Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,256,164 +G 100% coding (1307/1734 nt) recJ → single‑stranded‑DNA‑specific exonuclease RecJ

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,256,1641.→G100.0% 660.6 175coding (1307/1734 nt)recJsingle‑stranded‑DNA‑specific exonuclease RecJ
Reads supporting (aligned to +/- strand):  new base (86/89):  ref base (0/0):  total (86/89)

Only 100 of 197 total aligned reads displayed.

TCGGTGGCGGGGGTCGATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCT.GCCGAGG.GCAG.TGGAA.CCGCGATGG.AA.GGCT.GCC.GAG.TGTTCGCCCGGCA..GGGCGC.GGCAC.GGCGGCCCGGCCGATCTGCGGCTGGA  >  NC_014034/3256064‑3256251
                                                                                                     |                                                                                                   
tCGGTGGCGGGGGTCGATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCt                                                                                                      <  1:3314795/101‑1 (MQ=255)
tCGGTGGCGGGGGTCGATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCt                                                                                                      >  2:2880632/1‑101 (MQ=255)
 cGGTGGCGGGGGTCGATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCGGCGGGTCTGACCCT.g                                                                                                    >  1:3048617/1‑101 (MQ=255)
 cGGTGGCGGGGGTCGATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCT.g                                                                                                    <  1:3092264/101‑1 (MQ=255)
 cGGTGGCGGGGGTCGATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCT.g                                                                                                    <  1:4566372/101‑1 (MQ=255)
  ggTGGCGGGGGTCGATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCT.gg                                                                                                   >  2:3394729/1‑100 (MQ=255)
  ggTGGCGGGGGTCGATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCAGGCACCGCATGGCCGCGGGCCTGACCCtcg                                                                                                    >  2:582628/1‑99 (MQ=255)
  ggTGGCGGGGGTCGATCTGGGCGCGGCGGCGACACGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGAACCGCATGGCCGCGGGTCTGGCCCT.gg                                                                                                   >  1:423803/1‑100 (MQ=255)
   gTGGCGGGGGTCGATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCT.ggc                                                                                                  <  1:2169029/101‑3 (MQ=255)
     ggCGGGGGTCGATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCT.ggccg                                                                                                <  2:3856649/101‑5 (MQ=255)
       cGGGGGTCGATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAg                                                                                               <  1:1071691/101‑1 (MQ=255)
        gggggTCGATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAgg                                                                                              >  2:2521128/1‑101 (MQ=255)
           ggTCGATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGCGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATCGCCGCGGGTCTGACCCTCGCCGAgg.ggc                                                                                          >  2:917814/1‑99 (MQ=255)
           ggTCGATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAggcgc                                                                                           <  2:3731373/101‑4 (MQ=255)
           ggTCGATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAggcgc                                                                                           >  2:4339139/1‑98 (MQ=255)
              cGATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAggcgcagc                                                                                        >  1:3818415/1‑95 (MQ=255)
               gATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAggcgcagct                                                                                       <  1:4214072/101‑8 (MQ=255)
               gATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAggcgcagct                                                                                       <  1:2854628/101‑8 (MQ=255)
               gATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAggcgcagct                                                                                       >  1:3458989/1‑94 (MQ=255)
               gATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAggcgcagcg                                                                                       >  2:2159332/1‑94 (MQ=255)
                 tCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAggcgcagctgg                                                                                     >  2:302592/1‑92 (MQ=255)
                  cTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAggcgcagctgga                                                                                    <  2:945352/101‑11 (MQ=255)
                    gggCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAA.c                                                                                 >  1:2628637/1‑101 (MQ=255)
                    gggCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAA.c                                                                                 <  1:4167902/101‑1 (MQ=255)
                    gggCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAA.c                                                                                 >  1:2724437/1‑101 (MQ=255)
                     ggCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAA.cc                                                                                <  2:2371062/101‑1 (MQ=255)
                      gcgcGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAA.ccc                                                                               <  1:3616940/101‑2 (MQ=255)
                      gcgcGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAA.ccc                                                                               <  2:1585600/101‑2 (MQ=255)
                       ggcggcgGTGCAGCGCGTCGGGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAA.cccg                                                                              <  2:1121474/100‑3 (MQ=255)
                       cgcgGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAA.cccg                                                                              <  1:1460638/101‑3 (MQ=255)
                       cgcgGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAA.cccg                                                                              <  2:3527298/101‑3 (MQ=255)
                       cgcgGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAA.cccg                                                                              <  2:1485679/101‑3 (MQ=255)
                        gcggcgGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAA.cccgc                                                                             >  1:2120838/1‑98 (MQ=255)
                        gcggcgGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAA.cccgc                                                                             >  2:2065282/1‑98 (MQ=255)
                        gcggcgGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAA.cccgc                                                                             >  1:706202/1‑98 (MQ=255)
                          ggcggTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAA.cccgcga                                                                           >  1:50996/1‑96 (MQ=255)
                            cggTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATg                                                                          >  1:687618/1‑101 (MQ=255)
                             ggTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATgg                                                                         >  2:1381533/1‑101 (MQ=255)
                             ggTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATgg                                                                         <  2:1569736/101‑1 (MQ=255)
                              gTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATggc                                                                        >  1:1925763/1‑100 (MQ=255)
                                gCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATggcac                                                                      <  2:1053752/101‑4 (MQ=255)
                                 cAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATggcacg                                                                     <  1:1305569/101‑5 (MQ=255)
                                   gcgcGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATggcacggc                                                                   <  2:4303951/101‑7 (MQ=255)
                                    cgcgTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATggcacggct                                                                  <  1:444872/101‑8 (MQ=255)
                                     gcgTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATggcacggctg                                                                 <  2:2509045/101‑9 (MQ=255)
                                     gcgTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATggcacggctg                                                                 <  1:2880848/101‑9 (MQ=255)
                                      cgTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCGTGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATggcacggctgg                                                                <  1:2700696/101‑10 (MQ=255)
                                      cgTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATggcacggctgg                                                                >  1:1754873/1‑92 (MQ=255)
                                      cgTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATggcacggctgg                                                                <  2:1542725/101‑10 (MQ=255)
                                      cgTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATggcacggctgg                                                                >  1:3550169/1‑92 (MQ=255)
                                       gTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATggcacggctggc                                                               >  2:2176497/1‑91 (MQ=255)
                                       gTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATggcacggctggc                                                               >  1:3179744/1‑91 (MQ=255)
                                             gCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGG.CACGGCTGGCC.GAg.tt                                                      <  1:4376935/101‑2 (MQ=255)
                                              ccGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GAg.ttg                                                     >  2:792740/1‑99 (MQ=255)
                                              ccGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GAg.ttg                                                     >  2:1977178/1‑99 (MQ=255)
                                              ccGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGG.CACGGCTGGCC.GAg.ttg                                                     <  2:4397529/101‑3 (MQ=255)
                                               cGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GAg.ttgc                                                    >  1:378386/1‑98 (MQ=255)
                                                gAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GAg.ttgct                                                   >  2:838573/1‑97 (MQ=255)
                                                gAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGCCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GAg.ttgct                                                   >  1:4513077/1‑97 (MQ=255)
                                                 aGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGG.CACGGCTGGCC.GAg.ttgctc                                                  <  2:687556/101‑6 (MQ=255)
                                                  ggggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GAg.ttgctcg                                                 >  1:1838372/1‑95 (MQ=255)
                                                   gggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GAg.ttgctcgc                                                >  1:990426/1‑94 (MQ=255)
                                                   gggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GAg.ttgctcgc                                                >  2:4002870/1‑94 (MQ=255)
                                                    ggCTTTTGGTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GAg.ttgctcgcc                                               <  2:4487207/101‑9 (MQ=255)
                                                    ggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GAg.ttgctcgcc                                               >  2:2086287/1‑93 (MQ=255)
                                                      cTTTTGCTCAAGGGCGGCGGGCACCGCGTGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GAGTTGCTCGCCCg                                              <  2:3305212/101‑1 (MQ=255)
                                                       ttttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GAGTTGCTCGccccg                                             >  2:4563715/1‑99 (MQ=255)
                                                            cTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGG.CACGGCTGGCC.GAGTTGCTCGCCCGGCA..ggg                                      <  2:378349/101‑1 (MQ=255)
                                                            cTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGG.CACGGCTGGCC.GAGTTGCTCGCCCGGCA..ggg                                      <  2:1286207/101‑1 (MQ=255)
                                                             tCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GAGTTGCTCCCCCGGCA..GGgc                                     >  1:2418843/1‑101 (MQ=255)
                                                              cAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GAGTTGCTCGCCCCGCA..GGgcg                                    >  1:3013521/1‑101 (MQ=255)
                                                               aaGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GAGTTGCTCGCCCGGCA..GGgcgg                                   >  2:2787796/1‑100 (MQ=255)
                                                                aGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGG.CACGGCTGGCC.GAGTTGCTCGCCCGGCA..GGGcgc.g                                 <  2:163518/101‑1 (MQ=255)
                                                                 gggCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGGGG.TGCTCGCCCGGCA..GGGCGC.gg                                >  2:4084916/1‑101 (MQ=255)
                                                                 ggacggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GAGTTGCTCGCCCGGCA..GGGGGC.gg                                >  1:2463054/4‑101 (MQ=255)
                                                                    cggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GAGTTGCTCGCCCGGCA..ggggggcggga                              >  1:3241911/1‑93 (MQ=255)
                                                                    cggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GAGTTGCTCGCCCGGCA..GGGCGC.gggca                             <  2:2628452/101‑4 (MQ=255)
                                                                     ggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GAGTTGCTCGCCCGGCA..GGGCGC.gggcga                            >  2:519006/1‑97 (MQ=255)
                                                                     ggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GAGTTGCTCGCCCGGCA..GGGCGC.gggcac                            <  1:4105639/101‑5 (MQ=255)
                                                                       cggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GAGTTGCTCGCCCGGCC..GGGCGC.ggggaagg                          >  2:3095308/1‑95 (MQ=255)
                                                                       cggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GAGTTGCTCGCCCGGCA..GGGCGCGGGCAc.gg                          <  2:4156024/101‑1 (MQ=255)
                                                                        gggCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GAGTTGCTCGCCCGGCA..GGGCGCGGGCAc.ggg                         >  2:2018647/1‑100 (MQ=255)
                                                                         ggCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GAGTTGCTCGCCCGGCA..GGGCGCGGGCAc.gggc                        >  1:3413846/1‑99 (MQ=255)
                                                                            aCCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GAGTTGCTCGCCCGGCA..GGGCGCGGGCAc.gggcggc                     <  2:3557927/101‑6 (MQ=255)
                                                                            aCCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GAGTTGCTCGCCCGGCA..GGGCGCGGGCAc.gggcggc                     <  2:2724240/101‑6 (MQ=255)
                                                                               gCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GAGTTGCTCGCCCGGCA..GGGCGCGGGCACGGGcggcccg                   >  1:4273433/1‑101 (MQ=255)
                                                                               gCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GAGTTGCTCGCCCGGCA..GGGCGCGGGCACGGGcggcccg                   <  2:432383/101‑1 (MQ=255)
                                                                                    gCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GAGTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGa              <  1:1545486/101‑1 (MQ=255)
                                                                                     ccGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GAGTTGCTCGCCCGGCAGGGGGCGG.GGCCG.GGCGGCCCGGCCGAt             >  2:3621401/1‑101 (MQ=255)
                                                                                     ccGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GAGTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGGCCGGCCggt             >  2:144133/1‑99 (MQ=255)
                                                                                     ccGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GAGTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGAt             <  2:1842370/101‑1 (MQ=255)
                                                                                      cgcgGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GAGTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCGGATc            <  2:1266917/101‑1 (MQ=255)
                                                                                       gcgGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GAGTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCt           <  1:3688323/101‑1 (MQ=255)
                                                                                       gcgGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GAGTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCt           <  2:368788/101‑1 (MQ=255)
                                                                                       gcgGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GAGTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCt           <  1:728597/101‑1 (MQ=255)
                                                                                          ggTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GAGTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCg        >  1:2203754/1‑101 (MQ=255)
                                                                                            tCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GAGTTGCTCGCCCGGCA..GGGCGCGGGCACGGGGGGCCCGGCCGGGCTGCggg      >  1:62098/1‑100 (MQ=255)
                                                                                             cTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GAGTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCt     <  1:822589/101‑1 (MQ=255)
                                                                                               gACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GAGTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTgg   <  1:178329/101‑1 (MQ=255)
                                                                                                aCCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GAGTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTggg  >  2:2149075/1‑100 (MQ=255)
                                                                                                     |                                                                                                   
TCGGTGGCGGGGGTCGATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCT.GCCGAGG.GCAG.TGGAA.CCGCGATGG.AA.GGCT.GCC.GAG.TGTTCGCCCGGCA..GGGCGC.GGCAC.GGCGGCCCGGCCGATCTGCGGCTGGA  >  NC_014034/3256064‑3256251

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG