Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,256,225 +G 100% coding (1368/1734 nt) recJ → single‑stranded‑DNA‑specific exonuclease RecJ

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,256,2251.→G100.0% 495.6 133coding (1368/1734 nt)recJsingle‑stranded‑DNA‑specific exonuclease RecJ
Reads supporting (aligned to +/- strand):  new base (46/86):  ref base (0/1):  total (46/87)

Only 100 of 167 total aligned reads displayed.

CGGGCACCGCATGGCCGCGGGTCTGACCCT.GCCGAGG.GCAG.TGGAA.CC.GCGATGG.AA.GGCT.GCCGAG.TGTTCGCCCGGCA..GGGCGC.GGCAC.GGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGACCGCGCCGGCCCCTATGG  >  NC_014034/3256135‑3256320
                                                                                                       |                                                                                               
cggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCGCGGGCAc.gg                                                                                               <  2:4156024/101‑1 (MQ=255)
 gggCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCGCGGGCAc.ggg                                                                                              <  1:3795079/101‑2 (MQ=255)
 gggCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCGCGGGCAc.ggg                                                                                              >  2:2018647/1‑100 (MQ=255)
     aCCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCGCGGGCAc.gggcggc                                                                                          <  2:3557927/101‑6 (MQ=255)
     aCCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCGCGGGCAc.gggcggc                                                                                          <  2:1978713/101‑6 (MQ=255)
     aCCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCGCGGGCAc.gggcggc                                                                                          >  1:286604/1‑96 (MQ=255)
     aCCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCGCGGGCAc.gggcggc                                                                                          <  2:2724240/101‑6 (MQ=255)
       cGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCGCGGGCACGGGcggccc                                                                                         <  2:2730771/101‑1 (MQ=255)
        gCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCGCGGGCACGGGcggcccg                                                                                        <  2:432383/101‑1 (MQ=255)
        gCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCGCGGGCACGGGcggcccg                                                                                        >  1:4273433/1‑101 (MQ=255)
        gCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCGCGGGCACGGGcggcccg                                                                                        >  2:1504866/1‑101 (MQ=255)
         cATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCGCGGGCACGGGcggcccgg                                                                                       <  1:505880/101‑1 (MQ=255)
         cATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCGCGGGCACGGGcggcccgg                                                                                       <  2:1277652/101‑1 (MQ=255)
          aTGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCGCGGGCACGGGcggcccggc                                                                                      <  1:4479024/101‑1 (MQ=255)
          aTGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCGCGGGCACGGGcggcccggc                                                                                      >  1:3137880/1‑101 (MQ=255)
            ggCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCGCGGGCAc.gggggggcccgccg                                                                                   >  2:3448740/1‑92 (MQ=255)
             gCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGa                                                                                   <  1:1545486/101‑1 (MQ=255)
              ccGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCAGGGGGCGG.GGCCG.GGCGGCCCGGCCGAt                                                                                  >  2:3621401/1‑101 (MQ=255)
              ccGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGGGCGGGCACGGGGGGGCCGGCCGAt                                                                                  >  1:2839348/1‑101 (MQ=255)
              ccGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGAt                                                                                  <  2:1842370/101‑1 (MQ=255)
                gcgGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATcc                                                                                >  2:1163564/1‑100 (MQ=255)
                gcgGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCt                                                                                <  2:368788/101‑1 (MQ=255)
                gcgGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCt                                                                                <  1:2176647/101‑1 (MQ=255)
                gcgGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCt                                                                                <  1:1977313/101‑1 (MQ=255)
                gcgGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCt                                                                                <  1:728597/101‑1 (MQ=255)
                gcgGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCt                                                                                <  2:2093294/101‑1 (MQ=255)
                   ggTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGGGCGGGCACGGGCGGCCCGGCCGATCTGCg                                                                             >  1:2883351/1‑101 (MQ=255)
                   ggTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCg                                                                             <  1:3024207/101‑1 (MQ=255)
                   ggTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCg                                                                             >  1:2203754/1‑101 (MQ=255)
                     tCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCGCGGGCACGGGGGGCCCGGCCGGGCTGCggg                                                                           >  1:62098/1‑100 (MQ=255)
                      cTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCt                                                                          <  1:822589/101‑1 (MQ=255)
                        gACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTgg                                                                        <  1:178329/101‑1 (MQ=255)
                         aCCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGGTCTGCGGCCggg                                                                       >  1:3416844/1‑100 (MQ=255)
                         aCCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTggg                                                                       >  2:2149075/1‑100 (MQ=255)
                          ccctggCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGAc                                                                      <  2:3319809/96‑1 (MQ=255)
                           cctggCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACg                                                                     <  1:923382/97‑1 (MQ=255)
                            ctggCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCGCGGGCCCGGGGGGGCCGGCCGATCTTCGGCCGGGCgg                                                                    >  2:288264/4‑101 (MQ=255)
                            ctggCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACgg                                                                    <  2:3179501/98‑1 (MQ=255)
                            ctggCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGAGTTGCACGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCCGGGCgg                                                                    >  1:3947938/4‑101 (MQ=255)
                              ggCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCCGCCGATCTGCGGCTGGACgggg                                                                  >  2:1531256/2‑100 (MQ=255)
                               gCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCt                                                                 <  1:1481791/101‑1 (MQ=255)
                               gCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCt                                                                 <  1:3213608/101‑1 (MQ=255)
                               gCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCt                                                                 <  1:2082738/101‑1 (MQ=255)
                                  gaggcgcagctGGAACCC.GCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCt                                                              <  1:257630/91‑1 (MQ=255)
                                    ggcgcagctGGAACCC.GCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGa                                                            <  1:400094/93‑1 (MQ=255)
                                     gcgcggctGGAA.CCGGCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGAt                                                           <  2:1046333/94‑1 (MQ=255)
                                     gcgcagctGGAACCC.GCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGAt                                                           <  1:2149225/94‑1 (MQ=255)
                                       gcagctGGAACCC.GCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGCTCCCGATGc                                                         >  1:1410900/6‑101 (MQ=255)
                                           ctGGAACCC.GCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCggg                                                     <  1:1070463/100‑1 (MQ=255)
                                             ggaaccc.GCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGgcg                                                   >  2:2153118/6‑101 (MQ=255)
                                              gaaccc.GCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGGGCCTGGTGCCGGgcgc                                                  >  1:3497802/5‑101 (MQ=255)
                                              gaaccc.GCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGCTCCTGATGCCGGgcgc                                                  >  2:4408396/5‑101 (MQ=255)
                                              gaaccc.GCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGgcgc                                                  <  2:988188/97‑1 (MQ=255)
                                               aaccc.GCGATGGCAC.GGCTGGCCGAGTTGCTCGCTCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGcgcc                                                 <  1:4003155/98‑1 (MQ=255)
                                               aaccc.GCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGcgcc                                                 <  1:1130446/98‑1 (MQ=255)
                                               aaccc.GCGATGG.CACGGCTGGCCGAGTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGCCGGGGTCCCTATGCCGGGcgcc                                                 >  1:1775183/4‑101 (MQ=255)
                                                accc.GCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGcgccg                                                <  1:2065422/99‑1 (MQ=255)
                                                accc.GCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGcgccg                                                <  1:3078260/99‑1 (MQ=255)
                                                accc.GCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGcgccg                                                <  2:2203604/99‑1 (MQ=255)
                                                 ccc.GCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGcgccgc                                               <  1:1355033/100‑1 (MQ=255)
                                                 ccc.GCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGcgccgc                                               <  1:1579700/100‑1 (MQ=255)
                                                  cc.GCGATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCcgcg                                              <  1:838649/101‑1 (MQ=255)
                                                   c.gcgATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGGTCCCGGTGCCGGGggccgggg                                             >  1:3322884/1‑98 (MQ=255)
                                                   c.gcgATGGCAC.GGCTGGCCGAGTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGCTCCTGGTGCCCGGCGCcgcgc                                             >  2:2500388/1‑100 (MQ=255)
                                                           ggcacggctggCCGAGTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCcg                                       <  2:3413592/91‑1 (MQ=255)
                                                             cacggctggCCGAGTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGGCGATCTGCGGCTGGACGGGGTCCTGCTGCCGGGCGCCGCGACGCCcgcg                                     >  2:2799062/9‑101 (MQ=255)
                                                             cacggctggCCGAGTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGTGCTCCTGATGCCGGGCGCCGCGACGCCcgcg                                     <  1:4176276/93‑1 (MQ=255)
                                                             cacggctggCCGAGTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCcgcg                                     <  1:4085202/93‑1 (MQ=255)
                                                                ggctggCCGAGTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGGCGATCTGCGGCTGGACGGGCTCCTGATTGCGGGCGCCGCGGCGCCCGCGCTg                                  >  1:2763766/6‑101 (MQ=255)
                                                                 gctggCCGAGTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGa                                 <  2:689245/97‑1 (MQ=255)
                                                                  ctggCCGAGTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGAt                                <  1:4189241/98‑1 (MQ=255)
                                                                  ctggCCGAGTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGAt                                <  1:4330050/98‑1 (MQ=255)
                                                                   tggCCGAGTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATc                               <  2:829853/99‑1 (MQ=255)
                                                                   tggCCGAGTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATc                               >  1:2658900/3‑101 (MQ=255)
                                                                   tggCCGAGTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCCGATc                               >  2:3257902/3‑101 (MQ=255)
                                                                   tggCCGAGTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGAGCCTGATGCCCGGACACGCGGAGCCCGCGCGGAtg                               >  2:3958481/3‑100 (MQ=255)
                                                                    ggCCGAGTTGCTCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGCTCCTGATGCCGGGCGCCGCCACCCCCGCGCTGCTCg                              >  2:3019898/2‑101 (MQ=255)
                                                                      ccgagttgctCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAg                            <  2:3908985/92‑1 (MQ=255)
                                                                       cgagttgctCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGCTCCTGATGCCGGGGGCCGCGACGCCCGCGCTGATCCAgg                           >  1:3740843/9‑101 (MQ=255)
                                                                       cgagttgctCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAgg                           >  1:169737/9‑101 (MQ=255)
                                                                        gagttgctCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGa                          >  1:3544758/8‑101 (MQ=255)
                                                                          gttgctCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGAtc                        >  2:4184828/6‑101 (MQ=255)
                                                                           tggctCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGAtct                       <  2:4015949/97‑1 (MQ=255)
                                                                             gctCGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCg                     <  2:1838246/99‑1 (MQ=255)
                                                                               tCGCCCGGCA..GGGCGCGGGCACGGGAGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGGGCCGCGGCGCCCGGGCTGATCGAGGATCTCgcc                   >  1:2268193/1‑99 (MQ=255)
                                                                                cGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGAcc                  <  2:886702/101‑1 (MQ=255)
                                                                                cGCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCCAGGATCTCGAcc                  <  1:2533150/101‑1 (MQ=255)
                                                                                 gCCCGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGACcg                 <  2:1754754/101‑1 (MQ=255)
                                                                                  cccGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGGCGGCACGCCCGCGGCGATCGAGGGTCTCGACcgc                >  2:156949/1‑101 (MQ=255)
                                                                                  cccGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGACcgc                <  2:2872337/101‑1 (MQ=255)
                                                                                   ccGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGACcgcg               >  2:2587240/1‑101 (MQ=255)
                                                                                   ccGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGACcgcg               >  2:244569/1‑101 (MQ=255)
                                                                                    cGGCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGACCgcgc              >  2:4255739/1‑101 (MQ=255)
                                                                                     ggCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGACCGCGcc             >  1:2677002/1‑101 (MQ=255)
                                                                                      gCA..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGACCGCGCCg            <  1:1620684/101‑1 (MQ=255)
                                                                                        a..GGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGACCGCGCCGGc          >  2:832730/1‑101 (MQ=255)
                                                                                           gggCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGACCGCGCCGGcc         <  1:2459697/101‑1 (MQ=255)
                                                                                            ggCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGACCGCGCCGGccc        <  1:612023/101‑1 (MQ=255)
                                                                                               gcgggCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGACCGCGCCGGCCCCTa     >  1:3651358/4‑101 (MQ=255)
                                                                                                 gggCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGACCGCGCCGGCCCCTATg   >  2:4105905/2‑101 (MQ=255)
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CGGGCACCGCATGGCCGCGGGTCTGACCCT.GCCGAGG.GCAG.TGGAA.CC.GCGATGG.AA.GGCT.GCCGAG.TGTTCGCCCGGCA..GGGCGC.GGCAC.GGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGACCGCGCCGGCCCCTATGG  >  NC_014034/3256135‑3256320

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG