Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,256,180 +C 100% coding (1323/1734 nt) recJ → single‑stranded‑DNA‑specific exonuclease RecJ

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,256,1801.→C100.0% 578.4 156coding (1323/1734 nt)recJsingle‑stranded‑DNA‑specific exonuclease RecJ
Reads supporting (aligned to +/- strand):  new base (75/80):  ref base (0/1):  total (75/81)

Only 100 of 179 total aligned reads displayed.

GGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCT.GCCGAGG.GCAG.TGGAA.CC.GCGATGG.AA.GGCT.GCC.GA.G.TGTTCGCCCGGCAGGGCGC.GGCA.C.GGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGC  >  NC_014034/3256084‑3256270
                                                                                                    |                                                                                                    
gggCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAA.c                                                                                                     <  1:4181949/101‑1 (MQ=255)
gggCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAA.c                                                                                                     <  1:4440700/101‑1 (MQ=255)
gggCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAA.c                                                                                                     >  2:376191/1‑101 (MQ=255)
gggCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAA.c                                                                                                     >  1:2724437/1‑101 (MQ=255)
gggCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAA.c                                                                                                     >  1:2628637/1‑101 (MQ=255)
  gcgcGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAA.ccc                                                                                                   <  1:3616940/101‑2 (MQ=255)
  gcgcGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAA.ccc                                                                                                   <  2:4270694/101‑2 (MQ=255)
   ggcggcgGTGCAGCGCGTCGGGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAA.cccg                                                                                                  <  2:1121474/100‑3 (MQ=255)
    gcggcgGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAA.cccgc                                                                                                 >  2:2065282/1‑98 (MQ=255)
      ggcggTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAA.cccgcga                                                                                               >  1:50996/1‑96 (MQ=255)
        cggTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATg                                                                                             >  1:687618/1‑101 (MQ=255)
         ggTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATgg                                                                                            >  2:3674309/1‑101 (MQ=255)
          gTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATggc                                                                                           <  2:1811526/101‑2 (MQ=255)
          gTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATggc                                                                                           <  2:3691924/101‑2 (MQ=255)
            gCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATggcac                                                                                         <  1:1793719/101‑4 (MQ=255)
            gCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATggcac                                                                                         >  1:1456001/1‑98 (MQ=255)
             cAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATggcacg                                                                                        >  1:2305727/1‑97 (MQ=255)
             cAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATggcacg                                                                                        <  1:1305569/101‑5 (MQ=255)
               gcgcGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATggcacggc                                                                                      >  1:1909274/1‑95 (MQ=255)
                cgcgTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATggcacggct                                                                                     >  2:1579596/1‑94 (MQ=255)
                 gcgTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATggcacggctg                                                                                    <  2:2509045/101‑9 (MQ=255)
                 gcgTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGAACCTGGCCGAGGCGCAGCTGGAACCC.GCGATggcacggctg                                                                                    >  2:2171284/1‑93 (MQ=255)
                  cgTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCGTGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATggcacggctgg                                                                                   <  1:2700696/101‑10 (MQ=255)
                  cgTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATggcacggctgg                                                                                   <  1:376228/101‑10 (MQ=255)
                  cgTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATggcacggctgg                                                                                   >  1:3550169/1‑92 (MQ=255)
                  cgTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATggcacggctgg                                                                                   <  2:1542725/101‑10 (MQ=255)
                  cgTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATggcacggctgg                                                                                   >  1:1754873/1‑92 (MQ=255)
                   gTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATggcacggctggc                                                                                  >  2:3558680/1‑91 (MQ=255)
                   gTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATggcacggctggc                                                                                  >  1:3179744/1‑91 (MQ=255)
                         gCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.g.tt                                                                        >  1:713032/1‑100 (MQ=255)
                          ccGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.g.ttg                                                                       >  2:1977178/1‑99 (MQ=255)
                            gAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGCCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.g.ttgct                                                                     >  1:4513077/1‑97 (MQ=255)
                             aGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.g.ttgctc                                                                    >  2:4315415/1‑96 (MQ=255)
                               gggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.g.ttgctcgc                                                                  >  2:4002870/1‑94 (MQ=255)
                               gggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.g.ttgctcgc                                                                  >  2:1397078/1‑94 (MQ=255)
                               gggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.g.ttgctcgc                                                                  >  1:990426/1‑94 (MQ=255)
                                ggCTTTTGGTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.g.ttgctcgcc                                                                 <  2:4487207/101‑9 (MQ=255)
                                  cTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCg                                                                <  2:1606311/101‑1 (MQ=255)
                                  cTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGG.CACGGCTGGCC.GA.GTTGCTCGCCCg                                                                <  2:706127/101‑1 (MQ=255)
                                  cTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGG.CACGGCTGGCC.GA.GTTGCTCGCCCg                                                                <  2:2618084/101‑1 (MQ=255)
                                   ttttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGccccg                                                               >  2:4563715/1‑99 (MQ=255)
                                   ttttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCgg                                                               >  1:829929/1‑101 (MQ=255)
                                   ttttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGG.CACGGCTGGCC.GA.GTTGCTCGCCCgg                                                               <  2:1334312/101‑1 (MQ=255)
                                      tGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCAg                                                            <  2:4384814/101‑1 (MQ=255)
                                       gCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCAgg                                                           <  2:83276/101‑1 (MQ=255)
                                        cTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGG.CACGGCTGGCC.GA.GTTGCTCGCCCGGCAggg                                                          <  2:1286207/101‑1 (MQ=255)
                                           caGGGCGGCGGGCACCGCATGGCCGCGGGCCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GATGCGCGCCCCGCAGGgcgg                                                       >  2:1467636/2‑100 (MQ=255)
                                           aaGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCAgggggg                                                       >  1:689307/1‑98 (MQ=255)
                                           aaGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCAGGgcgg                                                       >  2:2787796/1‑100 (MQ=255)
                                             gggCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGGG.G.TGCTCGCCCGGCAGGGCGC.gg                                                    >  2:4084916/1‑101 (MQ=255)
                                             ggacggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCAGGGGGC.gg                                                    >  1:2463054/4‑101 (MQ=255)
                                                cggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCAggggggcggga                                                  >  1:3241911/1‑93 (MQ=255)
                                                cggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCAGGGCGC.gggca                                                 <  2:2628452/101‑4 (MQ=255)
                                                 ggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCAGGGGGC.ggggac                                                >  1:4143415/1‑97 (MQ=255)
                                                 ggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCAGGGCGC.gggcga                                                >  2:519006/1‑97 (MQ=255)
                                                  gcggGCACCGCATGGCCGCGGGTCTGCCCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCAGGGCGC.gggcacg                                               <  1:1472113/101‑6 (MQ=255)
                                                  gcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCAGGGCGC.gggcacg                                               <  2:3549911/101‑6 (MQ=255)
                                                   cggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCCGGGCGC.ggggaagg                                              >  2:3095308/1‑95 (MQ=255)
                                                   cggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCAGGGCGCGGGCA.c.gg                                             <  2:4156024/101‑1 (MQ=255)
                                                    gggCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCAGGGCGCGGGCA.c.ggg                                            <  1:3795079/101‑2 (MQ=255)
                                                    gggCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCAGGGCGCGGGCA.c.ggg                                            >  2:2018647/1‑100 (MQ=255)
                                                     ggCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCAGGGCGCGGGCA.c.gggc                                           >  1:3413846/1‑99 (MQ=255)
                                                     ggCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGAC.GAGG.TGCTCGCCCGGGAGGGCGC.gggggacggc                                            >  2:3057970/1‑93 (MQ=255)
                                                        aCCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCAGGGCGCGGGCA.c.gggcggc                                        >  1:286604/1‑96 (MQ=255)
                                                        aCCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCAGGGCGCGGGCA.c.gggcggc                                        <  2:2724240/101‑6 (MQ=255)
                                                           gCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCAGGGCNCGGGCA.c.gggcgggccg                                     >  2:1548092/1‑93 (MQ=255)
                                                           gCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGcggcccg                                      >  2:1504866/1‑101 (MQ=255)
                                                           gCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGcggcccg                                      >  1:4273433/1‑101 (MQ=255)
                                                             aTGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGcggcccggc                                    >  1:3137880/1‑101 (MQ=255)
                                                             aTGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGcggcccggc                                    <  1:4479024/101‑1 (MQ=255)
                                                               ggCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCAGGGCGCGGGCA.c.gggggggcccgccg                                 >  2:3448740/1‑92 (MQ=255)
                                                                gCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGa                                 <  1:1545486/101‑1 (MQ=255)
                                                                 ccGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCAGGGGGCGGGCA.CGGGGGGGCCGGCCGAt                                >  1:2839348/1‑101 (MQ=255)
                                                                 ccGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGAt                                <  2:1842370/101‑1 (MQ=255)
                                                                  cgcgGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCGGATc                               <  2:1266917/101‑1 (MQ=255)
                                                                   gcgGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCt                              <  2:2093294/101‑1 (MQ=255)
                                                                   gcgGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCt                              <  1:3626761/101‑1 (MQ=255)
                                                                   gcgGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCt                              <  1:1977313/101‑1 (MQ=255)
                                                                    cgGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCAGGGCGCGGGGACC.GGCGGCCCCGCCGGTCTg                             >  2:3822876/1‑101 (MQ=255)
                                                                      ggTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCAGGGGGCGGGCA.CGGGCGGCCCGGCCGATCTGCg                           >  1:2883351/1‑101 (MQ=255)
                                                                      ggTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCg                           <  1:3024207/101‑1 (MQ=255)
                                                                      ggTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCg                           >  1:2203754/1‑101 (MQ=255)
                                                                        tCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGGGGCCCGGCCGGGCTGCggg                         >  1:62098/1‑100 (MQ=255)
                                                                          tgcccctggCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTg                       <  2:859482/93‑1 (MQ=255)
                                                                            aCCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCNGCCGCTCTGCGGCTGGa                     >  2:2235693/1‑101 (MQ=255)
                                                                            aCCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTggg                     >  2:2149075/1‑100 (MQ=255)
                                                                             ccctggCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGAc                    <  2:3319809/96‑1 (MQ=255)
                                                                               ctggCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCAGGGCGCGGGGA.CGGGCGGCCCGGCCGGTCTGCGGCCgggccg                  >  1:931381/4‑97 (MQ=255)
                                                                               ctggCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCAGGGCGCGGGCC.CGGGGGGGCCGGCCGATCTTCGGCCGGGCgg                  >  2:288264/4‑101 (MQ=255)
                                                                               ctggCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACgg                  <  2:3179501/98‑1 (MQ=255)
                                                                                  gCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCt               <  1:2082738/101‑1 (MQ=255)
                                                                                     gaggcgcagctGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCt            <  1:257630/91‑1 (MQ=255)
                                                                                      aggcgcagctGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTg           <  1:1767024/92‑1 (MQ=255)
                                                                                       ggcgcagctGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGa          <  1:400094/93‑1 (MQ=255)
                                                                                        gcgcggctGGAA.CCGGCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGAt         <  2:1046333/94‑1 (MQ=255)
                                                                                        gcgcagctGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGAt         <  1:2149225/94‑1 (MQ=255)
                                                                                           cagctGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGTCCGATCTGCGGCTGGACGGGCTCCTGATGcc      <  1:4141513/97‑1 (MQ=255)
                                                                                           cagctGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGCTCCTGGTGcc      >  1:738524/5‑101 (MQ=255)
                                                                                              ctGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCggg   >  2:3487769/2‑101 (MQ=255)
                                                                                              ctGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCggg   <  1:1070463/100‑1 (MQ=255)
                                                                                                    |                                                                                                    
GGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCT.GCCGAGG.GCAG.TGGAA.CC.GCGATGG.AA.GGCT.GCC.GA.G.TGTTCGCCCGGCAGGGCGC.GGCA.C.GGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGC  >  NC_014034/3256084‑3256270

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG