Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,532,987 +G 89.3% intergenic (‑6/‑19) RCAP_rcc03340 ← / → RCAP_rcc03341 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,532,9871.→G89.3% 68.2 ‑0.2 ‑0.7214intergenic (‑6/‑19)RCAP_rcc03340/RCAP_rcc03341hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (98/93):  ref base (11/12):  total (109/105)
Fisher's exact test strand distribution p-value = 8.27e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.14e-01

Only 100 of 241 total aligned reads displayed.

AGCGGGGAAAGCGGCGCCTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTC.G.GGGCGATCTGCGCCATCGCGGCGCAAAGCTGC.G.GCCC.GCCCTTCGATCCAGTGCAGCCAACCGTCCTCGACCGCAACCGTCGCCCCGCACAGAT.CTGCGCGCCCGGCAGGGCCGCCAGATCGTGC  >  NC_014034/3532888‑3533080
                                                                                                      |                                                                                                
aGCGGGGAAAGCGGCGCCTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGG.GGGCGATCTGCGCCATCGCGGCGCAAAGCTGc                                                                                                   <  2:774589/101‑1 (MQ=255)
aGCGGGGAAAGCGGCGCCTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGG.GGGCGATCTGCGCCATCGCGGCGCAAAGCTGc                                                                                                   <  1:2952517/101‑1 (MQ=255)
aGCGGGGAAAGCGGCGCCTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGG.GGGCGATCTGCGCCATCGCGGCGCAAAGCTGc                                                                                                   <  2:3304221/101‑1 (MQ=255)
 ggggggAAGCCGGCCCCTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGG.GGGCGATCTGCGCCATCGCGGCGCAAAGCTGC.g                                                                                                 <  2:2181837/99‑1 (MQ=255)
 gCGGGGAAAGCGGCGCCTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGG.GGGCGATCTGCGCCATCGCGGCGCAAAGCTGC.g                                                                                                 <  1:325718/101‑1 (MQ=255)
 gCGGGGAAAGCGGCGCCTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGG.GGGCGATCTGCGCCATCGCGGCGCAAAGCTGC.g                                                                                                 >  1:1836019/1‑101 (MQ=255)
   ggggAAAGCGGCGCCTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGG.GGGCGATCTGCTCCATCGCGGCGCAAAGCTGC.g.gg                                                                                              >  1:1242761/1‑100 (MQ=255)
    gggAAAGCGGCGCCTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGG.GGGCGATCTGCGCCATCGCGGCGCAAAGCTGC.g.ggc                                                                                             >  2:344697/1‑99 (MQ=255)
     ggAAAGCGGCGCCTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGG.GGGCGATCTGCGCCATCGCGGCGCAAAGCTGC.G.ggcc                                                                                            >  2:2432328/1‑101 (MQ=255)
       aaaGCGGCGCCTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGG.GGGCGATCTGCGCCATCGCGGCGCAAAGCTGC.G.ggcccg                                                                                          >  2:3593128/1‑99 (MQ=255)
       aaaGCGGCGCCTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGG.GGGCGATCTGCGCCATCGCGGCGCAAAGCTGC.G.ggcccg                                                                                          <  1:216873/101‑3 (MQ=255)
        aaGCGGCGCCTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGG.GGGCGATCTGCGCCATCGCGGCGCAAAGCTGC.G.ggcccgg                                                                                         <  2:2757497/101‑4 (MQ=255)
          gcggcgCCTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGG.GGGCGATCTGCGCCATCGCGGCGCAAAGCTGC.G.ggcccggcc                                                                                       <  1:1545268/101‑6 (MQ=255)
           cggcgCCTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGG.GGGCGATCTGCGCCATCGCGGCGCAAAGCTGC.G.ggcccggccc                                                                                      >  1:2846700/1‑95 (MQ=255)
           cggcgCCTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGG.GGGCGATCTGCGCCATCGCGGCGCAAAGCTGC.G.ggcccggccc                                                                                      <  1:1991704/101‑7 (MQ=255)
           cggcgCCTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGG.GGGCGATCTGCGCCATCGCGGCGCAAAGCTGC.G.ggcccggccc                                                                                      >  2:63893/1‑95 (MQ=255)
           cggcgCCTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGG.GGGCGATCTGCGCCATCGCGGCGCAAAGCTGC.G.ggcccggccc                                                                                      <  1:1057371/101‑7 (MQ=255)
              ccccTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGG.GGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCGGCCCTTc                                                                                    <  2:1339548/99‑1 (MQ=255)
                ccTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGG.GGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCGGCCCTTCGa                                                                                  >  1:248135/1‑101 (MQ=255)
                ccTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGG.GGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCGGCCCTTCGa                                                                                  >  1:3485063/1‑101 (MQ=255)
                  tGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGG.GGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCGGCCCTTCGATc                                                                                >  1:2395463/1‑101 (MQ=255)
                  tGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGG.GGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCGGCCCTTCGATc                                                                                >  2:1602748/1‑101 (MQ=255)
                   gTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGG.GGGCGATATGCACCAGAGCGGCGCAAAGCTGCGG.GCCCGGCCCTTCGATcc                                                                               >  2:1086310/1‑101 (MQ=255)
                   gTCGGTCCAGCCGACCGAAAGAAGCCGCGCCTCGGCCAGCCGGGGGTCGG.GGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCGGCCCTCCGATcc                                                                               >  2:2023231/1‑101 (MQ=255)
                    tCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGG.GGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCGGCCCTTCGATCCa                                                                              >  2:4245676/1‑101 (MQ=255)
                     cGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGG.GGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCGGCCCTTCGATCCAg                                                                             >  1:4120202/1‑101 (MQ=255)
                     cGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGG.GGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCGGCCCTTCGATCCAg                                                                             <  2:4294798/101‑1 (MQ=255)
                     cGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGG.GGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCGGCCCTTCCATCCAg                                                                             >  2:1203867/1‑101 (MQ=255)
                      ggTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGG.GGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCGGCCCTTCGATCCAGt                                                                            >  2:4391845/1‑101 (MQ=255)
                      ggTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGG.GGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCGGCCCTTCGATCCAGt                                                                            >  1:585178/1‑101 (MQ=255)
                       gTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGG.GGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCGGCCCTTCGATCCAGTg                                                                           >  2:2006268/1‑101 (MQ=255)
                        tCCATCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGG.GGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCGGCCCTTCGATCCAGTGc                                                                          >  1:2459188/1‑101 (MQ=255)
                        tCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGG.GGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCGGCCCTTCGATCCAGTGc                                                                          <  1:4586246/101‑1 (MQ=255)
                         ccAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGG.GGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCGGCCCTTCGATCCAGTGCa                                                                         >  2:2830734/1‑101 (MQ=255)
                         ccAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGG.GGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCGGCCCTTCGATCCAGTGCa                                                                         >  1:4612433/1‑101 (MQ=255)
                          cAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGG.GGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCGGCCCTTCGATCCAGTGCAg                                                                        >  1:3210942/1‑101 (MQ=255)
                           aGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGG.GGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCGGCCCTTCGATCCAGTGCAGc                                                                       <  1:4357179/101‑1 (MQ=255)
                            gCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGG.GGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCGGCCCTTCGATCCAGTGCAGcc                                                                      <  2:3611756/101‑1 (MQ=255)
                              cgaccgaAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGG.GGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCGGCCCTTCGATCCAGTGCAGCCag                                                                    >  2:1635863/1‑100 (MQ=255)
                               gaccgaAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGG.GGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCGGCCCTTCGATCCAGTGCAGCCagc                                                                   >  1:2700215/1‑99 (MQ=255)
                                cccgaAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGG.GGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCGGCCCTTCGATCCAGTGCAGCCAGcc                                                                  <  2:1478524/100‑1 (MQ=255)
                                   gaAAGCAGCCGCGCCTCGGCCAGCCGGGGGTCGG.GGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTc                                                               <  1:550640/101‑1 (MQ=255)
                                      agcagcCGCGCCTCGGCCAGCCGGGGGTCGG.GGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTc                                                            <  1:2382497/101‑1 (MQ=255)
                                       gcagcCGCGCCTCGGCCAGCCGGGGGTCGG.GGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCg                                                           <  2:4656383/101‑1 (MQ=255)
                                         agcCGCGCCTCGGCCAGCCGGGGGTCGG.GGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGAc                                                         >  1:1395731/1‑101 (MQ=255)
                                         agcCGCGCCTCGGCCAGCCGGGGGTCGG.GGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGAc                                                         <  1:3289812/101‑1 (MQ=255)
                                           ccGCGCCGCGGCCAGCAGGCGGTC.GCGGGCGATCTGCGCCATCGCGGCGCAAAGCGCCGG.GCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCg                                                       <  2:487278/101‑1 (MQ=255)
                                            cgcgCCTCGGCCAGCCGGGGGTCGG.GGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGc                                                      >  1:304003/1‑101 (MQ=255)
                                             gcgcCTCGGCCAGCCGGGGGTCGG.GGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCa                                                     <  1:2432494/101‑1 (MQ=255)
                                               gcCTCGGCCAGCCGGGGGTCGG.GGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAAc                                                   <  2:2255213/101‑1 (MQ=255)
                                                ccTCGGCCAGCCGGGGGTCGG.GGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAAcc                                                  <  2:4021723/101‑1 (MQ=255)
                                                ccTCGGCCAGCCGGGGGTCGG.GGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAAcc                                                  <  2:4343147/101‑1 (MQ=255)
                                                 cTCGGCCAGCCGGGGGTCGG.GGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCg                                                 <  1:394898/101‑1 (MQ=255)
                                                  tCGGCCAGCCGGGGGGCGG.GGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCGGCCCTTCGATCCAGTGTAGCCAGCCGTCTTCGACCGCAACCGt                                                >  2:3130109/1‑101 (MQ=255)
                                                   cGGCCAGCCGGGGGTCGG.GGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTc                                               >  2:2281675/1‑101 (MQ=255)
                                                    ggCCAGCCGGGGGTCGG.GGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCg                                              <  2:4486782/101‑1 (MQ=255)
                                                       cAGCCGGGGGTCGG.GGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGccc                                           >  2:1707833/1‑101 (MQ=255)
                                                        aGCCGGGGGTCGG.GGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGcccc                                          >  1:2010820/1‑101 (MQ=255)
                                                        aGCCGGGGGTCGG.GGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGcccc                                          <  2:2846484/101‑1 (MQ=255)
                                                          ccGGGGGTCGG.GGGCGTTCGGGGCCATCGCGGCGCAAAGCTGCGG.GCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGc                                        <  1:1709747/101‑1 (MQ=255)
                                                            gggggTCGG.GGGCGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGcac                                      >  2:3676376/1‑101 (MQ=255)
                                                                gtcgg.gggCGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGAt                                  <  1:1635968/97‑1 (MQ=255)
                                                                gtcgg.gggCGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGAt                                  <  1:2960014/97‑1 (MQ=255)
                                                                gtcgg.gggCGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGAt                                  <  2:4612132/97‑1 (MQ=255)
                                                                 tcgg.gggCGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGAT.c                                >  2:2406239/4‑101 (MQ=255)
                                                                 tcgg.gggCGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGAT.c                                >  2:1096050/4‑101 (MQ=255)
                                                                  cgg.gggCGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGAT.cc                               >  2:2686366/3‑100 (MQ=255)
                                                                  cgg.gggCGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGAT.cc                               <  2:3484805/99‑2 (MQ=255)
                                                                   gg.gggCGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCCCAGAT.cct                              >  1:1585589/2‑99 (MQ=255)
                                                                   gg.gggCGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGAT.cct                              >  1:1316232/2‑99 (MQ=255)
                                                                   gg.gggCGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGAT.cct                              >  2:1758442/2‑99 (MQ=255)
                                                                   gg.gggCGATCTGCGCCANCGCGGCGCAAAGCTGCGG.GCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGAT.cct                              <  2:1882903/100‑3 (MQ=255)
                                                                    g.gggCGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGAT.cctg                             >  2:877976/1‑98 (MQ=255)
                                                                    g.gggCGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGAT.cctg                             <  1:4392137/101‑4 (MQ=255)
                                                                    g.gggCGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGAT.cctg                             >  2:4083898/1‑98 (MQ=255)
                                                                    g.gggCGATCTGCGCCATCGCGGAGCAAAGCTGCGG.GCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGAT.cctg                             <  1:4016279/101‑4 (MQ=255)
                                                                       ggCGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGAT.cctgcg                           <  1:36043/101‑6 (MQ=255)
                                                                        gCGATCTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCCTgcgc                           <  2:2203323/101‑1 (MQ=255)
                                                                            ccTGCGCCATCGCGCCGCAAAGCTGCGG.GCCCGGCCCTTCGATCCAGCGCCGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCCTGCGCGccc                       <  1:2101650/100‑1 (MQ=255)
                                                                             cTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCCTGCGCGCCCg                      >  1:90816/1‑101 (MQ=255)
                                                                             cTGCGCCATCGCGGCGCAAAGCTGCGG.GCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGCCGCCCCGCACAGATCCTGCGCGCCCg                      >  2:3926785/1‑101 (MQ=255)
                                                                              tGCGCCATCGCGGCGCAAAGCTGCGG.GCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCCTGCGCGCCCgg                     >  1:69887/1‑101 (MQ=255)
                                                                                cgcCATCGCGGCGCAAAGCTGCGG.GCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCCTGCGCGCCCGGCa                   >  2:903336/1‑101 (MQ=255)
                                                                                 gcCATCGCGGCGCAAAGCTGCGG.GCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCCTGCGCGCCCGGCAg                  >  2:2719544/1‑101 (MQ=255)
                                                                                   cATCGCGGCGCAAAGCTGCGG.GCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCCTGCGCGCCCGGCAggg                <  1:1190694/101‑1 (MQ=255)
                                                                                    aTCGCGGCGCAAAGCTGCGG.GCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCCTGCGCGCCCGGCAGGgc               >  1:1148407/1‑101 (MQ=255)
                                                                                    aTCGCGGCGCAAAGCTGCGG.GCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCCTGCGCGCCCGGCAGGgc               <  1:4273373/101‑1 (MQ=255)
                                                                                    aTCGCGGCGCAAAGCTGCGG.GCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCCTGCGCGCCCGGCAGGgc               <  1:3927070/101‑1 (MQ=255)
                                                                                      cgcgGCGCAAAGCTGCGG.GCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCCTGCGCGCCCGGCAGGgccg             <  2:2154910/101‑1 (MQ=255)
                                                                                      cgcgGCGCAAAGCTGCGG.GCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCCTGCGCGCCCGGCAGGgccg             >  1:3685018/1‑101 (MQ=255)
                                                                                       gcggcgCAAAGCTGCGG.GCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCCTGCGCGCCCGGCAGGgccgc            >  1:971629/1‑101 (MQ=255)
                                                                                        cggcgCAAAGCTGCGG.GCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCCTGCGCGCCCGGCAGGgccgcc           >  1:3399427/1‑101 (MQ=255)
                                                                                         ggcgCAAAGCTGCGG.GCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCCTGCGCGCCCGGCAGGGCCGCCa          <  2:248111/101‑1 (MQ=255)
                                                                                          gcgcAAAGCTGCGG.GCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCCTGCGCGCCCGGCAGGGCCGCCAg         <  1:4666954/101‑1 (MQ=255)
                                                                                          gcgcAAACCTGC.GTGCCCGGCCCTTCGATCTAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCCTGCGCGCCCGGCAGGGCCGCCAg         <  2:3885714/101‑1 (MQ=255)
                                                                                            gcAAAGCTGCGG.GCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCCTGCGCGCCCGGCAGGGCCGCCAGAt       <  2:3747468/101‑1 (MQ=255)
                                                                                             cAAAGCTGCGG.GCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCCTGCGCGCCCGGCAGGGCCGCCAGATc      >  2:394413/1‑101 (MQ=255)
                                                                                                aGCTGCGG.GCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCCTGCGCGCCCGGCAGGGCCGCCAGATCGTg   <  2:297055/101‑1 (MQ=255)
                                                                                                aGCTGCGG.GCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCCTGCGCGCCCGGCAGGGCCGCCAGATCGTg   <  1:4634491/101‑1 (MQ=255)
                                                                                                 gCTGCGG.GCCCGGCCCTTCGATCCAGTGCAGCCAGCCGTCCTCGACCGCAACCGTCGCCCCGCACAGATCCTGCGCGCCCGGCAGGGCCGCCAGATCGTGc  >  1:3825177/1‑101 (MQ=255)
                                                                                                      |                                                                                                
AGCGGGGAAAGCGGCGCCTGTCGGTCCAGCCGACCGAAAGCAGCCGCGCCTCGGCCAGCCGGGGGTC.G.GGGCGATCTGCGCCATCGCGGCGCAAAGCTGC.G.GCCC.GCCCTTCGATCCAGTGCAGCCAACCGTCCTCGACCGCAACCGTCGCCCCGCACAGAT.CTGCGCGCCCGGCAGGGCCGCCAGATCGTGC  >  NC_014034/3532888‑3533080

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG