Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,808,557 +G 100% intergenic (+112/+42) pyrD2 → / ← acsA2 dihydroorotate oxidase/acetate‑‑CoA ligase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,808,5571.→G100.0% 125.1 63intergenic (+112/+42)pyrD2/acsA2dihydroorotate oxidase/acetate‑‑CoA ligase
Reads supporting (aligned to +/- strand):  new base (45/18):  ref base (0/0):  total (45/18)

CTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGA.GA.GTGGCGCAGGCAAGTCGGGGGGCGGCGCCCCCCGCC  >  NC_014034/2808457‑2808593
                                                                                                      |                                    
cTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGga.ga                                       <  1:931460/101‑1 (MQ=38)
cTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGga.ga                                       <  2:3566915/101‑1 (MQ=38)
 tGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGa.ga.g                                     <  2:4283829/101‑1 (MQ=37)
  gCGGTGGTCTCTCTCCCCCTCCTCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCGCCCTGGCGCAAATCTCCCGGGGGGa.ga.gg                                    <  2:1616657/101‑2 (MQ=11)
   cGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGa.ga.ggt                                   >  1:1028741/1‑99 (MQ=18)
   cGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGa.ga.ggt                                   >  2:3435284/1‑99 (MQ=18)
   cGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGa.ga.ggg                                   >  2:3531627/1‑99 (MQ=21)
   cGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCACCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGa.ga.ggt                                   <  1:4544326/101‑3 (MQ=18)
    gggggTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGa.ga.ggtg                                  <  1:4110740/101‑4 (MQ=17)
    gggggTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGa.ga.ggtg                                  >  2:1284413/1‑98 (MQ=17)
    gggggTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGa.ga.ggtg                                  >  2:1524379/1‑98 (MQ=17)
    gggggTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGa.ga.ggtg                                  >  2:1528740/1‑98 (MQ=17)
    gggggTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGa.ga.ggtg                                  <  2:1762889/101‑4 (MQ=17)
    gggggTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGa.ga.ggtg                                  <  2:2132475/101‑4 (MQ=17)
    gggggTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGa.ga.ggtg                                  >  1:262033/1‑98 (MQ=17)
     ggggTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGa.ga.ggtgg                                 <  2:254350/101‑5 (MQ=14)
     ggggTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGa.ga.ggtgg                                 >  1:1793671/1‑97 (MQ=17)
      gggTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGa.ga.ggtggc                                <  1:1803324/101‑6 (MQ=11)
      gggTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGa.ga.ggtggc                                <  1:3348439/101‑6 (MQ=11)
         tttCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGA.GAGGTGGCGCa                              >  1:4484631/1‑101 (MQ=9)
          ttCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGA.GAGGTGGCGCAg                             >  1:2688415/1‑101 (MQ=12)
          ttCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGA.GAGGTGGCGCAg                             >  1:3434618/1‑101 (MQ=12)
          ttCAAACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGC.GAGGTGGCGCAg                             >  2:704637/1‑101 (MQ=11)
           tCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGA.GAGGTGGCGCAgg                            <  1:2964797/101‑1 (MQ=12)
            cacaCCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGA.GAGGTGGCGCAGGc                           >  1:2225684/1‑101 (MQ=16)
            cacaCCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGA.GAGGTGGCGCAGGc                           <  1:4020856/101‑1 (MQ=12)
            cacaCCCCCGCACCCCCGTGGCGTATTTGCGACAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGA.GAGGTGGCGCAGGc                           >  1:4143631/1‑101 (MQ=255)
             acacCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGA.GAGGTGGCGCAGGCa                          >  2:3652530/1‑101 (MQ=12)
             acacCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGA.GAGGTGGCGCAGGCa                          >  2:355814/1‑101 (MQ=12)
             acacCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGA.GAGGGGGCGCAGGCa                          >  1:4036682/1‑101 (MQ=255)
              cccccccgcacccccgTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGA.GAGGTGGCGCAGGCaa                         <  2:2385668/99‑1 (MQ=12)
              cacccccgcacccccgTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGA.GAGGTGGCGCAGGCaa                         <  1:4459829/101‑1 (MQ=12)
               acccccgcacccccgTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGA.GAGGTGGCGCAGGCAAg                        <  1:478016/101‑1 (MQ=12)
               acccccgcacccccgTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGA.GAGGTGGCGCAGGCAAg                        >  1:68377/1‑101 (MQ=12)
               acccccgcacccccgTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGA.GAGGTGGCGCAGGCAAg                        >  2:4501774/1‑101 (MQ=19)
               acccccgcacccccgTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGA.GAGGTGGCGCAGGCAAg                        >  1:4378051/1‑101 (MQ=19)
                cccccgcacccccgTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGA.GAGGTGGCGCAGGCAAGt                       <  1:4669577/101‑1 (MQ=12)
                 ccccgcacccccgTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGA.GAGGTGGCGCAGGCAAGTc                      <  2:2671213/101‑1 (MQ=12)
                   ccgcacccccgTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGA.GAGGTGGCGCAGGCAAGTCgg                    <  1:2581326/101‑1 (MQ=12)
                   ccgcacccccgTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGA.GAGGTGGCGCAGGCAAGTCgg                    <  1:467541/101‑1 (MQ=12)
                    cgcacccccgTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGA.GAGGTGGCGCAGGCAAGTCggg                   >  1:2038381/1‑101 (MQ=19)
                      cacccccgTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGA.GAGGTGGCGCAGGCAAGTCggggg                 >  2:4504001/1‑101 (MQ=19)
                      cacccccgTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGA.GAGGGGGCGCAGGCAAGTCggggg                 >  2:1165587/1‑101 (MQ=255)
                       acccccgTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGA.GAGGTGGCGCAGGCAAGTCgggggg                >  2:2728158/1‑101 (MQ=12)
                       acccccgTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGA.GAGGTGGCGCAGGCAAGTCgggggg                <  1:2913753/101‑1 (MQ=16)
                       acccccgTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGA.GAGGTGGCGCAGGCAAGTCgggggg                <  1:293028/101‑1 (MQ=12)
                        cccccgTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGA.GAGGTGGCGCAGGCAAGTCGGGGggc               <  2:1257960/101‑1 (MQ=12)
                          cccgTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGA.GAGGTGGCGCAGGCAAGTCGGGGggcgg             >  2:2345943/1‑101 (MQ=16)
                          cccgTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGA.GAGGTGGCGCAGGCAAGTCGGGGggcgg             >  1:2931465/1‑101 (MQ=16)
                           ccgTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGGG.GGGGCGCAGGCAAGTCGGGGggcggc            >  1:679994/1‑101 (MQ=11)
                           ccgTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGA.GAGGTGGCGCAGGCAAGTCGGGGggcggc            <  2:2877303/101‑1 (MQ=16)
                           ccgTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGA.GAGGTGGCGCAGGCAAGTCGGGGggcggc            >  1:2930201/1‑101 (MQ=16)
                             gTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCTTTGCGCAAATCTCCCGGGGGGT.GAGGTGGCGCAGGCCAGTCGGGGGGCGgcgc          >  1:770589/1‑101 (MQ=11)
                             gTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGA.GAGGTGGCGCAGGCAAGTCGGGGGGCGgcgc          >  2:4380031/1‑101 (MQ=12)
                             gTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGA.GAGGTGGCGCAGGCAAGTCGGGGGGCGgcgc          >  2:834904/1‑101 (MQ=16)
                             gTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGA.GAGGTGGCGCAGGCAAGTCGGGGGGCGgcgc          <  1:4572647/101‑1 (MQ=12)
                              tGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGA.GAGGTGGCGCAGGCAAGTCGGGGGGCGGCGcc         <  1:3417196/101‑1 (MQ=16)
                              tGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGA.GAGGTGGCGCAGGCAAGTCGGGGGGCGGCGcc         >  1:3751261/1‑101 (MQ=16)
                              tGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGA.GAGGTGGCGCAGGCAAGTCGGGGGGCGGCGcc         >  1:1032103/1‑101 (MQ=16)
                              tGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGA.GAGGTGGCGCAGGCAAGTCGGGGGGCGGCGcc         >  1:3877466/1‑101 (MQ=12)
                               ggCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGA.GAGGTGGCGCAGGCAAGTCGGGGGGCGGCGccc        >  2:1645637/1‑101 (MQ=16)
                               ggCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGA.GAGGTGGCGCAGGCAAGTCGGGGGGCGGCGccc        >  1:3739483/1‑101 (MQ=16)
                               ggCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGA.GAGGTGGCGCAGGCAAGTCGGGGGGCGGCGccc        >  1:1416400/1‑101 (MQ=16)
                               ggCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGA.GAGGTGGCGCAGGCAAGTCGGGGGGCGGCGccc        >  1:2448104/1‑101 (MQ=16)
                                gCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGA.GAGGTGGCGCAGGCAAGTCGGGGGGCGGCGcccc       <  1:1106524/101‑1 (MQ=12)
                                 cGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGA.GAGGTGGCGCAGGCAAGTCGGGGGGCGGCGccccc      >  2:3603382/1‑101 (MQ=16)
                                 cGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGA.GAGGTGGCGCAGGCAAGTCGGGGGGCGGCGccccc      <  1:1524488/101‑1 (MQ=12)
                                 cGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGA.GAGGTGGCGCAGGCAAGTCGGGGGGCGGCGccccc      >  2:518977/1‑101 (MQ=16)
                                 cGTATGTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGTGA.GAGGTGGCGCAGGCAAGTCGGGGGGCGGCGccccc      <  2:2517047/101‑1 (MQ=11)
                                  gTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGA.GAGGTGGCGCAGGCAAGTCGGGGGGCGGCGcccccc     >  2:3139021/1‑101 (MQ=12)
                                  gTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGA.GAGGTGGCGCAGGCAAGTCGGGGGGCGGCGcccccc     >  2:2846307/1‑101 (MQ=12)
                                  gTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGA.GAGGTGGCGCAGGCAAGTCGGGGGGCGGCGcccccc     >  1:4299327/1‑101 (MQ=12)
                                  gTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGA.GAGGTGGCGCAGGCAAGTCGGGGGGCGGCGcccccc     >  2:1992772/1‑101 (MQ=12)
                                   tATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGA.GAGGTGGCGCAGGCAAGTCGGGGGGCGGCGCCCCCCg    >  1:1535715/1‑101 (MQ=12)
                                   tATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGA.GAGGTGGCGCAGGCAAGTCGGGGGGCGGCGCCCCCCg    >  2:3675410/1‑101 (MQ=12)
                                   tATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGA.GAGGTGGCGCAGGCAAGTCGGGGGGCGGCGCCCCCCg    >  2:1910726/1‑101 (MQ=12)
                                   tATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGA.GAGGTGGCGCAGGCAAGTCGGGGGGCGGCGCCCCCCg    >  1:1035198/1‑101 (MQ=12)
                                    aTTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGA.GAGGTGGCGCAGGCAAGTCGGGGGGCGGCGCCCCCCGc   >  1:4232172/1‑101 (MQ=9)
                                    aTTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGA.GAGGTGGCGCAGGCAAGTCGGGGGGCGGCGCCCCCCGc   >  1:1062730/1‑101 (MQ=9)
                                    aTTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGA.GAGGTGGCGCAGGCAAGTCGGGGGGCGGCGCCCCCCGc   >  1:887796/1‑101 (MQ=9)
                                    aTTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGA.GAGGTGGCGCAGGCAAGTCGGGGGGAGGAGACCCCCGc   >  2:3811221/1‑101 (MQ=11)
                                    aTTGGCGTCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGA.GAGGTGGCGCAGGCAAGTCGGGGGGCGGCGcccccccc   >  2:955793/1‑99 (MQ=11)
                                     tttGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGA.GAGGTGGCGCAGGCAAGTCGGGGGGCGGCGCCCCCCGcc  >  2:637036/1‑101 (MQ=2)
                                     tttGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGA.GAGGTGGCGCAGGCAAGTCGGGGGGCGGCGCCCCCCGcc  >  2:2567442/1‑101 (MQ=2)
                                     tttGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGA.GAGGTGGCGCAGGCAAGTCGGGGGGCGGCGCCCCCCGcc  >  2:1377949/1‑101 (MQ=2)
                                     tttGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGA.GAGGTGGCGCAGGCAAGTCGGGGGGCGGCGCCCCCCGcc  >  1:3886935/1‑101 (MQ=2)
                                                                                                      |                                    
CTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGA.GA.GTGGCGCAGGCAAGTCGGGGGGCGGCGCCCCCCGCC  >  NC_014034/2808457‑2808593

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG