Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,494,061 +A 86.6% intergenic (+109/‑133) RCAP_rcc02319 → / → RCAP_rcc02320 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,494,0611.→A86.6% 36.5 ‑0.3 ‑0.197intergenic (+109/‑133)RCAP_rcc02319/RCAP_rcc02320hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (51/33):  ref base (9/4):  total (60/37)
Fisher's exact test strand distribution p-value = 7.61e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.72e-01

Only 100 of 123 total aligned reads displayed.

GCTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AG.CAGA.AGGGGGGCAAGCG.CCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAG  >  NC_014034/2493961‑2494151
                                                                                                        |                                                                                              
gcTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..g                                                                                                 >  2:2159991/1‑101 (MQ=255)
 cTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGG..G.a                                                                                               >  1:4349045/1‑101 (MQ=18)
   gCCCCCCGTCCGCAAGCGGACTCCCCCCGGGCTATTTGCAGCAAGATGCAACCGAATGCCTTTCATCTTGCCCGCAATATCCCGGGGGGAGAA.GGGG..G.aag                                                                                             >  1:124217/1‑99 (MQ=18)
   gCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aag                                                                                             <  1:704842/101‑3 (MQ=255)
   gCCCCCCGTCCGCAAGCGGAATCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.GGGC..G.aac                                                                                             >  2:2183415/1‑99 (MQ=18)
    cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcc                                                                                           <  1:434851/101‑5 (MQ=255)
    cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcc                                                                                           <  1:589397/101‑5 (MQ=255)
    cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcc                                                                                           >  1:422015/1‑97 (MQ=255)
    cccccCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagc                                                                                            <  1:198496/101‑4 (MQ=255)
     ccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcca                                                                                          <  2:3670665/101‑6 (MQ=255)
       cccgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccag                                                                                         <  1:509831/97‑7 (MQ=255)
       cccgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..G.aagccag                                                                                         >  1:2985216/5‑95 (MQ=18)
                aaGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGCa                                                                               <  1:814009/101‑1 (MQ=18)
                 aGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGCag                                                                              <  2:1264520/101‑2 (MQ=18)
                   cGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGCagcg                                                                            >  1:2943247/1‑98 (MQ=18)
                    ggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGCagcgc                                                                           >  1:798133/1‑97 (MQ=17)
                      aCTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGG..GAAG.CCAG.AGGGGGGC.AGCG.ccc                                                                       >  2:4255159/1‑101 (MQ=17)
                      aCTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCG.ccc                                                                       >  2:2256315/1‑101 (MQ=18)
                        tCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAG..AGGGGGGC.AGCG.ccc.cc                                                                    <  2:1714876/101‑1 (MQ=17)
                        tCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCG.ccc.cc                                                                    >  1:2173295/1‑101 (MQ=18)
                          cccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCG.ccc.ccct                                                                  <  2:2243612/101‑3 (MQ=17)
                           ccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCG.ccc.ccctg                                                                 <  2:2984987/101‑4 (MQ=17)
                           ccccGGGATATCTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCG.ccc.ccctg                                                                 >  1:2729047/1‑98 (MQ=17)
                              cGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AA.GACAGAGGGGGGC.AGCG.ccc.ccctgacc                                                              >  1:1814513/1‑95 (MQ=17)
                               gggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGCAGCAC.CCC.CCTGcccc                                                              >  2:344576/1‑101 (MQ=17)
                                ggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCA.AGAGGGGGCAGCG.CCC.CCTGCCCcg                                                             >  2:4133702/1‑101 (MQ=2)
                                ggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCA.AGAGGGGGCAGCG.CCC.CCTGCCCcg                                                             <  1:2338829/101‑1 (MQ=0)
                                ggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCA.AGAGGGGGCAGCG.CCC.CCTGCCCcg                                                             >  1:1282178/1‑101 (MQ=2)
                                ggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCcg                                                             >  2:4256564/1‑101 (MQ=17)
                                 gATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCA.AGAGGGGGCAGCG.CCC.CCTGCCCcgc                                                            >  2:4290240/1‑100 (MQ=2)
                                  atatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCA.AGAGGGGGCAGCG.CCC.CCTGCCCcgcc                                                           >  1:3869648/1‑99 (MQ=0)
                                  atatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCA.AGAGGGGGCAGCG.CCC.CCTGCCCcgcc                                                           <  1:1127028/101‑3 (MQ=2)
                                  atatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGCGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCcggc                                                           >  2:477874/1‑101 (MQ=17)
                                   tatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCA.AGAGGGGGCAGCG.CCC.CCTGCCCcgccg                                                          <  2:2091493/101‑1 (MQ=2)
                                   tatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGT..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCcggcg                                                          >  1:1457259/1‑101 (MQ=17)
                                   tatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGACCcggcg                                                          >  2:1611164/1‑101 (MQ=17)
                                      ttGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGCAGCGG.CCC.CCGGCCCCGGCGGTc                                                       >  2:714330/1‑101 (MQ=18)
                                      ttGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTc                                                       <  1:1467624/101‑1 (MQ=17)
                                       tGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTcc                                                      >  1:3926664/1‑101 (MQ=18)
                                       tGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTcc                                                      <  2:1703597/101‑1 (MQ=17)
                                        gcagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCg                                                     >  1:1658069/1‑101 (MQ=17)
                                        gcagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAC.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCg                                                     >  1:4037011/1‑101 (MQ=18)
                                         cagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGt                                                    >  2:4340374/1‑101 (MQ=18)
                                         cagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCG.CCC.CCCGCCCCCGCGGGcccgg                                                   >  1:4092509/1‑98 (MQ=17)
                                         cagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGCGAA.TGGC..GAAG.CCAC.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGt                                                    >  1:2256617/1‑101 (MQ=18)
                                           gcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAG.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTgg                                                  >  2:294835/1‑101 (MQ=18)
                                           gcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTgg                                                  <  2:2629435/101‑1 (MQ=17)
                                            caAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTggc                                                 >  2:2049065/1‑101 (MQ=18)
                                              aGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTggcgg                                               <  2:3823272/101‑1 (MQ=17)
                                              aGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGG.AAGCGCCCC.CCTGCCCCGGCGGTCCGGggcgg                                               >  1:1331463/1‑101 (MQ=17)
                                                 tGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGgcgc                                            >  1:4604971/1‑101 (MQ=17)
                                                     ccgggctGCCTTTAATCTTG.CCAAAACTTGCCGGGGGGGGAAGGGGC..GAAG.CGGG.GGGGGGCCACCCC.CCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTg                                        <  2:2728849/95‑1 (MQ=255)
                                                        ggATGCCTTTCATCTTTCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcg                                     <  1:3085165/101‑1 (MQ=17)
                                                         gATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgg                                    >  1:1199947/1‑101 (MQ=17)
                                                           tGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggc                                  >  2:3668466/1‑101 (MQ=18)
                                                            gCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggcg                                 >  1:3532805/1‑101 (MQ=18)
                                                              cTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggcggg                               >  1:4153737/1‑101 (MQ=18)
                                                               tttCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCgggg                              <  1:2721423/101‑1 (MQ=17)
                                                               tttCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCgggg                              <  2:3939830/101‑1 (MQ=17)
                                                               tttCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCgggg                              <  1:402025/101‑1 (MQ=17)
                                                                ttCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCggggg                             >  2:2317350/1‑101 (MQ=18)
                                                                 tCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGgc                            <  2:3763269/101‑1 (MQ=18)
                                                                  cATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AA.GCCC.GCGGGGGCCAGCGCCCC.CCTGCCCCGCCGGTCCGTGGCGGGGCCTTGACGGGCggggggg                           >  2:349165/1‑99 (MQ=12)
                                                                  cATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGG..GAAG.TCAA.GGGGGGGCAGGCC.CCC.CCTAGCCAGGGCGTCCGGGGCGGGCCCTGGAGGGGGggggggg                           >  1:2574823/1‑99 (MQ=9)
                                                                   aTCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGgcgc                          <  1:31269/101‑1 (MQ=18)
                                                                    tCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGcgcg                         >  1:3698092/1‑101 (MQ=18)
                                                                     cTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGGGCgg                        >  1:2097304/1‑101 (MQ=18)
                                                                     cTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCgg                        >  2:2565296/1‑101 (MQ=18)
                                                                       tGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGc                      <  2:2943018/101‑1 (MQ=18)
                                                                       tGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGAGGCCCGTGGCGGCGCCTTGACGAGCGGGGGCGCGGGc                      >  2:1911068/1‑101 (MQ=17)
                                                                       tGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AA.GCCA.GAGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCa                     >  1:3546810/1‑101 (MQ=21)
                                                                        gCCCGAAATATCCCGGGGGGTGGA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCa                     >  2:1332826/1‑101 (MQ=18)
                                                                          ccGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCAtt                   <  1:2906827/101‑1 (MQ=18)
                                                                            gAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCagatc                 >  2:2489016/1‑97 (MQ=18)
                                                                            gAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTaa                 <  1:2256477/101‑1 (MQ=18)
                                                                            gAAATATCCCGGGGGGGGAA.TGGC..GAAG.CCAG.AGGGGGGCATCGC.CCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGTGGGCGTGGGCATTaa                 >  2:449851/1‑101 (MQ=21)
                                                                            gAAATATCCCGGGGGGGGAA.AGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTaa                 >  2:2097177/1‑101 (MQ=18)
                                                                             aaaTATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAAc                >  1:1967380/1‑101 (MQ=18)
                                                                             aaaTATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAAc                <  1:4330329/101‑1 (MQ=18)
                                                                              aaTATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAAcc               >  2:584359/1‑101 (MQ=21)
                                                                                tgaggcgtggggTGAA.TGGC..GAAG.CCAG.AGGGTGGCAGCAC.CCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGa             <  1:99495/93‑1 (MQ=19)
                                                                                tatCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGa             >  2:2484677/1‑101 (MQ=21)
                                                                                tatCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGa             <  2:798059/101‑1 (MQ=21)
                                                                                 atCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGGATTAACCGaa            >  1:2619658/1‑101 (MQ=21)
                                                                                  tCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGaaa           <  2:14019/101‑1 (MQ=21)
                                                                                  tCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGaaa           <  1:867912/101‑1 (MQ=21)
                                                                                   cccGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAAt          >  1:890800/1‑101 (MQ=21)
                                                                                   cccGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAAt          <  2:4246277/101‑1 (MQ=21)
                                                                                     cGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTg        >  2:815951/1‑101 (MQ=25)
                                                                                     cGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTg        <  1:3893464/101‑1 (MQ=25)
                                                                                      ggggggTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCG.CCCACCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGc       <  1:3245406/101‑1 (MQ=25)
                                                                                       gggggTGAA.TGGC..GAAGCCAG..AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCa      >  1:2084434/1‑101 (MQ=25)
                                                                                       gggggTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCa      >  1:4286504/1‑101 (MQ=25)
                                                                                       gggggTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCa      <  1:143055/101‑1 (MQ=25)
                                                                                        ggggTGAA.TGGC..GAAGCCAG..AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCaa     >  2:39123/1‑101 (MQ=25)
                                                                                        ggggTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCaa     >  2:1707501/1‑101 (MQ=25)
                                                                                         gggTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCaaa    >  1:454746/1‑100 (MQ=25)
                                                                                          ggTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGACCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCAATAACCGAAATTGCAACa   >  2:2854310/1‑101 (MQ=21)
                                                                                           gTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAg  >  2:4632422/1‑101 (MQ=25)
                                                                                           gTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAg  <  1:3579218/101‑1 (MQ=25)
                                                                                                        |                                                                                              
GCTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AG.CAGA.AGGGGGGCAAGCG.CCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAG  >  NC_014034/2493961‑2494151

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 23 ≤  ATCG  < 32 ≤  ATCG  < 40 ≤  ATCG