BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	421,030	+G	1.8%	coding (443/828 nt)	RCAP_rcc00390 →	S49 family peptidase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	421,030	1	.→G	1.8%	2.0 ‑3.8 ‑0.1	168	coding (443/828 nt)	RCAP_rcc00390	S49 family peptidase
Reads supporting (aligned to +/- strand): new base (3/0): ref base (45/120): total (48/120)
Fisher's exact test strand distribution p-value = 2.23e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.12e-01

Only 100 of 226 total aligned reads displayed.


GATGCCACCTCGATCGTCGGCTCGATCGGGGTGATCTCCTCGGGCTTCGGCTTTGCCGATCTGATCGGCCGCTACGGCGTCGAGCGGCGGGTGCATACGGC.GGGC.GGTTC.GAAAAG.TTTCCTTGACCCGTTCCGACC.GGAAAAGCCCGAAGATGTGGACCGGC.TGACCCGGCTTCTGGAAGACATGCACCGCGCCTTCAAG  >  NC_014034/420930‑421130


gATGCCACCTCGATCGTCGGCTCGATCGGGGTGATCTCCTCGGGCTTCGGCTTTGCCGATCTGATCGGCCGCTACGGCGTCGAGCGGCGGGGGGATAcggc                                                                                                            >  1:3488377/1‑101 (MQ=255)


  tGCCACCTCGATCGTCGGCTCGATCGGGGTGATCTCCTCGGGCTTCGGCTTTGCCGATCTGATCGGCCGCTACGGCGTCGAGCGGCGGGTGCATAcggc.gg                                                                                                         <  2:1703849/101‑1 (MQ=255)


  tGCCACCTCGATCGTCGGCTCGATCGGGGTGATCTCCTCGGGCTTCGGCTTTGCCGATCTGATCGGCCGCTACGGCGTCGAGCGGCGGGTGCATAcggc.gg                                                                                                         <  2:2175805/101‑1 (MQ=255)


   gCCACCTCGATCGTCGGCTCGATCGGGGTGATCTCCTCGGGCTTCGGCTTTGCCGATCTGATCGGCCGCTACGGCGTCGAGCGGCGGGTGCATACggc.ggg                                                                                                        <  2:3509106/101‑1 (MQ=255)


   gCCACCTCGATCGTCGGCTCGATCGGGGTGATCTCCTCGGGCTTCGGCTTTGCCGATCTGATCGGCCGCTACGGCGTCGAGCGGCGGGGGCGTAAggg.ggg                                                                                                        >  2:1986814/1‑101 (MQ=255)


    ccACCTCGATCGTCGGCTCGATCGGGGTGATCTCCTCGGGCTTCGGCTTTGCCGATCTGATCGGCCGCTACGGCGTCGAGCGGCGGGTGCATACggc.gggc                                                                                                       <  2:1434822/101‑1 (MQ=255)


      aCCTCGATCGTCGTCTCGATCGGGGTGATCTCCTCGGGCTTCGGCTTTGCCGATTTGATCGGCCGCTACGGCGTCGAGCGGCGGGTGCATACGgc.gggc.gg                                                                                                    <  2:2295310/101‑1 (MQ=255)


       ccTCGATCGTCGGCTCGATCGGGGTGATCTCCTCGGGCTTCGGCTTTGCCGATCTGATCGGCCGCTACGGCGTCGAGCGGCGGGTGCATACGGC.GGGC.GGt                                                                                                   <  1:3861054/101‑1 (MQ=255)


         tCGATCGTCGGCTCGATCGGGGTGATCTCCTCGGGCTTCGGCTTTGCCGATCTGATCGGCCGCTACGGCGTCGAGCGGCGGGTGCATACGGC.GGGC.GGTTc                                                                                                 <  1:967736/101‑1 (MQ=255)


          cGATCGTCGGCTCGATCGGGGTGATCTCCTCGGGCTTCGGCTTTGCCGATCTGATCGGCCGCTACGGCGTCGAGCGGCGGGTGCATACGGC.GGGC.GGTTC.g                                                                                               <  2:1052808/101‑1 (MQ=255)


           gATCGTCGGCTCGATCGGGGTGATCTCCTCGGGCTTCGGCTTTGCCGATCTGATCGGCCGCTACGGCGTCGAGCGGCGGGGGGATACGgg.gggg.ggggtgg                                                                                               >  1:4060936/1‑96 (MQ=255)


              cgtcgGCTCGATCGGGGTGATCTCCTCGGGCTTCGGCTTTGCCGATCTGATCGGCCGCTACGGCGTCGAGCGGCGGGTGCATACGGC.GGGC.GGTTC.Gaaaa                                                                                           <  2:2095298/101‑1 (MQ=255)


                 cgGCTCGATCGGGGAGATCTCCTCGGGCTTCGGCTTTGCCGATCTGATCGGCCGCTACGGCGTCGAGCGGCGGGGGCCCACGGC.GGGC.GGGGC.Gaaaaagt                                                                                        >  1:620919/1‑98 (MQ=255)


                   gCTCGATCGGGGTGATCTCCTCGGGCTTCGGCTTTGCCGATCTGATCGGCCGCTACGGCGTCGAGCGGCGGGTGCATACGGC.GGGC.GGTTC.GAAAAG.TTTc                                                                                     <  2:3682798/101‑1 (MQ=255)


                      cGATCGGGGTGATCTCCTCGGGCTTCGGCTTTGCCGATCTGATCGGCCGCTACGGCGTCGAGCGGCGGGTGCATACGGC.GTGC.GGTTC.GAAAAG.TTTCCtt                                                                                  <  2:302811/101‑1 (MQ=255)


                      cGATCGGGGTGATCTCCTCGGGCTTCGGCTTTGCCGATCTGATCGGCCGCTACGGCGTCGAGCGGCGGGTGCATACGGC.GGGC.GGTTC.GAAAAG.TTTCCtt                                                                                  <  2:2730816/101‑1 (MQ=255)


                      cGATCGGGGTGATCTCCTCGGGCTTCGGCTTTGCCGATCTGATCGGCCGCTACGGCGTCGAGCGGCGGGTGCATACGGC.GGGC.GGTTC.GAAAAG.TTTCCtt                                                                                  <  1:864528/101‑1 (MQ=255)


                      cGATCGGGGTGATCTCCTCGGGCTTCGGCTTTGCCGATCTGATCGGCCGCTACGGCGTCGAGCGGCGGGGGCATACGGCGGGGG.GGTCC.GAAAAA.TTTCCt                                                                                   >  2:821945/1‑101 (MQ=255)


                        gtCGGGGTGATCTCCTCGGGCTTCGGCTTTGCCGAGCTGATCGGCCGCTACGGCGTCGAGCGGCGGGTGCATACGGC.GGGC.GGTTC.GAAAAG.TTTCCTTGa                                                                                <  2:521600/100‑1 (MQ=255)


                        aTCGGGGTGATCTCCTCGGGCTTCGGCTTTGCCGATCTGATCGGCCGCTACGGCGTCGAGCGGCGGGTGCATACGGC.GGGC.GGTTC.GAAAAG.TTTCCTTGa                                                                                <  1:450235/101‑1 (MQ=255)


                          cGGGGTGATCTCCTCGGGCTTCGGCTTTGCCGATCTGATCGGCCGCTACGGCGTCGAGCGGCGGGTGCATACGGC.GGGC.GGTTC.GAAAAG.TTTCCTTGAcc                                                                              <  1:1805065/101‑1 (MQ=255)


                           ggggTGATCTCCTCGGGCTTCGGCTTTGCCGATCTGATCGGCCGCTACGGCGTCGAGCGGCGGGTGCATACGGC.GGGC.GGTTC.GAAAAG.TTTCCTTGAccc                                                                             <  1:134269/101‑1 (MQ=255)


                            gggTGATCTCCTCGGGCTTCGGCTTTGCCGATCTGATCGGCCGCTACGGCGTCGAGCGGCGGGTGCATACGGC.GGGG.GGGTC.GGAAAG.GTTCCTTTACCCg                                                                            >  1:1058640/1‑101 (MQ=255)


                            gggTGATCTCCTCGGGCTTCGGCTTTGCCGATCTGATCGGCCGCTACGGCGTCGAGCGGCGGGTGCATACGGC.GGGC.GGTTC.GAAAAG.TTTCCTTGACCCg                                                                            <  1:1581543/101‑1 (MQ=255)


                              gTGATCTCCTCGGGCTTCGGCTTTGCCGATCTGATCGGCCGCTACGGCGTCGAGCGGCGGGGGGATACGGC.GGGG.GGGTCGGAAAAG.TTTCTtttcccctt                                                                           >  1:1021886/1‑94 (MQ=255)


                              gTGATCTCCTCGGGCTTCGGCTTTGCCGATCTGATCGGCCGCTACGGCGTCGAGCGGCGGGGGCATACGGC.GGGG.GGGTCGGAAAAA.TTTCTTTTCCCCGt                                                                           >  2:922488/1‑101 (MQ=255)


                                gATCTCCTCGGGCTTCGGCTTTGCCGATCTGATCGGCCGCTACGGCGTCGAGCGGCGGGTGCATACGGC.GGGC.GGTTC.GAAAAG.TTTCCTTGACCCGTTcc                                                                        <  2:1176616/101‑1 (MQ=255)


                                 aTCTCCTCGGGCTTCGGCTTTGCCGATCTGATCGGCCGCTACGGCGTCGAGCGGCGGGTGCATACGGC.GGGC.GGTTC.GAAAAG.TTTCCTTGACCCGTTCCg                                                                       <  1:778549/101‑1 (MQ=255)


                                 aTCTCCTCGGGCTTCGGCTTTGCCGATCTGATCGGCCGCTACGGCGTCGAGCGGCGGGTGCATACGGC.GGGC.GGTTC.GAAAAG.TTTCCTTGACCCGTTCCg                                                                       <  1:2690122/101‑1 (MQ=255)


                                 aTCTCCTCGGGCTTCGGCTTTGCCGATCTGATCGGCCGCTACGGCGTCGAGCGGCGGGGGCATCCGGC.GCGG.GGTTCTGAAAAG.TTTCTTTGACACGTGcc                                                                        >  2:708235/1‑101 (MQ=255)


                                  tctcCTCGGGCTTCGGCTTTGCCGATCTGATCGGCCGCTACGGCGTCGAGCGGCGGGTGCATACGGC.GGGC.GGTTC.GAAAAG.TTTCCTTGACCCGTTCCGa                                                                      <  2:3488119/101‑1 (MQ=255)


                                  tctcCTCGGGCTTCGGCTTTGCCGATCTGATCGGCCGCTACGGCGTCGAGCGGCGGGTGCATACGGC.GGGC.GGTTC.GAAAAG.TTTCCTTGACCCGTTCCGa                                                                      <  2:2633167/101‑1 (MQ=255)


                                    tcctcGGGCTTGGGCTTTGCCGATCTGATCGGCCGCTACGGCGTCGAGCGGCGGGTGCATACGGC.GGGC.GGTTC.GAAAAG.TTTCCTTGACCCGTTCCGAcc                                                                    <  2:1827018/101‑1 (MQ=255)


                                    tcctcGGGCTTCGGCTTTGCCGATCTGATCGGCCGCTACGGCGTCGAGCGGCGGGTGCATACGGC.GGGC.GGTTC.GAAAAG.TTTCCTTGACCCGTTCCGAcc                                                                    <  2:477228/101‑1 (MQ=255)


                                    tcctcGGGCTTCGGCTTTGCCGATCTGATCGGCCGCTACGGCGTCGAGCGGCGGGTGCATACGGC.GGGC.GGTTC.GAAAAG.TTTCCTTGACCCGTTCCGAcc                                                                    <  2:4629282/101‑1 (MQ=255)


                                    tcctcGGGCTTCGGCTTTGCCGATCTGATCGGCCGCTACGGCGTCGAGCGGCGGGTGCATACGGC.GGGC.GGTTC.GAAAAG.TTTCCTTGACCCGTTCCGAcc                                                                    <  1:4294874/101‑1 (MQ=255)


                                    tcctcGGGCTTCGGCTTTGCCGATCTGATCGGCCGCTACGGCGTCGAGCGGCGGGTGCATACGGC.GGGC.GGTTC.GAAAAG.TTTCCTTGACCCGTTCCGAcc                                                                    <  1:3270710/101‑1 (MQ=255)


                                    tcctcGGGCTTCGGCTTTGCCGATCTGATCGGCCGCTACGGCGTCGAGCGGCGGGGGCATTCGGC.GGGCGGGTCC.GAAAAG.TTCCCTTTCCCCGTTcgacc                                                                     >  2:2439585/1‑97 (MQ=255)


                                      ctcGGGCTTCGGCTTTGCCGATCTGATCGGCCGCTACGGCGTCGAGCGGCGGGTGCATACGGC.GGGC.GGTTC.GAAAAG.TTTCCTTGACCCGTTCCGACC.gg                                                                 <  2:3582661/101‑1 (MQ=255)


                                          ggCTTCGGCTTTGCCGATCTGATCGGCCGCTACGGCGTCGAGCGGCGGGTGCATACGGC.GGGC.GGTTC.GAAAAG.TTTCCTTGACCCGTTCCGACC.GGaaaa                                                             <  1:1225282/101‑1 (MQ=255)


                                          ggCTTCGGCTTTGCCGATCTGATCGGCCGCTACGGCGTCGAGCGGCGGGGGCATACGGC.GGGG.GGGTC.GAAAAG.ATTTCTTTACCCCTTCCGACC.CGgaaa                                                             >  1:4080953/1‑101 (MQ=255)


                                            cTTCGGCTTTGCCGATCTGATCGGCCGCTACGGCGTCGAGCGGCGGGGGCCTACGGC.GGGG.GGGTC.GAAAAA.GTTCCTTTACCCGCTCCCGCC.GGgaaaac                                                           >  2:2884640/1‑99 (MQ=255)


                                              tCGGCTTTGCCGATCTGATCGGCCGCTACGGCGTCGAGCGGCGGGTGCATACGGC.GGGC.GGTTC.GAAAAG.TTTCCTTGACCCGTTCCGACC.GGAAAAGccc                                                         <  2:570462/101‑1 (MQ=255)


                                              tCGGCTTTGCCGATCTGATCGGCCGCTACGGCGTCGAGCGGCGGGTGCATACGGC.GGGC.GGTTC.GAAAAG.TTTCCTTGACCCGTTCCGACC.GGAAAAGccc                                                         <  2:3661779/101‑1 (MQ=255)


                                               cGGCTTTGCCGATCTGATCGGCCGCTACGGCGTCGAGCGGCGGGTGCATACGGC.GGGC.GGTTC.GAAAAG.TTTCCTTGACCCGTTCCGACC.GGAAAAGCCCg                                                        <  1:2002737/101‑1 (MQ=255)


                                                ggCTTTGCCGATCTGATCGGCCGCTACGGCGTCGAGCGGCGGGTGCATACGGC.GGGC.GGTTC.GAAAAG.TTTCCTTGACCCGTTCCGACC.GGAAAAGCCCGa                                                       <  2:3256357/101‑1 (MQ=255)


                                                ggCTTTGCCGATCTGATCGGCCGCTACGGCGTCGAGCGGCGGGGGCATAAGGC.GGGG.GGGGC.GGAAAG.TTTCCTTTAACCGTTTCGACC.GGGAAAGCCCgc                                                       >  1:3750549/1‑100 (MQ=255)


                                                 gCTTTGCCGATCTGATCGGCCGCTACGGCGTCGAGCGGCGGGTGCATACGGC.GGGC.GGTTC.GAAAAG.TTTCCTTGACCCGTTCCGACC.GGAAAAGCCCGaa                                                      <  1:3071661/101‑1 (MQ=255)


                                                  cTTTGCCGATCTGATCGGCCGCTACGGCGTCGAGCGGCGGGTGCATACGGC.GGGC.GGTTC.GAAAAG.TTTCCTTGACCCGTTCCGACC.GGAAAAGCCCGAAg                                                     <  1:2276722/101‑1 (MQ=255)


                                                  cTTTGCCGATCTGATCGGCCGCTACGGCGTCGAGCGGCGGGGGCATACGGC.GGGG.GGGTC.GGAAAG.TTTCCCTTACCCGTTCCGACCGGGAAAAGccgcaa                                                      >  2:1770156/1‑97 (MQ=255)


                                                   tttGCCGATCTGATCGGCCGCTACGGCGTCGAGCGGCGGGGGCATACGGC.GGGG.GGGTC.GGAAAGTTTTCTTTGACCCGTCCCACCG.GGAAAACCCCGaaa                                                     >  2:1225064/1‑100 (MQ=255)


                                                   tttGCCGATCTGATCGGCCGCTACGGCGTCGAGCGGCGGGGGCATACGGC.GGGG.GGGTC.GAAAAG.TTTCCTTTACCCGTTCCGACC.CGAAAAACCCGAAGa                                                    >  1:4542307/1‑101 (MQ=255)


                                                    ttGCCGATCTGATCGGCCGCTACGGCGTCGAGCGGCGGGTGCATACGGC.GGGC.GGTTC.GAAAAG.TTTCCTTGACCCGTTCCGACC.GGAAAAGCCCGAAGAt                                                   <  2:643736/101‑1 (MQ=255)


                                                       ccGATCTGATCGGCCGCTACGGCGTCGAGCGGCGGGGGCATACGGC.GGGG.GGGTC.GAAAAA.GTTTCTTTACCCGTTTCCGCC.GGGAAAACCCGAAGaaggg                                                >  1:4374801/1‑97 (MQ=255)


                                                        cGATCTGATCGGCCGCTACGGCGTCGAGCGGCGGGTGCATACGGC.GGGC.GGTTC.GAAAAG.TTTCCTTGACCCGTTCCGACC.GGAAAAGCCCGAAGATGTgg                                               <  1:922568/101‑1 (MQ=255)


                                                          aTCTGATCGGCCGCTACGGCGTCGAGCGGCGGGTGCATACGGC.GGGC.GGTTC.GAAAAG.TTTCCTTGACCCGTTCCGACC.GGAAAAGCCCGAAGATGTGGAc                                             <  1:1702132/101‑1 (MQ=255)


                                                           tCTGATCGGCCGCTACGGCGTCGAGCGGCGGGTGCATACGGC.GGGC.GGTTC.GAAAAG.TTTCCTTGACCCGTTCCGACC.GGAAAAGCCCGAAGATGTGGAcc                                            <  1:822021/101‑1 (MQ=255)


                                                           tCTGATCGGCCGCTACGGCGTCGAGCGGCGGGTGCATACGGC.GGGC.GGTTC.GAAAAG.TTTCCTTGACCCGTTCCGACC.GGAAAAGCCCGAAGATGTGGAcc                                            <  2:1577628/101‑1 (MQ=255)


                                                            cTGATCGGCCGCTACGGCGTCGAGCGGCGGGTGCATACGGC.GGGC.GGTTC.GAAAAG.TTTCCTTGACCCGTTCCGACC.GGAAAAGCCCGAAGATGTGGACCg                                           <  1:2245583/101‑1 (MQ=255)


                                                             tGATCGGCCGCTACGGCGTCGAGCGGCGGGTGCATACGGC.GGGG.GGGTC.GAAAAG.GTTTCTTTACCCGTTCCGACC.GGAAAAGCCCCAAGATGTGGACCgg                                          >  1:577842/1‑101 (MQ=255)


                                                              gATCGGCCGCTACGGCGTCGAGCGGCGGGGGCATAAGGG.GGGG.GGGTC.GAAAAG.GTTCCTTACCCCGCTCCGGCC.GGGCAAGGCCGAAGAagtagggcagg                                         >  2:1532602/1‑93 (MQ=255)


                                                               aTCGGCCGCTACGGCGTCGAGCGGCGGGTGCATACGGC.GGGC.GGTTC.GAAAAG.TTTCCTTGACCCGTTCCGACC.GGAAAAGCCCGAAGATGTGGACCGGC.t                                       <  1:3963447/101‑1 (MQ=255)


                                                                tCGGCCGCTACGGCGTCGAGCGGCGGGTGCATACGGC.GGGC.GGTTC.GAAAAG.TTTCCTTGACCCGTTCCGACC.GGAAAAGCCCGAAGATGTGGACCGGC.Tg                                      <  2:2677431/101‑1 (MQ=255)


                                                                tCGGCCGCTACGGCGTCGAGCGGCGGGTGCATACGGC.GGGC.GGTTC.GAAAAG.TTTCCTTGACCCGTTCCGACC.GGAAAAGCCCGAAGATGTGGACCGGC.Tg                                      <  1:3446123/101‑1 (MQ=255)


                                                                tCGGCCGCTACGGCGTCGAGCGGCGGGGGCATACGGC.GGGG.GGGTC.GAAAAG.TTTTCTTTACCCCTTCCCACC.GGGAAAACCCCAAGAAGTGGGCCGGG.Tg                                      >  2:1638763/1‑101 (MQ=255)


                                                                  ggCCGCTACGGCGTCGAGCGGCGGGTGCATACGGC.GGGG.GGTTC.GAAAAG.TTTTCTTGACCCGTTCCGACC.GGAAAAACCCGAAGATGTGGGCCgggcgtc                                     >  2:3090952/1‑96 (MQ=255)


                                                                   gCCGCTACGGCGTCGAGCGGCGGGTGCATACGGC.GGGC.GGTTC.GAAAAG.TTTCCTTGACCCGTTCCGACC.GGAAAAGCCCGAAGATGTGGACCGGC.TGAcc                                   <  1:3197187/101‑1 (MQ=255)


                                                                   gCCGCTACGGCGTCGAGCGGCGGGGGCATACGGC.GGGC.GGGTC.GAAAAG.TTTCCTTTACCCGTTCCGACC.GGAAAAGCCCGAAGATGTGGGCCGGC.TGagc                                   >  1:1859666/1‑99 (MQ=255)


                                                                     cccTACGGCGTCGAGCGGCGGGTGCATACGGC.GGGC.GGTTC.GAAAAG.TTTCCTTGACCCGTTCCGCCC.GGAAAAGCCCGAAGATGTGGACCGGC.TGACCCg                                 <  2:2920738/99‑1 (MQ=255)


                                                                      gCTACGGCGTCGAGCGGCGGGTGCATACGGC.GGGC.GGTTC.GAAAAG.TTTCCTTGACCCGTTCCGACC.GGAAAAGCCCGAAGATGTGGACCGGC.TGACCCgg                                <  1:4400407/101‑1 (MQ=255)


                                                                      gCTACGGCGTCGAGCGGCGGGTGCATACCGG.GGGC.GGTTC.GAAAAG.TTTACCTGACCCGTTCCGACC.CGGAAAGGCCGAAGATGTGGAACGGG.TGAACCgg                                >  2:2855430/1‑101 (MQ=255)


                                                                       cTACGGCGTCGAGCGGCGGGTGCATACGGC.GGGC.GGTTC.GAAAAG.TTTCCTTGACCCGTTCCGACC.GGAAAAGCCCGAAGATGTGGACCGGC.TGACCCGGc                               <  2:3895607/101‑1 (MQ=255)


                                                                        tACGGCGTCGAGCGGCGGGTGCATACGGC.GGGC.GGTTC.GAAAAG.TTTCCTTGACCCGTTCCGACC.GGAAAAGCCCGAAGATGTGGACCGGC.TGACCCGGCt                              <  1:4656340/101‑1 (MQ=255)


                                                                        tACGGCGTCGAGCGGCGGGTGCATACGGC.GGGC.GGTTC.GAAAAG.TTTCCTTGACCCGTTCCGACC.GGAAAAGCCCGAAGATGTGGACCGGC.TGACCCGGCt                              <  1:3233094/101‑1 (MQ=255)


                                                                         aCGGCGTCGAGCGGCGGGGGCATACGGC.GGGC.GGGTC.GAAAAG.ATTCCTTTACCCCGTCCGACC.GGGAAAGCCCGCAGAAGTGGACCCGCGGGACCCGGCt                              >  2:432869/1‑101 (MQ=255)


                                                                          cGGCGTCGAGCGGCGGGTGCATACGGC.GGGC.GGTTC.GAAAAG.TTTCCTTGACCCGTTCCGACC.GGAAAAGCCCGAAGATGTGGACCGGC.TGACCCGGCTTc                            <  1:3339609/101‑1 (MQ=255)


                                                                           ggCGTCGAGCGGCGGGTGCATACGGC.GGGC.GGTTC.GAAAAG.TTTCCTTGACCCGTTCCGACC.GGAAAAGCCCGAAGATGTGGACCGGC.TGACCCGGCTTCt                           <  2:3835972/101‑1 (MQ=255)


                                                                            gCGTCGAGCGGCGGGTGCATACGGC.GGGC.GGTTC.GAAAAG.TTTCCTTGACCCGTTCCGACC.GGAAAAGCCCGAAGATGTGGACCGGC.TGACCCGGCTTCTg                          <  2:371199/101‑1 (MQ=255)


                                                                             cGTCGAGCGGCGGGTGCATACGGC.GGGC.GGTTC.GAAAAG.TTTCCTTGACCCGTTCCGACC.GGAAAAGCCCGAAGATGTGGACCGGC.TGACCCGGCTTCTgg                         <  1:2874420/101‑1 (MQ=255)


                                                                               tCGAGCGGCGGGGGCATACGGC.GGGG.GGGTC.GAAAAG.TTTCCTTTACCCGTTCCGACC.GGGAAAGCCCGAAGATGTGGACCGGC.TGGCCCGGCTTTTGGaa                       >  1:1096010/1‑101 (MQ=255)


                                                                                 gAGCGGCGGGTGCATACGGC.GGGC.GGTTC.GAAAAG.TTTCCTTTACCCGTTCCGACC.GGAAAAGCCCGAAGATGTGGACCGGC.TGACCCCGCTTCTAGAAGa                     >  2:3700202/1‑101 (MQ=255)


                                                                                  aGCGGCGGGTGCATACGGC.GGGC.GGTTC.GAAAAG.TTTCCTTGACCCGTTCCGACC.GGAAAAGCCCGAAGATGTGGACCGGC.TGACCCGGCTTCTGGAAGAc                    <  1:1880651/101‑1 (MQ=255)


                                                                                    cggcggGTGCATACGGC.GGGC.GGTTC.GAAAAG.TTTCCTTGACCCGTTCCGACC.GGAAAAGCCCGAAGATGTGGACCGGC.TGACCCGGCTTCTGGAAGACAt                  <  1:3319096/101‑1 (MQ=255)


                                                                                     ggcggGTGCATACGGC.GGGC.GGTTC.GAAAAG.TTTCCTTGACCCGTTCCGACC.GGAAAAGCCCGAAGATGTGGACCGGC.TGACCCGGCTTCTGGAAGACATg                 <  1:2439751/101‑1 (MQ=255)


                                                                                      gcggGTGCATACGGC.GGGC.GGTTC.GAAAAG.TTTCCTTGACCCGTTCCGACC.GGAAAAGCCCGAAGATGTGGACCGGC.TGACCCGGCTTCTGGAAGACATGc                <  1:1464141/101‑1 (MQ=255)


                                                                                        gggTGCATACGGC.GGGC.GGTTC.GAAAAG.TTTCCTTGACCCGTTCCGACC.GGAAAAGCCCGAAGATGTGGACCGGC.TGACCCGGCTTCTGGAAGACCTGCAc              >  1:149467/1‑101 (MQ=255)


                                                                                         ggTGCATACGGC.GGGC.GGTTC.GAAAAG.TTTCCTTGACCCGTTCCGACC.GGAAAAGCCCGAAGATGTGGACCGGC.TGACCCGGCTTCTGGAAGACATGCAcc             <  1:2084336/101‑1 (MQ=255)


                                                                                          gTGCATACGGC.GGGC.GGTTC.GAAAAG.TTTCCTTGACCCGTTCCGACC.GGAAAAGCCCGAAGATGTGGACCGGC.TGACCCGGCTTCTGGAAGAGATGCAccc            >  2:4377497/1‑100 (MQ=255)


                                                                                          gTGCATACGGC.GGGC.GGTTC.GAAAAG.TTTCCTTGACCCGTTCCGACC.GGAAAAGCCCGAAGATGTGGACCGGC.TGACCCGGCTTCTGGAAGACATGCACcg            >  2:3427543/1‑101 (MQ=255)


                                                                                          gTGCATACGGC.GGGC.GGTTC.GAAAAG.TTTCCTTGACCCGTTCCGACC.GGAAAAGCCCGAAGATGTGGACCGGC.TGACCCGGCTTCTGGAAGACATGCACcg            <  1:2161152/101‑1 (MQ=255)


                                                                                             gaTACCGC.GGGC.GGTTC.GAAAAG.TTTCCTTGACCCGTTCCGACC.GGAAAAGCCCGAAGATGTGGACCGGC.TGACCCGGCTTCTGGAAGACATGCACCgcgc         <  2:90234/100‑1 (MQ=255)


                                                                                             cATACGGC.GGGC.GGTTC.GAAAAG.TTTCCTTGACCCGTTCCGACC.GGAAAAGCCCGAAGATGTGGACCGGC.TGACCCGGCTTCTGGAAGACATGCACCgcgc         >  2:1822396/1‑101 (MQ=255)


                                                                                              aTACGGC.GGGC.GGTTC.GAAAAG.TTTCCTTGACCCGTTCCGACC.GGAAAAGCCCGAAGATGTGGACCGGC.TGACCCGGCTTCTGGAAGACATGCACCGCGcc        <  1:3677479/101‑1 (MQ=255)


                                                                                                aCGGC.GGGC.GGTTC.GAAAAG.TTTCCTTGACCCGTTCCGACC.GGAAAAGCCCGAAGATGTGGACCGGC.TGACCCGGCTTCTGGAAGACATGCACCGCGCCtt      <  2:4080667/101‑1 (MQ=255)


                                                                                                aCGGC.GGGC.GGTTC.GAAAAG.TTTCCTTGACCCGTTCCGACC.GGAAAAGCCCGAAGATGTGGACCGGC.TGACCCGGCTTCTGGAAGACATGCACCGCGCCtt      >  2:4514377/1‑101 (MQ=255)


                                                                                                 cggc.ggGC.GGTTC.GAAAAG.TTTCCTTGACCCGTTCCGACC.GGAAAAGCCCGAAGATGTGGACCGGC.TGACCCGGCTTCTGGGAGACATGCCCCGCGCCTTc     >  1:4613412/1‑101 (MQ=255)


                                                                                                 cggc.ggGC.GGTTC.GAAAAG.TTTCCTTGACCCGTTCCGACC.GGAAAAGCCCGAAGATGTGGACCGGC.TGACCCGGCTTCTGGAAGACATGCACCGCGCCTTc     <  2:2572266/101‑1 (MQ=255)


                                                                                                 cggc.ggGC.GGTTC.GAAAAG.TTTCCTTGACCCGTTCCGACC.GGAAAAGCCCGAAGATGTGGACCGGC.TGACCCGGCTTCTGGAAGACATGCACCGCGCCTTc     >  1:117094/1‑101 (MQ=255)


                                                                                                  ggc.gggc.gGTTC.GAAAAG.TTTCCTTGACCCGTTCCGACC.GGAAAAGCCCGAAGATGTGGACCGGC.TGACCCGGCTTCTGGAAGACATGCACCGCGCCTTCa    >  1:899218/1‑101 (MQ=255)


                                                                                                   gc.gggc.ggTTC.GAAAAG.TTTCCTTGACCCGTTCCGACC.GGAAAAGCCCGAAGATGTGGACCGGC.TGACCCGGCTTCTGGAAGACATGCACCGCGCCTTCaa   >  1:4184339/1‑101 (MQ=255)


GATGCCACCTCGATCGTCGGCTCGATCGGGGTGATCTCCTCGGGCTTCGGCTTTGCCGATCTGATCGGCCGCTACGGCGTCGAGCGGCGGGTGCATACGGC.GGGC.GGTTC.GAAAAG.TTTCCTTGACCCGTTCCGACC.GGAAAAGCCCGAAGATGTGGACCGGC.TGACCCGGCTTCTGGAAGACATGCACCGCGCCTTCAAG  >  NC_014034/420930‑421130

Base quality scores:


ATCG

< 3 ≤


ATCG

< 20 ≤


ATCG

< 29 ≤


ATCG

< 34 ≤


ATCG

< 41 ≤


ATCG