Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,256,201 +T 100% coding (1344/1734 nt) recJ → single‑stranded‑DNA‑specific exonuclease RecJ

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,256,2011.→T100.0% 518.6 137coding (1344/1734 nt)recJsingle‑stranded‑DNA‑specific exonuclease RecJ
Reads supporting (aligned to +/- strand):  new base (58/79):  ref base (0/0):  total (58/79)

Only 100 of 169 total aligned reads displayed.

GCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCT.GCCGAGG.GCAG.TGGAA.CC.GCGATGG.AA.GGCT.GCCGA.G.TGTTCGCCCGGCA..GGGCGC.GGCAC.GGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGA  >  NC_014034/3256109‑3256293
                                                                                                      |                                                                                                
gCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGG.CACGGCTGGCCGA.g.tt                                                                                                <  1:4376935/101‑2 (MQ=255)
 ccGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGA.g.ttg                                                                                               >  2:792740/1‑99 (MQ=255)
 ccGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGG.CACGGCTGGCCGA.g.ttg                                                                                               <  2:4397529/101‑3 (MQ=255)
  cGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGA.g.ttgc                                                                                              >  1:378386/1‑98 (MQ=255)
   gAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGA.g.ttgct                                                                                             >  2:838573/1‑97 (MQ=255)
   gAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGCCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGA.g.ttgct                                                                                             >  1:4513077/1‑97 (MQ=255)
     ggggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGA.g.ttgctcg                                                                                           <  2:4535661/101‑7 (MQ=255)
     ggggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGA.g.ttgctcg                                                                                           >  1:1838372/1‑95 (MQ=255)
      gggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGA.g.ttgctcgc                                                                                          >  1:990426/1‑94 (MQ=255)
      gggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGA.g.ttgctcgc                                                                                          >  2:1397078/1‑94 (MQ=255)
       ggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGA.g.ttgctcgcc                                                                                         >  2:2086287/1‑93 (MQ=255)
        gCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGG.CACGGCTGGCCGA.GTTGCTCGccc                                                                                         <  1:61463/101‑1 (MQ=255)
         cTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGAGG.TGCTCGcccc                                                                                        >  2:2907383/1‑100 (MQ=255)
          ttttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCgg                                                                                       >  2:871537/1‑101 (MQ=255)
          ttttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGG.CACGGCTGGCCGA.GTTGCTCGCCCgg                                                                                       <  2:1334312/101‑1 (MQ=255)
               cTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGG.CACGGCTGGCCGA.GTTGCTCGCCCGGCA..ggg                                                                                <  2:378349/101‑1 (MQ=255)
                tCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCC..gggg                                                                               >  2:2512960/1‑100 (MQ=255)
                tCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCCCCCGGCA..GGgc                                                                               >  1:2418843/1‑101 (MQ=255)
                  caGGGCGGCGGGCACCGCATGGCCGCGGGCCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGA.GATGCGCGCCCCGCA..GGgcgg                                                                             >  2:1467636/2‑100 (MQ=255)
                  aaGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..gggggg                                                                             >  1:689307/1‑98 (MQ=255)
                   aGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGG.CACGGCTGGCCGA.GTTGCTCGCCCGGCA..GGGcgc.g                                                                           <  2:163518/101‑1 (MQ=255)
                    ggacggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGGGC.gg                                                                          >  1:2463054/4‑101 (MQ=255)
                       cggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGC.gggca                                                                       <  2:2628452/101‑4 (MQ=255)
                       cggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCCGCC..GGGGGC.ggggc                                                                       >  2:4067713/1‑98 (MQ=255)
                        ggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGC.gggcga                                                                      >  2:519006/1‑97 (MQ=255)
                        ggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGC.gggcac                                                                      <  1:4105639/101‑5 (MQ=255)
                         gcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGC.gggcacg                                                                     <  2:3549911/101‑6 (MQ=255)
                           gggCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCAc.ggg                                                                   >  2:2018647/1‑100 (MQ=255)
                           gggCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGC.ggggggggg                                                                   >  1:3320058/1‑94 (MQ=255)
                            ggCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCAc.gggc                                                                  >  1:3413846/1‑99 (MQ=255)
                            ggCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGACGAGG.TGCTCGCCCGGGA..GGGCGC.gggggacggc                                                                  >  2:3057970/1‑93 (MQ=255)
                               aCCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCAc.gggcggc                                                               <  2:2724240/101‑6 (MQ=255)
                               aCCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCAc.gggcggc                                                               <  2:1978713/101‑6 (MQ=255)
                                 cGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGcggccc                                                              <  2:2730771/101‑1 (MQ=255)
                                  gCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCNCGGGCAc.gggcgggccg                                                            >  2:1548092/1‑93 (MQ=255)
                                  gCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGcggcccg                                                             <  1:2703498/101‑1 (MQ=255)
                                  gCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGcggcccg                                                             <  2:432383/101‑1 (MQ=255)
                                  gCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGcggcccg                                                             >  1:4273433/1‑101 (MQ=255)
                                   cATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGcggcccgg                                                            <  2:1277652/101‑1 (MQ=255)
                                      ggCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCAc.gggggggcccgccg                                                        >  2:3448740/1‑92 (MQ=255)
                                       gCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGa                                                        <  1:1545486/101‑1 (MQ=255)
                                        ccGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGGGCGG.GGCCG.GGCGGCCCGGCCGAt                                                       >  2:3621401/1‑101 (MQ=255)
                                        ccGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGGGCGGGCACGGGGGGGCCGGCCGAt                                                       >  1:2839348/1‑101 (MQ=255)
                                        ccGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGAt                                                       <  1:681689/101‑1 (MQ=255)
                                          gcgGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATcc                                                     >  2:1163564/1‑100 (MQ=255)
                                          gcgGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCt                                                     <  1:728597/101‑1 (MQ=255)
                                          gcgGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCt                                                     <  1:2176647/101‑1 (MQ=255)
                                          gcgGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCt                                                     <  1:3626761/101‑1 (MQ=255)
                                            gggTCTGCCCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGc                                                   <  2:3135420/101‑1 (MQ=255)
                                             ggTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGGGCGGGCACGGGCGGCCCGGCCGATCTGCg                                                  >  1:2883351/1‑101 (MQ=255)
                                             ggTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCg                                                  <  1:3024207/101‑1 (MQ=255)
                                                cTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCt                                               <  1:822589/101‑1 (MQ=255)
                                                  gACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTgg                                             <  1:178329/101‑1 (MQ=255)
                                                   aCCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGGTCTGCGGCCggg                                            >  1:3416844/1‑100 (MQ=255)
                                                   aCCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTggg                                            >  2:2149075/1‑100 (MQ=255)
                                                    ccctggCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGAc                                           <  2:3319809/96‑1 (MQ=255)
                                                     cctggCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACg                                          <  1:923382/97‑1 (MQ=255)
                                                      ctggCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCCCGGGGGGGCCGGCCGATCTTCGGCCGGGCgg                                         >  2:288264/4‑101 (MQ=255)
                                                      ctggCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCACGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCCGGGCgg                                         >  1:3947938/4‑101 (MQ=255)
                                                       tggCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACggg                                        <  2:4143127/99‑1 (MQ=255)
                                                        ggCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCCGCCGATCTGCGGCTGGACgggg                                       >  2:1531256/2‑100 (MQ=255)
                                                         gCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCt                                      <  1:1481791/101‑1 (MQ=255)
                                                         gCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCt                                      <  1:3213608/101‑1 (MQ=255)
                                                             aggcgcagctGGAACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTg                                  <  1:1767024/92‑1 (MQ=255)
                                                              ggcgcagctGGAACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGa                                 <  1:400094/93‑1 (MQ=255)
                                                               gcgcggctGGAA.CCGGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGAt                                <  2:1046333/94‑1 (MQ=255)
                                                               gcgcagctGGAACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGAt                                <  1:2149225/94‑1 (MQ=255)
                                                                 gcagctGGAACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGCTCCCGATGc                              >  1:1410900/6‑101 (MQ=255)
                                                                  cagctGGAACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGTCCGATCTGCGGCTGGACGGGCTCCTGATGcc                             <  1:4141513/97‑1 (MQ=255)
                                                                     ctGGAACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCggg                          <  1:1070463/100‑1 (MQ=255)
                                                                      tGGAACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGCTCCTGGTGCCcggg                         >  1:4235307/1‑100 (MQ=255)
                                                                      tGGAACCC.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGgc                         <  1:2787994/101‑1 (MQ=255)
                                                                       ggaaccc.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGGGGGGCTCCTGATGCCggggc                        >  2:4188953/6‑99 (MQ=255)
                                                                       ggaaccc.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGgcg                        >  2:2153118/6‑101 (MQ=255)
                                                                        gaaccc.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGCTCCTGATGCCGGgcgc                       >  2:4408396/5‑101 (MQ=255)
                                                                         aaccc.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGcgcc                      <  2:3265620/98‑1 (MQ=255)
                                                                         aaccc.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGcgcc                      <  1:1130446/98‑1 (MQ=255)
                                                                         aaccc.GCGATGG.CACGGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGcgcc                      >  1:3127101/4‑101 (MQ=255)
                                                                          accc.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGcgccg                     <  2:2203604/99‑1 (MQ=255)
                                                                          accc.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGcgccg                     <  1:3078260/99‑1 (MQ=255)
                                                                          accc.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGcgccg                     <  1:2065422/99‑1 (MQ=255)
                                                                          accc.GCGATGG.CACGGCTGGCCGA.GTTGCTCGCCCGGCA..GGGGGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGGCCCCGGTGCCCGGcgccc                     >  1:3195257/3‑100 (MQ=255)
                                                                          accc.GCGATGG.CACGGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGCTCCCGATGCCGGGggccc                     >  2:3583706/3‑100 (MQ=255)
                                                                            cc.GCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCcgcg                   <  1:838649/101‑1 (MQ=255)
                                                                             c.gcgATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGCTCCTGGTGCCCGGCGCcgcgc                  >  2:2500388/1‑100 (MQ=255)
                                                                                     ggcacggctggCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCcg            <  2:3413592/91‑1 (MQ=255)
                                                                                     ggcacggctggCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCcg            <  2:4273145/91‑1 (MQ=255)
                                                                                       cacggctggCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGGCGATCTGCGGCTGGACGGGGTCCTGCTGCCGGGCGCCGCGACGCCcgcg          >  2:2799062/9‑101 (MQ=255)
                                                                                       cacggctggCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGTGCTCCTGATGCCGGGCGCCGCGACGCCcgcg          <  1:4176276/93‑1 (MQ=255)
                                                                                        acggctggCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGACGCCGGGCGCCGCGACGCCCgcgc         >  2:4660252/8‑101 (MQ=255)
                                                                                           gctggCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGa      <  2:689245/97‑1 (MQ=255)
                                                                                            ctggCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCcgccccgac     >  1:2222241/4‑95 (MQ=255)
                                                                                            ctggCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGAt     >  2:3952199/4‑101 (MQ=255)
                                                                                            ctggCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGAt     <  2:1925628/98‑1 (MQ=255)
                                                                                            ctggCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGAt     <  1:4067999/98‑1 (MQ=255)
                                                                                             tggCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATc    >  1:2658900/3‑101 (MQ=255)
                                                                                             tggCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATc    <  2:829853/99‑1 (MQ=255)
                                                                                             tggCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCCGATc    >  2:3257902/3‑101 (MQ=255)
                                                                                             tggCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGAGCCTGATGCCCGGACACGCGGAGCCCGCGCGGAtg    >  2:3958481/3‑100 (MQ=255)
                                                                                               gCCGA.GTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGa  <  1:4662254/101‑1 (MQ=255)
                                                                                                      |                                                                                                
GCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCT.GCCGAGG.GCAG.TGGAA.CC.GCGATGG.AA.GGCT.GCCGA.G.TGTTCGCCCGGCA..GGGCGC.GGCAC.GGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGA  >  NC_014034/3256109‑3256293

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG