Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,995,643 +C 6.2% intergenic (+183/+37) RCAP_rcc02802 → / ← RCAP_rcc02803 membrane dipeptidase/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,995,6431.→C 6.2% 3.5 0.0 ‑0.448intergenic (+183/+37)RCAP_rcc02802/RCAP_rcc02803membrane dipeptidase/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (3/0):  ref base (37/8):  total (40/8)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.67e-01

CCCTTCGGTTGCCGCCCCGCAACCCGTCGGCGGCCCAGCGCCCTGCTTTTCCTTGG.CAAAATACG.CCGGGGGGTGCGGGGGGCGGAGCCCCCCG.CGGGTCGCCCCGGCTT..GCCGGGGCGAAATCCCCCGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGACGCGGATGCGCAGGCGGGCCGAAAGC  >  NC_014034/2995550‑2995742
                                                                                                |                                                                                                     
cccTTCGGTTGCCGCCCCGCAACCCGTCGGCGGCCCAGCGCCCTGCTTTTCCTTGG.CAAAATACG.CCGGGGGGTGCGGGGGGCGGAGCCCCCCG.CGGGGCg                                                                                                >  1:2403200/1‑101 (MQ=255)
    tCGGTTGCCGCCCCGCACCCCGTCGGCGGCCCGGCGCCCTGCTTTTCCTTGG.AAAAATCCG.CGGGGGGGTGGGGGGGGGGGAGCCCCCCG.CGGGTCGcccc                                                                                            <  2:3010775/101‑1 (MQ=21)
     cGGTTGCCGCCCCGCAACCCGTCGGCGGCCCAGCGCCCTGCTTTTCCTTGG.CAAAATACG.CCGGGGGGTGCGGGGGGCGGAGCCCCCCG.CGGGGCGCCCCg                                                                                           >  1:1951017/1‑101 (MQ=255)
            cgccCCGCAACCCGTCGGCGGCCCAGCGCCCTGCTTTTCCTTGG.CAAAATACG.CCGGGGGGTGCGGGGGGCGGAGCCCCCCG.CGGGACGCCCCGGCttttc                                                                                    >  2:2815451/1‑98 (MQ=255)
                  gCACCCCGTCGGCGGCCCAGCGCCCTGCTTTTCCTTGG.CAAAATACG.CCGGGGGGTGCGGGGGGCGGAGCCCCCCG.CGGGTCGCCCCGGCTT..GCCGGGGCg                                                                            <  2:2877615/101‑1 (MQ=255)
                         gTCGGCGGCCCAGCGCCCTGCTTTTCCTTGG.CAAAATACG.CCGGGGGGTGCGGGGGGCGGAGCCCCCCG.CGGGTCGCCCCCGCTT..GCCGGGGGGAAATccc                                                                     >  1:987598/1‑101 (MQ=255)
                            ggcggcCCAGCGCCCTGCTTTTCCTTGG.CAAAATACG.CCGGGGGGGGCGGGGGGCGGAGCCCCCCG.CGGGTCGCCCCGGCTT..GCCGGGGCGAAATCCCCCg                                                                  >  1:4607369/1‑101 (MQ=255)
                               ggcCCGGCCCCCTGCTTTCCTGTGC.AAAAATCGG.CGGGGGGGTGCGGGGGGGGGGCCCCCCCG.CGGGTCGCCCCGGCTT..GCCGGGGCGAAATCCCCCGCTc                                                               <  2:4436225/101‑1 (MQ=16)
                                 cccAGCGCCCT.CTTTTCCTTGGACAAAATGCG.CGGGGGGGTGGGGGGGGGGGGCCCCCCCG.CGGGTCGCCCCGGCTT..GCCGGGGCGAAATCCCCCGCTCAg                                                             <  2:403594/101‑1 (MQ=25)
                                  ccAGCGCCCTGCTTTTCCTTGG.CAAAATACG.CCGGGGGGTGCGGGGGGCGGAGCCCCCCG.CGGGTCGCCCCGGCTT..GCCGGGGCGAAATCCCCCGCTCAGt                                                            >  2:1417389/1‑101 (MQ=255)
                                     cggctttgtttttCCTTGG.AAAAATACGCCCGGGGGGTGCGGGGGGCGGAGCCCCCCGCCGGGTCGCCTCGGCGTCAGCCGAGGCGAAATACCCC.CTCAgc                                                            >  2:2260547/10‑100 (MQ=0)
                                       gcCCTGCTTTTCCTTGG.CAAAATACG.CCGGGGGGTGCGGGGGGCGGAGCCCCCCG.CGGGGCGCCCCGGCTT..GCCCGGGCGAAATCCCCCGCCCAATCCCag                                                       >  2:1915329/1‑101 (MQ=18)
                                            gtttttCCTTGG.AAAAATACGCCCGGGGGGTGCGGGGGGCGGAGCCCCCCGCCGGGTCGCCTCGGCGTCAGCCGAGGCGAAATACCCC.CTCAGCccttcat                                                     >  1:1058553/3‑96 (MQ=0)
                                              ttttCCTTGG.CAAAATACG.CCGGGGGGTGCGGGGGGCGGAGCCCCCCG.CGGGTCGCCCCGGCTT..GCCGGGGCGAAATCCCCCGCTCAGTCCCAGAGACttt                                                >  1:2991227/1‑101 (MQ=255)
                                               tttCCTGGG.CAAGAGACG.GCGGGGGGGGGGGGGGGGGCGGGCCCCCG.CGGGTCGCCCCGGCTT..GCCGGGGCGAAATCCCCCGCTCAGTCCCAGAGACTTTc                                               <  1:1362820/101‑1 (MQ=25)
                                                ttCCTTGG.CAAAATACG.CCGGGGGGTGCGGGGGGCGGAGCCCCCCG.CGGGGCGCCCCGGCTT..GCCGGGGCGAAATCCCCCGCTCCGTCCCCCAGACTTTCg                                              >  1:2053905/1‑101 (MQ=21)
                                                 tCCTTGG.AAAAATACGCCCGGGGGGTGCGGGGGGCGGAGCCCCCCGCCGGGTCGCCTCGGCGTCAGCCGAGGCGAAATACCCC.CTCAGCccttcatcttca                                                >  2:3659601/1‑91 (MQ=0)
                                                    ctggaaaaaaTCCG.GGGGGGGGGGGGGGGGGCGGTCCCCCCCG.CGGGTCGCCCCGGCTT..GCCGGGGCGAAATCCCCCGCTCAGTCCCAGAGACTTTCGAcc                                           <  1:3123620/95‑1 (MQ=25)
                                                      gg.CAAAATACG.CCGGGGGGTGCGGGGGGCAGAGCCCCCCG.CGGGTCGCCCCGGCTT..GCCGGGGCGAAATCCCCCGCTCAGTCCCAGAGACTTTCGACCTCg                                        >  1:4528715/1‑101 (MQ=255)
                                                            aaTACG.CCGGGGGGTGCGGGGGGCGGAGCCCCCCG.CGGGTCGCCCCGGCTT..GCCGGGGCGAAATCCCCCGATCAGTCCCCGAGACTTTCACCCTCGAACCg                                   >  2:1088520/1‑101 (MQ=25)
                                                              tACG.CCGGGGGGTGCGGGGGGCGGAGCCCCCCG.CGGGCCGCCCCGGCCT..GGCGGGGCGAAAACCCCCGCTCAGTCCCCGAGACTTTCGACCTCGAACCggg                                 >  1:3081407/1‑101 (MQ=37)
                                                                       ggggTGCGGGGGGCGGAGCCCCCCG.CGGGTCGCCCCGGCTT..GCCGGGGCGAAATCCCCCGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGAcgc                         >  1:596885/1‑101 (MQ=255)
                                                                        gggTGCGGGGGGCGGAGCCCCCCG.CGGGTCGCCCCGGCTT..GCCGGGGCGAAATCCCCCGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGAcgcg                        >  1:1300790/1‑101 (MQ=255)
                                                                          gTGCGGGGGGCGGAGCCCCCCG.CGGGTCGCCCCGGCTT..GCCGGGGCGAAATCCCCCGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGCCGCCGa                      >  2:952220/1‑101 (MQ=255)
                                                                            gCGGGGGGCGGAGCCCCCCG.CGGGTCGCCCCGGCTT..GCCGGGGCGAAATCCCCCGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGACGCGGATg                    >  1:1771106/1‑101 (MQ=255)
                                                                            gCGGGGGGCGGAGCCCCCCG.CGGGTCGCCCCGGCTT..GCCGGGGCGAAATCCCCCGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGACGCGGATg                    <  1:199170/101‑1 (MQ=255)
                                                                            gCGGGGGGCGGAGCCCCCCG.CGGGTCGCCCCGGCTT..GCCGGGGCGAAATCCCCCGCTCAGTCCCACAGAATTTCGACCTCGAACCGGGGCAGACGCGGATg                    >  2:602670/1‑101 (MQ=255)
                                                                             cGGGGGGCGGAGCCCCCCG.CGGGTCGCCCCGGCTT..GCCGGGGCGAAATCCCCCGCTCAATCCCAGAGACTTTCGACCTCGCACCCGGCCCGACGCGGATgc                   >  2:2518057/1‑101 (MQ=38)
                                                                              ggggggCGGAGCCCCCCG.CGGGTCGCCCCGGCTT..GCCGGGGCGAAATCCCCCGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGACGCGGATgcg                  >  1:3097920/1‑101 (MQ=255)
                                                                              ggggggCGGAGCCCCCCG.CGGGTCGCCCCGGCTT..GCCGGGGCGAAATCCCCCGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGACGCGGATgcg                  >  2:3504661/1‑101 (MQ=255)
                                                                              ggggggCGGAGCCCCCCG.CGGGTCGCCCCGGCTT..GCCGGGGCGAAATCCCCCGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGACGCGGATgcg                  >  2:3420874/1‑101 (MQ=255)
                                                                              ggggggCGGAGCCCCCCG.CGGGTCGCCCCGGCTT..GCCGGGGCGAAATCCCCCGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGGCAGACGCGGATgcg                  >  1:1270322/1‑101 (MQ=255)
                                                                               gggggCGGAGCCCCCCG.CGGGTCGCCCCGGCTT..GCCGGGGCGAAATCCCCCGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGACGCGGATgcgc                 >  2:3386575/1‑101 (MQ=255)
                                                                               gggggCGGAGCCCCCCG.CGGGTCGCCCCGGCTT..GCCGGGGCGAAATCCCCCGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGGCAGACGCGGATgcgc                 >  2:392222/1‑101 (MQ=255)
                                                                                  ggCGGAGCCCCCCG.CGGGTCGCCCCGGCTT..GCCGGGGCGAAATCCCCCGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGACGCGGATGCGCAgg              >  1:2403843/1‑101 (MQ=255)
                                                                                  ggCGGAGCCCCCCG.CGGGTCGCCCCGGCTT..GCCGGGGCGAAATCCCCCGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGACGCGGATGCGCAgg              <  1:333612/101‑1 (MQ=255)
                                                                                   gCGGAGCCCCCCG.CGGGTCGCCCCGGCTT..GCCGGGGCGAAATCCCCCGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGACGCGGATGCGCAGGc             >  2:3670598/1‑101 (MQ=255)
                                                                                    cGGAGCCCCCCG.CGGGTCGCCCCGGCTT..GCCGGGGCGAAATCCCCCGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGACGCGGATGCGCAGGCg            >  1:3492674/1‑101 (MQ=255)
                                                                                     ggccccccccG.CGGGTCGCCCCGGCTT..GCCGGGGCGAAATCCCCCGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGACGCGGATGCGCAGGCgg           <  1:2518231/97‑1 (MQ=255)
                                                                                     ggAGCCCCCCG.CGGGTCGCCCCGGCTT..GCCGGGGCGAAATCCCCCGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGACGCGGATGCGCAGGCgg           >  2:3372982/1‑101 (MQ=255)
                                                                                     ggAGCCCCCCG.CGGGTCGCCCCGGCTT..GCCGGGGCGAAATCCCCCGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGACGCGGATGCGCAGGCgg           >  1:2275589/1‑101 (MQ=255)
                                                                                      gAGCCCCCCG.CGGGTCGCCCCGGCTT..GCCGGGGCGAAATCCCCCGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGACGCGGATGCGCAGGCggg          >  2:3109753/1‑101 (MQ=255)
                                                                                      gAGCCCCCCG.CGGGTCGCCCCGGCTT..GCCGGGGCGAAATCCCCCGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGACGCGGATGCGCAGGCggg          >  1:143844/1‑101 (MQ=255)
                                                                                      gAGCCCCCCG.CGGGTCGCCCCGGCTT..GCCGGGGCGAAATCCCCCGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGACGCGGATGCGCAGGCggg          >  2:1258319/1‑101 (MQ=255)
                                                                                       aGCCCCCCG.CGGGTCGCCCCGGCTT..GCCGGGGCGAAATCCCCCGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGACGCGGATGCGCAGGCGGGc         >  2:3306607/1‑101 (MQ=255)
                                                                                       aGCCCCCCG.CGGGTCGCCCCGGCTT..GCCGGGGCGAAATCCCCCGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGACGCGGATGCGCAGGCGGGc         >  2:4376988/1‑101 (MQ=255)
                                                                                        gCCCCCCG.CGGGTCGCCCCGGCTT..GCCGGGGCGAAATCCCCCGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGACGCGGATGCGCAGGCGTGcc        >  1:722063/1‑101 (MQ=255)
                                                                                        gCCCCCCG.CGGGTCGCCCCGGCTT..GCCGGGGCGAAATCCCCCGCCCAGTCCCAGAGACTTTCGACCTCGAACCGGGGCAGACGCGGATGGGCAGGCGGGcc        >  1:884355/1‑101 (MQ=255)
                                                                                           ccccG.CGGGTCGCCCCGGCTT..GCCGGGGCGAAATCCCCCGCTCAGTCCCATAGACTTTCGACCTCGAACCGGGCCAGACGCGGATGCGCAGGCGGGCCGaa     >  2:4589351/1‑101 (MQ=255)
                                                                                            ccgg.cgGGTCGCCCCGGCTT..GCCGGGGCGAAATCCCCCGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGACGCGGATGCGCAGGCGGGCCGaaa    <  2:2403038/98‑1 (MQ=255)
                                                                                            cccG.CGGGTCGCCCCGGCTT..GCCGGGGCGAAATCCCCCGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGACGCGGATGCGCAGGCGGGCCGaaa    >  2:1984235/1‑101 (MQ=255)
                                                                                             ccG.CGGGTCGCCCCGGCTT..GCCGGGGCGAAATCCCCCGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGACGCGGATGCGCAGGCGGGCCGAAAg   >  2:3390873/1‑101 (MQ=255)
                                                                                              cg.cgGGTCGCCCCGGCTT..GCCGGGGCGAAATCCCCCGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGACGCGGATGCGCAGGCGGGCCGAAAGc  >  1:3197355/1‑101 (MQ=255)
                                                                                              cg.cgGGTCGCCCCGGCTT..GCCGGGGCGAAATCCCCCGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGACGCGGATGCGCAGGCGGGCCGAAAGc  >  1:1944243/1‑101 (MQ=255)
                                                                                                |                                                                                                     
CCCTTCGGTTGCCGCCCCGCAACCCGTCGGCGGCCCAGCGCCCTGCTTTTCCTTGG.CAAAATACG.CCGGGGGGTGCGGGGGGCGGAGCCCCCCG.CGGGTCGCCCCGGCTT..GCCGGGGCGAAATCCCCCGCTCAGTCCCAGAGACTTTCGACCTCGAACCGGGTCAGACGCGGATGCGCAGGCGGGCCGAAAGC  >  NC_014034/2995550‑2995742

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 21 ≤  ATCG  < 31 ≤  ATCG  < 39 ≤  ATCG