Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,256,175 +C 100% coding (1318/1734 nt) recJ → single‑stranded‑DNA‑specific exonuclease RecJ

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,256,1751.→C100.0% 624.3 162coding (1318/1734 nt)recJsingle‑stranded‑DNA‑specific exonuclease RecJ
Reads supporting (aligned to +/- strand):  new base (79/83):  ref base (0/0):  total (79/83)

Only 100 of 165 total aligned reads displayed.

GGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCT.GCCGAGG.GCAG.TGGAA.CCGCGATGG.AA.GGCT.GCC.GA.G.TGTTCGCCCGGCA..GGGCGC.GGCA.C.GGCGGCCCGGCCGATCTGCGGCTGGACGGGCT  >  NC_014034/3256084‑3256257
                                                                                              |                                                                                              
gggCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAA.c                                                                                         <  1:4167902/101‑1 (MQ=255)
gggCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAA.c                                                                                         <  1:4440700/101‑1 (MQ=255)
gggCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAA.c                                                                                         >  1:2724437/1‑101 (MQ=255)
 ggCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAA.cc                                                                                        <  2:2371062/101‑1 (MQ=255)
  gcgcGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAA.ccc                                                                                       <  2:1585600/101‑2 (MQ=255)
  gcgcGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAA.ccc                                                                                       <  2:4270694/101‑2 (MQ=255)
   ggcggcgGTGCAGCGCGTCGGGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAA.cccg                                                                                      <  2:1121474/100‑3 (MQ=255)
   cgcgGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAA.cccg                                                                                      <  2:1485679/101‑3 (MQ=255)
    gcggcgGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAA.cccgc                                                                                     >  1:706202/1‑98 (MQ=255)
      ggcggTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAA.cccgcga                                                                                   >  1:50996/1‑96 (MQ=255)
      ggcggTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAA.cccgcga                                                                                   >  2:1904133/1‑96 (MQ=255)
       gcggTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGAt                                                                                   <  1:262031/101‑1 (MQ=255)
        cggTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATg                                                                                  <  1:3560877/101‑1 (MQ=255)
         ggTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATgg                                                                                 <  2:1569736/101‑1 (MQ=255)
         ggTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATgg                                                                                 >  2:3674309/1‑101 (MQ=255)
          gTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATggc                                                                                <  2:1811526/101‑2 (MQ=255)
          gTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATggc                                                                                <  2:3691924/101‑2 (MQ=255)
            gCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATggcac                                                                              >  1:1456001/1‑98 (MQ=255)
            gCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATggcac                                                                              <  1:1793719/101‑4 (MQ=255)
             cAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATggcacg                                                                             <  1:1305569/101‑5 (MQ=255)
             cAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATggcacg                                                                             >  1:2305727/1‑97 (MQ=255)
               gcgcGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATggcacggc                                                                           >  1:1909274/1‑95 (MQ=255)
                cgcgTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATggcacggct                                                                          <  1:444872/101‑8 (MQ=255)
                 gcgTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATggcacggctg                                                                         <  1:2880848/101‑9 (MQ=255)
                 gcgTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATggcacggctg                                                                         <  2:2509045/101‑9 (MQ=255)
                  cgTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCGTGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATggcacggctgg                                                                        <  1:2700696/101‑10 (MQ=255)
                  cgTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATggcacggctgg                                                                        <  2:1542725/101‑10 (MQ=255)
                  cgTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATggcacggctgg                                                                        <  1:376228/101‑10 (MQ=255)
                  cgTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATggcacggctgg                                                                        >  1:3550169/1‑92 (MQ=255)
                  cgTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATggcacggctgg                                                                        >  1:1754873/1‑92 (MQ=255)
                   gTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATggcacggctggc                                                                       >  2:2176497/1‑91 (MQ=255)
                          ccGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GA.g.ttg                                                            >  2:792740/1‑99 (MQ=255)
                          ccGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GA.g.ttg                                                            >  2:1977178/1‑99 (MQ=255)
                          ccGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GA.g.ttg                                                            >  2:1027568/1‑99 (MQ=255)
                          ccGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGG.CACGGCTGGCC.GA.g.ttg                                                            <  2:4397529/101‑3 (MQ=255)
                           cGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GA.g.ttgc                                                           >  1:378386/1‑98 (MQ=255)
                            gAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GA.g.ttgct                                                          >  1:3086159/1‑97 (MQ=255)
                            gAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGCCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GA.g.ttgct                                                          >  1:4513077/1‑97 (MQ=255)
                             aGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GA.g.ttgctc                                                         >  2:4315415/1‑96 (MQ=255)
                             aGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGG.CACGGCTGGCC.GA.g.ttgctc                                                         <  2:687556/101‑6 (MQ=255)
                              ggggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GA.g.ttgctcg                                                        <  2:4535661/101‑7 (MQ=255)
                               gggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GA.g.ttgctcgc                                                       >  2:1397078/1‑94 (MQ=255)
                               gggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GA.g.ttgctcgc                                                       >  1:990426/1‑94 (MQ=255)
                               gggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GA.g.ttgctcgc                                                       >  2:4002870/1‑94 (MQ=255)
                                ggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GA.g.ttgctcgcc                                                      >  2:2086287/1‑93 (MQ=255)
                                 gCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGG.CACGGCTGGCC.GA.GTTGCTCGccc                                                      <  1:61463/101‑1 (MQ=255)
                                  cTTTTGCTCAAGGGCGGCGGGCACCGCGTGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCg                                                     <  2:3305212/101‑1 (MQ=255)
                                  cTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GAGG.TGCTCGcccc                                                     >  2:2907383/1‑100 (MQ=255)
                                  cTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCg                                                     <  2:1606311/101‑1 (MQ=255)
                                  cTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGG.CACGGCTGGCC.GA.GTTGCTCGCCCg                                                     <  2:2618084/101‑1 (MQ=255)
                                   ttttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GA.GTTGCTCGccccg                                                    >  2:4563715/1‑99 (MQ=255)
                                   ttttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGG.CACGGCTGGCC.GA.GTTGCTCGCCCgg                                                    <  2:1334312/101‑1 (MQ=255)
                                       gCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..gg                                              <  2:83276/101‑1 (MQ=255)
                                        cTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..ggg                                             >  2:2062915/1‑101 (MQ=255)
                                        cTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGG.CACGGCTGGCC.GA.GTTGCTCGCCCGGCA..ggg                                             <  2:378349/101‑1 (MQ=255)
                                         tCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GA.GTTGCTCCCCCGGCA..GGgc                                            >  1:2418843/1‑101 (MQ=255)
                                          cAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCCGCA..GGgcg                                           >  1:3013521/1‑101 (MQ=255)
                                           aaGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..gggggg                                          >  1:689307/1‑98 (MQ=255)
                                           aaGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGgcgg                                          >  2:2787796/1‑100 (MQ=255)
                                           aaGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGgcgc                                          >  1:2671207/1‑101 (MQ=255)
                                             gggCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGGG.G.TGCTCGCCCGGCA..GGGCGC.gg                                       >  2:4084916/1‑101 (MQ=255)
                                                cggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..ggggggcggga                                     >  1:3241911/1‑93 (MQ=255)
                                                cggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGC.gggca                                    <  2:272399/101‑4 (MQ=255)
                                                 ggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGGGC.ggggac                                   >  1:4143415/1‑97 (MQ=255)
                                                  gcggGCACCGCATGGCCGCGGGTCTGCCCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGC.gggcacg                                  <  1:1472113/101‑6 (MQ=255)
                                                  gcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGC.gggcacg                                  <  2:3549911/101‑6 (MQ=255)
                                                    gggCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.c.ggg                               >  2:2018647/1‑100 (MQ=255)
                                                     ggCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.c.gggc                              >  1:3413846/1‑99 (MQ=255)
                                                     ggCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGAC.GAGG.TGCTCGCCCGGGA..GGGCGC.gggggacggc                               >  2:3057970/1‑93 (MQ=255)
                                                        aCCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.c.gggcggc                           <  2:2724240/101‑6 (MQ=255)
                                                        aCCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.c.gggcggc                           >  1:286604/1‑96 (MQ=255)
                                                        aCCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.c.gggcggc                           <  2:3557927/101‑6 (MQ=255)
                                                           gCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGcggcccg                         <  2:432383/101‑1 (MQ=255)
                                                           gCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGcggcccg                         >  1:4273433/1‑101 (MQ=255)
                                                            cATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGcggcccgg                        <  2:1277652/101‑1 (MQ=255)
                                                             aTGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGcggcccggc                       >  1:3137880/1‑101 (MQ=255)
                                                               ggCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.c.gggggggcccgccg                    >  2:3448740/1‑92 (MQ=255)
                                                                 ccGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCAGGGGGCGG.GGCC.G.GGCGGCCCGGCCGAt                   >  2:3621401/1‑101 (MQ=255)
                                                                 ccGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGCGGGCCGGCCggt                   >  2:144133/1‑99 (MQ=255)
                                                                 ccGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGCGGCCCGGCCGAt                   <  1:681689/101‑1 (MQ=255)
                                                                  cgcgGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGCGGCCCGGCGGATc                  <  2:1266917/101‑1 (MQ=255)
                                                                   gcgGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGCGGCCCGGCCGATCt                 <  1:1977313/101‑1 (MQ=255)
                                                                   gcgGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGCGGCCCGGCCGATCt                 <  1:2176647/101‑1 (MQ=255)
                                                                   gcgGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGCGGCCCGGCCGATCt                 <  1:728597/101‑1 (MQ=255)
                                                                   gcgGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGCGGCCCGGCCGATCt                 <  1:3626761/101‑1 (MQ=255)
                                                                    cgGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGGACC.GGCGGCCCCGCCGGTCTg                >  2:3822876/1‑101 (MQ=255)
                                                                     gggTCTGCCCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGc               <  2:3135420/101‑1 (MQ=255)
                                                                         cTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCC.CGGGGGGCCCCGCCGGTCTGCGGCt           >  2:2158628/1‑101 (MQ=255)
                                                                         cTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCt           <  1:822589/101‑1 (MQ=255)
                                                                          tgcccctggCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTg          <  2:859482/93‑1 (MQ=255)
                                                                           gACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTgg         <  1:178329/101‑1 (MQ=255)
                                                                            aCCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTggg        >  2:2149075/1‑100 (MQ=255)
                                                                             ccctggCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGAc       <  2:3319809/96‑1 (MQ=255)
                                                                              cctggCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACg      <  1:923382/97‑1 (MQ=255)
                                                                               ctggCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GA.GTTGCACGCCCGGCA..GGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCCGGGCgg     >  1:3947938/4‑101 (MQ=255)
                                                                                tggCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACggg    <  2:4143127/99‑1 (MQ=255)
                                                                                 ggCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGCGGCCCCGCCGATCTGCGGCTGGACggg  >  2:1531256/2‑100 (MQ=255)
                                                                                  gCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCt  <  1:3213608/101‑1 (MQ=255)
                                                                                  gCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCt  <  1:2082738/101‑1 (MQ=255)
                                                                                  gCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCt  <  1:1481791/101‑1 (MQ=255)
                                                                                              |                                                                                              
GGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCT.GCCGAGG.GCAG.TGGAA.CCGCGATGG.AA.GGCT.GCC.GA.G.TGTTCGCCCGGCA..GGGCGC.GGCA.C.GGCGGCCCGGCCGATCTGCGGCTGGACGGGCT  >  NC_014034/3256084‑3256257

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG