Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 530,939 +G 2.3% intergenic (+17/+124) valS → / ← RCAP_rcc00497 valyl‑tRNA synthetase/UbiA prenyltransferase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034530,9392.→G 2.3% 2.3 ‑0.6 ‑0.2133intergenic (+17/+124)valS/RCAP_rcc00497valyl‑tRNA synthetase/UbiA prenyltransferase
Reads supporting (aligned to +/- strand):  new base (2/1):  ref base (53/77):  total (55/78)
Fisher's exact test strand distribution p-value = 5.69e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.06e-01

Only 100 of 159 total aligned reads displayed.

GAAGCCCGCGAAAACCTCGCCGCGCGCGCGGAAGAGGCGGCGAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTC.CCGCGCTTGTCGGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATCCCAA  >  NC_014034/530839‑531022
                                                                                                     |                                                                                   
gAAGCCCGCGAAAACCTCGCCGCGCGCGCGGAAGAGGCGGCGAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTc                                                                                      >  1:1501167/1‑101 (MQ=255)
    cccGCGAAAACCTCGCCGCGCGCGCGGAAGAGGCGGCGAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTctgcc                                                                                  <  2:934687/101‑5 (MQ=255)
     ccGCGAAAACCTCGCCGCGCGCGCGGAAGAGGCGGCGAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTctgccc                                                                                 <  2:830252/101‑6 (MQ=255)
       gcgAAAACCTCGCCGCGCGCGCGGAAGAGGCGGCGAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTctgccccc                                                                               <  1:433185/101‑8 (MQ=255)
       gcgAAAACCTCGCCGCGCGCGCGGAAGAGGCGGCGAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTctgccccc                                                                               <  1:2515840/101‑8 (MQ=255)
        cgAAAACCTCGCCGCGCGCGCGGAAGAGGCGGCGAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTctgcccccc                                                                              <  2:807698/101‑9 (MQ=255)
        cgAAAACCTCGCCGCGCGCGCGGAAGAGGCGGCGAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTctgcccccc                                                                              <  2:3202202/101‑9 (MQ=255)
        cgAAAACCTCGCCGCGCGCGCGGAAGAGGCGGCGAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTctgcccccc                                                                              >  1:2692377/1‑93 (MQ=255)
        cgAAAACCTCGCCGCGCGCGCGGAAGAGGCGGCGAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTctgcccccc                                                                              <  1:2558836/101‑9 (MQ=255)
        cgAAAACCTCGCCGCGCGCGCGGAAGAGGCGGCGAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTctgcccccc                                                                              <  1:2307068/101‑9 (MQ=255)
         gAAAACCTCGCCGCGCGCGCGGAAGAGGCGGCGAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTctgccccccg                                                                             <  1:1279386/101‑10 (MQ=255)
         gAAAACCTCGCCGCGCGCGCGGAAGAGGCGGCGAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTctgccccccg                                                                             >  1:103534/1‑92 (MQ=255)
                              gAAGAGGCGGCGAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATAtt                                                        <  2:4538467/101‑1 (MQ=25)
                              gAAGAGGCGGCGAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATAtt                                                        >  2:2863023/1‑101 (MQ=25)
                               aaGAGGCGGCGAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATAttt                                                       >  1:3068607/1‑101 (MQ=25)
                               aaGAGGCGGCGAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATAttt                                                       >  1:1177493/1‑101 (MQ=25)
                                     cggcgAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCa                                                 <  2:2387482/101‑1 (MQ=25)
                                     cggcgAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCTGCCCCCCGCTTCGCTCACCCCGGGATATTTTCGGCa                                                 >  2:2262490/1‑101 (MQ=25)
                                      ggcgAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCaa                                                >  1:2894617/1‑101 (MQ=25)
                                      ggcgAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGCATAAAGGAAGAAGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCaa                                                <  2:4624778/101‑1 (MQ=21)
                                       gcgAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGAAAAAGGAAGAAGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAg                                               <  1:1637307/101‑1 (MQ=21)
                                        cgAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGa                                              <  1:2611021/101‑1 (MQ=21)
                                        cgAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGa                                              <  2:4085854/101‑1 (MQ=21)
                                         gAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCTGCCCCCCGCTTCCCTCCCCCCGTGATATTTTCGGCAAGAt                                             >  2:1229801/1‑101 (MQ=21)
                                          aaGATGGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATa                                            <  2:1727916/101‑1 (MQ=21)
                                          aaGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATa                                            >  2:1506028/1‑101 (MQ=21)
                                          aaGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATa                                            >  1:4083430/1‑101 (MQ=21)
                                          aaGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATa                                            <  1:3335269/101‑1 (MQ=21)
                                          aaGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATa                                            >  1:4401618/1‑101 (MQ=21)
                                          aaGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATa                                            >  1:3253972/1‑101 (MQ=21)
                                           aGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATaa                                           <  2:219589/101‑1 (MQ=21)
                                            gATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATaaa                                          <  2:3627311/101‑1 (MQ=21)
                                             aTCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGTATATTTTCGGCAAGATAAAg                                         <  2:309875/101‑1 (MQ=21)
                                                ggcgcggcgcTGAAGCGTCTGGCCGACCTCGGATAAAGGAAGAGGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGcc                                      <  1:1569852/101‑1 (MQ=21)
                                                ggcgcggcgcTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGGGATTTTCTGCCAGATAAAgggc                                      >  2:2658836/1‑99 (MQ=16)
                                                ggcgcggcgcTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGcc                                      <  1:2641917/101‑1 (MQ=21)
                                                ggcgcggcgcTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGcc                                      <  2:4487128/101‑1 (MQ=21)
                                                ggcgcggcccTGAAGCGTCTGGCCGAACTCGGAGAAAGGAAGAAGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGcc                                      <  1:4027235/101‑1 (MQ=18)
                                                 gcgcggcgcTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGccc                                     <  2:1884102/101‑1 (MQ=21)
                                                 gcgcggcgcTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGccc                                     <  1:1158858/101‑1 (MQ=21)
                                                  cgcggcgcTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCg                                    <  1:1975556/101‑1 (MQ=21)
                                                   gcggcgcTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCgg                                   <  2:1317845/101‑1 (MQ=21)
                                                    cggcgcTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGa                                  >  2:3779141/1‑101 (MQ=21)
                                                     ggcgcTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGaa                                 >  1:3901464/1‑101 (MQ=21)
                                                     ggcgcTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGaa                                 <  2:144526/101‑1 (MQ=21)
                                                     ggcgcTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGaa                                 >  1:3934545/1‑101 (MQ=21)
                                                     ggcgcTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGaa                                 >  1:2412500/1‑101 (MQ=21)
                                                      gcgcTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCGCTGCCCCCCGCTTCGCTCCCCCCGGGAGATTTTCGGCAAGATAAAGGCCCGCAAg                                <  2:1197661/101‑1 (MQ=19)
                                                        gcTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGcc                              >  2:301012/1‑101 (MQ=21)
                                                         cTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCt                             <  2:742322/101‑1 (MQ=21)
                                                         cTGAAGCGTCTGGCCAAACTCGGATAAAGGAAGAAGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCt                             <  2:2071097/101‑1 (MQ=21)
                                                          tGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTc                            <  1:2497324/101‑1 (MQ=21)
                                                          tGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTc                            >  2:2117503/1‑101 (MQ=21)
                                                          tGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTc                            <  1:2982581/101‑1 (MQ=21)
                                                          tGAAGCATCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTc                            <  1:755867/101‑1 (MQ=21)
                                                           gAAGCGTCTGGCCGAACTCGGATAAAGGAAGAGGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTcc                           <  1:3217885/101‑1 (MQ=21)
                                                           gAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTcc                           <  1:3417262/101‑1 (MQ=21)
                                                           gAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTcc                           >  2:1455197/1‑101 (MQ=21)
                                                            aaGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTccc                          >  2:1642297/1‑101 (MQ=21)
                                                            aaGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTccc                          >  2:4214030/1‑101 (MQ=21)
                                                              gCGTCTGGCCGACCTCGGATAAAGGAAGAAGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCtt                        <  2:2129520/101‑1 (MQ=21)
                                                               cGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCttt                       <  2:3001441/101‑1 (MQ=21)
                                                               cGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCttt                       <  2:1015609/101‑1 (MQ=21)
                                                                gTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTa                      <  2:604527/101‑1 (MQ=21)
                                                                gTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTa                      <  2:229888/101‑1 (MQ=21)
                                                                 tCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTAt                     <  1:4508350/101‑1 (MQ=21)
                                                                  cTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATc                    >  1:3676508/1‑101 (MQ=21)
                                                                   tGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATCTTTTCGGCAAGATAAAGGCCCGGACGCCCCCCCTTTTCt                   >  1:4189929/1‑101 (MQ=16)
                                                                   tGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCt                   <  1:1124053/101‑1 (MQ=21)
                                                                    tctcGAACTCGGATAAAGGAAGAAGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCtt                  <  1:819352/98‑1 (MQ=21)
                                                                    ggCCGAACTCGGATAAAGGAAGAAGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCtt                  <  2:837614/101‑1 (MQ=21)
                                                                    ggCCGAACTCGGATAAAGGAAGAAGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCtt                  <  2:113222/101‑1 (MQ=21)
                                                                     gCCGAACTCGGATAAAGGAAGAAGGGGGGCTCTGCCCCCCTCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTg                 <  1:3847062/101‑1 (MQ=25)
                                                                     gCCGAACTCGGATAAAGGAAGAAGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTg                 <  2:2531622/101‑1 (MQ=21)
                                                                     gCCGAACTCGGATAAAGGAAGAAGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTg                 <  2:666417/101‑1 (MQ=21)
                                                                     gCCGAACTCGGATAAAGGAAGAAGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTg                 >  2:2735595/1‑101 (MQ=21)
                                                                     gCCGAACTCGGATAAAGGAAGAAGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTg                 <  1:4486101/101‑1 (MQ=21)
                                                                      ccGAACTCGGATAAAGGAAGAAGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGc                >  2:2678673/1‑101 (MQ=21)
                                                                       cGAACTCGGATAAAGGAAGAAGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGcc               <  1:614986/101‑1 (MQ=21)
                                                                        gAACTCGGATAAAGGAAGAAGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCg              >  1:4377828/1‑101 (MQ=21)
                                                                        gAACTCGGATAAAGGAAGAAGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCg              >  1:582375/1‑101 (MQ=21)
                                                                         aaCTCGGATAAAGGAAGAAGGGGGGCTCTGCCCCCCTCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCgg             <  1:239817/101‑1 (MQ=25)
                                                                         aaCTCGGATAAAGGAAGAAGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCgg             >  1:2743501/1‑101 (MQ=21)
                                                                          aCTCGGATAAAGGAAGAAGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGa            <  1:629815/101‑1 (MQ=21)
                                                                           cTCGGATAAAGGAAGAAGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGaa           >  1:1643138/1‑101 (MQ=21)
                                                                             cGGATAAAGGAAGAGGGGGGGCTCTGCCCCCCCCTTCGCT.CCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAata        <  2:4236658/101‑1 (MQ=19)
                                                                             cGGATAAAGGAAGAAGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAat         <  1:180299/101‑1 (MQ=21)
                                                                             cGGATAAAGGAAGAAGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAat         <  2:3472624/101‑1 (MQ=21)
                                                                              ggATAAAGGAAGAAGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAata        >  1:3464907/1‑101 (MQ=21)
                                                                               gATAAAGGAAGAAGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAatat       <  1:1747275/101‑1 (MQ=21)
                                                                                aTAAAGGAAGAAGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATTTc      <  1:2659021/101‑1 (MQ=21)
                                                                                aTAAAGGAAGAAGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATc      >  2:1571108/1‑101 (MQ=21)
                                                                                aTAAAGGAAGAAGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATc      >  2:4079216/1‑101 (MQ=21)
                                                                                aTAAAGGAAGAAGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATc      <  2:1266554/101‑1 (MQ=21)
                                                                                aTAAAGGAAGAAGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATc      <  2:2289833/101‑1 (MQ=21)
                                                                                 tAAAGGAAGAAGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATcc     <  2:2773846/101‑1 (MQ=21)
                                                                                 tAAAGGAAGAAGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATcc     <  1:1118151/101‑1 (MQ=21)
                                                                                  aaaGGAAGAAGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATccc    >  2:1500126/1‑101 (MQ=21)
                                                                                  aaaGGAAGAAGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATccc    <  1:1103256/101‑1 (MQ=21)
                                                                                   aaGGAAGAAGGGGGGCTCTGCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATCCCa   <  2:494592/101‑1 (MQ=21)
                                                                                                     |                                                                                   
GAAGCCCGCGAAAACCTCGCCGCGCGCGCGGAAGAGGCGGCGAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTC.CCGCGCTTGTCGGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATCCCAA  >  NC_014034/530839‑531022

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG