Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,953,316 +T 1.6% intergenic (+205/+173) RCAP_rcc02764 → / ← ilvE1 hypothetical protein/branched‑chain‑amino‑acid transaminase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,953,3161.→T 1.6% 4.5 ‑1.3 ‑0.0182intergenic (+205/+173)RCAP_rcc02764/ilvE1hypothetical protein/branched‑chain‑amino‑acid transaminase
Reads supporting (aligned to +/- strand):  new base (3/0):  ref base (102/77):  total (105/77)
Fisher's exact test strand distribution p-value = 2.64e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.93e-01

Only 100 of 219 total aligned reads displayed.

CCCGCCTTGGTCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCC..CGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCTCGCCCGCCGGGGCAGGCGCCGGCCTCTGTTGCGCGCGGGGCAGACGGTCTT  >  NC_014034/2953218‑2953416
                                                                                                                                                                                                         
 ccGCCTTGGTCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGAAATCTGCGGTCACCACCGCCGGGGa                                                                                                     >  1:3872323/1‑101 (MQ=255)
   gCCTTGGTCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGAcc                                                                                                   <  1:1627490/101‑1 (MQ=255)
      ttGGTCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCaaa                                                                                                >  1:278019/1‑101 (MQ=255)
      ttGGTCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCaaa                                                                                                <  1:3200353/101‑1 (MQ=255)
      ttGGTCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCAACGCCGGGGACCaaa                                                                                                <  1:927482/101‑1 (MQ=255)
       tGGTCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAg                                                                                               <  1:1693358/101‑1 (MQ=255)
        ggTCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGt                                                                                              >  1:668039/1‑101 (MQ=255)
         gTCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTc                                                                                             >  1:801599/1‑101 (MQ=255)
         gTCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTc                                                                                             <  2:4369232/101‑1 (MQ=255)
            ttttCGGTGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGCGACGCGGCGATGCGAGACGTCTGCAGTCACCACCGNCGGGGACCAACGtaggc                                                                                          >  2:1411821/1‑97 (MQ=255)
             tttCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCC..Cg                                                                                       <  1:2416757/101‑1 (MQ=255)
              ttCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCC..Cgg                                                                                      <  1:4350591/101‑1 (MQ=255)
              ttCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCC..Cgg                                                                                      >  1:3753889/1‑101 (MQ=255)
               tCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCC..CGGc                                                                                     >  2:3697/1‑101 (MQ=255)
                 gggggCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCC..CGGCCa                                                                                   <  1:1407608/101‑1 (MQ=255)
                 gggggCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCC..CGGCCa                                                                                   <  1:3599472/101‑1 (MQ=255)
                   gggCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCC..CGGCCAgc                                                                                 <  2:2538910/101‑1 (MQ=255)
                   gggCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCC..CGGCCAgc                                                                                 >  1:1490887/1‑101 (MQ=255)
                     gcgcATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCC..CGGCCAgcgc                                                                               <  1:1034152/101‑1 (MQ=255)
                      cgcATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCC..CGGCCAGcgcc                                                                              >  2:815262/1‑101 (MQ=255)
                      cgcATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCC..CGGCCAGcgcc                                                                              <  1:1166844/101‑1 (MQ=255)
                      cgcATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCC..CGGCCAGcgcc                                                                              <  1:2908150/101‑1 (MQ=255)
                       gcATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCC..CGGCCAGcgccc                                                                             <  2:2325000/101‑1 (MQ=255)
                       gcATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCC..CGGCCAGcgccc                                                                             >  2:1354830/1‑101 (MQ=255)
                             tctGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCC..CGGCCAGCGcccgccccg                                                                       <  1:2250988/101‑1 (MQ=255)
                             tctGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCC..CGGCCAGCGcccgccccg                                                                       >  1:2592568/1‑101 (MQ=255)
                             tctGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCC..CGGCCAGCGcccgccccg                                                                       >  2:3845895/1‑101 (MQ=255)
                             tctGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCC..CGGCCAGCGcccgccccg                                                                       <  1:2409508/101‑1 (MQ=255)
                                gAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCC..CGGCCAGCGCCcgccccgccc                                                                    >  2:2413567/1‑101 (MQ=255)
                                 gaaGGGCCTTACCTGGGCGCCGCCGGTGGCGCGGGCATGGGAGACATCCGCAGGCCCCCCCGCCGGGGACCAAAGGCCC..CGGCCAGCGCCCGCCCCGCCCa                                                                   <  2:2260111/100‑1 (MQ=16)
                                 aaaGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCC..CGGCCAGCGCCCGCCCCGCCCa                                                                   >  1:70446/1‑101 (MQ=255)
                                 aaaGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCC..CGGCCAGCGCCCGCCCCGCCCa                                                                   >  2:2705879/1‑101 (MQ=255)
                                  aaGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCCCCACCGCCGGGGACCAAAGTCCC..CGGCCAGCGCCCGCCCCGCCCAt                                                                  <  2:4606119/101‑1 (MQ=255)
                                  aaGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCCCCGCCGGGGACCAAAGTCCC..CGGCCAGCGCCCGCCCCGCCCAt                                                                  <  1:4295503/101‑1 (MQ=255)
                                  aaGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCC..CGGCCAGCGCCCGCCCCGCCCAt                                                                  <  1:3962022/101‑1 (MQ=255)
                                  aaGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCC..CGGCCAGCGCCCGCCCCGCCCAt                                                                  <  1:3405820/101‑1 (MQ=255)
                                  aaGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCC..CGGCCAGCGCCCGCCCCGCCCAt                                                                  >  2:359491/1‑101 (MQ=255)
                                  aaGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCC..CGGCCAGCGCCCGCCCCGCCAAt                                                                  <  1:2663052/101‑1 (MQ=255)
                                    gcgcCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCC..CGGCCAGCGCCCGCCCCGCCCATAg                                                                <  1:4229512/101‑1 (MQ=255)
                                      gcCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCC..CGGCCAGCGCCCGCCCCGCCCATAGCg                                                              >  1:2502885/1‑101 (MQ=255)
                                      gcCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCC..CGGCCAGCGCCCGCCCCGCCCATAGCg                                                              >  2:3864672/1‑101 (MQ=255)
                                       ccTGGCTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCC..CGGCCAGCGCCCGCCCCGCCCATAGCgg                                                             <  2:1537764/101‑1 (MQ=255)
                                       ccTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCC..CGGCCAGCGCCCGCCCCGCCCATAGCgg                                                             <  1:1284948/101‑1 (MQ=255)
                                        cTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCC..CGGCCAGCGCCCGCCCCGCCCATAGCggg                                                            >  2:2071186/1‑101 (MQ=255)
                                         tGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCC..CGGCCAGCGCCCGCCCCGCCCATAGCGGgc                                                           >  1:4194799/1‑101 (MQ=255)
                                         tGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCC..CGGCCAGCGCCCGCCCCGCCCATAGCGGgc                                                           <  2:782135/101‑1 (MQ=255)
                                             ttGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCC..CGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCtt                                                       >  1:843136/1‑101 (MQ=255)
                                              tGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCC..CGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTtc                                                      >  2:2757302/1‑101 (MQ=255)
                                              tGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCC..CGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTtc                                                      >  2:113902/1‑101 (MQ=255)
                                               ggCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCC..CGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTtct                                                     >  2:2324470/1‑101 (MQ=255)
                                               ggCCGCGGCCGGTGACGCGGCCATGCGAGACATCCGCAGTCACCACCGCCGGGGACCAAAGTCCC..CGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTtct                                                     <  1:4249479/101‑1 (MQ=255)
                                                gCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCC..CGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTtctc                                                    <  1:2393281/101‑1 (MQ=255)
                                                 ccGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCC..CGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCTcg                                                   >  2:1107400/1‑101 (MQ=255)
                                                        cGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCC..CGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCTCGCccgccg                                            >  2:3077769/1‑101 (MQ=255)
                                                         ggTGACGCGGCCATGCGAGACATCTGCAGTCCCCACCGCCGGGGACCAAAGCCCC..CGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCTCGCCCGCCgg                                           <  1:1745641/101‑1 (MQ=255)
                                                         ggTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCC..CGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCTCGCCCGCCgg                                           >  2:1438939/1‑101 (MQ=255)
                                                          gTGACGCGGCCATGCGAGACATCTGCAGTCCCCACCGCCGGGGACCAAAGTCCC..CGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCTCGCCCGCCggg                                          <  2:4078603/101‑1 (MQ=255)
                                                           tGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCC..CGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCTCGCCCGCCgggg                                         >  1:3257949/1‑101 (MQ=255)
                                                           ggACGCGGCCATGCGAGACATCTGCAGTCCCCACCGCCGGGGACCAAAGTCCC..CGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCTCGCCCGCCgggg                                         <  1:2071326/100‑1 (MQ=255)
                                                            gccgcgGCCAGGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCC..CGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCTCGCCCGCCGGGGc                                        <  2:3753614/99‑1 (MQ=255)
                                                             ccgcgGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCC..CGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCTCGCCCGCCGGGGCa                                       <  1:3215903/100‑1 (MQ=255)
                                                                cgGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCC..CGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCTCGCCCGCCGGGGCAGgc                                    >  1:587500/1‑101 (MQ=255)
                                                                cgGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCC..CGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCTCGCCCGCCGGGGCAGgc                                    >  1:170965/1‑101 (MQ=255)
                                                                 ggCCAGGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCC..CGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCTCGCCCGCCGGGGCAGgcg                                   <  2:82879/101‑1 (MQ=255)
                                                                 cgCCATGCGCGGCATCTGCGTCCCCCGCCGCCGGGGCCGGAACTCCC..CGTCCGGCGCCCGCCCCGCCCATAGCGGGCGCTTCTCGCCCGCCGGGGCAGgcg                                   <  1:631489/100‑1 (MQ=12)
                                                                     aTGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCC..CGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCTCGCCCGCCGGGGCAGGCGCCgg                               >  1:2203778/1‑101 (MQ=255)
                                                                          agccATCTGCGGCCACCACCGCGGGGCACCAAAGCCCC..CGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCTCGCCCGCCGGGGCAGGCGCCGGCctct                          <  2:3289864/98‑1 (MQ=17)
                                                                          agaCATCTGCAGCCACCACCGCCGGGGACCAAAGCCCC..CGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCTCGCCCGCCGGGGCAGGCGCCGGCctct                          <  2:2951118/101‑1 (MQ=255)
                                                                           gaCATCTGCAGTCACCACCGCCGGGGACCAAAGTCCC..CGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCTCGCCCGCCGGGGCAGGCGCCGGCCTCTg                         >  2:2657096/1‑101 (MQ=255)
                                                                               tCTGCAGTCACCACCGCCGGGGACCAAAGTCCC..CGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCTCGCCCGCCGGGGCAGGCGCCGGCCTCTGTTgc                     >  2:4413570/1‑101 (MQ=255)
                                                                                cTGCAGTCACCACCGCCGGGGACCAAAGTCCC..CGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCTCGCCCGCCGGGGCAGGCGCCGGCCTCTGTTgcg                    >  1:3136007/1‑101 (MQ=255)
                                                                                cTGCAGCCCCCACCGCCGGGGACCAAAGCCCC..CGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCTCGCCCGCCGGGGCAGGCGCCGGCCTCTGTTgcg                    <  1:3006180/101‑1 (MQ=255)
                                                                                 tGCAGTCACCACCGCCGGGGACCAAAGTCCC..CGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCTCGCCCGCCGGGGCAGGCGCCGGCCTCTGTTgcgc                   >  2:3370012/1‑101 (MQ=255)
                                                                                  gcatccaccccCGCCGGGCACCAAATCCCC..CGGCCAGCGCCCGCCCCGCCCATAGCGGTCGCTTCTCGCCCGCCGGGGCAGGCGCCGGCCTCTGTTgcgcg                  <  2:613276/96‑1 (MQ=17)
                                                                                  gCAGTCACCACCGCCGGGGACCAAAGTCCC..CGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCTCGCCCGCCGGGGCAGGCGCCGGCCTCTGTTgcgcg                  >  2:1224451/1‑101 (MQ=255)
                                                                                  gCAGTCACCACCGCCGGGGACCAAAGTCCC..CGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCTCGCCCGCCGGGGCAGGCGCCGGCCTCTGTTgcgcc                  >  2:3123860/1‑100 (MQ=255)
                                                                                   cAGTCCCCCCCGCCGGGGACCAAAGCCCC..CGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCTCGCCCGCCGGGGCAGGCGCCGGCCTCTGTTgcgcgc                 <  1:275913/101‑1 (MQ=255)
                                                                                   cAGTCACCACCGCCGGGGACCAAAGTCCC..CGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCTCGCCCGCCGGGGCAGGCGCCGGCCTCTGGTgcgcgc                 >  2:4293623/1‑101 (MQ=255)
                                                                                    cgTCCCCCCCGCCGGGTACCAAAGCCCC..CGNCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCTCGCCCGCCGGGGCAGGCGCCGGCCTCTGTTGcgcgcg                <  1:54794/100‑1 (MQ=17)
                                                                                    cctcggccccgcggGGGCCCAAAGGCCCCTCGCCCGGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCGGGCGCCGGCCTCTGCTgcgcc                  <  2:3210647/93‑2 (MQ=11)
                                                                                    aGTCACCACCGCCGGGGACCAAAGTCCC..CGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCTCGCCCGCCGGGGCAGGCGCCGGCCTCTGTTGcgcgcg                >  2:1916288/1‑101 (MQ=255)
                                                                                    aGTCACCACCGCCGGGGACCAAAGTCCC..CGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCTCGCCCGCCGGGGCAGGCGCCGGCCTCTGGTGcccgcg                >  1:3450732/1‑101 (MQ=255)
                                                                                    aGTCACCACCGCCGGGGACCAAAGTCCC..CGGCCAGCGCCCGCCCCCCCCATAGCGGGGGCTTCTCGCCCGCCGGGGCAGGCGCCGGCCCCTGTTgcgcggg                >  1:1235042/1‑99 (MQ=255)
                                                                                     gtccccccggccgGGGACAAAGGCCCC..CGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCTCGCCCGCCGGGGCAGGCGCCGGCCTCTGTTGCGCGCgg               <  2:1714635/92‑1 (MQ=14)
                                                                                      tCACCACCGCCGGGGACCAAAGTCCC..CGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCTCGCCCGCCGGGGCAGGCGCCGGCCTCTGTTGCGCGCggg              >  1:1366013/1‑101 (MQ=255)
                                                                                        ccccccgccgGGGACCAAAGCCCC..CGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCTCGCCCGCCGGGGCAGGCGCCGGCCTCTGTTGCGCGCGGGGc            <  1:706124/97‑1 (MQ=255)
                                                                                        ccccccgccgGGGACCAAAGCCCC..CGCCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCTCGCCCGCCGGGGCAGGCGCCGGCCTCTGTTGCGCGCGGGGc            <  1:1999848/97‑1 (MQ=255)
                                                                                        accaccGCCGGGGACCAAAGTCCC..CGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCTCGCCCGCCGGGGCAGGCGCCGGCCTCTGTTGCGCGCGGGGc            >  1:3343939/1‑101 (MQ=255)
                                                                                        accaccGCCGGGGACCAAAGTCCC..CGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCTCGCCCGCCGGGGCAGGCGCCGGCCTCTGTTGCGCGCGGGGc            >  2:2026762/1‑101 (MQ=255)
                                                                                        accaccGCCGGGGACCAAAGTCCC..CGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCTCGCCCGCCGGGGCAGGCGCCGGCCCCTGTTG.GGGCgggggg           >  1:3493846/1‑99 (MQ=18)
                                                                                         ccacgGCCGGGGCCCAAAGCCCC..CGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCTCGCCCGCCGGGGCAGGCGCCGGCCTCTGTTGCGCGCGGGGCa           <  2:2489984/101‑1 (MQ=255)
                                                                                         ccaccGCCGGGGACCAAAGTCCC..CGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCTCGCCCGCCGGGGCAGGCGCCGGCCTCTGTTGCGCGCGGGGCa           >  1:3785372/1‑101 (MQ=255)
                                                                                            ccgcggGGGACCAAAGCCCC..CGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCTCGCCCGCCGGGGCAGGCGCCGGCCTCTGTTGCGCGCGGGGCAGAc        <  1:2108787/101‑1 (MQ=255)
                                                                                             cgccgAGGACCAAAGCCCC..GGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCTCGCCCGCCGGGGCAGGCGCCGGCCTCTGTTGCGCGCGGGGCAGACg       <  2:664559/101‑1 (MQ=255)
                                                                                              gcgggggCCCAAAGCCCC..CGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCTCGCCCGCCGGGGCAGGCGCCGGCCTCTGTTGCGCGCGGGGCAGACgg      <  1:3350413/98‑1 (MQ=255)
                                                                                               ccgGGGACCAAAGCCCC..CGGCCGGCGCCCGCCCCGCCCATAGCGGGCGCTTCTCGCCCGCCGGGGCAGGCGCCGGCCTCTGTTGCGCGCGGGGCAGACGGt     <  1:4530870/101‑1 (MQ=255)
                                                                                               ccgGGGACCAAAGCCCC..CGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCTCGCCCGCCGGGGCAGGCGCCGGCCTCTGTTGCGCGCGGGGCAGACGGt     <  1:3705427/101‑1 (MQ=255)
                                                                                                cgGGGACCAAAGCCCC..CGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCTCGCCCGCCGGGGCAGGCGCCGGCCTCTGTTGCGCGCCGGGCAGACGGTc    <  1:2521200/101‑1 (MQ=255)
                                                                                                 ggggACCAAAGTCCC..CGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCTCGCCCGCCGGGGCAGGCGCCGGCCTCTGTTGCGCGCGGGGCAGACGGTCt   >  2:3797808/1‑101 (MQ=255)
                                                                                                 ggggACCAAAGCCCC..CGGCCGGCGCCCGCCCCGCCCATAGCGGGCGCTTCTCGCCCGCCGGGGCAGGCGCCGGCCTCTGTTGCGCGCGGGGCAGACGGTCt   <  1:2876800/101‑1 (MQ=255)
                                                                                                                                                                                                         
CCCGCCTTGGTCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCC..CGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCTCGCCCGCCGGGGCAGGCGCCGGCCTCTGTTGCGCGCGGGGCAGACGGTCTT  >  NC_014034/2953218‑2953416

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 23 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG