Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 296,280 +T 2.4% intergenic (‑80/‑203) RCAP_rcc00258 ← / → RCAP_rcc00259 lipoprotein/porin family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034296,2800.→T 2.4% 4.8 ‑0.6 ‑0.2182intergenic (‑80/‑203)RCAP_rcc00258/RCAP_rcc00259lipoprotein/porin family protein
Reads supporting (aligned to +/- strand):  new base (1/2):  ref base (103/74):  total (104/78)
Fisher's exact test strand distribution p-value = 5.74e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.06e-01

Only 100 of 218 total aligned reads displayed.

CAAAGAGGTTGCTTTCTTCATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTTCCCCCCGCGGGACCGCCCGC.GGCGGGGCGCAACCTCCCGCCGGTCCCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGTTTCGCTTTGGCCGCTTCG  >  NC_014034/296180‑296379
                                                                                                                                                                                                         
cAAAGAGGTTGCTTTCTTCATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTTc                                                                                                      >  1:4353711/1‑101 (MQ=255)
 aaaGAGGTTGCTTTCTTCATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTTcc                                                                                                     <  2:1044080/101‑1 (MQ=255)
 aaaGAGGTTGCTTTCTTCATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTTcc                                                                                                     >  2:1646112/1‑101 (MQ=255)
     agGTTGCTTTCTTCATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTTcccccg                                                                                                 >  1:4475295/1‑100 (MQ=255)
     agGTTGCTTTCTTCATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTTcccccg                                                                                                 <  2:2182697/101‑2 (MQ=255)
     agGTTGCTTTCTTCATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTTcccccg                                                                                                 >  2:3337310/1‑100 (MQ=255)
     agGTTGCTTTCTTCATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTTcccccg                                                                                                 >  2:400121/1‑100 (MQ=255)
      ggTTGCTTTCTTCATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTTcccccgc                                                                                                >  1:3804060/1‑99 (MQ=255)
      ggTTGCTTTCTTCATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTTcccccgc                                                                                                >  1:2687202/1‑99 (MQ=255)
        ttGCTTTCTTCATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGCTTTTTcccccgcgg                                                                                              >  1:2981356/1‑97 (MQ=255)
        ttGCTTTCTTCATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTTcccccgcgg                                                                                              >  2:906794/1‑97 (MQ=255)
        ttGCTTTCTTCATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTTcccccgcgg                                                                                              <  1:82306/101‑5 (MQ=255)
         tGCTTTCTTCATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTTcccccccggg                                                                                             >  2:2417535/1‑97 (MQ=255)
           cTTTCTTCATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGAc                                                                                          <  1:2345286/101‑1 (MQ=255)
            tttCTTCATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCTGGAcc                                                                                         >  2:1014103/1‑101 (MQ=255)
            tttCTTCATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGAcc                                                                                         <  2:3704367/101‑1 (MQ=255)
             ttcttcATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGAccg                                                                                        >  1:2893947/1‑101 (MQ=255)
              tcttcATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGAccgc                                                                                       >  1:1891467/1‑101 (MQ=255)
               cttcATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGAccgcc                                                                                      >  1:4186100/1‑101 (MQ=255)
               cttcATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGAccgcc                                                                                      >  1:1487906/1‑101 (MQ=255)
               cttcATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGAccgcc                                                                                      <  1:2209419/101‑1 (MQ=255)
                 tcATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGAccgcccg                                                                                    >  2:4519086/1‑101 (MQ=255)
                  cATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGAccgcccgc                                                                                   >  2:708421/1‑101 (MQ=255)
                  cATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGAccgcccgc                                                                                   >  1:1601766/1‑101 (MQ=255)
                   aTACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCCCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCcac.g                                                                                 >  2:759440/1‑101 (MQ=255)
                     aCGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCgc.ggc                                                                               <  1:1147487/101‑1 (MQ=255)
                       gATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGc.ggcgg                                                                             >  2:2963337/1‑101 (MQ=255)
                        aTCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGC.GGCggg                                                                            >  2:4162917/1‑101 (MQ=255)
                          cGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGC.GGCGGGgc                                                                          >  2:3673856/1‑101 (MQ=255)
                           gACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCCGTCTCTAGCCGATGGCCGGGGGGGCGACACGATTTTT.CCCCCGCGGGACGCCCCGC.GGCGGGccg                                                                         >  2:1807621/1‑101 (MQ=255)
                             cTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGC.GGCGGGGCGCa                                                                       <  1:606619/101‑1 (MQ=255)
                              tGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGCGGGCGGGCCGCa                                                                       >  2:1788690/1‑101 (MQ=255)
                              tGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGC.GGCGGGGCGCaa                                                                      >  1:3458849/1‑101 (MQ=255)
                               gCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGC.GGCGGGGCGCAAc                                                                     >  1:452267/1‑101 (MQ=255)
                               ccccccCCAGGCTGAACCCATGGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGC.GGCGGGGCGCAAc                                                                     <  2:256075/100‑1 (MQ=255)
                                 ccccGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGC.GGCGGGGCGCAACCt                                                                   <  2:1601661/101‑1 (MQ=255)
                                 ccccGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGC.GGCGGGGCGCAACCt                                                                   >  2:3371251/1‑101 (MQ=255)
                                  cccGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGC.GGCGGGGCGCAACCTc                                                                  >  1:469514/1‑101 (MQ=255)
                                  cccGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGC.GGCGGGGCGCAACCTc                                                                  <  1:975951/101‑1 (MQ=255)
                                    cGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGC.GGCGGGGCGCAACCTccg                                                                <  1:3905400/101‑2 (MQ=255)
                                     gCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCAGCGGGACCGCCCGC.GGCGGGGCGCAACCTccgc                                                               >  1:4024732/1‑99 (MQ=255)
                                      cAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGC.GGCGGGGCGCAACCTccgcc                                                              >  2:4066128/1‑98 (MQ=255)
                                      cAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGC.GGCGGGGCGCAACCTccgcc                                                              <  1:3720893/101‑4 (MQ=255)
                                      cAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGC.GGCGGGGCGCAACC.Cccgcc                                                             >  1:4111567/1‑101 (MQ=255)
                                       aGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGC.GGCGGGGCGCAACCTccgccg                                                             >  1:3155295/1‑97 (MQ=255)
                                          cTGACCCCATGGGCTTTCCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGC.GGCGGGGCGCAACCT.CCGCCGGTc                                                         <  2:3818331/101‑1 (MQ=255)
                                           tGACCCCATTGGCTTTTCCTTCCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGC.GGCGGGGCGCAACCT.CCGCCGGTcc                                                        <  2:1172334/101‑1 (MQ=255)
                                           tGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGC.GGCGGGGCGCAACCT.CCGCCGGTcc                                                        >  2:1978168/1‑101 (MQ=255)
                                            gAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGC.GGCGGGGCGCAACCT.CCGCCGGTccc                                                       >  1:2622558/1‑101 (MQ=255)
                                             aaCCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGC.GGCGGGGCGCAACCT.CCGCCGGTccca                                                      >  2:2059633/1‑100 (MQ=255)
                                              aCCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGC.GGCGGGGCGCAACCT.CCGCCGGTcccag                                                     >  1:907368/1‑99 (MQ=255)
                                              aCCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGC.GGCGGGGCGCAACCT.CCGCCGGTcccag                                                     >  2:4377078/1‑99 (MQ=255)
                                               ctctttGGCTTTCCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGC.GGCGGGGCGCAACCT.CCGCCGGTcccagc                                                    <  2:2446367/97‑4 (MQ=255)
                                                ccATGGGTTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGC.GGCGGGGCGCAACCT.CCGCCGGTcccagcg                                                   <  1:1014186/101‑5 (MQ=255)
                                                 cATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGC.GGCGGGGCGCAACCT.CCGCCGGTcccagcag                                                  >  1:2446533/1‑96 (MQ=255)
                                                  aTTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGC.GGCGGGGCGCAACCT.CCGCCGGT.CCCAGCggg                                                >  2:1351704/1‑101 (MQ=255)
                                                   ttGTCTTTCCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGC.GGCGGGGCGCAACCT.CCGCCGGT.CCCAGCgggg                                               <  1:3616463/101‑1 (MQ=255)
                                                   ttGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGC.GGCGGGGCGCAACCT.CCGCCGGT.CCCAGCgggg                                               >  2:192151/1‑101 (MQ=255)
                                                    tGGCTTTTCCGGTTTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGC.GGCGGGGCGCAACCT.CCGCCGGT.CCCAGCggggg                                              <  1:3991714/101‑1 (MQ=255)
                                                     tgCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGC.GGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGa                                             <  2:2096874/100‑1 (MQ=255)
                                                     ggCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGC.GGCGGGGCGCAACCT.CCGCCGGTCCCC.GCGGGGGa                                             >  2:4547979/1‑101 (MQ=255)
                                                     ggCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGC.GGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGa                                             <  2:2890294/101‑1 (MQ=255)
                                                     ggCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGC.GGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGa                                             >  1:3306617/1‑101 (MQ=255)
                                                       cTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGC.GGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACt                                           <  2:1806105/101‑1 (MQ=255)
                                                        ttttCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGC.GGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGGCtg                                          >  1:1932289/1‑101 (MQ=255)
                                                         tttCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGC.GGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACtgt                                         <  1:2952088/101‑1 (MQ=255)
                                                         tttCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGC.GGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACtgt                                         >  2:3614045/1‑101 (MQ=255)
                                                         tttCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGC.GGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACtgg                                         >  2:1684761/1‑100 (MQ=255)
                                                          ttCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGC.GGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACtgtg                                        >  2:1274717/1‑101 (MQ=255)
                                                               gTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGC.GGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCaaa                                   >  2:714934/1‑101 (MQ=255)
                                                                  tctAGCCGATGGCCGGGGGGGGGACAAGATTTTT.CCCCCGCGGGAACGCCCGC.GGCGGGGCGCACCCTCGC.CCGG.CCCCAGCGGGGGACCGGGGCACAggt                                >  2:3240265/1‑99 (MQ=255)
                                                                  tctAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGC.GGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCt                                >  2:509687/1‑101 (MQ=255)
                                                                  tctAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGC.GGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCt                                >  2:4244846/1‑101 (MQ=255)
                                                                  tctAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGC.GGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCt                                >  1:3013946/1‑101 (MQ=255)
                                                                   ctAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGC.GGCGGGGCGCAACCT.CCGCCGG.GCCCAGCGGGGGACTGTGGCAAAGCTg                               >  2:3887130/1‑101 (MQ=255)
                                                                    tAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGC.GGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGc                              <  2:2120040/101‑1 (MQ=255)
                                                                    tAGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGC.GGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGc                              >  2:3088770/1‑101 (MQ=255)
                                                                     aGCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGC.GGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGca                             >  2:3525280/1‑101 (MQ=255)
                                                                      gCCGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGC.GGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGcac                            <  2:3555304/101‑1 (MQ=255)
                                                                        cGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGC.GGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGcacca                          >  1:1400923/1‑101 (MQ=255)
                                                                        cGATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGC.GGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGcacca                          >  2:3943922/1‑101 (MQ=255)
                                                                         gATGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGC.GGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGcaccac                         <  2:4232713/101‑1 (MQ=255)
                                                                          aTGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGC.GGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCAcg                        >  2:2691914/1‑101 (MQ=255)
                                                                           tGGCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGC.GGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCAcgc                       >  2:4268521/1‑101 (MQ=255)
                                                                             gCCGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGC.GGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCgg                     >  2:796004/1‑101 (MQ=255)
                                                                               cGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGC.GGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCCAAGCTGCACCACGCgggt                   >  2:3053841/1‑99 (MQ=255)
                                                                               cGGGGGGGCGACAAGATTTTT.CCCCCGCGGGACCGCCCGC.GGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGtt                   >  1:1775425/1‑101 (MQ=255)
                                                                                gggggggCGACAAGATTTTT.CCCCCGCGGGACCGCCCGC.GGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGttt                  <  2:2508016/101‑1 (MQ=255)
                                                                                gggggggCGACAAGATTTTT.CCCCCGCGGGACCGCCCGC.GGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGttt                  <  1:311485/101‑1 (MQ=255)
                                                                                 ggggggCGACAAG.GTTTTCCCCCCGCGGGACCGCCCGC.GGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGTTTc                 <  2:1218594/101‑1 (MQ=255)
                                                                                  gggggCGACAAGATTTTT.CCCCCGCGGGACCGCCCGC.GGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGTTTCg                >  1:1322205/1‑101 (MQ=255)
                                                                                  gggggCGACAAGATTTTT.CCCCCGCGGGACCGCCCGC.GGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGTTTCg                <  1:1422694/101‑1 (MQ=255)
                                                                                  gggggCGACAAGATTTTT.CCCCCGCGGGACCGCCCGC.GGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGTTTCg                <  2:2622373/101‑1 (MQ=255)
                                                                                  gggggCGACAAGATTTTT.CCCCCGCGGGACCGCCCGC.GGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGTTTCg                >  1:3502520/1‑101 (MQ=255)
                                                                                      gCGACAAGATTTTT.CCCCCGCGGGACCGCCCGC.GGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGTTTCGCttt            <  2:1125462/101‑1 (MQ=255)
                                                                                       cGACAAGATTTTT.CCCCCGCGGGACCGCCCGC.GGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGTTTCGCTTTg           <  1:3674131/101‑1 (MQ=255)
                                                                                       cGACAAGATTTTT.CCCCCGCGGGACCGCCCGC.GGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGTTTCGCTTTg           <  2:4054377/101‑1 (MQ=255)
                                                                                           aaGATTTTT.CCCCCGCGGGACCGCCCGC.GGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGTTTCGCTTTGGCCg       >  1:2815094/1‑101 (MQ=255)
                                                                                               attttTCCCCCGCGGGACCGCCCGC.GGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGTTTCGCTTTGGCCGCtt    <  2:550000/100‑1 (MQ=255)
                                                                                                 tttTCCCCCGCGGGACCGCCCGC.GGCGGGGCGCAACCT.CCGCCGGT.CCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGTTTCGCTTTGGCCGCTTCg  <  1:4243231/101‑1 (MQ=255)
                                                                                                                                                                                                         
CAAAGAGGTTGCTTTCTTCATACGATCGACTGCCCCCGCAGGCTGAACCCATTGGCTTTTCCTGTCTCTAGCCGATGGCCGGGGGGGCGACAAGATTTTTCCCCCCGCGGGACCGCCCGC.GGCGGGGCGCAACCTCCCGCCGGTCCCCAGCGGGGGACTGTGGCAAAGCTGCACCACGCGGTTTCGCTTTGGCCGCTTCG  >  NC_014034/296180‑296379

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG