Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,678,900 +C 96.2% intergenic (+29/+58) RCAP_rcc02495 → / ← RCAP_rcc02496 GTPase, EngC family/group 1 glycosyl transferase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,678,9001.→C96.2% 8.5 ‑4.1 ‑0.2132intergenic (+29/+58)RCAP_rcc02495/RCAP_rcc02496GTPase, EngC family/group 1 glycosyl transferase
Reads supporting (aligned to +/- strand):  new base (73/54):  ref base (0/5):  total (73/59)
Fisher's exact test strand distribution p-value = 1.62e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.80e-01

Only 100 of 158 total aligned reads displayed.

GCCCGCGACCGCGCCTTCGGCAAGATGACCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCA.T.CG..CCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACGTGCCGCCCGCCGTCTCGACAAAGCGGA  >  NC_014034/2678800‑2678995
                                                                                                       |                                                                                                
gCCCGCGACCGCGCCTTCGGCAAGATGACCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCA.T.Cg                                                                                                   <  1:465016/101‑1 (MQ=255)
   cgcgACCGCGCCTTCGGCAAGATGACCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCA.T.CG..ccc                                                                                              >  1:424391/1‑101 (MQ=255)
     cgACCGCGCCTTCGGCAAGATGACCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCA.T.CG..ccccc                                                                                            <  2:2428091/101‑2 (MQ=255)
      gACCGCGCCTTCGGCAAGATGACCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCA.T.CG..ccccca                                                                                           >  2:3225009/1‑99 (MQ=255)
      gACCGCGCCTTCGGCAAGATGACCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCA.T.CG..ccccca                                                                                           >  1:3664379/1‑99 (MQ=255)
      gACCGCGCCTTCGGCAAGATGACCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCA.T.CG..ccccca                                                                                           <  1:274326/101‑3 (MQ=255)
       aCCGCGCCTTCGGCAAGATGACCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCA.T.CG..cccccac                                                                                          <  1:1052150/101‑4 (MQ=255)
        ccGCGCCTTCGGCAAGATGACCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCA.T.CG..cccccacc                                                                                         <  1:4315609/101‑5 (MQ=255)
        ccGCGCCTTCGGCAAGATGACCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCA.T.CG..cccccacc                                                                                         <  1:2617012/101‑5 (MQ=255)
                  ggCAAGATGACCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGGA.T.CGC.CCCCACCGCCACCGAcg                                                                                >  2:3956331/1‑101 (MQ=255)
                  ggCAAGATGACCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCA.T.CGC.CCCCACCGCGCCCGAcg                                                                                <  1:539673/101‑1 (MQ=255)
                  ggCAAGATGACCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCA.T.CGC.CCCCACCGCGCCCGAcc                                                                                >  1:3923097/1‑100 (MQ=255)
                     aaGATGACCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCA.T.CGC.CCCCACCGCGCCCGCcgcgc                                                                             >  1:3347320/1‑101 (MQ=255)
                       gATGACCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCA.T.CGC.CCCCACCGCGCCCGACGCgcgg                                                                           >  2:2874426/1‑101 (MQ=255)
                       gATGACCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCA.T.CGC.CCCCACCGCGCCCGACGCgcgg                                                                           >  1:546843/1‑101 (MQ=255)
                        aTGACCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCA.T.CGC.CCCCACCGCGCCCGACGCgcggc                                                                          >  1:1042306/1‑101 (MQ=255)
                        aTGACCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCA.T.CGC.CCCCACCGCGCCCGACGCgcggc                                                                          <  2:4436078/101‑1 (MQ=255)
                         tggcccGGGCGGCGCGGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCA.T.CGC.CCCCACCGCGCCCGACGCgcggcg                                                                         <  2:1337406/98‑1 (MQ=255)
                          ggcccGGGCGGCGCTGCGCGACAAACCCCGGCTGCGCGGCGACTGACGCCGGTTGGGGGGCGGGTGGGGGCA.T.CGC.CCCCACCGCGCCCGACGCGcggcgg                                                                        <  1:2652730/99‑1 (MQ=38)
                           aCCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCA.T.CGC.CCCCACCGCGCCCGCCGCGCGGCggg                                                                       >  1:764858/1‑101 (MQ=255)
                           aCCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCA.T.CGC.CCCCACCGCGCCCGACGCGCGGCggg                                                                       >  2:3531072/1‑101 (MQ=255)
                              cGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCA.T.CGC.CCCCACCGCGCCCGACGCGCGGCGGGCtt                                                                    <  2:1801501/101‑1 (MQ=255)
                              cGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCA.T.CGC.CCCCACCGCGCCCGACGCGCGGCGGGCtt                                                                    >  2:2105828/1‑101 (MQ=255)
                              cGGGCGGCCCTGCGCAACAAATCCCGGCTGCGCGGCGACTGCCGCCGGTTCGGGGGCCGGTGGGGGCA.T.CGC.CCCCACCGCGCCCGACGCGCGGCGGGCtt                                                                    <  2:3481081/101‑1 (MQ=38)
                                ggcggcGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCA.T.CGC.CCCCACCGCGCCCGACGCGCGGCGGGCTTgc                                                                  >  1:1328064/1‑101 (MQ=255)
                                ggcggcGCGGCGCGGCAAGCCCCGGCGGCGCGGCGACTGGCGCCGGTTGGGGGGCGGGGGGGGGCG.C.CGC.CCCCACCGCGCCCGACGCGCGGCGGGCTTgc                                                                  <  1:4540626/101‑1 (MQ=19)
                                ggcggcCCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCA.T.CGC.CCCCACCGCGCCCGACGCGCGGTGGGCTTgc                                                                  <  1:776672/101‑1 (MQ=39)
                                 ggggcgCTGCGCGACAACCCCCGGCTGCGCGGCGACTGCCGCCGGTTGGGGGGCCGGTGGGGCCA.TCCC..CCCCACCGCGCCCGACGCGCGGCGGGCTTgcc                                                                 <  2:3175380/99‑1 (MQ=255)
                                 gcggcgCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCA.T.CGC.CCCCACCGCGCCCGACGCGCGGCGGGCTTgcc                                                                 >  1:897594/1‑101 (MQ=255)
                                  cggcgCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCA.T.CGC.CCCCACCGCGCCCGACGCGCGGCGGGCTTgccc                                                                <  2:2197724/101‑1 (MQ=255)
                                   ggcgCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCA.T.CGC.CCCCACCGCGCCCGACGCGCGGCGGGCTTgcccg                                                               >  1:497918/1‑101 (MQ=255)
                                    gcgcTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCA.T.CGC.CCCCACCGCGCCCGACGCGCGGCGGGCTTgcccgc                                                              <  1:1523778/101‑1 (MQ=255)
                                     cgcTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCA.T.CGC.CCCCACCGCGCCCGACGCGCGGCGGGCTTgcccgcc                                                             >  1:3944360/1‑101 (MQ=255)
                                      gcTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCA.T.CGC.CCCCACCGCGCCCGACGCGCGGCGGGCTTgcccgccc                                                            <  1:3568384/101‑1 (MQ=255)
                                      gcTGCGCGACAAACCCCGGCGGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCA.T.CGC.CCCCACCGCGCCCGACGCGCGGCGGGCTTgcccgccc                                                            <  1:3611717/101‑1 (MQ=38)
                                       cTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCA.T.CGC.CCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCa                                                           <  2:2958616/101‑1 (MQ=255)
                                       cTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCA.T.CGC.CCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCa                                                           >  1:3679205/1‑101 (MQ=255)
                                       cTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCA.T.CGC.CCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCa                                                           >  1:4295549/1‑101 (MQ=255)
                                        tGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCA.T.CGC.CCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCaa                                                          >  2:3869835/1‑101 (MQ=255)
                                        tGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCA.T.CGC.CCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCaa                                                          <  2:405215/101‑1 (MQ=255)
                                         gcgcGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCA.T.CGC.CCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAag                                                         <  2:4135875/101‑1 (MQ=255)
                                         gccccaCAAACCCCGGCTGCGCGGGGACTGACCCCGGTTGGGGGGCCGGTGGGGGCA.T.CGC.CCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAag                                                         <  2:4371670/96‑1 (MQ=25)
                                          cgcgCCAAACCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCA.T.CGC.CCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAagg                                                        <  2:3664018/101‑1 (MQ=37)
                                          cgcgACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCA.T.CGC.CCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAagg                                                        <  2:1805314/101‑1 (MQ=255)
                                          cgcgACAAACCCCGGCTGCGCGGCGACTGCCGCCGGTTCGGGGGCCGGTGGGGGCA.T.CGC.CCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAagg                                                        <  2:4216619/101‑1 (MQ=37)
                                           gcgACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCA.T.CGC.CCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCCagga                                                       >  1:2556721/1‑101 (MQ=255)
                                            cgACAAATCCCGGCTGGGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCA.T.CGC.CCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAaggag                                                      <  2:2986655/101‑1 (MQ=255)
                                             gACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCA.T.CGC.CCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAaggagg                                                     <  2:2677696/101‑1 (MQ=255)
                                              aCAAATCCCGGCTGCGCGGCGACTGACGCCGGTTGGGGGGCCGGTGGGGGCA.C.CGC.CCCCCCCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAggg                                                    <  1:3823532/101‑1 (MQ=25)
                                              aCAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCA.T.CGC.CCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCCCCCAAGGAggg                                                    >  2:4570839/1‑101 (MQ=255)
                                               cAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCA.T.CGC.CCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGgc                                                   <  2:2005181/101‑1 (MQ=255)
                                                aaaTCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCA.T.CGC.CCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGgcc                                                  >  2:1402729/1‑100 (MQ=255)
                                                   ccccGGCTGCGGGGCGCCGGCCGCGGGTCGGGGGGCGGGGGGGGGCACC.CC..CCCCCCCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGccc                                               <  1:2347625/100‑1 (MQ=9)
                                                    cccGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCA.T.CGC.CCCCACCGCGCCCGACGCGCAGCGGGCTTGCCCGCCCAAGGAGGGCGcccc                                              >  1:40473/1‑101 (MQ=255)
                                                     cggccggggcggcgCCGGCCGCGGGTGGGGGGGCGGGGGGGGCCACC.CC..CCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGccccc                                             <  2:3321208/93‑1 (MQ=9)
                                                     ccGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCA.T.CGC.CCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCCAGGAGGGGGccccc                                             >  2:1176623/1‑101 (MQ=37)
                                                     ccGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCA.C.CGC.CCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGccccc                                             <  2:2556547/101‑1 (MQ=255)
                                                      gggCGGCGGGGCGCCTGCCGCCGGTGGGGGGGCGGGGGGGGGCT.TCCC..CCCCCCCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCg                                            <  2:924602/100‑1 (MQ=12)
                                                      cgtcggcgcGGCGACTGGCGCCTGTTCGGTGGCCGGTGGGGGCA.T.CGC.CCCCGTCGCGCCCGACGCGCGGCGGGCTTTCCCGCCCAAGGAGGGCGCCCCCg                                            <  2:791485/96‑1 (MQ=18)
                                                      cGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCA.T.CGC.CCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGGCCCCg                                            >  2:2411484/1‑101 (MQ=255)
                                                        gCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCA.T.CGC.CCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGa                                          <  2:1220441/101‑1 (MQ=255)
                                                         cTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCA.T.CGC.CCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGAt                                         <  2:4203650/101‑1 (MQ=255)
                                                         cTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCA.T.CGC.CCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGAt                                         <  2:3065649/101‑1 (MQ=255)
                                                          tGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCA.T.CGC.CCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGAtc                                        >  1:683512/1‑101 (MQ=255)
                                                           gcgcGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCA.T.CGC.CCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGAtct                                       >  1:1374016/1‑101 (MQ=255)
                                                            cgcgGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCA.T.CGC.CCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGAtctc                                      <  2:424354/101‑1 (MQ=255)
                                                             gcggcgACTGACGCCGGTTCGGGGGCCGGTGGGGGCA.T.CGC.CCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCa                                     >  1:1774926/1‑101 (MQ=255)
                                                              cggcgACTGACGCCGGTTCGGGGGCCGGTGGGGGCA.T.CGC.CCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAg                                    >  1:3051773/1‑101 (MQ=255)
                                                               cgatcttggcGCCGGTTGGGGGGCCGGTGGGGGCA.T.CGC.CCCCCCCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAgc                                   <  1:2736019/92‑1 (MQ=21)
                                                                gcgACTGACGCCGGTTCGGGGGCCGGTGGGGGCA.T.CGC.CCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAgcg                                  >  1:1790456/1‑101 (MQ=255)
                                                                  gACTGACGCCGGTTCGGGGGCCGGTGGGGGCA.T.CGC.CCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCa                                <  1:668260/101‑1 (MQ=255)
                                                                  gACTGACGCCGGTTCGGGGGCCGGTGGGGGCA.T.CGC.CCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCa                                >  1:3831698/1‑101 (MQ=255)
                                                                  gACTGACGCCGGTTCGGGGGCCGGTGGGGGCA.T.CGC.CCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCa                                >  2:3225352/1‑101 (MQ=255)
                                                                     tgccGCGGGTCCGGGGGCCGGTGGGGGCA.T.CGC.CCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACg                             <  1:4670148/98‑1 (MQ=25)
                                                                     tGACGCCGGTTCGGGGGCCGGTGGGGGCA.T.CGC.CCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACg                             <  1:220878/101‑1 (MQ=255)
                                                                     tGACGCCGGTTCGGGGGCCGGTGGGGGCA.T.CGC.CCCCACCGCGCCCGACGCGCGGCGGGCTTACCCGCCCAAGGAGGGCGCCCCCGGATCTCGGCGCAACg                             >  1:3535534/1‑101 (MQ=25)
                                                                      gACGCCGGTTGGGGGGCCGGGGGGGGCA.T.CGC.CCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACGt                            <  2:1710127/101‑1 (MQ=25)
                                                                       aCGCCGGTTCGGGGGCCGGTGGGGGCA.T.CGC.CCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACGTg                           <  2:708461/101‑1 (MQ=255)
                                                                         gCCGGTTCGGGGGCCGGTGGGGGCA.T.CGC.CCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACGTgcc                         <  2:2109592/101‑1 (MQ=255)
                                                                           cGGTTCGGGGGCCGGTGGGGGCA.T.CGC.CCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACGTgccgc                       <  2:4335596/101‑1 (MQ=255)
                                                                            ggTTCGGGGGCCGGTGGGGGCA.T.CGC.CCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGACCTCAGCGCAACGTgccgcc                      >  2:1361468/1‑101 (MQ=255)
                                                                              ttCGGGGGCCGGTGGGGGCA.T.CGC.CCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACGTGccgcccg                    >  2:3625322/1‑101 (MQ=255)
                                                                                cGGGGGCCGGTGGGGGCA.T.CGC.CCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACGTGCcgcccgcc                  >  1:3280080/1‑101 (MQ=255)
                                                                                cGGGGGCCGGTGGGGGCA.T.CGC.CCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACGTGCcgcccgcc                  >  2:1914662/1‑101 (MQ=255)
                                                                                 gggggCGGGTGGGGCCA.T.CGC.CCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCGCCGGATCTCAGCGCAACGTGCCGCccgccg                 <  2:1470948/101‑1 (MQ=25)
                                                                                 gggggCCGGGGGGGGCC.C.CCCCCCCCCCCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACGTGCcgcccgcc                  <  1:4051296/101‑1 (MQ=21)
                                                                                  ggggCCGGTGGGGGCA.T.CGC.CCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACGTGCCGCCCGCCGt                >  1:1675241/1‑101 (MQ=255)
                                                                                    ggCCGGTGGGGGCA.T.CGC.CCCCGCCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACGTGCCGCCCGCCGtct              <  2:665835/101‑1 (MQ=255)
                                                                                     gCCGGTGGGGCCA.TCCC..CCCCACCGCGCCCGCCGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACGTGCCGCCCGCCGtctc             <  1:3422072/101‑1 (MQ=37)
                                                                                       cGGTGGGGGCA.T.CGC.CCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACGTGCCGCCCGCCGTCTCGa           <  2:497870/101‑1 (MQ=255)
                                                                                        ggTGGGGGCA.T.CGC.CCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGGGCCCCCGGATCTCAGCGCAACGTGCCGCCCGCCGTCTCGAc          >  2:570255/1‑101 (MQ=255)
                                                                                        ggTGGGGGCA.T.CGC.CCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACGTGCCGCCCGCCGTCTCGAc          >  2:3605429/1‑101 (MQ=255)
                                                                                        ggTGGGGGCA.T.CGC.CCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACGTGCCGCCCGCCGTCTCGAc          >  1:4604856/1‑101 (MQ=255)
                                                                                        ggTGGGGGCA.T.CGC.CCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACGTGCCGCCCGCCGTCTCGAc          >  2:4322892/1‑101 (MQ=255)
                                                                                          tGGGGGCA.T.CGC.CCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACGTGCCGCCCGCCGTCTCGACaa        >  1:951149/1‑101 (MQ=255)
                                                                                            ggggCA.T.CGC.CCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACGTGCCGCCCGCCGTCTCGACAAAg      >  1:1254030/1‑101 (MQ=255)
                                                                                             gggCA.T.CGC.CCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACGTGCCGCCCGCCGTCTCGACAAAGc     >  1:827090/1‑101 (MQ=255)
                                                                                               gCA.T.CGC.CCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACGTGCCGCCCGCCGTCTCGACAAAGCgg   >  1:78043/1‑101 (MQ=255)
                                                                                                cA.T.CGC.CCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACGTGCCGCCCGCCGTCTCGACAAAGCGGa  >  2:626539/1‑101 (MQ=255)
                                                                                                cA.T.CGC.CCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACGTGCCGCCCGCCGTCTCGACAAAGCGGa  >  1:2948987/1‑101 (MQ=255)
                                                                                                       |                                                                                                
GCCCGCGACCGCGCCTTCGGCAAGATGACCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCA.T.CG..CCCCACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACGTGCCGCCCGCCGTCTCGACAAAGCGGA  >  NC_014034/2678800‑2678995

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 22 ≤  ATCG  < 31 ≤  ATCG  < 39 ≤  ATCG