Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,420,305 +G 2.1% intergenic (‑99/+93) RCAP_rcc02245 ← / ← cysE2 major membrane protein I/serine O‑acetyltransferase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,420,3050.→G 2.1% 5.0 ‑1.2 ‑0.6189intergenic (‑99/+93)RCAP_rcc02245/cysE2major membrane protein I/serine O‑acetyltransferase
Reads supporting (aligned to +/- strand):  new base (3/1):  ref base (77/108):  total (80/109)
Fisher's exact test strand distribution p-value = 3.13e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.60e-01

Only 100 of 195 total aligned reads displayed.

GCCAGCGCGGCAGTGATCGTGGGCCAGGATACGGCGCCCGTCTTCGTGGCATTGGCAAAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAGTTTGACACCGGTTCGGTCATTCACGTTCTCCTTCTTGGGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCAGGTTGAAAATGTGTTCTG  >  NC_014034/2420209‑2420395
                                                                                                                                                                                           
gccagcGCGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTc                                                                                 <  2:2594979/101‑1 (MQ=255)
gccagcGCGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTc                                                                                 <  1:2508007/101‑1 (MQ=255)
  cagcGCGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCAt                                                                               >  1:555853/1‑101 (MQ=255)
   agcGCGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCAtt                                                                              >  2:86120/1‑101 (MQ=255)
   agcGCGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCAtt                                                                              >  1:1285018/1‑101 (MQ=255)
   agcGCGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCAtt                                                                              >  2:1690337/1‑101 (MQ=255)
    gcgcGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTc                                                                             <  1:4289426/101‑1 (MQ=255)
       gcggcgTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCAc                                                                           <  1:3856316/96‑1 (MQ=255)
       gcggcgTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCAc                                                                           <  1:48356/96‑1 (MQ=255)
       gcggcgTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCAc                                                                           <  2:1000774/96‑1 (MQ=255)
        cggcgTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACg                                                                          >  2:3037795/5‑101 (MQ=255)
         ggcgTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGt                                                                         <  2:523898/98‑1 (MQ=255)
          gcgTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGtt                                                                        <  1:4636274/99‑1 (MQ=255)
          gcgTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGtt                                                                        <  2:698452/99‑1 (MQ=255)
          gcgTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCCTCCAGCGCCAG.TTGACGCGGGTT.GGTCATTCACGtt                                                                        >  1:4195146/3‑101 (MQ=255)
           cgTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTtc                                                                       <  1:1205271/100‑1 (MQ=255)
             tGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTtctc                                                                     <  2:1544350/101‑1 (MQ=255)
              gATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTcc                                                                    >  1:3511158/1‑101 (MQ=255)
              gATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTcc                                                                    >  1:2671493/1‑101 (MQ=255)
              gATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTcc                                                                    >  1:3697283/1‑101 (MQ=255)
               aTCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCct                                                                   <  1:1362765/101‑1 (MQ=255)
               aTCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCct                                                                   <  2:3563649/101‑1 (MQ=255)
                tCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCctt                                                                  <  2:2176101/101‑1 (MQ=255)
                 cGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCcttc                                                                 <  2:51140/101‑1 (MQ=255)
                  gTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCcttct                                                                >  2:3801326/1‑101 (MQ=255)
                  gTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCcttct                                                                >  1:20801/1‑101 (MQ=255)
                     gggcAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTTg                                                              >  1:3590029/4‑101 (MQ=255)
                       gcAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTTggg                                                            <  2:4149930/100‑1 (MQ=255)
                          aggatcGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTTgggtg                                                          <  2:4004108/96‑3 (MQ=255)
                          aggatcGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTTgggtg                                                          >  2:3332567/6‑99 (MQ=255)
                          aggatcGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCCCCTTCTTgggtg                                                          <  1:2525506/96‑3 (MQ=255)
                             atcGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAg                                                      <  2:339229/99‑1 (MQ=255)
                              tcGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGa                                                     <  1:233974/100‑1 (MQ=255)
                               cGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGaa                                                    <  1:3655740/101‑1 (MQ=255)
                               cGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGaa                                                    <  2:1959816/101‑1 (MQ=255)
                                 ggcgccGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGaaa                                                   >  1:3816291/5‑101 (MQ=255)
                                 ggcgccGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGaaa                                                   <  1:3332816/97‑1 (MQ=255)
                                 ggcgccGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGaaa                                                   >  1:1783317/5‑101 (MQ=255)
                                  gcgccGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGaaaa                                                  <  2:3843529/98‑1 (MQ=255)
                                  gcgccGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGaaaa                                                  <  2:3482225/98‑1 (MQ=255)
                                    gccGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAAcc                                                <  2:3277155/100‑1 (MQ=255)
                                    gccGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAAcc                                                >  2:3834594/2‑101 (MQ=255)
                                       gTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATc                                             <  2:2807066/101‑1 (MQ=255)
                                        tCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATcc                                            <  1:3383602/101‑1 (MQ=255)
                                        tCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGACAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATcc                                            >  1:3764760/1‑101 (MQ=255)
                                         cTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATccc                                           <  2:3698111/101‑1 (MQ=255)
                                          ttCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCcg                                          >  1:1988794/1‑101 (MQ=255)
                                          ttCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCcg                                          >  2:606515/1‑101 (MQ=255)
                                          ttCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCcg                                          <  2:4210670/101‑1 (MQ=255)
                                           tCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCcgc                                         >  2:4326848/1‑101 (MQ=255)
                                            cGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCcgcg                                        >  1:1545975/1‑101 (MQ=255)
                                              tGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGa                                      <  2:1932075/101‑1 (MQ=255)
                                              tGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGa                                      <  2:26617/101‑1 (MQ=255)
                                                gCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGAcg                                    >  2:2312577/1‑101 (MQ=255)
                                                gCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGAcg                                    <  2:2829452/101‑1 (MQ=255)
                                                gCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGACATTCACGTTCTCCTTCTT.GGGCGCAGAAAACCATCCCGCGGAcg                                    >  2:2300922/1‑101 (MQ=255)
                                                gCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.CTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGAcg                                    >  1:742637/1‑101 (MQ=255)
                                                gCATTGGC.AAGCGGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGAcg                                    <  1:1710533/101‑1 (MQ=255)
                                                 cATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTCAC.GCGGTTCGGTCATTCACGGTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGAcgc                                   >  2:2374447/1‑101 (MQ=255)
                                                  aTTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGAcgcg                                  >  1:2876951/1‑101 (MQ=255)
                                                     tggcaaGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGcgcc                                <  1:4569852/97‑1 (MQ=255)
                                                       gcaaGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGcgccgc                              >  1:1602755/3‑101 (MQ=255)
                                                         aaGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCgg                            >  1:195050/1‑101 (MQ=255)
                                                         aaGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCgg                            >  2:547412/1‑101 (MQ=255)
                                                          aGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCggg                           <  2:636566/101‑1 (MQ=255)
                                                          aGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCggg                           <  1:2150919/101‑1 (MQ=255)
                                                           gCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGGCGCGCCGCgggg                          >  1:2925398/1‑100 (MQ=255)
                                                           gCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGa                          <  1:1041571/101‑1 (MQ=255)
                                                             tGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGAtt                        <  2:3926659/101‑1 (MQ=255)
                                                             tGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGAtt                        <  1:4333075/101‑1 (MQ=255)
                                                               gcgggcggCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTgg                      <  1:298017/101‑1 (MQ=255)
                                                               gcgggcggCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTgg                      <  2:3697008/101‑1 (MQ=255)
                                                                 gggcggCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCa                    >  2:1832230/1‑101 (MQ=255)
                                                                 gggcggCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCa                    <  2:1284925/101‑1 (MQ=255)
                                                                 gggcggCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCa                    <  1:3951756/101‑1 (MQ=255)
                                                                 gggcggCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCa                    <  1:3496980/101‑1 (MQ=255)
                                                                  ggcggcACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCAg                   <  1:2994019/101‑1 (MQ=255)
                                                                    cggcACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCAGGt                 <  2:1261718/101‑1 (MQ=255)
                                                                     ggcACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCAGGtt                <  2:1183395/101‑1 (MQ=255)
                                                                      gcACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCAGGTTg               >  1:3089701/1‑101 (MQ=255)
                                                                      gcACGGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCAGGTTg               <  2:2616958/101‑1 (MQ=255)
                                                                       cACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCAGGTTGa              <  1:86364/101‑1 (MQ=255)
                                                                        ccTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCAGGTTGaa             <  2:1645308/100‑1 (MQ=255)
                                                                         cTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCAGGTTGtaa            >  1:3028593/1‑101 (MQ=255)
                                                                         cTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCAGGTTGaaa            <  1:2374609/101‑1 (MQ=255)
                                                                          tGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCAGGTTGaaaa           >  2:741094/1‑101 (MQ=255)
                                                                          tGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCAGGTTGaaaa           >  1:3632016/1‑101 (MQ=255)
                                                                          tGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCAGGTTGaaaa           >  1:3785961/1‑101 (MQ=255)
                                                                          tGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCAGGTTGaaaa           <  2:1393375/101‑1 (MQ=255)
                                                                            tCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCAGGTTGAAAAtg         <  2:2451780/101‑1 (MQ=255)
                                                                             cGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCAGGTTGAAAAtgg        >  1:3663677/1‑100 (MQ=255)
                                                                              gCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCAGGTTGAAAAtgtg       <  2:4591738/101‑1 (MQ=255)
                                                                               cccAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCAGGTTGAAAATgtgt      >  1:2602541/1‑101 (MQ=255)
                                                                                 cAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCAGGTTGAAAATGTGTTc    >  2:3666433/1‑101 (MQ=255)
                                                                                 cAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCAGGTTGAAAATGTGTTc    <  2:4345147/101‑1 (MQ=255)
                                                                                 cAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCAGGTTGAAAATGTGTTc    >  1:2684088/1‑101 (MQ=255)
                                                                                  aGCGCCAG.TTGACG.CGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCNGGTTGAAAATGTGGTCt   >  2:1987435/1‑101 (MQ=255)
                                                                                   gcgcCAG.TTGACG.CGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCAGGTTGAAAATGTGTTCtg  <  1:1913940/101‑1 (MQ=255)
                                                                                   gcgcCAG.TTGACG.CGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCAGGTTGAAAATGTGTTCtg  >  1:1406536/1‑101 (MQ=255)
                                                                                   gcgcCAG.TTGACG.CGGTTCGGTCATTCACGTTCTCCTTCTT.GGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCAGGGTGAAAATGTGTTCtg  >  1:226427/1‑101 (MQ=255)
                                                                                                                                                                                           
GCCAGCGCGGCAGTGATCGTGGGCCAGGATACGGCGCCCGTCTTCGTGGCATTGGCAAAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAGTTTGACACCGGTTCGGTCATTCACGTTCTCCTTCTTGGGGTGCAGAAAACCATCCCGCGGACGCGCCGCGGGATTGGCAGGTTGAAAATGTGTTCTG  >  NC_014034/2420209‑2420395

Base quality scores:  ATCG  < 3 ≤  ATCG  < 21 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 40 ≤  ATCG