Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,747,869	+G	100%	intergenic (‑201/+92)	RCAP_rcc02553 ← / ← RCAP_rcc02554	hypothetical protein/NmrA family protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,747,869	1	.→G	100.0%	54.1	21	intergenic (‑201/+92)	RCAP_rcc02553/RCAP_rcc02554	hypothetical protein/NmrA family protein
Reads supporting (aligned to +/- strand):  new base (19/2):  ref base (0/0):  total (19/2)

CGACCCGCGTTTCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.C.GCGGCCGAGAAGCGCCCGCC.GGCCGGCTGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAGGTCAGCTC  >  NC_014034/2747770‑2747962                                                                                                       |                                                                                              ccccccGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.CGGCGGGCGAGAAGcgcccgcc                                                                                                <  2:3400290/98‑1 (MQ=25)   ccccGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.CGGCGGGCGAGAAGCGCCCgcc.gg                                                                                             <  1:2099466/100‑1 (MQ=25)     ccGCGTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.CGGCGGGCGAGAAGCGCCCgcc.gggc                                                                                           <  2:19395/101‑3 (MQ=25)      cgcgTGCCCCCCCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.CGGCGGGCGAGAAGCGCCCgcc.gggcc                                                                                          <  1:3348533/101‑4 (MQ=21)      cgcgTCCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.CGGCGGGCGAGAAGCGCCCgcc.gggcc                                                                                          <  1:2743619/101‑4 (MQ=21)      ccttctcccccGCCCGCAGCGCCCGGTTGCGGCATGCCCGCTTCAGCCCGCACCACAGAGGGCGGCGCCCGGCC.CGGCGGGCGAGAAGCGCCCgcc.gggcc                                                                                          <  1:2618177/94‑4 (MQ=21)        ggcccccccGCCCGCAGCGCCCGGTTGCGGCATGGCCCCTTCAGCCCGCACCACAGAGGGCAGCCCCCGGCC.CGGCGGGCGAGAAGCGCCCgcc.gggccgg                                                                                        <  1:3540001/96‑6 (MQ=21)                 gcccgcAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.CGGCGGGCGAGCAGCGCCCGCCGGGCCGGGCGCTGccc                                                                                >  1:2730801/1‑101 (MQ=21)                     gcAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.CGGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGCgg                                                                            >  2:17465/1‑101 (MQ=21)                     cccgcgcCCGGTGCCGGCATGGCGGCTTCAGCCCGCACCACAGAGGGCAGCGCTGGGCCGG.GCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAgg                                                                            <  2:201514/98‑1 (MQ=12)                                                                           cggcc.cgGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGCCACAGGACCTCTGTGACGCATGGcagaacggaag                      >  1:748448/1‑91 (MQ=17)                                                                            ggcc.cgGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCCTGGTCAAGGCGAtcg                     >  2:1929585/1‑99 (MQ=18)                                                                            ggcc.cgGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATgg                     >  1:2973239/1‑101 (MQ=18)                                                                              gcccggcgGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGc                    >  1:2874694/6‑101 (MQ=18)                                                                              gcccggcgGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGc                    >  2:3444205/6‑101 (MQ=18)                                                                                ggggggggcGAGACGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCt                  <  2:2730603/93‑1 (MQ=21)                                                                                ccggcgGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCt                  >  2:1544804/4‑101 (MQ=21)                                                                                 cggcgGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCtt                 >  1:4234037/3‑101 (MQ=21)                                                                                  ggcgGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTg                >  2:789648/2‑101 (MQ=21)                                                                                  ggcgGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTg                >  1:254778/2‑101 (MQ=21)                                                                                    cgGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGc              >  1:3710024/1‑101 (MQ=21)                                                                                     gggcGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCCTGGCCAAGGCGATGGCCTTGGcc             >  2:4117680/4‑101 (MQ=18)                                                                                        cGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTTGGCCCTCGGGGGGCACAGGACCTCTATGACCCATGGGCAAGGCGGTGGCCTCGGCCCAg          >  1:4029713/1‑101 (MQ=18)                                                                                         gagaAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAgg         >  1:2615270/1‑101 (MQ=21)                                                                                         gagaAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAgg         >  1:193052/1‑101 (MQ=21)                                                                                          agaAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAGGt        >  1:3509186/1‑101 (MQ=21)                                                                                          agaAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAGGt        >  2:3340861/1‑101 (MQ=21)                                                                                          agaAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGAATGGCCAAGGCGATGGCCTTGGCCAAGGt        >  2:3373281/1‑101 (MQ=21)                                                                                            aaGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAGGTCa      >  1:842795/1‑101 (MQ=21)                                                                                                gcccgccgGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAGGTCAGCTc  <  2:254754/101‑1 (MQ=21)                                                                                                       |                                                                                              CGACCCGCGTTTCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.C.GCGGCCGAGAAGCGCCCGCC.GGCCGGCTGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAGGTCAGCTC  >  NC_014034/2747770‑2747962 Base quality scores:  ATCG  < 3 ≤  ATCG  < 16 ≤  ATCG  < 22 ≤  ATCG  < 31 ≤  ATCG  < 39 ≤  ATCG