BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	279,645	+A	2.3%	intergenic (‑47/‑33)	RCAP_rcc00242 ← / → dapD	hypothetical protein/2,3,4,5‑tetrahydropyridine‑2,6‑dicarboxylate N‑succinyltransferase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	279,645	0	.→A	2.3%	16.6 0.0 ‑0.2	261	intergenic (‑47/‑33)	RCAP_rcc00242/dapD	hypothetical protein/2,3,4,5‑tetrahydropyridine‑2,6‑dicarboxylate N‑succinyltransferase
Reads supporting (aligned to +/- strand): new base (3/3): ref base (138/117): total (141/120)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.94e-01

Only 100 of 282 total aligned reads displayed.


TTTTGCATCCATGATGCTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAACCCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGTTCGCGACACCCTCACGCC  >  NC_014034/279545‑279742


ttttGCATCCATGATGCTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAAc                                                                                                   >  2:4404864/1‑101 (MQ=255)


 tttGCATCCATGATGCTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAAcc                                                                                                  >  2:1682681/1‑101 (MQ=255)


  ttGCATCCATGATGCTGTCACGGAACGGGTGGGTACGCCTTTCGTCTTTCATATCCTTCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAAcca                                                                                                 <  1:3992160/101‑2 (MQ=255)


  ttGCATCCATGATGCTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAAcca                                                                                                 <  2:4560099/101‑2 (MQ=255)


   tGCATCCATGATGCTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAAccat                                                                                                >  1:4075024/1‑99 (MQ=255)


   tGCATCCATGATGCTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTACTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAAccat                                                                                                <  2:4628446/101‑3 (MQ=255)


    gCATCCATGATGCTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAAccatg                                                                                               <  2:1354066/101‑4 (MQ=255)


       tCCATGATGCTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAAt                                                                                           <  1:2926485/101‑1 (MQ=255)


        ccATGATGCTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATg                                                                                          <  2:2750128/101‑1 (MQ=255)


         cATGATGCTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGc                                                                                         >  1:2826585/1‑101 (MQ=255)


            gatgCTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCAc                                                                                      >  1:3526346/1‑101 (MQ=255)


            gatgCTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCAc                                                                                      >  1:4234569/1‑101 (MQ=255)


             atgCTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACt                                                                                     <  2:2075762/101‑1 (MQ=255)


             atgCTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACt                                                                                     <  2:2452006/101‑1 (MQ=255)


               gCTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTg                                                                                   <  2:2567863/101‑1 (MQ=255)


                 tGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGaa                                                                                 <  2:2140561/101‑1 (MQ=255)


                 tGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGaa                                                                                 <  1:3230788/101‑1 (MQ=255)


                 tGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTAaa                                                                                 <  2:1568807/101‑1 (MQ=255)


                  gTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGaaa                                                                                <  1:3866278/101‑1 (MQ=255)


                  gTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGaaa                                                                                <  2:2377785/101‑1 (MQ=255)


                    cACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAaga                                                                              <  2:879031/101‑1 (MQ=255)


                     aCGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAagag                                                                             >  2:1073654/1‑101 (MQ=255)


                     aCGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAagag                                                                             >  1:2212831/1‑101 (MQ=255)


                     aCGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAagag                                                                             >  1:3393688/1‑101 (MQ=255)


                     aCGGAACGGGGGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAagag                                                                             <  2:933571/101‑1 (MQ=255)


                       ggAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCCTTACCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGa                                                                           >  2:3879400/1‑101 (MQ=255)


                        gAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGAc                                                                          <  2:3009441/101‑1 (MQ=255)


                         aaCGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTGGCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGAcc                                                                         <  2:4033250/101‑1 (MQ=255)


                           cGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGt                                                                       <  1:1172876/101‑1 (MQ=255)


                            gggTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGtt                                                                      >  2:2984511/1‑101 (MQ=255)


                            gggTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGtt                                                                      <  1:2810118/101‑1 (MQ=255)


                            gggTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGtt                                                                      >  1:1612259/1‑101 (MQ=255)


                               tGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCg                                                                   >  1:2314000/1‑101 (MQ=255)


                               tGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCg                                                                   <  2:1482376/101‑1 (MQ=255)


                                      ggCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGa                                                            >  1:2888170/1‑101 (MQ=255)


                                      ggCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGa                                                            <  1:748816/101‑1 (MQ=255)


                                       gCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGaa                                                           <  2:2591323/101‑1 (MQ=255)


                                         tttCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACg                                                         <  1:91416/101‑1 (MQ=255)


                                         tttCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACg                                                         >  2:1019363/1‑101 (MQ=255)


                                           tCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGcc                                                       <  2:2841677/101‑1 (MQ=255)


                                             gTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCAt                                                     >  1:958651/1‑101 (MQ=255)


                                               cTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGc                                                   >  1:1196230/1‑101 (MQ=255)


                                                 ttCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCagatcg                                                 >  1:2122434/1‑96 (MQ=255)


                                                 ttCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTc                                                 <  1:3756770/101‑1 (MQ=255)


                                                  tCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCg                                                >  1:4492523/1‑101 (MQ=255)


                                                    atatCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGaa                                              <  1:831290/101‑1 (MQ=255)


                                                     tatCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAg                                             <  1:1432376/101‑1 (MQ=255)


                                                     tatCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGAGGTCGAAGGCCACACTCGagg                                             >  2:1251715/1‑99 (MQ=255)


                                                     tatCCTGCTAATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAg                                             >  2:664626/1‑101 (MQ=255)


                                                       taattcTCATTGCGCGAGGGGATTACACCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACCCCATGCGCGAAGCt                                           <  2:1839055/96‑1 (MQ=255)


                                                        ccTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTg                                          <  1:733201/101‑1 (MQ=255)


                                                          tGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGcc                                        >  1:4380345/1‑101 (MQ=255)


                                                           gCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCa                                       >  2:1165444/1‑101 (MQ=255)


                                                            cTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCAt                                      >  2:3952025/1‑101 (MQ=255)


                                                             tCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATc                                     <  1:2573741/101‑1 (MQ=255)


                                                             tCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATc                                     >  2:2340409/1‑101 (MQ=255)


                                                              cATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCg                                    >  1:1273203/1‑101 (MQ=255)


                                                              cATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTGGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCg                                    <  1:2196094/101‑1 (MQ=255)


                                                               aTTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGa                                   >  2:227391/1‑101 (MQ=255)


                                                                ttGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGaa                                  <  2:323374/101‑1 (MQ=255)


                                                                 tctgcgAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAg                                 <  2:3589928/98‑1 (MQ=255)


                                                                 tGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGACGCTGCCATCGAAg                                 >  1:2383115/1‑101 (MQ=255)


                                                                 tGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAg                                 <  2:3393435/101‑1 (MQ=255)


                                                                 tGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAg                                 <  1:795616/101‑1 (MQ=255)


                                                                 tGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAg                                 >  1:3904716/1‑101 (MQ=255)


                                                                  gcgcGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAgc                                >  1:4406072/1‑101 (MQ=255)


                                                                  gcgcGAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAgc                                <  1:3201749/101‑1 (MQ=255)


                                                                     cgAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAgccgc                             >  2:3838538/1‑101 (MQ=255)


                                                                      gAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAgccgcc                            >  2:22635/1‑101 (MQ=255)


                                                                      gAGGGGCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAgccgcc                            >  2:417720/1‑101 (MQ=255)


                                                                        ggggCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTg                          <  1:2265309/101‑1 (MQ=255)


                                                                        ggggCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTg                          >  2:224012/1‑101 (MQ=255)


                                                                          ggCTTTCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTggg                        <  2:4234281/101‑1 (MQ=255)


                                                                             tttCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAgg                     <  1:1056531/101‑1 (MQ=255)


                                                                             tttCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAgg                     <  1:2984740/101‑1 (MQ=255)


                                                                             tttCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAgg                     >  1:3093760/1‑101 (MQ=255)


                                                                              ttCCCCAACTATATGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGt                    <  2:1422048/101‑1 (MQ=255)


                                                                              ttCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAggg                    >  1:509682/1‑100 (MQ=255)


                                                                              ttCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGt                    >  2:1531686/1‑101 (MQ=255)


                                                                               tCCCCAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGtt                   >  1:4586015/1‑101 (MQ=255)


                                                                                tcccAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGTTc                  <  2:604572/100‑1 (MQ=255)


                                                                                ccccAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGTTc                  <  1:167284/101‑1 (MQ=255)


                                                                                  ccAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGTTcgc                <  1:1019446/101‑1 (MQ=255)


                                                                                   cAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGTTcgcg               <  2:983727/101‑1 (MQ=255)


                                                                                   cAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGTTcgcg               >  2:3770177/1‑101 (MQ=255)


                                                                                   cAACTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGTTcgcg               >  1:251229/1‑101 (MQ=255)


                                                                                    aaCTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGTTCGCGa              <  1:1514009/101‑1 (MQ=255)


                                                                                      cTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGTTCGCGaca            >  1:2057988/1‑101 (MQ=255)


                                                                                      cTATAGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGTTCGCGaca            <  2:4364475/101‑1 (MQ=255)


                                                                                        ataGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGTTCGCGACAcc          >  1:2673320/1‑101 (MQ=255)


                                                                                        ataGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGTTCGCGACAcc          >  2:1881586/1‑101 (MQ=255)


                                                                                         taGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGTTCGCGACAccc         <  2:2821012/101‑1 (MQ=255)


                                                                                         taGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCCAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGTTCGCGACAccc         >  1:7262/1‑101 (MQ=255)


                                                                                          aGGGGGAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGTTCGCGACACCCt        <  1:57324/101‑1 (MQ=255)


                                                                                           gggggAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGTTCGCGACACCCTc       <  1:1864120/101‑1 (MQ=255)


                                                                                             gggAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGTTCGCGACACCCTCAc     <  1:2619697/101‑1 (MQ=255)


                                                                                             gggAAAA.CCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGTTCGCGACACCCTCAc     <  2:737618/101‑1 (MQ=255)


                                                                                               ggaaaACCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGTTCGCGACACCCTCACg    <  2:1960651/99‑1 (MQ=255)


                                                                                                gaaaACCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGTTCGCGACACCCTCACGc   <  2:3124355/100‑1 (MQ=255)


                                                                                                gaaaACCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGGTCGCGACACCCTCACGc   >  1:4317129/2‑101 (MQ=255)


TTTTGCATCCATGATGCTGTCACGGAACGGGTGGGAACGGCTTTCGTCTTTCATATCCTGCTCATTGCGCGAGGGGCTTTCCCCAACTATAGGGGGAAAACCCATGAATGCCACTTGAAAGAGGACCGTTCCGATGTCGAACGCCATGCTCGAAGCTGCCATCGAAGCCGCCTGGGAGGTTCGCGACACCCTCACGCC  >  NC_014034/279545‑279742

Base quality scores:


ATCG

< 3 ≤


ATCG

< 24 ≤


ATCG

< 31 ≤


ATCG

< 35 ≤


ATCG

< 41 ≤


ATCG