Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,258,963 +C 100% coding (778/1971 nt) efeU → ferrous iron permease EfeU

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,258,9631.→C100.0% 66.6 207coding (778/1971 nt)efeUferrous iron permease EfeU
Reads supporting (aligned to +/- strand):  new base (108/77):  ref base (0/22):  total (108/99)
Fisher's exact test strand distribution p-value = 2.29e-08
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.10e-02

Only 100 of 237 total aligned reads displayed.

AATGCCTCGGTCGCGGGCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGG.CCGTG.A.CCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAGCGCCGTCCGGGCGCCTCCTT  >  NC_014034/3258864‑3259058
                                                                                                      |                                                                                               
 aTGCCTCGGTCGCGGGCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGG.CCGTG.A.cc                                                                                               <  2:1513416/101‑1 (MQ=255)
   gCCTCGGTCGCGGGCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGG.CCGTG.A.ccct                                                                                             <  2:2632607/101‑3 (MQ=255)
    ccTCGGTCGCGGGCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGG.CCGTG.A.ccctg                                                                                            <  2:1452880/101‑4 (MQ=255)
    ccTCGGTCGCGGGCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGG.CCGTG.A.ccctg                                                                                            >  2:4627697/1‑98 (MQ=255)
     cTCGGTCGCGGGCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGG.CCGTG.A.ccctgc                                                                                           >  1:806553/1‑97 (MQ=255)
      tCGGTCGCGGGCACCGTGCTTTCGGCGCTCTTTGGCTATCACAACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGG.CCGTG.A.ccctgcc                                                                                          >  2:2380915/1‑96 (MQ=255)
        ggTCGCGGGCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGG.CCGTG.ACCCTGCCGc                                                                                         >  2:1660058/1‑101 (MQ=255)
        ggTCGCGGGCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGG.CCGTG.ACCCTGCCGc                                                                                         >  1:3614306/1‑101 (MQ=255)
         gTCGCGGGCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGG.CCGTG.ACCCTGCCGct                                                                                        >  1:4646725/1‑101 (MQ=255)
          tCGCGGGCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGG.CCGTG.ACCCTGCCGctc                                                                                       >  1:1753386/1‑101 (MQ=255)
          tCGCGGGCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGG.CCGTG.ACCCTGCCGctc                                                                                       <  1:3522395/101‑1 (MQ=255)
           cgcgGGCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGG.CCGTG.ACCCTGCCGctct                                                                                      <  1:3865064/101‑1 (MQ=255)
           cgcgGGCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGG.CCGTG.ACCCTGCCGctct                                                                                      <  1:2861822/101‑1 (MQ=255)
           cgcgGGCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGG.CCGTG.ACCCTGCCGctct                                                                                      <  1:1384021/101‑1 (MQ=255)
            gcgGGCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGG.CCGTG.ACCCTGCCGCTCtt                                                                                     <  2:3340705/101‑1 (MQ=255)
               ggCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGG.CCGTG.ACCCTGCCGCTCTTCCt                                                                                  >  2:2925520/1‑101 (MQ=255)
                gCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGG.CCGTG.ACCCTGCCGCTCTTCCTg                                                                                 <  1:1183790/101‑1 (MQ=255)
                gCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGG.CCGTG.ACCCTGCCGCTCTTCCTg                                                                                 >  1:4101146/1‑101 (MQ=255)
                gCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGG.CCGTG.ACCCTGCCGCTCTTCCTg                                                                                 <  2:378266/101‑1 (MQ=255)
                 cACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGG.CCGTG.ACCCTGCCGCTCTTCCTgc                                                                                >  2:1173754/1‑101 (MQ=255)
                 cACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGG.CCGTG.ACCCTGCCGCTCTTCCTgc                                                                                <  2:3016395/101‑1 (MQ=255)
                    cGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGG.CCGTG.ACCCTGCCGCTCTTCCTGCGcc                                                                             >  2:1863885/1‑101 (MQ=255)
                    cGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGG.CCGTG.ACCCTGCCGCTCTTCCTGCGcc                                                                             >  1:3016168/1‑101 (MQ=255)
                      tGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGG.CCGTG.ACCCTGCCGCTCTTCCTGCGcccc                                                                           >  1:1111779/1‑101 (MQ=255)
                      tGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGG.CCGTG.ACCCTGCCGCTCTTCCTGCGcccc                                                                           <  1:1905647/101‑1 (MQ=255)
                         tttCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGG.CCGTG.ACCCTGCCGCTCTTCCTGCGCCCcgcg                                                                        <  2:3695167/101‑1 (MQ=255)
                         tttCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGG.CCGTG.ACCCTGCCGCTCTTCCTGCGCCCcgcg                                                                        >  1:2504907/1‑101 (MQ=255)
                            cGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGG.CCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGcgccc                                                                     <  1:705127/101‑1 (MQ=255)
                            cGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGG.CCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGcgccc                                                                     <  1:1314845/101‑1 (MQ=255)
                             ggCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGG.CCGTG.ACCCTGCCGCTCTTACTGCGCCCCGcgcccg                                                                    >  1:1598971/1‑101 (MQ=255)
                               cgcTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGG.CCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGcgcccgcc                                                                  <  2:762144/101‑1 (MQ=255)
                                gcTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCATCTGGGCCGCGTTCCTGG.CCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCgcccgccc                                                                 <  2:3884514/101‑1 (MQ=255)
                                  tctTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGG.CCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGccccc                                                               <  1:4129986/101‑1 (MQ=255)
                                    tttGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGG.CCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCt                                                             >  1:589602/1‑101 (MQ=255)
                                    tttGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGG.CCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCCCCCGCCCCCCt                                                             >  2:1606436/1‑101 (MQ=255)
                                       ggCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGG.CCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTcc                                                          >  1:765877/1‑101 (MQ=255)
                                       ggCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGG.CCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTcc                                                          <  2:1548166/101‑1 (MQ=255)
                                       ggCTATCACGACAGCCCGACCCAGGGCGAGCTCGCCCTCTGGGCCGCGTTCCTGG.CCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTcc                                                          >  2:3704784/1‑101 (MQ=255)
                                        gCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGG.CCGTGCC.CCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCg                                                         <  2:1800822/101‑1 (MQ=255)
                                         cTATCCCGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGG.CCGTGCC.CCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGc                                                        <  2:3993350/101‑1 (MQ=255)
                                         cTATCACGACAGCTCGACCCAGGGCGAGGTCGCTCTCTGGGCCGCGTTCCTGG.CCGTGCC.CCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGc                                                        <  1:4011641/101‑1 (MQ=255)
                                         cTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGG.CCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGc                                                        <  1:3292376/101‑1 (MQ=255)
                                           aTCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGG.CCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCaa                                                      <  2:1753267/101‑1 (MQ=255)
                                           aTCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGG.CCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCaa                                                      <  1:3095361/101‑1 (MQ=255)
                                            tCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGG.CCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCaaa                                                     >  1:4388208/1‑101 (MQ=255)
                                            tCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGG.CCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCaaa                                                     >  1:2919693/1‑101 (MQ=255)
                                            tCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGG.CCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCaaa                                                     <  1:1092105/101‑1 (MQ=255)
                                             cACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGG.CCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCaaaa                                                    >  1:4490390/1‑101 (MQ=255)
                                             cACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGG.CCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCaaaa                                                    <  1:4297191/101‑1 (MQ=255)
                                             cACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGG.CCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCaaaa                                                    <  2:4256518/101‑1 (MQ=255)
                                             cACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGG.CCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCaaaa                                                    >  2:488633/1‑101 (MQ=255)
                                              aCGACAGCCCGACCCAGGGCGGGGTCGCCCTCTGGGCCGCGTTCCTGG.CCGTGCC.CCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAg                                                   <  2:3100155/101‑1 (MQ=255)
                                               cGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGG.CCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAgg                                                  <  1:4372076/101‑1 (MQ=255)
                                                gACAGCCCGACCCAGGTCGAGGTCGCCCTCTGGGCCCCGTTCCTGG.CCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGc                                                 <  2:2084656/101‑1 (MQ=255)
                                                gACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGG.CCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGc                                                 <  1:3827343/101‑1 (MQ=255)
                                                gACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGG.CCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGc                                                 >  2:2682991/1‑101 (MQ=255)
                                                  cAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGG.CCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCt                                               >  2:4050926/1‑101 (MQ=255)
                                                  cAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGG.CCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCCTCCGCAAAAGGCCt                                               >  2:1698693/1‑101 (MQ=255)
                                                   aGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGG.CCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTg                                              >  1:188165/1‑101 (MQ=255)
                                                   aGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGG.CCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTg                                              >  1:4226547/1‑101 (MQ=255)
                                                   aGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGG.CCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTg                                              <  2:4213646/101‑1 (MQ=255)
                                                   aGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGG.CCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTg                                              >  1:3061148/1‑101 (MQ=255)
                                                   aGCCCGACCCAGGGCGAGGGCGCCCTCTGGGCCGCGTTCCTGG.CCGTGCC.CCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTg                                              <  1:2108163/101‑1 (MQ=255)
                                                      ccGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGG.CCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATc                                           >  2:3893892/1‑101 (MQ=255)
                                                      ccGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGG.CCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATc                                           <  1:77948/101‑1 (MQ=255)
                                                       cGACCCCGGGCGAGGTCGCCCCCGGGGCCTCGTTCCTGG.CGGCG.AGCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATcc                                          <  1:1362624/101‑1 (MQ=255)
                                                        gACCCAGGGCGAGGTCGCCCTCTTGGCCGCGTTCCTGG.CCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCg                                         <  2:934873/101‑1 (MQ=255)
                                                        gACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGG.CCGTGCC.CCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCg                                         <  1:2249457/101‑1 (MQ=255)
                                                        gACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGG.CCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCg                                         >  1:2571728/1‑101 (MQ=255)
                                                        gACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGG.CCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCg                                         >  2:3629522/1‑101 (MQ=255)
                                                          cccAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGG.CCGTGCC.CCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGAt                                       <  2:4426413/101‑1 (MQ=255)
                                                            cAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGG.CCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGa                                     >  1:509127/1‑101 (MQ=255)
                                                             aGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGG.CCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGAc                                    <  2:814654/101‑1 (MQ=255)
                                                               ggCGAGGTCGCCCTCTGGGCCGCGTTCCTGG.CCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCGGCAAAAGGCCCGATCCGATGAccc                                  >  2:1969992/1‑101 (MQ=255)
                                                                gCGAGGTCGCCCTCTGGGCCGCGTTCCTGG.CCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGAcccc                                 >  2:1271072/1‑101 (MQ=255)
                                                                 cGAGGTCGCCCTCTGGGCCGCGTTCCTGG.CCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCt                                >  2:2851720/1‑101 (MQ=255)
                                                                  gAGGTCGCCCTCTGGGCCGCGTTCCTGG.CCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCtt                               >  2:1226571/1‑101 (MQ=255)
                                                                  gAGGTCGCCCTCTGGGCCGCGTTCCTGG.CCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCtt                               >  1:3439416/1‑101 (MQ=255)
                                                                  gAGGTCGCCCTCTGGGCCGCGTTCCTGG.CCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCtc                               >  1:665920/1‑100 (MQ=255)
                                                                    ggTCGCCCTCTGGGCCGCGTTCCTGG.CCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTcc                             <  2:2295068/101‑1 (MQ=255)
                                                                    ggTCGCCCTCTGGGCCGCGTTCCTGG.CCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTcc                             <  1:4205291/101‑1 (MQ=255)
                                                                      tCGCCCTCTGGGCCGCGTTCCTGG.CCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGc                           >  2:4148612/1‑101 (MQ=255)
                                                                      tCGCCCTCTGGGCCGCGTTCCTGG.CCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGc                           >  1:2728107/1‑101 (MQ=255)
                                                                         cccTCTGGGCCGCGTTCCTGG.CCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGcaca                        >  2:3493243/1‑101 (MQ=255)
                                                                         cccTCTGGGCCGCGTTCCTGG.CCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGcaca                        <  1:2801694/101‑1 (MQ=255)
                                                                          ccTCTGGGCCGCGTTCCTGG.CCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACaa                       >  2:2339909/1‑101 (MQ=255)
                                                                           ctctGGGCCGCGTTCCTGG.CCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAg                      >  2:3513080/1‑101 (MQ=255)
                                                                             ctGGGCCGCGTTCCTGG.CCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAgcg                    >  1:238656/1‑101 (MQ=255)
                                                                             ctGGCCCGGGTTCCTGG.CCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAgcg                    <  1:2269260/101‑1 (MQ=255)
                                                                               ggcccGCGTTCCTGGCCCGGC.C.CCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAGCGcc                  <  2:511939/98‑1 (MQ=255)
                                                                                ggCCGCGTTCCTGG.CCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAGCGCCg                 >  2:949635/1‑101 (MQ=255)
                                                                                ggCCGCGTTCCTGG.CCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAGCGCCg                 <  2:2785334/101‑1 (MQ=255)
                                                                                   cgcgTTCCTGG.CCGTGCC.CCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAGCGCCGTcc              <  2:665858/101‑1 (MQ=255)
                                                                                   cgcgTTCCTGG.CCGTGCC.CCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAGCGCCGTcc              <  2:2535880/101‑1 (MQ=255)
                                                                                      gTTCCTGG.CCGTGCC.CCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAGCGCCGTCCggg           <  1:1531786/101‑1 (MQ=255)
                                                                                        tCCTGG.CCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAGCGCCGTCCGGgcg         >  1:3221798/1‑101 (MQ=255)
                                                                                         ccTGG.CCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAGCGCCGTCCGGgcgc        >  2:4473859/1‑101 (MQ=255)
                                                                                           tGG.CCGTG.ACCCTGCCGCTCTGCCTGCGCCCCGCGCCCGCCCCCCTTCCGCATAAGGCCTGATCCGATGACCCCTTCCGCACAAGCGCCGTCCGGGCGcct      >  1:2650559/1‑101 (MQ=255)
                                                                                            gg.CCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAGCGCCGTCCGGGCGcctc     >  2:3010805/1‑101 (MQ=255)
                                                                                               ccGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAGCGCCGTCCGGGCGcctcct   >  2:2467977/1‑101 (MQ=255)
                                                                                                      |                                                                                               
AATGCCTCGGTCGCGGGCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGG.CCGTG.A.CCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAGCGCCGTCCGGGCGCCTCCTT  >  NC_014034/3258864‑3259058

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG