Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,485,049 +C 1.9% coding (12/654 nt) rnfG → electron transport complex protein RnfG

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,485,0491.→C 1.9% 4.7 0.0 ‑0.1207coding (12/654 nt)rnfGelectron transport complex protein RnfG
Reads supporting (aligned to +/- strand):  new base (2/2):  ref base (99/104):  total (101/106)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.37e-01

Only 100 of 261 total aligned reads displayed.

ATCGAAACCTACATCCGGCCGCGGATCTTCGGACGGTCCCGCACCGGCAAGCCGCTTGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGA.CACG..CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTCAAGGCCTCGCCTCTGGCGCACGGGATCATGCTGGCGATGTTTGCGCTGGTCACCGCGGTGCTGCTGG  >  NC_014034/3484949‑3485149
                                                                                                      |                                                                                                     
  caaacccTACATCCGGCCGCGGATCTTCGGACGGTCCCGCACCGGCAAGCCGCTTGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGA.CACG..cc                                                                                                    <  1:3324966/96‑1 (MQ=255)
  cGAAACCTACATCCGGCCGCGGATCTTCGGACGGTCCCGCACCGGCAAGCCGCTTGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGA.CACG..cc                                                                                                    >  1:762027/1‑101 (MQ=255)
   gAAGCCTACACCCGCCCGCGGATCTTCGGACGGTCCCGCACCGGCAAGCCGCTTGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGA.CACG..ccc                                                                                                   <  2:2244119/101‑1 (MQ=255)
   gAAACCTACATCCGGCCGCGGATCTTCGGACGGTCCCGCACCGGCAAGCCGCTTGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGA.CACG..ccc                                                                                                   >  1:3592233/1‑101 (MQ=255)
    aaaCCTACATCCGGCCGCGGATCTTCGGACGGTCCCGCACCGGCAAGCCGCTTGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGA.CACG..cccc                                                                                                  >  2:1851340/1‑101 (MQ=255)
      aCCTACATCCGGCCGCGGATCTTCGGACGGTCCCGCACCGGCAAGCCGCTTGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGA.CACG..cccccc                                                                                                >  2:4529493/1‑101 (MQ=255)
         tACATCCGGCCGCGGATCTTCGGACGGTCCCGCACCGGCAAGCCGCTTGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGA.CACG..ccccccccc                                                                                             >  2:2492892/1‑101 (MQ=255)
         tACATCCGGCCGCGGATCTTCGGACGGTCCCGCACCGGCAAGCCGCTTGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGA.CACG..ccccccccc                                                                                             >  1:662748/1‑101 (MQ=255)
         accATCCGGCCGCGAATCTCCGGGCGGTCCCGCACCGGCAAGCCGCTTGGCGCCGCCAACCCCGCGAAGGGAGCGCAGAAATGACCGA.CACG..ccccccccc                                                                                             <  1:4220728/99‑1 (MQ=255)
          aCACCCGGCCGCGGATCTTCGGACGGTCCCGCACCGGCAAGCCGCTTGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGA.CACG..CCCCCCCCCg                                                                                            <  1:3539334/101‑1 (MQ=255)
              ccGGCCGGGGATCTTCGGACGGTCCCGCACCGGCAAGCCGCTTGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGA.CACG..CCCCCCCCCGaaaa                                                                                        <  2:1342531/101‑1 (MQ=255)
               cGGCCGCGGATCTTCGGACGGTCCCGCACCGGCAAGCCGCTTGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGA.CACG..CCCCCCCCCGaaaaa                                                                                       <  2:548955/101‑1 (MQ=255)
                ggCCGCGGACCTTAGGACGGTCCCGCACCGGCAAGCCGCTTGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGA.CCCG..CCCCCCCCCGAAAAAc                                                                                      <  1:3123440/101‑1 (MQ=255)
                  ccGCGGATCTTCGGACGGTCCCGCACCGGCAAGCCGCTTGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGA.CACG..CCCCCCCCCGAAAAACCg                                                                                    <  1:1854702/101‑1 (MQ=255)
                   cgcgGATCTTCGGACGGTCCCGCACCGGCAAGCCGCTTGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGA.CACG..CCCCCCCCCGAAAAACCGa                                                                                   >  2:4261758/1‑101 (MQ=255)
                    gcgGGTCTTCGGAGGGTCCCGCCCCGGCAAGCCGCTTGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGA.CACG..CCCCCCCCCGAAAAACCGaa                                                                                  <  1:1292137/101‑1 (MQ=255)
                    gcgGATCTTCGGACGGTCCCGCCCCGGCAAGCCGCTTGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGA.CACG..CCCCCCCCCGAAAAACCGaa                                                                                  <  2:1154210/101‑1 (MQ=255)
                    gcgGATCTTCGGACGGTCCCGCACCGGCAAGCCGCTTGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGA.CACG..CCCCCCCCCGAAAAACCGaa                                                                                  <  1:785605/101‑1 (MQ=255)
                     cgGATCTTCGGACGGTCCCGCACCGGCAAGCCGCTTGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGA.CACG..CCCCCCCCCGAAAAACCGaaa                                                                                 >  1:4068017/1‑101 (MQ=255)
                        aTCTTCGGACGGTCCCGCACCGGCAAGCCGCTTGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGA.CACG..CCCCCCCCCGAAAAACCGAAACtc                                                                              >  2:2738976/1‑100 (MQ=255)
                        aTCTTCGGACGGTCCCGCACCGGCAAGCCGCTTGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGA.CACG..CCCCCCCCCGAAAAACCGAAACTg                                                                              <  1:4648493/101‑1 (MQ=255)
                           ttCGGACGGCCCCGCCCCGGCAAGCCGCTTGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGA.CACG..CCCCCACCCGAAAAACCGAAACTGccc                                                                           <  1:3435063/101‑1 (MQ=255)
                             cGGCCGGTCCCGCACCGGCAAGCCGCTTGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGA.CACG..CCCCCCCCCGAAAAACCGAAACTGCCCTg                                                                         <  1:586319/101‑1 (MQ=255)
                             cGGACGGTCCCGCACCGGCAAGCCGCTTGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGA.CACG..CCCCCCCCCGAAAAACCGAAACTGCCCTg                                                                         >  2:430999/1‑101 (MQ=255)
                              ggACGGTCCCGCACCGGCAAGCCGCTTGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGA.CACG..CCCCCCCCCGAAAAACCGAAACTGCCCTgg                                                                        >  2:124363/1‑101 (MQ=255)
                               gACGGGCCCGCCCCGGCAAGCCGCTTGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGA.CACG..CCCCCCCCCGAAAAACCGAAACTGCCCTGGt                                                                       <  1:1020670/101‑1 (MQ=255)
                                aCGGTCCCGCACCGGCAAGCCGCTTGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGA.CACG..CCCCCCCCCGAAAAACCAAACCTGCCCGGGtc                                                                      >  2:1872246/1‑100 (MQ=255)
                                aCGGCCCCGCCCCGGCAAGCCGCTTGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGA.CACG..CCCCCCCCCGAAAAACCGAAACTGCCCTGGtt                                                                      <  2:3936870/101‑1 (MQ=255)
                                 cGGTCCCGCACCGGCAAGCCGCTTGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGA.CACG..CCCCCCCCCGGAAAACCGAAACTGCCCTGGCTc                                                                     >  2:791107/1‑101 (MQ=255)
                                 cGGTCCCGCACCGGCAAGCCGCTTGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGA.CACG..CCCCCCCCCGAAAAACCGAAACTGCCCTGGtcc                                                                     >  2:4400632/1‑99 (MQ=255)
                                 cGGCCCCGCCCCGGGAAGCCGCTTGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGA.CACG..CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTc                                                                     <  2:3829877/101‑1 (MQ=255)
                                 cGGCCCCGCCCCGGCAAGCCGCTTGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGA.CACG..CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTc                                                                     <  1:1599987/101‑1 (MQ=255)
                                  ggTCCCGCCCCTGCAAGCCGCTTGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGA.CCCG..CCCCCCCCCGAAAAACCGAAACTGCCCTGGCTCa                                                                    <  2:1516131/101‑1 (MQ=255)
                                  ggTCCCGCACCGGCAAGCCGCTTGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGA.CACG..CCCCCCCCCGACGAACCGAAACTGCCCTGGTTCa                                                                    >  1:1165553/1‑101 (MQ=255)
                                   gccccGCACCGGCAAGCCGCTTGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGA.CACG..CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTCaa                                                                   <  2:939800/99‑1 (MQ=255)
                                   gccccGCACCGGCAAGCCGCTTGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGA.CACG..CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTCaa                                                                   <  1:1007424/99‑1 (MQ=255)
                                     cccGCACCGGCAAGCCGCTTGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGA.CACG..CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTCAAgg                                                                 >  2:4076785/1‑101 (MQ=255)
                                       cGCACCGGCAAGCCGCTTGGCGCCGCCAAACCCGCGAAGGGGGCGCAGAAATGACCGA.CACG..CCCCCCCCCGAAAAACCGAAACTGCCCTGGGTCAAGgca                                                               >  1:3671285/1‑100 (MQ=255)
                                         ccccGGCAAGCCGCTTGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGA.CACG..CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTCAAGgcctc                                                             <  1:2233850/99‑1 (MQ=255)
                                          cccGGCAAGCCGCTTGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGA.CACG..CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTCAAGgcctcg                                                            <  1:4532484/100‑1 (MQ=255)
                                          cccGGCAAGCCGCTTGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGA.CACG..CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTCAAGgcctcg                                                            <  2:2327330/100‑1 (MQ=255)
                                          cccGGCAAGCCGCTTGGCGCCCCCAAACCCGCGAAGGGAGCGCAGAAATGACCGA.CCCGC.CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTCAAGgcctc                                                             <  1:4383572/100‑1 (MQ=255)
                                          aCCGGCAAGCCGCTTGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGA.CACG..CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTCAAGgcctcg                                                            >  1:145735/1‑101 (MQ=255)
                                               cAAGCCGCTTGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGA.CACG..CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTCAAGGCCTCGCctct                                                       <  2:3545992/101‑1 (MQ=255)
                                               cAAGCCGCTTGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGA.CACG..CCCCCCCCCCAAATACCGAAACTGCCCTGGTTCAAGgcctccccccc                                                       >  1:3982123/1‑98 (MQ=255)
                                                 aGCCGCTTGGCGCCGCCAAGCCCGCGAAGGGAGCGCAGAAATGACCGA.CACG..CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTCAAGGCCTCGCCTCTgg                                                     <  2:705113/101‑1 (MQ=255)
                                                 aGCCGCCTGGCCCCCCCCAACCCGCGAAGGGAGCGCAGAAATGACCGACCCCC..CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTCAAGGCCTCGCCTCTg                                                      <  1:3948439/101‑1 (MQ=255)
                                                   ccGCTTGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGA.CACG..CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTCAAGGCCTCGCCTCTGgcg                                                   <  1:3069755/101‑1 (MQ=255)
                                                     gCTTGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGA.CACGCCCCCCCCCCCGAAAAACCGAAACGGCCCTGGTTCAAGGCCCCCCCCCTGgcc                                                   >  1:1479942/1‑100 (MQ=255)
                                                     gCTTGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGA.CACG..CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTCAAGGCCTCGCCTCTGGCGCa                                                 >  2:2976465/1‑101 (MQ=255)
                                                      cTTGGCGCCGCCAACCCCGCGAAGGGAGCGCAGAAAGGACCGA.CACG..CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTCAAGGCCTCGCCTCTGGCGCAc                                                <  2:2118049/101‑1 (MQ=255)
                                                      cTTGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGA.CACG..CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTCAAGGCCTCGCCTCTGGCGCAc                                                <  1:138217/101‑1 (MQ=255)
                                                      cTTGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGA.CACG..CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTCAAGGCCTCGCCTCTGGCGCAc                                                <  1:4559258/101‑1 (MQ=255)
                                                       ttGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGA.CACG..CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTCAAGGCCTCGCCTCTGGCGCACg                                               <  2:2390166/101‑1 (MQ=255)
                                                       ttGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGA.CACG..CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTCAAGGCCTCGCCTCTGGCGCACg                                               <  1:925708/101‑1 (MQ=255)
                                                       ttGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGA.CACG..CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTCAAGGCCTCGCCTCTGGCGCACg                                               <  1:726654/101‑1 (MQ=255)
                                                        tGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGA.CACG..CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTCAAGGCCTCGCCTCTGGCGCACgg                                              <  1:3480187/101‑1 (MQ=255)
                                                          gcgcCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGA.CACG..CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTCAAGGCCTCGCCTCTGGCGCACGGGa                                            <  1:3225182/101‑1 (MQ=255)
                                                            gccgccAAACCCGCGAAGGGAGCGCAGAAATGACCGA.CACG..CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTCAAGGCCTCGCCTCTGGCGCACGGGATc                                          <  1:85728/101‑1 (MQ=255)
                                                              cgccAAACCCGCGAAGGGAGCGCAGAAATGACCGA.CACG..CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTCAAGGCCTCGCCTCTGGCGCACGGGATCAt                                        <  1:2863465/101‑1 (MQ=255)
                                                               gccAAACCCGCGAAGGGAGCGCAGAAATGACCGA.CACC..CCCCCCCCCGAAAAACCGAAACTGCCCTGGGTCAAGGCCTCGCCTCTGGCGCCCGGGATCATg                                       >  1:2086856/1‑101 (MQ=255)
                                                                ccAAACCCGCGAAGGGAGCGCAGAAATGACCGA.CACG..CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTCAAGGCCTCGCCTCTGGCGCACGGGATCATGc                                      >  1:2167644/1‑101 (MQ=255)
                                                                 cAAACCCGCGAAGGGAGCGCAGAAATGACCGA.CCCC..CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTCAAGGCCTCGCCTCTGGCGCACGGGATCATGCt                                     <  1:4479790/101‑1 (MQ=255)
                                                                  aaaCCCGCGAAGGGAGCGCAGAAATTACCGA.CACG..CCCCCCCCCGAAAAGACGAAACCGCGCTGGGTGCAGGACGCGCCTCTCGCGGACGGGAGCCTGcgg                                    >  2:2034344/1‑99 (MQ=255)
                                                                   aaCCCGCGAAGGGAGCGCAGAAATGACCGA.CACG..CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTCAAGGCCTCGCCTCTGGCGCACGGGATCATGCTgg                                   <  2:3036210/101‑1 (MQ=255)
                                                                       cgcgAAGGGAGCGCAGAAATGCCCGA.CACC..CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTCAAGGCCTCGCCTCTGGCGCACGGGATCATGCTGGCGAt                               <  1:2056613/101‑1 (MQ=255)
                                                                         cgAAGGGAGCGCAGAAATGACCGA.CACG..CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTCAAGGCCTCGCCTCTGGCGCACGGGATCATGCTGGCGATGt                             >  1:3746250/1‑101 (MQ=255)
                                                                         cgAAGGGAGCGCAGAAATGACCGA.CACG..CCCCCCCCCGAAAAACCGAAACCGCCCTGGTTCAAGGCCTCGCCTCTGGCGCACGGGATCATGCTGGCGATGt                             >  2:4033533/1‑101 (MQ=255)
                                                                          gAAGGGAGCGCAGAAATGACCGA.CACGC.CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTCAAGGCCTCGCCTCTGGCGCACGGGATCATGCTGGCGATGt                             <  1:155504/101‑1 (MQ=255)
                                                                          gAAGGGAGCGCAGAAATGACCGA.CACG..CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTCAAGGCCTCGCCTCTGGCGCACGGGATCATGCTGGCGATGtt                            <  2:3404075/101‑1 (MQ=255)
                                                                          gAAGGGAGCGCAGAAATGACCGA.CACG..CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTCAAGGCCTCGCCTCTGGCGCACGGGATCATGCTGGCGATGtt                            <  1:190863/101‑1 (MQ=255)
                                                                           aaGGGAGCGCAGAAATGACCGA.CACG..CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTCAAGGCCTCGCCTCTGGCGCACGGGATCATGCTGGCGATGttt                           <  2:45052/101‑1 (MQ=255)
                                                                           aaGGGAGCGCAGAAATGACCGA.CACG..CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTCAAGGCCTCGCCTCTGGCGCACGGGATCATGCTGGCGATGttt                           <  2:594156/101‑1 (MQ=255)
                                                                             gggAGCGCAGAAATGACCGA.CACG..CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTCAAGGCCTCGCCTCTGGCGCACGGGATCATGCTGGCGATGTTTgc                         <  1:558198/101‑1 (MQ=255)
                                                                             gggAGCGCAGAAATGACCGA.CACG..CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTCAAGGCCTCGCCTCTGGCGCACGGGATCATGCTGGCGATGTTTgc                         >  2:1090203/1‑101 (MQ=255)
                                                                              ggAGCGCAGAAATGACCGA.CACG..CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTCAAGGCCTCGCCTCTGGCGCACGGGATCATGCTGGCGATGTTTgcg                        >  2:2188182/1‑101 (MQ=255)
                                                                                  cgcAGAAATGACCGA.CACG..CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTCAAGGCCTCGCCTCTGGCGCACGGGATCATGCTGGCGATGTTTGCGCTgg                    >  2:1596475/1‑101 (MQ=255)
                                                                                  cgcAGAAATGACCGA.CACG..CCCCCCCCCCAAAAAACCAAACTGCCCCGGGTCAAGGGCCCCCCTCCGGGGGCCGGGGTCATGCTGGCGGTGGTTGGCCTgg                    >  1:971568/1‑101 (MQ=255)
                                                                                    cAGAAATGACCGA.CACG..CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTCAAGGCCTCGCCTCTGGCGCACGGGATCATGCTGGCGATGTTTGCGCTGGTc                  <  1:240000/101‑1 (MQ=255)
                                                                                     aGAAATGACCGA.CACG..CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTCAAGGCCTCGCCTCTGGCGCACGGGATCATGCTGGCGATGTTTGCGCTGGTCa                 >  2:1679288/1‑101 (MQ=255)
                                                                                      gAAATGACCGA.CACG..CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTCAAGGCCTCGCCTCTGGCGCACGGGATCATGCTGGCGATGTTTGCGCTGGTCAc                >  1:4100843/1‑101 (MQ=255)
                                                                                        aaTGACCGA.CACG..CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTCAAGGCCTCGCCTCTGGCGCACGGGATCATGCTGGCGATGTTTGCGCTgagatcgg              >  2:1084449/1‑94 (MQ=255)
                                                                                        aaTGACCGA.CACG..CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTCAAGGCCTCGCCTCTGGCGCACGGGATCATGCTGGCGATGTTTGCGCTGGTCACcg              <  1:431036/101‑1 (MQ=255)
                                                                                         aTGACCGA.CACG..CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTCAAGGCCTCGCCTCTGGCGCACGGGATCATGCTGGCGATGTTTGCGCTGGTCACcgc             <  2:2066695/101‑1 (MQ=255)
                                                                                         aTGACCGA.CACG..CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTCAAGGCCTCGCCTCTGGCGCACGGGATCATGCTGGCGATGTTTGCGCTGGTCACcgc             >  1:2575192/1‑101 (MQ=255)
                                                                                         aTGACCGA.CACG..CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTCAAGGCCTCGCCTCTGGCGCACGGGATCATGCTGGCGATGTTTGCGCTGGTCACcgc             <  2:2870660/101‑1 (MQ=255)
                                                                                          tGACCGA.CACG..CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTCAAGGCCTCGCCTCTGGCGCACGGGATCATGCTGGCGATGTTTGCGCTGGTCACcgcg            >  1:2269221/1‑101 (MQ=255)
                                                                                           gACCGC.CCCG..CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTCAAGGCCTCGCCTCTGGCGCACGGGATCATGCTGGCGATGTTTGCGCTGGTCACCGCgg           <  1:2670467/101‑1 (MQ=255)
                                                                                           gACCGA.CACG..CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTCAAGGCCTCGCCTCTGGCGCACGGGATCATGCTGGCGATGTTTGCGCTGGTCACCGCgg           >  2:3147810/1‑101 (MQ=255)
                                                                                           gACCGA.CACG..CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTCAAGGCCTCGCCTCTGGCGCACGGGATCATGCTGGCGATGTTTGCGCTGGTCACCGCgg           >  2:1313510/1‑101 (MQ=255)
                                                                                             ccGA.CACG..CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTCAAGGCCTCGCCTCTGGCGCACGGGATCATGCTGGCGATGTTTGCGCTGGTCACCGCGGtg         <  2:3467682/101‑1 (MQ=255)
                                                                                              cGA.CACG..CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTCAAGGCCTCGCCTCTGGCGCACGGGATCATGCCGGCGATGTTTGCGCTGGGCACCGCGGggc        >  2:119456/1‑101 (MQ=255)
                                                                                              cGA.CACG..CCCCCCCCCGAAAAACCGAAACTGCCCTGGGTCAAGGCCTCGCCTCTGGCGCACGGGATCATGCTGGCGAGGTTTGCCCTAGGCACCGCGGtga        >  2:1550731/1‑100 (MQ=255)
                                                                                               gA.CCCG..CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTCAAGGCCTCGCCTCTGGCGCACGGGATCATGCTGGCGATGTTTGCGCTGGTCACCGCGGtgct       <  2:3671010/101‑1 (MQ=255)
                                                                                               gA.CACG..CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTCAAGGCCTCGCCTCTGGCGCACGGGATCATGCTGGCGATGTTTGCGCTGGTCACCGCGGtgct       >  1:3820546/1‑101 (MQ=255)
                                                                                                 gcacG..CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTCAAGGCCTCGCCTCTGGCGCACGGGATCATGCTGGCGATGTTTGCGCTGGTCACCGCGGtgctg      <  2:3328715/100‑1 (MQ=255)
                                                                                                    cG..CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTCAAGGCCTCGCCTCTGGCGCACGGGATCATGCTGGCGATGTTTGCGCTGGTCACCGCGGTGctgctg   <  2:531297/101‑1 (MQ=255)
                                                                                                    cG..CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTCAAGGCCTCGCCTCTGGCGCACGGGATCATGCTGGCGATGTTTGCGCTGGTCACCGCGGTGctgctg   >  1:1115412/1‑101 (MQ=255)
                                                                                                    cG..CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTCAAGGCCTCGCCTCTGGCGCACGGGATCATGCTGGCGATGTTTGCGCTGGTCACCGCGGTGctgctg   >  2:2660385/1‑101 (MQ=255)
                                                                                                     g..CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTCAAGGCCTCGCCTCTGGCGCACGGGATCATGCTGGCGATGTTTGCGCTGGTCACCGCGGTGCTGCTgg  >  1:3617570/1‑101 (MQ=255)
                                                                                                      |                                                                                                     
ATCGAAACCTACATCCGGCCGCGGATCTTCGGACGGTCCCGCACCGGCAAGCCGCTTGGCGCCGCCAAACCCGCGAAGGGAGCGCAGAAATGACCGA.CACG..CCCCCCCCCGAAAAACCGAAACTGCCCTGGTTCAAGGCCTCGCCTCTGGCGCACGGGATCATGCTGGCGATGTTTGCGCTGGTCACCGCGGTGCTGCTGG  >  NC_014034/3484949‑3485149

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 24 ≤  ATCG  < 32 ≤  ATCG  < 40 ≤  ATCG