BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	408,499	+G	2.0%	intergenic (+117/+31)	glnA1 → / ← dapA	glutamine synthetase/dihydrodipicolinate synthase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	408,499	4	.→G	2.0%	2.7 0.0 ‑0.0	149	intergenic (+117/+31)	glnA1/dapA	glutamine synthetase/dihydrodipicolinate synthase
Reads supporting (aligned to +/- strand): new base (2/1): ref base (109/37): total (111/38)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.99e-01

Only 100 of 183 total aligned reads displayed.


GCCCCCAGACCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGCAAAGACGGGGG.....GC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTCAAGCGCGGCCTTGGTCCCGTCGGTCAGTTCGGTCAGC  >  NC_014034/408398‑408599


  ccccAGACCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGG.....Gcag                                                                                                         <  1:1155364/101‑3 (MQ=255)


   cccAGACCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGG.....Gcaga                                                                                                        <  1:2715690/101‑4 (MQ=255)


   cccAGACCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGG.....Gcaga                                                                                                        <  1:1859462/101‑4 (MQ=255)


   ccaagccccccGGGTTATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGG.....Gcaga                                                                                                        <  1:1264218/95‑4 (MQ=25)


    ccAGACCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGG.....Gcagag                                                                                                       >  2:782892/1‑97 (MQ=255)


      agcccccgGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGG.....Gcagagcc                                                                                                     <  2:4039632/98‑7 (MQ=25)


      agccccccGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGG.....Gcagagcc                                                                                                     <  2:1697371/98‑7 (MQ=25)


      agccccccGGGATATTGGGGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGG.....Gcagagcc                                                                                                     <  1:4487608/98‑7 (MQ=25)


      aGACCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCCGAAAGC.AACACGGGGG.....Gcagagcc                                                                                                     >  2:3588934/1‑95 (MQ=25)


       gACCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGG.....Gcagagccc                                                                                                    >  2:4568544/1‑94 (MQ=25)


        aCCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGG.....Gcagagcccc                                                                                                   <  1:2679001/101‑9 (MQ=25)


        aCCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGG.....Gcagagcccc                                                                                                   >  1:1496102/1‑93 (MQ=25)


        aCCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGG.....Gcagagcccc                                                                                                   >  2:487519/1‑93 (MQ=25)


          ccccGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGG.....Gcagagcccccc                                                                                                 <  1:2206378/101‑11 (MQ=25)


                      tGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCCCGCACAGGTCGcc                                                                                     >  2:2160336/1‑101 (MQ=21)


                      tGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCCCGCACAGGTCGcc                                                                                     <  1:1908193/101‑1 (MQ=21)


                      tGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCCCGCACAGGTCGcc                                                                                     >  1:4465671/1‑101 (MQ=21)


                       gCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCCCGCACAGGTCGCCa                                                                                    >  1:3848788/1‑101 (MQ=21)


                       gCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCCCGCACAGGTCGCCa                                                                                    >  2:2159111/1‑101 (MQ=21)


                        cGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCCCGCACAGGTCGCCaa                                                                                   >  1:4227168/1‑101 (MQ=21)


                          gCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCCCGCACAGGTCGCCaaaa                                                                                 <  2:4497507/101‑1 (MQ=21)


                          gCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCCCGCACAGGTCGCCaaaa                                                                                 >  1:3951279/1‑101 (MQ=21)


                            aaGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCCCGCACAGGTCGCCAAAAgg                                                                               >  2:4635319/1‑101 (MQ=21)


                            aaGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCCCGCACAGGTCGCCAAAAgg                                                                               <  2:1790302/101‑1 (MQ=21)


                             aGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTCGCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGga                                                                              <  2:4448927/101‑1 (MQ=21)


                               aTGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGgacc                                                                            >  2:1816819/1‑101 (MQ=21)


                                tgtaGCCGATGTGCTTCTTCTTACGGCAAATATCCGGGGGGGAGGCTTGCTGATAGC.AAGACGGGGGGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGgaccg                                                                           <  2:772020/98‑1 (MQ=255)


                                 gAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGgaccga                                                                          >  2:3036764/1‑101 (MQ=21)


                                  aaGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGgaccgac                                                                         >  2:1201/1‑101 (MQ=21)


                                  aaGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGgaccgac                                                                         <  2:258401/101‑1 (MQ=21)


                                  aaGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGgaccgac                                                                         >  1:4235756/1‑101 (MQ=21)


                                  aaGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCCCGCACAGGCCGCCAAACGgaccgac                                                                         >  2:1888544/1‑101 (MQ=18)


                                   aGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGgaccgacc                                                                        <  1:1537906/101‑1 (MQ=21)


                                    gCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCCCGGACAGGTCGCCAAAAGGACCGACCt                                                                       >  2:1386654/1‑101 (MQ=18)


                                    gCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCt                                                                       <  1:2749404/101‑1 (MQ=21)


                                    gCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCt                                                                       >  1:241500/1‑101 (MQ=21)


                                    gCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGG.....GCAGAGACCCCCGCACAGGTCGCCAAAAGgaccgacat                                                                       >  1:1521024/1‑99 (MQ=21)


                                      cGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCa                                                                     <  1:4506164/101‑1 (MQ=21)


                                      cGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCa                                                                     >  1:100880/1‑101 (MQ=21)


                                       gATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCAg                                                                    >  1:3223216/1‑101 (MQ=21)


                                        aTGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGa                                                                   >  2:2700486/1‑101 (MQ=21)


                                        aTGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGa                                                                   >  2:1563419/1‑101 (MQ=21)


                                        aTGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGa                                                                   <  2:1406402/101‑1 (MQ=21)


                                         tGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGAt                                                                  >  2:3424927/1‑101 (MQ=21)


                                          gTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATc                                                                 <  1:782967/101‑1 (MQ=21)


                                           tCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCa                                                                <  1:4497985/101‑1 (MQ=21)


                                            ccTTCATCTTGCCGCAGATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAg                                                               >  1:4075084/1‑101 (MQ=25)


                                             cTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGc                                                              >  1:4389455/1‑101 (MQ=21)


                                              ttCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGcc                                                             >  2:3064905/1‑101 (MQ=21)


                                               tCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGccc                                                            >  1:3182280/1‑101 (MQ=21)


                                               tCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGccc                                                            >  1:766393/1‑101 (MQ=21)


                                                cATCTTGCCGCAAGTATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCg                                                           <  1:575846/101‑1 (MQ=25)


                                                  tCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGc                                                         >  2:4056323/1‑101 (MQ=21)


                                                     tGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCCCGCACAGGCCGCCAAAAGGACCGAACTCAGATCAGCCCGGCgtg                                                      >  2:523948/1‑101 (MQ=21)


                                                         gCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGc                                                  <  2:1137399/101‑1 (MQ=25)


                                                         gCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGc                                                  >  1:3106928/1‑101 (MQ=25)


                                                          cAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCa                                                 <  2:125412/101‑1 (MQ=255)


                                                           aaaTATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCAt                                                >  1:2987627/1‑101 (MQ=37)


                                                           aaaTATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCAt                                                >  2:3814545/1‑101 (MQ=37)


                                                            aaTATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATc                                               >  2:1368927/1‑101 (MQ=255)


                                                            aaTATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATc                                               >  2:1694189/1‑101 (MQ=255)


                                                            aaTATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATc                                               >  1:1313730/1‑101 (MQ=255)


                                                             atatCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATcg                                              >  2:3408732/1‑101 (MQ=255)


                                                              tatCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGGACCNACCTCAGATCAGCCCGGCGTGTTGCATcgc                                             >  1:661998/1‑101 (MQ=255)


                                                              tatCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATcgc                                             >  1:4292572/1‑101 (MQ=255)


                                                                 cccGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCgcggc                                          <  2:3520928/101‑1 (MQ=255)


                                                                 cccGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCgcggc                                          >  1:3364270/1‑101 (MQ=255)


                                                                   cGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGt                                        >  2:600178/1‑101 (MQ=255)


                                                                   cGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGt                                        >  2:4086725/1‑101 (MQ=255)


                                                                    ggggggAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTc                                       >  1:2616540/1‑101 (MQ=255)


                                                                    ggggggAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTc                                       >  2:4220298/1‑101 (MQ=255)


                                                                    ggggggAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTc                                       >  2:3346193/1‑101 (MQ=255)


                                                                    ggggggAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTc                                       >  2:2397515/1‑101 (MQ=255)


                                                                     gggggAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTCa                                      >  2:1001673/1‑101 (MQ=255)


                                                                      ggggAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCCCGCACAGGTCGCCACAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTCaa                                     >  2:563154/1‑101 (MQ=255)


                                                                      ggggAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTCaa                                     >  2:2269536/1‑101 (MQ=255)


                                                                      ggggAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTCaa                                     >  1:220476/1‑101 (MQ=255)


                                                                        ggAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCCTCGCGGCGTCAAgc                                   >  1:1019174/1‑101 (MQ=255)


                                                                         gAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTCAAgcg                                  >  1:2392474/1‑101 (MQ=255)


                                                                         gAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTCAAgcg                                  >  2:3147678/1‑101 (MQ=255)


                                                                         gAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTCAAgcg                                  <  2:1930439/101‑1 (MQ=255)


                                                                          aGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTCAAgcgc                                 <  1:206225/101‑1 (MQ=255)


                                                                          aGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTCAAgcgc                                 >  2:1304473/1‑101 (MQ=255)


                                                                           ggCTTGCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTCAAGcgcg                                <  2:4337392/101‑1 (MQ=255)


                                                                            gCTTGCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATAGCGGCGTCAAgagagg                               >  2:264864/1‑96 (MQ=255)


                                                                             cTTGCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCGGATCAGCCCGGCGTGTTGCATCGCGGCGTCAAGCGCGGc                              >  1:3571835/1‑101 (MQ=255)


                                                                             cTTGCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTCAAGCGCGGc                              >  1:609187/1‑101 (MQ=255)


                                                                                               ggggggcagagC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTCAAGCGCGGCCTTGGTCCCGTCGGTCa             >  1:4181139/11‑101 (MQ=255)


                                                                                               ggggggcagagC.....CCCCCGAACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTCAAGCGCGGCCTTGGTCCCGTCGGTCa             >  2:243763/11‑101 (MQ=255)


                                                                                                gggggcagagC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTCAAGCGCGGCCTTGGTCCCGTCGGTCAg            >  1:3608506/10‑101 (MQ=255)


                                                                                                 ggggcagagC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTCAAGCGCGGCCTTGGTCCCGTCGGTCAGt           <  1:4568843/93‑1 (MQ=255)


                                                                                                 ggggcagagC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTCAAGCGCGGCCTTGGTCCCGTCGGTCAGt           >  1:4170288/9‑101 (MQ=255)


                                                                                                    gcagagC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTCAAGCGCGGCCTTGGTCCCGTCGGTCAGTTCg        >  2:4049237/6‑101 (MQ=255)


                                                                                                    caccccc.....cccccGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTCAAGCGCGGCCTTGGTCCCGTCGGTCAGTTCg        <  1:2230245/95‑1 (MQ=255)


                                                                                                      agagC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTCAAGCGCGGCCTTGGTCCCGTCGGTCAGTTCGGt      >  1:2851247/4‑101 (MQ=255)


                                                                                                       gagC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTCAAGCGCGGCCTTGGTCCCGTCGGTCAGTTCGGTc     <  2:845709/99‑1 (MQ=255)


                                                                                                        agC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTCAAGCGCGGCCTTGGTCCCGTCGGTCAGTTCGGTCa    <  1:3134548/100‑1 (MQ=255)


                                                                                                         gC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTCAAGCGCGGCCTTGGTCCCGTCGGTCAGTTCGGTCAg   >  2:4312715/1‑101 (MQ=255)


                                                                                                          c.....cccccGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTCAAGCGCGGCCTTGGTCCCGTCGGTCAGTTCGGTCAGc  <  2:674092/101‑1 (MQ=255)


                                                                                                          c.....cccccGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTCAAGCGCGGCCTTGGTCCCGTCGGTCAGTTCGGTCAGc  >  1:2836318/1‑101 (MQ=255)


GCCCCCAGACCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGCAAAGACGGGGG.....GC.....CCCCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTCAAGCGCGGCCTTGGTCCCGTCGGTCAGTTCGGTCAGC  >  NC_014034/408398‑408599

Base quality scores:


ATCG

< 3 ≤


ATCG

< 20 ≤


ATCG

< 29 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG