Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,256,220 +G 100% coding (1363/1734 nt) recJ → single‑stranded‑DNA‑specific exonuclease RecJ

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,256,2201.→G100.0% 510.6 136coding (1363/1734 nt)recJsingle‑stranded‑DNA‑specific exonuclease RecJ
Reads supporting (aligned to +/- strand):  new base (50/85):  ref base (0/1):  total (50/86)

Only 100 of 173 total aligned reads displayed.

AAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCT.GCCGAGG.GCAG.TGGAA.CCGCGATGG.AA.GGCT.GCCGAG.TGTTCGCCCGGCAGGGCGC.GGCA.C.GGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGACCGCGCCGGCCC  >  NC_014034/3256127‑3256314
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aaGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGgcgc                                                                                                   >  1:2671207/1‑101 (MQ=255)
     cggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCGC.gggca                                                                                             <  2:272399/101‑4 (MQ=255)
     cggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCCGCCGGGGGC.ggggc                                                                                             >  2:4067713/1‑98 (MQ=255)
      ggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGGGC.ggggac                                                                                            >  1:4143415/1‑97 (MQ=255)
       gcggGCACCGCATGGCCGCGGGTCTGCCCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCGC.gggcacg                                                                                           <  1:1472113/101‑6 (MQ=255)
       gcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCGC.gggcacg                                                                                           <  2:3549911/101‑6 (MQ=255)
        cggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCCGGGCGC.ggggaagg                                                                                          >  2:3095308/1‑95 (MQ=255)
        cggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.c.gg                                                                                         <  2:4156024/101‑1 (MQ=255)
         gggCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.c.ggg                                                                                        >  2:2018647/1‑100 (MQ=255)
         gggCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.c.ggg                                                                                        <  1:3795079/101‑2 (MQ=255)
         gggCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCGC.ggggggggg                                                                                         >  1:3320058/1‑94 (MQ=255)
          ggCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.c.gggc                                                                                       >  1:3413846/1‑99 (MQ=255)
               cGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGcggccc                                                                                   <  2:2730771/101‑1 (MQ=255)
                gCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGcggcccg                                                                                  <  1:2703498/101‑1 (MQ=255)
                gCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGcggcccg                                                                                  >  1:4273433/1‑101 (MQ=255)
                gCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGcggcccg                                                                                  <  2:432383/101‑1 (MQ=255)
                gCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGcggcccg                                                                                  >  2:1504866/1‑101 (MQ=255)
                 cATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGcggcccgg                                                                                 <  2:1277652/101‑1 (MQ=255)
                 cATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGcggcccgg                                                                                 <  1:505880/101‑1 (MQ=255)
                  aTGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGcggcccggc                                                                                <  1:4479024/101‑1 (MQ=255)
                  aTGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGcggcccggc                                                                                >  1:3137880/1‑101 (MQ=255)
                    ggCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.c.gggggggcccgccg                                                                             >  2:3448740/1‑92 (MQ=255)
                     gCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGa                                                                             <  1:1545486/101‑1 (MQ=255)
                      ccGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGGGCGGGCA.CGGGGGGGCCGGCCGAt                                                                            >  1:2839348/1‑101 (MQ=255)
                        gcgGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATcc                                                                          >  2:1163564/1‑100 (MQ=255)
                        gcgGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCt                                                                          <  1:3688323/101‑1 (MQ=255)
                        gcgGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCt                                                                          <  1:3626761/101‑1 (MQ=255)
                        gcgGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCt                                                                          <  1:728597/101‑1 (MQ=255)
                        gcgGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCt                                                                          <  2:368788/101‑1 (MQ=255)
                        gcgGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCt                                                                          <  1:2176647/101‑1 (MQ=255)
                         cgGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCGCGGGGACC.GGCGGCCCCGCCGGTCTg                                                                         >  2:3822876/1‑101 (MQ=255)
                          gggTCTGCCCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGc                                                                        <  2:3135420/101‑1 (MQ=255)
                           ggTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGGGCGGGCA.CGGGCGGCCCGGCCGATCTGCg                                                                       >  1:2883351/1‑101 (MQ=255)
                           ggTCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCg                                                                       >  1:2203754/1‑101 (MQ=255)
                             tCTGACCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGGGGCCCGGCCGGGCTGCggg                                                                     >  1:62098/1‑100 (MQ=255)
                               tgcccctggCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTg                                                                   <  2:859482/93‑1 (MQ=255)
                                 aCCCTGGCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCNGCCGCTCTGCGGCTGGa                                                                 >  2:2235693/1‑101 (MQ=255)
                                  ccctggCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGAc                                                                <  2:3319809/96‑1 (MQ=255)
                                   cctggCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACg                                                               <  1:923382/97‑1 (MQ=255)
                                    ctggCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCGCGGGGA.CGGGCGGCCCGGCCGGTCTGCGGCCgggccg                                                              >  1:931381/4‑97 (MQ=255)
                                    ctggCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCGCGGGCC.CGGGGGGGCCGGCCGATCTTCGGCCGGGCgg                                                              >  2:288264/4‑101 (MQ=255)
                                    ctggCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCACGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCCGGGCgg                                                              >  1:3947938/4‑101 (MQ=255)
                                      ggCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCCGCCGATCTGCGGCTGGACgggg                                                            >  2:1531256/2‑100 (MQ=255)
                                       gCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCt                                                           <  1:1481791/101‑1 (MQ=255)
                                       gCCGAGGCGCAGCTGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCt                                                           <  1:3213608/101‑1 (MQ=255)
                                            ggcgcagctGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGa                                                      <  1:400094/93‑1 (MQ=255)
                                                cagctGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGTCCGATCTGCGGCTGGACGGGCTCCTGATGcc                                                  <  1:4141513/97‑1 (MQ=255)
                                                cagctGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGCTCCTGGTGcc                                                  >  1:738524/5‑101 (MQ=255)
                                                   ctGGAACCCGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCggg                                               <  1:1070463/100‑1 (MQ=255)
                                                     ggaacccGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGGGGGGCTCCTGATGCCggggc                                             >  2:4188953/6‑99 (MQ=255)
                                                     ggaacccGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGgcg                                             >  2:2153118/6‑101 (MQ=255)
                                                      gaacccGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGGGCCTGGTGCCGGgcgc                                            >  1:3497802/5‑101 (MQ=255)
                                                       aacccGCGATGGCAC.GGCTGGCCGAGTTGCTCGCTCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGcgcc                                           <  1:4003155/98‑1 (MQ=255)
                                                       aacccGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGcgcc                                           <  1:1130446/98‑1 (MQ=255)
                                                       aacccGCGATGG.CACGGCTGGCCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGCCGGGGTCCCTATGCCGGGcgcc                                           >  1:1775183/4‑101 (MQ=255)
                                                        acccGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGcgccg                                          <  1:3078260/99‑1 (MQ=255)
                                                        acccGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGcgccg                                          <  1:2065422/99‑1 (MQ=255)
                                                        acccGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGcgccg                                          <  2:2203604/99‑1 (MQ=255)
                                                        acccGCGATGG.CACGGCTGGCCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGCTCCCGATGCCGGGggccc                                          >  2:3583706/3‑100 (MQ=255)
                                                         cccGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGcgccgc                                         <  1:1355033/100‑1 (MQ=255)
                                                         cccGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGcgccgc                                         <  1:1579700/100‑1 (MQ=255)
                                                          ccGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCcgcg                                        <  1:838649/101‑1 (MQ=255)
                                                           cgcgATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGCTCCTGGTGCCCGGCGCcgcgc                                       >  2:2500388/1‑100 (MQ=255)
                                                                  ggcacggctggCCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCcg                                 <  2:3413592/91‑1 (MQ=255)
                                                                    cacggctggCCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGGCGATCTGCGGCTGGACGGGGTCCTGCTGCCGGGCGCCGCGACGCCcgcg                               >  2:2799062/9‑101 (MQ=255)
                                                                    cacggctggCCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCcgcg                               <  1:4085202/93‑1 (MQ=255)
                                                                     acggctggCCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGACGCCGGGCGCCGCGACGCCCgcgc                              >  2:4660252/8‑101 (MQ=255)
                                                                       ggctggCCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGGCGATCTGCGGCTGGACGGGCTCCTGATTGCGGGCGCCGCGGCGCCCGCGCTg                            >  1:2763766/6‑101 (MQ=255)
                                                                        gctggCCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGa                           <  2:689245/97‑1 (MQ=255)
                                                                         ctggCCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGAt                          >  2:3952199/4‑101 (MQ=255)
                                                                         ctggCCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGAt                          <  1:4189241/98‑1 (MQ=255)
                                                                         ctggCCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGAt                          <  1:4330050/98‑1 (MQ=255)
                                                                         ctggCCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGAt                          <  2:1925628/98‑1 (MQ=255)
                                                                          tggCCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATc                         <  2:829853/99‑1 (MQ=255)
                                                                          tggCCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCCGATc                         >  2:3257902/3‑101 (MQ=255)
                                                                           ggCCGAGTTGCTCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGCTCCTGATGCCGGGCGCCGCCACCCCCGCGCTGCTCg                        >  2:3019898/2‑101 (MQ=255)
                                                                              cgagttgctCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAgg                     >  1:169737/9‑101 (MQ=255)
                                                                               gagttgctCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGa                    <  2:4086080/94‑1 (MQ=255)
                                                                               gagttgctCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGa                    >  1:3008684/8‑101 (MQ=255)
                                                                               gagttgctCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGa                    <  2:2692245/94‑1 (MQ=255)
                                                                               gagttgctCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCCGATCGAGGa                    >  1:2019102/8‑101 (MQ=255)
                                                                                  ttgctCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGAtct                 <  2:1842272/97‑1 (MQ=255)
                                                                                  ttgctCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGAtct                 <  2:2120688/97‑1 (MQ=255)
                                                                                  ttgctCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGAtct                 >  2:2086371/5‑101 (MQ=255)
                                                                                  tggctCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGAtct                 <  2:4015949/97‑1 (MQ=255)
                                                                                  tggcccGCCCGGCAGGCCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGAtct                 <  2:1655803/96‑1 (MQ=255)
                                                                                   tgctCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGAGGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGAAtctt                >  1:2549420/4‑100 (MQ=255)
                                                                                   tgctCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGAtctc                >  1:2644790/4‑101 (MQ=255)
                                                                                    gctCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCg               <  2:915981/99‑1 (MQ=255)
                                                                                    gctCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCg               <  1:1949344/99‑1 (MQ=255)
                                                                                    gctCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCg               <  1:2120393/99‑1 (MQ=255)
                                                                                    gctCGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCg               <  2:1838246/99‑1 (MQ=255)
                                                                                      tCGCCCGGCAGGGCGCGGGCA.CGGGAGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGGGCCGCGGCGCCCGGGCTGATCGAGGATCTCgcc             >  1:2268193/1‑99 (MQ=255)
                                                                                       cGCCCGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCCAGGATCTCGAcc            <  1:2533150/101‑1 (MQ=255)
                                                                                         cccGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGGCGGCACGCCCGCGGCGATCGAGGGTCTCGACcgc          >  2:156949/1‑101 (MQ=255)
                                                                                         cccGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGACcgc          <  2:2872337/101‑1 (MQ=255)
                                                                                          ccGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGACcgcg         >  2:2587240/1‑101 (MQ=255)
                                                                                           cGGCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGACCgcgc        >  2:4255739/1‑101 (MQ=255)
                                                                                            ggCAGGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGACCGCGcc       >  1:1638872/1‑101 (MQ=255)
                                                                                                gggCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGACCGCGCCGGcc   <  1:2459697/101‑1 (MQ=255)
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AAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCT.GCCGAGG.GCAG.TGGAA.CCGCGATGG.AA.GGCT.GCCGAG.TGTTCGCCCGGCAGGGCGC.GGCA.C.GGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGACCGCGCCGGCCC  >  NC_014034/3256127‑3256314

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG