.GGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATAGGAGGGGCGCAGGTTGAAAACATTGCGCAATTTCg             <  2:2215567/101‑1 (MQ=255)
                                                                                         gATCTTCTGCTT.GCCCC.GGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATAGGAGGGGCGCAGGTTGAAAACATTGCGCAATTTCGGAt          <  2:2851278/101‑1 (MQ=255)
                                                                                          aTCTTCTGCTT.GCCCC.GGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATAGGAGGGGCGCAGGTTGAAAACATTGCGCAATTTCGGaac         >  1:1084171/1‑99 (MQ=255)
                                                                                           tcttctGCTT.GCCCC.GGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATAGGAGGGGCGCAGGTTGAAAACATTGCGCAATTTCGGATcg        <  2:2801573/101‑1 (MQ=255)
                                                                                           tcttctGCTT.GCCCC.GGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATAGGAGGGGCGCAGGTTGAAAACATTGCGCAATTTCGGATcg        <  1:4372590/101‑1 (MQ=255)
                                                                                            cttctGCTT.GCCCC.GGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATAGGAGGGGCGCAGGTTGAAAACATTGCGCAATTTCGGATcgc       >  1:1989590/1‑101 (MQ=255)
                                                                                            cttctGCTT.GCCCC.GGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATAGGAGGGGCGCAGGTTGAAAACATTGCGCAATTTCGGATcgc       >  1:831326/1‑101 (MQ=255)
                                                                                             ttctGCTT.GCCCC.GGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATAGGAGGGGCGCAGGTTGAAAACATTGCGCAATTTCGGATcgcg      >  1:3613649/1‑101 (MQ=255)
                                                                                              tctGCTT.GCCCC.GGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATAGGAGGGGCGCAGGTTGAAAACATTGCGCAATTTCGGATCgcgc     >  1:2324402/1‑101 (MQ=255)
                                                                                               ctGCTT.GCCCC.GGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATAGGAGGGGCGCAGGTTGAAAACATTGCGCAATTTCGGATCGCGCa    >  1:1803654/1‑101 (MQ=255)
                                                                                               ctGCTT.GCCCC.GGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATAGGAGGGGCGCAGGTTGAAAACATTGCGCAATTTCGGATCGCGCa    >  1:3526864/1‑101 (MQ=255)
                                                                                                tGCTT.GCCCC.GGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATAGGAGGGGCGCAGGTTGAAAACATTGCGCAATTTCGGATCGCGCAg   <  2:3855015/101‑1 (MQ=255)
                                                                                                 gCTT.GCCCC.GGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATAGGAGGGGCGCAGGTTGAAAACATTGCGCAATTTCGGATCGCGCAgg  <  2:3406278/101‑1 (MQ=255)
                                                                                                       |                                                                                                
CCGTCAGCTGCAGCCCGCGTTTCGCAAGCGCCGCCTCCAGCGCCGCAAGCCCGGCCCGGCCGAAACTGTCGTCCTGATAAAGGAATGCCGATCTTCTGCTT.G.CCC.GGGTCTCGACCAGATGTTGCACCCAGGCCTCGGCCTCGGCGCCATAGGAGGGGCGCAGGTTGAAAACATTGCGCAATTTCGGATCGCGCAGG  >  NC_014034/2308181‑2308377

Base quality scores:  ATCG  < 3 ≤  ATCG  < 24 ≤  ATCG  < 31 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG