Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 432,425 +C 95.6% intergenic (+28/+120) RCAP_rcc00403 → / ← RCAP_rcc00404 hypothetical protein/Sua5/YciO/YrdC/YwlC family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034432,4251.→C95.6% 16.6 ‑3.9 ‑0.1180intergenic (+28/+120)RCAP_rcc00403/RCAP_rcc00404hypothetical protein/Sua5/YciO/YrdC/YwlC family protein
Reads supporting (aligned to +/- strand):  new base (56/116):  ref base (6/2):  total (62/118)
Fisher's exact test strand distribution p-value = 2.08e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.88e-01

Only 100 of 196 total aligned reads displayed.

AAATGGACCCGGGCGCTGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGT.CTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGC  >  NC_014034/432326‑432518
                                                                                                    |                                                                                                
aaaTGGACCCGGGCGCTGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGT.c                                                                                                 <  2:146925/101‑1 (MQ=255)
  aTGGGCCCGGGCGCTGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGT.cct                                                                                               <  2:2508135/101‑3 (MQ=255)
  aTGGACCCGGGCGCTGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGT.cct                                                                                               >  2:3269397/1‑99 (MQ=255)
    ggACCCGGGCGCTGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCTGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGT.cctgc                                                                                             >  2:660741/1‑97 (MQ=255)
       ccggggCGCTGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGa                                                                                           <  1:4517745/98‑1 (MQ=255)
        ccGGGCGCTGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGAc                                                                                          >  1:1745227/1‑101 (MQ=255)
         cGGGCGCTGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACt                                                                                         <  2:1505164/101‑1 (MQ=255)
         cGGGCGCTGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACt                                                                                         <  1:2901844/101‑1 (MQ=255)
         cGGGCGCTGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACt                                                                                         <  2:4131065/101‑1 (MQ=255)
         cGGGCGCTGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACt                                                                                         >  2:4540125/1‑101 (MQ=255)
         cGGGCGCTGGGCACGATGGGGATCGATCAGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACt                                                                                         <  2:3751558/101‑1 (MQ=255)
          gggCGCTGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTc                                                                                        >  1:801709/1‑101 (MQ=255)
            gcgcTGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTccc                                                                                      <  1:1862579/101‑1 (MQ=255)
             cgcTGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTcccc                                                                                     >  1:1712292/1‑101 (MQ=255)
             cgcTGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTcccc                                                                                     >  2:2542646/1‑101 (MQ=255)
              gcTGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTccccc                                                                                    <  2:1774051/101‑1 (MQ=255)
              gcTGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTccccc                                                                                    >  2:2583893/1‑101 (MQ=255)
               cTGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTcccccc                                                                                   <  1:3065446/101‑1 (MQ=255)
               cTGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTcccccc                                                                                   <  1:2054906/101‑1 (MQ=255)
                tGGGCACGGTGGGGATCTATCCGCGCACCCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCg                                                                                  <  2:596260/101‑1 (MQ=25)
                tGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCg                                                                                  <  1:3163389/101‑1 (MQ=255)
                 gggCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCgg                                                                                 <  2:3616489/101‑1 (MQ=255)
                  ggCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCggg                                                                                <  1:2219229/101‑1 (MQ=255)
                   gCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGa                                                                               <  1:1260421/101‑1 (MQ=255)
                    cACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGat                                                                              <  2:87104/101‑1 (MQ=255)
                     aCGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGGCCCCCGTCCTGCGGACTCCCCCCggggga                                                                             >  1:757883/1‑98 (MQ=37)
                     aCGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGat.a                                                                            <  1:1441799/101‑1 (MQ=255)
                      ctaTGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGACGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGat.at                                                                           <  1:3480016/99‑1 (MQ=255)
                       gATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCcgggaatt                                                                           >  1:943780/1‑96 (MQ=25)
                       gATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.Att                                                                          <  1:4064016/101‑1 (MQ=255)
                        aTGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.Attt                                                                         <  2:500052/101‑1 (MQ=255)
                        aTGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCCGGAG.Attt                                                                         >  1:2936190/1‑101 (MQ=25)
                         tGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTg                                                                        <  2:3547038/101‑1 (MQ=255)
                          ggggATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGCCTCCCCCCGGGAT.ATTTgg                                                                       >  1:2743295/1‑100 (MQ=255)
                          ggggATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGa                                                                       >  1:2824455/1‑101 (MQ=255)
                            ggggcgatcCGCGCCCCCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAgg                                                                     <  2:3859207/97‑1 (MQ=21)
                                cgatcCGCGCGCGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCTAg                                                                 <  1:2655095/101‑1 (MQ=25)
                                cgatcCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAg                                                                 <  2:931499/101‑1 (MQ=255)
                                cgatcCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAg                                                                 <  2:3129328/101‑1 (MQ=255)
                                cgatcCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAAGCAAg                                                                 >  2:1344295/1‑101 (MQ=255)
                                 gatcCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGa                                                                <  2:985326/101‑1 (MQ=255)
                                 gatcCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGa                                                                <  2:3716984/101‑1 (MQ=255)
                                 gatcCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGa                                                                <  1:340866/101‑1 (MQ=255)
                                 gagccGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGa                                                                <  1:4326705/98‑1 (MQ=255)
                                  ttgcgcgCACGCTTGCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCGGCCCCCCGTCCTGGGGACTCCCCCCGGGAT.ATTTGAGGCAAGAt                                                               <  1:1562112/98‑1 (MQ=21)
                                  atcCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGAt                                                               <  1:1047823/101‑1 (MQ=255)
                                     cgcgCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGaa                                                            <  2:4078281/101‑1 (MQ=255)
                                     cgcgCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGaa                                                            <  2:303400/101‑1 (MQ=255)
                                       cgcACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAg                                                          <  1:3930188/101‑1 (MQ=255)
                                       cgcACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAg                                                          <  1:2324509/101‑1 (MQ=255)
                                        gcACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGc                                                         >  2:533543/1‑101 (MQ=255)
                                          gcGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCg                                                       <  2:4442502/100‑1 (MQ=255)
                                          aCGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGGT.ATTTTTAGCAAGATGGAAggac                                                       >  2:833919/1‑98 (MQ=18)
                                          aCGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCg                                                       <  2:3375059/101‑1 (MQ=255)
                                          aCGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCg                                                       <  2:1598431/101‑1 (MQ=255)
                                          aCGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCg                                                       <  2:2707178/101‑1 (MQ=255)
                                             cTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATc                                                    <  2:618995/101‑1 (MQ=255)
                                             cTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATc                                                    <  2:793834/101‑1 (MQ=255)
                                             cTTGCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATc                                                    <  2:1974829/101‑1 (MQ=255)
                                               ttCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCg                                                  <  1:4309525/101‑1 (MQ=255)
                                               tgcgggggccgcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCg                                                  <  2:2015319/99‑1 (MQ=18)
                                                tCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCgg                                                 <  1:1487165/101‑1 (MQ=255)
                                                    cggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCtt                                             >  2:250936/1‑101 (MQ=255)
                                                     ggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTc                                            <  2:4309508/101‑1 (MQ=255)
                                                      gcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCg                                           <  1:3270444/101‑1 (MQ=255)
                                                       cggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGt                                          <  1:3670427/101‑1 (MQ=255)
                                                       cggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGt                                          >  2:2929000/1‑101 (MQ=255)
                                                       cggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTATGGCAAGATGAAAGGCCATCCCGGCTTCGt                                          >  1:273292/1‑101 (MQ=14)
                                                        ggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGGT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGtt                                         >  1:1629064/1‑101 (MQ=255)
                                                        ggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGGAAAATGAAAGACGGTCCggggcttggt                                         >  1:38039/1‑94 (MQ=17)
                                                            ggCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCAt                                     <  1:1053233/101‑1 (MQ=255)
                                                            ggCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCAt                                     <  2:1129175/101‑1 (MQ=255)
                                                              ccACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.t                                  <  2:1574779/101‑1 (MQ=255)
                                                               cACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.tt                                 <  1:3741999/101‑1 (MQ=255)
                                                                aCGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.g                               <  1:4666643/101‑1 (MQ=255)
                                                                 cGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.Gc                              <  1:4167960/101‑1 (MQ=255)
                                                                  gCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.Gcc                             <  1:68002/101‑1 (MQ=255)
                                                                     tGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCa                          <  1:3006872/101‑1 (MQ=255)
                                                                      gAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCaa                         >  1:161997/1‑101 (MQ=255)
                                                                      gAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCaa                         >  2:756285/1‑101 (MQ=255)
                                                                      gAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAAGCAAGATGAAAGTCGATACGGGCTTCGTTTCATC.TT.GCCCCaa                         >  2:32480/1‑101 (MQ=18)
                                                                       aGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGGTAATTTAAGGAAAAATAAAACCGGACCGGGGCTTCGTTT.ATCTTT.GCCCCaa                         >  2:3207103/1‑101 (MQ=2)
                                                                       aGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCaaa                        <  2:1736919/101‑1 (MQ=255)
                                                                         gCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAata                      <  1:4201899/101‑1 (MQ=255)
                                                                           cGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATc                    >  2:2813133/1‑101 (MQ=255)
                                                                            gggCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAG.ATTTGAGGGAAGATGAAAGCCGATCAGGGCTTCTTTTCATC.TT.CACCCAAAAATcc                   >  1:3011440/1‑101 (MQ=18)
                                                                             ggCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATccc                  <  2:351802/101‑1 (MQ=255)
                                                                              gacggggggCTCTGCCCCCCGG.CTTCG..CTCCCCCCGGGAT.ATTTGCGGCAAGATGAA....G....GGGCATGATTTCATC.TT.GCCCGAAATATCCCAGGGGTGAAttg      <  1:4617392/98‑2 (MQ=2)
                                                                              gacggggggCTCTGCCCCCCGG.CTTCG..CTCCCCCCGGGAT.ATTTGCGGCAAGATGAA....G....GGGCATGATTTCATC.TT.GCCCGAAATATCCCAGGGGTGAAttg      >  1:3063551/4‑100 (MQ=2)
                                                                              gCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCg                 <  1:19822/101‑1 (MQ=255)
                                                                               cAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCgg                >  2:4588357/1‑101 (MQ=255)
                                                                               acggggggCTCTGCCCCCCGG.CTTCG..CTCCCCCCGGGAT.ATTTGCGGCAAGATGAA....G....GGGCATGATTTCATC.TT.GCCCGAAATATCCCAGGGGTGAAttgg     >  1:2229861/3‑99 (MQ=2)
                                                                                aGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCGTCGTTTCATC.TT.GCCCCAAATATCCCggg               >  2:2142120/1‑101 (MQ=255)
                                                                                    gggCGCTGCCCCCCGTCCTGCGGCCTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTg           <  1:503068/101‑1 (MQ=255)
                                                                                     ggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGa          >  1:1890163/1‑101 (MQ=255)
                                                                                        gcTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAAtt       <  2:2827217/101‑1 (MQ=255)
                                                                                         cTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAAttt      <  2:2282586/101‑1 (MQ=255)
                                                                                           gCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTg    >  1:4387358/1‑101 (MQ=255)
                                                                                            ccccccGTCCTGCGGACCCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTgg   <  2:4189086/101‑1 (MQ=255)
                                                                                              ccccGTCCTGCGGACTCCCCCCGGGAT.ATTTTAGGGAAGAAGAAAACCGATCCCGGGCTCGTTTCATC.TTGGCCCAAAAAATCCCGGGGGGGAGTTTTgcg  >  2:3973020/1‑99 (MQ=12)
                                                                                                    |                                                                                                
AAATGGACCCGGGCGCTGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGT.CTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGC  >  NC_014034/432326‑432518

Base quality scores:  ATCG  < 3 ≤  ATCG  < 16 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG