Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 408,497 +GCAGA 100% intergenic (+115/+33) glnA1 → / ← dapA glutamine synthetase/dihydrodipicolinate synthase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034408,4971.→G100.0% 76.8 147intergenic (+115/+33)glnA1/dapAglutamine synthetase/dihydrodipicolinate synthase
Reads supporting (aligned to +/- strand):  new base (93/29):  ref base (10/14):  total (103/44)
Fisher's exact test strand distribution p-value = 1.29e-03
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00
*NC_014034408,4972.→C100.0% 80.0 147intergenic (+115/+33)glnA1/dapAglutamine synthetase/dihydrodipicolinate synthase
Reads supporting (aligned to +/- strand):  new base (93/29):  ref base (10/15):  total (103/44)
Fisher's exact test strand distribution p-value = 6.40e-04
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.11e-01
*NC_014034408,4973.→A100.0% 77.9 147intergenic (+115/+33)glnA1/dapAglutamine synthetase/dihydrodipicolinate synthase
Reads supporting (aligned to +/- strand):  new base (93/29):  ref base (10/15):  total (103/44)
Fisher's exact test strand distribution p-value = 6.40e-04
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00
*NC_014034408,4974.→G100.0% 80.2 147intergenic (+115/+33)glnA1/dapAglutamine synthetase/dihydrodipicolinate synthase
Reads supporting (aligned to +/- strand):  new base (93/29):  ref base (10/15):  total (103/44)
Fisher's exact test strand distribution p-value = 6.40e-04
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.12e-01
*NC_014034408,4975.→A100.0% 77.9 147intergenic (+115/+33)glnA1/dapAglutamine synthetase/dihydrodipicolinate synthase
Reads supporting (aligned to +/- strand):  new base (93/29):  ref base (10/15):  total (103/44)
Fisher's exact test strand distribution p-value = 6.40e-04
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.61e-01

Only 100 of 156 total aligned reads displayed.

TGCCCCCAGACCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGCAAAGACGGGG....G.....GC.....CCC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTCAAGCGCGGC  >  NC_014034/408397‑408571
                                                                                                         |||||                                                                                
tGCCCCCAGACCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACgggg....g.....g                                                                                 >  1:3521186/1‑101 (MQ=255)
tGCCCCCAGACCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACgggg....g.....g                                                                                 <  2:3073394/101‑1 (MQ=255)
 gCCCCCAGACCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....G.....Gc                                                                                >  2:1794406/1‑101 (MQ=255)
   ccccAGACCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....G.....Gcag                                                                              <  1:1155364/101‑3 (MQ=255)
   ccccAGACCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....G.....Gcag                                                                              >  2:746743/1‑99 (MQ=255)
    cccAGACCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....G.....Gcaga                                                                             <  1:2715690/101‑4 (MQ=255)
    cccAGAACCCCGGGATATTTGCGGCAGGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....G.....Gcaga                                                                             <  2:3677516/101‑4 (MQ=25)
     ccAGACCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....G.....Gcagag                                                                            >  2:782892/1‑97 (MQ=255)
     ccAGACCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....G.....Gcagag                                                                            >  2:4497690/1‑97 (MQ=255)
       agcccccgGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....G.....Gcagagcc                                                                          <  2:4039632/98‑7 (MQ=25)
       agccccccGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....G.....Gcagagcc                                                                          <  2:1697371/98‑7 (MQ=25)
         aCCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....G.....Gcagaggccc                                                                        >  1:2820786/1‑93 (MQ=25)
         aCCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....G.....Gcagagcccc                                                                        >  2:487519/1‑93 (MQ=25)
          cccccGGGATATTTGCGGCAAGATGAAGCCGATGTTCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....G.....Gcagagccccc                                                                       >  1:4172146/1‑92 (MQ=25)
           ccccGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....G.....Gcagagcccccc                                                                      <  1:2206378/101‑11 (MQ=25)
                       tGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....CCC.CCGCACAGGTCGcc                                                         >  1:4465671/1‑101 (MQ=21)
                       tGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....CCC.CCGCACAGGTCGcc                                                         <  1:1908193/101‑1 (MQ=21)
                        gCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....CCC.CCGCACAGGTCGCCa                                                        >  1:3848788/1‑101 (MQ=21)
                        gCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....CCC.CCGCACAGGTCGCCa                                                        >  2:2159111/1‑101 (MQ=21)
                         cGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....CCC.CCGCACAGGTCGCCaa                                                       >  1:4227168/1‑101 (MQ=21)
                         cGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....CCC.CCGCACAGGTCGCCaa                                                       >  1:2749363/1‑101 (MQ=21)
                           gCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....CCC.CCGCACAGGTCGCCaaaa                                                     <  2:4497507/101‑1 (MQ=21)
                             aaGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....CCC.CCGCACAGGTCGCCAAAAgg                                                   <  2:1790302/101‑1 (MQ=21)
                             aaGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....CCC.CCGCACAGGTCGCCAAAAgg                                                   <  1:4496811/101‑1 (MQ=21)
                                 tgtaGCCGATGTGCTTCTTCTTACGGCAAATATCCGGGGGGGAGGCTTGCTGATAGC.AAGACGGGG....GGCAGAGC.....CCC.CCGCACAGGTCGCCAAAAGgaccg                                               <  2:772020/98‑1 (MQ=255)
                                  gAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....CCC.CCGCACAGGTCGCCAAAAGgaccga                                              >  2:3036764/1‑101 (MQ=21)
                                   aaGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....CCC.CCGCACAGGTCGCCAAAAGgaccgac                                             >  1:4235756/1‑101 (MQ=21)
                                   aaGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....CCC.CCGCACAGGTCGCCAAAAGgaccgac                                             <  2:258401/101‑1 (MQ=21)
                                   aaGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....CCC.CCGCACAGGCCGCCAAACGgaccgac                                             >  2:1888544/1‑101 (MQ=18)
                                     gCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....CCC.CCGCACAGGTCGCCAAAAGGACCGACCt                                           >  1:241500/1‑101 (MQ=21)
                                     gCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....G.....GCAGAGACCC.CCGCACAGGTCGCCAAAAGgaccgacat                                           >  1:1521024/1‑99 (MQ=21)
                                       cGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....CCC.CCGCACAGGTCGCCAAAAGGACCGACCTCa                                         >  1:100880/1‑101 (MQ=21)
                                        gATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....CCC.CCGCACAGGTCGCCAAAAGGACCGACCTCAg                                        >  1:3223216/1‑101 (MQ=21)
                                        gATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....CCC.CCGCACAGGTCGCCAAAAGGACCGACCTCAg                                        >  2:1385529/1‑101 (MQ=21)
                                         aTGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....CCC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGa                                       >  2:2700486/1‑101 (MQ=21)
                                         aTGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....CCC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGa                                       >  2:1563419/1‑101 (MQ=21)
                                         aTGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....CCC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGa                                       <  2:1406402/101‑1 (MQ=21)
                                         aTGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....CCC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGa                                       >  2:1393356/1‑101 (MQ=21)
                                          tGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....CCC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGAt                                      >  2:3424927/1‑101 (MQ=21)
                                           gTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....CCC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATc                                     <  1:782967/101‑1 (MQ=21)
                                            tCCTTCATCTTGCCGCAAATTTCCCGGGGGGAGTTTTGCTGCAAGC.AAGACGGGGTGCAGA....GC.....CCC.CCGCACAGGTCGCCAAAAGGACCGACTTCTGATCa                                    <  1:3407254/101‑1 (MQ=12)
                                            tCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....CCC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCa                                    <  1:4497985/101‑1 (MQ=21)
                                             ccTTCATCTTGCCGCAGATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....CCC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAg                                   >  1:4075084/1‑101 (MQ=25)
                                             ccTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....CCC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAg                                   >  2:479483/1‑101 (MQ=21)
                                              cTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....CCC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGc                                  >  1:4389455/1‑101 (MQ=21)
                                              cTTCAGCGTGCCGCAAATGTCCCGGGGGGAGGCTTGCTTAGAGC.AAGACGGGG....GGCCGAGC.....CCC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGc                                  <  1:1531972/101‑1 (MQ=255)
                                               ttCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....CCC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGcc                                 >  2:1651569/1‑101 (MQ=21)
                                                tCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....CCC.CCGCCAAGGTCGCCAAAAGGACCGACCTCAGATCAGccc                                >  1:4010994/1‑101 (MQ=18)
                                                tCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....CCC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGccc                                >  1:766393/1‑101 (MQ=21)
                                                tCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....CCC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGccc                                >  1:3182280/1‑101 (MQ=21)
                                                 cATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....CCC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCg                               >  1:4480274/1‑101 (MQ=21)
                                                  aTCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....CCC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCgg                              >  1:1240321/1‑101 (MQ=21)
                                                  aTCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....CCC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCgg                              >  1:2837731/1‑101 (MQ=21)
                                                   tCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....CCC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGc                             >  2:4056323/1‑101 (MQ=21)
                                                   tCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....CCC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGc                             >  2:4368198/1‑101 (MQ=21)
                                                    cTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....CCC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCg                            >  2:1147166/1‑101 (MQ=25)
                                                    cTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....CCC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCg                            >  2:2230083/1‑101 (MQ=25)
                                                    cTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....CCC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCg                            <  1:1386509/101‑1 (MQ=25)
                                                     ttGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....CCC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCgt                           <  1:2609014/101‑1 (MQ=25)
                                                      tGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....G.....GCAGAG.CCCGCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCgtg                          >  1:3921440/1‑101 (MQ=25)
                                                       gCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....CCC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCgtgt                         <  1:1794525/101‑1 (MQ=25)
                                                         cGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....CCC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTg                       >  2:1826721/1‑101 (MQ=25)
                                                           cAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....CCC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCa                     >  2:4633618/1‑101 (MQ=25)
                                                           cAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....CCC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCa                     <  2:125412/101‑1 (MQ=255)
                                                            aaaTATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....CCC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCAt                    >  1:2365403/1‑101 (MQ=37)
                                                            aaaTATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....CCC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCAt                    >  1:2987627/1‑101 (MQ=37)
                                                            aaaTATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....CCC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCAt                    >  2:3814545/1‑101 (MQ=37)
                                                             aaTATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....CCC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATc                   >  2:1368927/1‑101 (MQ=255)
                                                             aaTATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....CCC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATc                   >  1:1313730/1‑101 (MQ=255)
                                                             aaTAGCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....CCC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATc                   <  2:2528424/101‑1 (MQ=255)
                                                              atatCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....CCC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATcg                  >  2:3408732/1‑101 (MQ=255)
                                                               tatCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....CCC.CCGCACAGGTCGCCAAAAGGACCNNCNNCAGATCAGCCCGGCGTGTTGCATcgc                 >  1:3045995/1‑101 (MQ=255)
                                                               tatCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....CCC.CCGCACAGGTCGCCAAAAGGACCNACCTCAGATCAGCCCGGCGTGTTGCATcgc                 >  1:661998/1‑101 (MQ=255)
                                                               tatCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....CCC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATcgc                 >  2:3566215/1‑101 (MQ=255)
                                                                 tCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....CCC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCgcgg               >  1:2634950/1‑101 (MQ=255)
                                                                  cccGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....CCC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCgcggc              >  1:3322444/1‑101 (MQ=255)
                                                                  cccGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....CCC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCgcggc              <  2:3520928/101‑1 (MQ=255)
                                                                  cccGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....CCC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCgcggc              >  1:4309795/1‑101 (MQ=255)
                                                                  cccGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....CCC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCgcggc              <  2:3049990/101‑1 (MQ=255)
                                                                    cGGGGGGAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....CCC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGt            >  2:4086725/1‑101 (MQ=255)
                                                                     ggggggAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....CCC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTc           >  2:1134767/1‑101 (MQ=255)
                                                                     ggggggAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....CCC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTc           >  2:544094/1‑101 (MQ=255)
                                                                     ggggggAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....CCC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTc           >  2:1253382/1‑101 (MQ=255)
                                                                     ggggggAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....CCC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTc           >  2:2397515/1‑101 (MQ=255)
                                                                     ggggggAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....CCC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTc           >  2:4220298/1‑101 (MQ=255)
                                                                     ggggggAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....CCC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTc           >  2:3254146/1‑101 (MQ=255)
                                                                     ggggggAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....CCC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTc           >  2:3346193/1‑101 (MQ=255)
                                                                      gggggAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....CCC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTCa          >  2:1001673/1‑101 (MQ=255)
                                                                       ggggAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....CCC.CCGCACAGGTCGCCACAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTCaa         >  2:563154/1‑101 (MQ=255)
                                                                       ggggAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....CCC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTCaa         >  2:2269536/1‑101 (MQ=255)
                                                                       ggggAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....CCC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTCaa         >  1:220476/1‑101 (MQ=255)
                                                                         ggAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....CCC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCCTCGCGGCGTCAAgc       >  1:1019174/1‑101 (MQ=255)
                                                                          gAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....CCC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTCAAgcg      >  1:2392474/1‑101 (MQ=255)
                                                                          gAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....CCC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTCAAgcg      >  2:4294398/1‑101 (MQ=255)
                                                                          gAGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....CCC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTCAAgcg      <  2:1930439/101‑1 (MQ=255)
                                                                           aGGCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....CCC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTCAAgcgc     <  1:206225/101‑1 (MQ=255)
                                                                            ggCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....CCC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTCAAGcgcg    <  2:4337392/101‑1 (MQ=255)
                                                                             gCTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....CCC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATAGCGGCGTCAAgagagg   >  2:264864/1‑96 (MQ=255)
                                                                              cTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....CCC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTCAAGCGCGGc  >  1:51870/1‑101 (MQ=255)
                                                                              cTTGCTGAAAGC.AAGACGGGG....GGCAGAGC.....CCC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTCAAGCGCGGc  >  1:609187/1‑101 (MQ=255)
                                                                                                         |||||                                                                                
TGCCCCCAGACCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGCAAAGACGGGG....G.....GC.....CCC.CCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTCAAGCGCGGC  >  NC_014034/408397‑408571

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 40 ≤  ATCG