Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 568,801 +C 2.4% intergenic (‑97/+69) RCAP_rcc00530 ← / ← carB hypothetical protein/carbamoyl‑phosphate synthase large subunit

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034568,8011.→C 2.4% 5.3 0.0 ‑1.4167intergenic (‑97/+69)RCAP_rcc00530/carBhypothetical protein/carbamoyl‑phosphate synthase large subunit
Reads supporting (aligned to +/- strand):  new base (3/1):  ref base (98/65):  total (101/66)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.43e-02

Only 100 of 236 total aligned reads displayed.

TCATTCCTGCCTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGCCGGACCGAGATTTCGC  >  NC_014034/568701‑568901
                                                                                                     |                                                                                                    
tCATTCCTGCCTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTg                                                                                                       <  2:606889/101‑1 (MQ=255)
tCATTCCTGCCTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTg                                                                                                       <  1:3698783/101‑1 (MQ=255)
tCATTCCTGCCTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTg                                                                                                       <  1:1535623/101‑1 (MQ=255)
 cATTCCTGCCTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.c                                                                                                     >  1:1244789/1‑101 (MQ=255)
 cATTCCTGCCTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.c                                                                                                     >  1:388320/1‑101 (MQ=255)
  aTTCCTGCCTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.cc                                                                                                    <  2:2637047/101‑1 (MQ=255)
   ttCCTGCCTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.ccc                                                                                                   <  2:1707214/101‑1 (MQ=255)
   ttCCTGCCTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.ccc                                                                                                   >  2:1643810/1‑101 (MQ=255)
    tCCTGCCTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.cccc                                                                                                  <  1:2034599/101‑1 (MQ=255)
     ccTGCCTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.ccccc                                                                                                 >  2:4593449/1‑101 (MQ=255)
     ccTGCCTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGGGAAGGCGCTGCGGCATCCCG.ccccc                                                                                                 >  1:585714/1‑101 (MQ=255)
      cTGCCTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.cccccc                                                                                                >  2:133854/1‑101 (MQ=255)
       tGCCTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.ccccccc                                                                                               <  1:1653871/101‑1 (MQ=255)
       tGCCTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.ccccccc                                                                                               <  2:3101183/101‑1 (MQ=255)
        gCCTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.cccccccc                                                                                              <  1:4207047/101‑1 (MQ=255)
        gCCTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.cccccccc                                                                                              <  1:305542/101‑1 (MQ=255)
           tCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCg                                                                                           <  1:519145/101‑1 (MQ=255)
             cAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGAc                                                                                         >  2:2505492/1‑101 (MQ=255)
                tttCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGGGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCACCCTG.CCCCCCCCCCgcccgg                                                                                      >  2:3391615/1‑96 (MQ=255)
                tttCTGTAGGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGCCCCGCACGGGCTTGGGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGACggg                                                                                      <  2:3549002/101‑1 (MQ=255)
                 ttCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGCCGGGa                                                                                     >  1:1179113/1‑101 (MQ=255)
                  tCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGACGGGag                                                                                    >  1:2918351/1‑101 (MQ=255)
                  tCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGACGGGag                                                                                    >  2:3790331/1‑101 (MQ=255)
                   cTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGACGGGggg                                                                                   >  2:2146191/1‑101 (MQ=255)
                   cTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGGGGGGCAATGACCCGCACGGGGTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGACGGGagg                                                                                   <  1:1960975/101‑1 (MQ=255)
                     gTATGTGAACCGGGGCTTACGCCTGGGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGACGGGagggc                                                                                 <  2:4559665/101‑1 (MQ=255)
                      tATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGACGGGagggcg                                                                                >  1:2986536/1‑100 (MQ=255)
                      tATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGACGGGagggca                                                                                <  1:1464221/101‑1 (MQ=255)
                       tttggAACCGGGGCTTACGCCTGGGGCTGGGGGGGGGCAATGCCCCGCACGGGCTTTTGAAGGCGCTGCGGCATCCTGCCCCCCCCCCCGACGGGagggca                                                                                <  2:2340918/97‑1 (MQ=255)
                        tgtgAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCCACGGGgggggcgg                                                                              >  2:2753754/1‑97 (MQ=255)
                          tgAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGACGGGggggccgggc                                                                            >  1:1362662/1‑101 (MQ=255)
                           gAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGACGGGAGGGCAGGGCt                                                                           <  2:2947379/101‑1 (MQ=255)
                           gAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG..CCCCCCCCCGACGGGAGGGCAGGGCTg                                                                          >  2:3368930/1‑101 (MQ=255)
                               cGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGACGGGAGGGCAGGGCTGGtt                                                                       >  1:417948/1‑101 (MQ=255)
                                ggggCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGACGGGAGGGCAGGGCTGGttt                                                                      <  2:417911/101‑1 (MQ=255)
                                ggggCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCCGCGGGGGGGCCGGGCTGGgtt                                                                      >  1:1945821/1‑101 (MQ=255)
                                 gggCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGACGGGGGGGCAGGGCTGGGTTc                                                                     >  2:4329633/1‑101 (MQ=255)
                                 gggCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTc                                                                     >  1:2224864/1‑101 (MQ=255)
                                 gggCTTACGCCTGTGGCTGCGGGGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTc                                                                     <  2:1215009/101‑1 (MQ=255)
                                  ggCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGACGGGGGGGCAGGGCTGGTTTCg                                                                    >  1:3869684/1‑101 (MQ=255)
                                    cTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGACGGGGGGGCAGGGCTGGGTTCggg                                                                  >  1:4564765/1‑101 (MQ=255)
                                      tACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCCGCCGGGGGGCCGGGCCGGGTTTggggg                                                                >  2:3498374/1‑99 (MQ=255)
                                      tACGCCTGTGGCTGCGGGGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGggcg                                                                <  2:1967261/101‑1 (MQ=255)
                                       aCGCCTGTGGCTGCGGTGGGGCAATGCCCCGCACGGGCTTGTGAAGGCGCTGCGGCCTCCTG.CCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGggcgg                                                               <  1:1159158/101‑1 (MQ=255)
                                       aCGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGCCGGGGGGGCAGGGCTGGTTTCGggcgg                                                               >  1:3729/1‑101 (MQ=255)
                                         gCCTGTGGCGGCGGGGGGGCAATGACCCGCCCGGGCTTGGGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCa                                                             <  1:1483599/101‑1 (MQ=255)
                                          ccTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGACGGGGGGGCAGGGCTGGTTTGGGGGGGCaa                                                            >  1:2733672/1‑101 (MQ=255)
                                           cTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGACGGGGGGGCAGGGCTGGTTTCGGGCGGCAAg                                                           >  2:2801584/1‑101 (MQ=255)
                                           cTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGGGGCAAg                                                           >  2:1876898/1‑101 (MQ=255)
                                            tgtgGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGACGGGGGGGCGGGGCGGGTTTCGGGCGGCAAgg                                                          >  2:1486895/1‑101 (MQ=255)
                                             gtgGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTGCCCCCCCCCCCGACGGGGGGGCAGGGGTGGTTTGGGGCGGCagag                                                          >  2:325296/1‑98 (MQ=255)
                                               ggCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGACGGGAGGGGAGGGCGGGTTTCGgggggggaggggc                                                       >  1:4299951/1‑93 (MQ=255)
                                                gCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGcc                                                      >  1:3166278/1‑101 (MQ=255)
                                                  tGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGGGGCAAGGGGCCcg                                                    >  1:878186/1‑101 (MQ=255)
                                                  gggggTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCcg                                                    <  2:1666695/98‑1 (MQ=255)
                                                   ggggTGGGGGAATGGCCCGGACGGGGTTGGGAAGGGCGTGGCGCCTCCTG.CCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCcgc                                                   <  1:1883501/99‑1 (MQ=255)
                                                   gCGGTGGGGCAATGACCCGCCCGGGCTTGTGAAGGCGCTGCGGCATCCTGCCCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCcg                                                    <  1:2554743/101‑1 (MQ=255)
                                                   gCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCcgc                                                   <  1:2484670/101‑1 (MQ=255)
                                                       tGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCcgcggcg                                               <  1:3568916/101‑1 (MQ=255)
                                                            cAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGGGCGGcc                                          >  1:3394727/1‑101 (MQ=255)
                                                             ccttgcccGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGACGGGGGGGCAGGGCCGGGTTCGGGGGGGAAAGCCCCcgccgcgcgggcc                                         >  2:650630/6‑98 (MQ=255)
                                                             aaTGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG..CCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGGAAGGCGCCCGCGGCGCGGCCgg                                        >  2:3735190/1‑100 (MQ=255)
                                                                gACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGCCGGGGGGGCCGGGGTGGGTTCCGGCGGGAAGGCGCCCCCCGCGCCGccccgc                                      >  2:98025/1‑97 (MQ=255)
                                                                  cccGCACGGGCTTGGGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGcat                                    <  1:920138/101‑3 (MQ=255)
                                                                    cGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGACGGGGGGGCAGGGCTGGTTTCGGGGGGTCAGGCGCCCGCGGCGCGGGCGGGccccc                                  >  1:436901/1‑98 (MQ=255)
                                                                     gCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGGGGCAAAGCGCCCGCGGGGCGGGCGGGCCCGCt                                 >  2:526398/1‑101 (MQ=255)
                                                                     gCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGGGCCTGCt                                 >  2:3193610/1‑101 (MQ=255)
                                                                     gCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCCCGGGCCGGCCGGGCCTGCt                                 >  2:1078655/1‑101 (MQ=255)
                                                                     ccagggccTTGGGAAGGCCCGGCGCCACCCTC.CCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCt                                 <  2:4516622/94‑1 (MQ=255)
                                                                      cACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCtt                                <  2:1958360/101‑1 (MQ=255)
                                                                      cACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCtt                                <  1:65189/101‑1 (MQ=255)
                                                                       gggggCTTGGTGAGGCCCTGCCGCAACCTG.CCCCCCCCCCGGCGGGGGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTa                               <  2:2847901/99‑1 (MQ=255)
                                                                       ccGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTa                               <  2:4034207/100‑1 (MQ=255)
                                                                         gggCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGGGCCTGCTTACg                             >  2:217542/1‑101 (MQ=255)
                                                                          ggCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGGCGGGGGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGGGCCCGCtttccc                            >  2:396458/1‑97 (MQ=255)
                                                                           gCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGcc                           <  1:2600727/101‑1 (MQ=255)
                                                                            cTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGACGGGAGGCCAGGGCTGGTTTCGGGCGGCAAGGGGCCCGCGGCGCGGCCGCGCCTGCTTACGccc                          >  1:4632181/1‑100 (MQ=255)
                                                                            cTTGTGAAGGCGCTGCGGCATCCTG..CCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTg                         >  2:2961445/1‑101 (MQ=255)
                                                                             ttGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCGGCTTTCGcccg                         >  2:3436304/1‑99 (MQ=255)
                                                                             ttGGGAGGGGGCTGCGGCCCCTTG.CCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTg                         <  2:679557/101‑1 (MQ=255)
                                                                              tttgAAGGCCCTGCCGCCTCCCGCCCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTg                         <  1:325328/99‑1 (MQ=255)
                                                                              tgtgAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGc                        <  1:3182084/101‑1 (MQ=255)
                                                                              ggggAAGGCGGTGGGCCCCCCTG.CCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGc                        <  1:838148/98‑1 (MQ=255)
                                                                               gtgAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGACGGGGGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGGGCCCGCTTACGCCCGcc                       >  1:3553099/1‑100 (MQ=255)
                                                                                tgAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCCGCTTACCCCTGCAg                      >  1:4121233/1‑101 (MQ=255)
                                                                                   aGGCGCTGCGGCACCCCG.CCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCgg                   <  1:2663649/101‑1 (MQ=255)
                                                                                    ggCGCTGCGGCATCCTG.CCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGc                  <  2:2566588/101‑1 (MQ=255)
                                                                                    ggCGCTGCGGCATCCTG.CCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGc                  <  1:2801800/101‑1 (MQ=255)
                                                                                     gcgcTGCGGCATCCTG.CCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGcc                 <  1:717328/101‑1 (MQ=255)
                                                                                      cggtGCGGCCTCCCG.CCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGCCg                <  1:3345023/98‑1 (MQ=255)
                                                                                       gcTGCGGCATCCTG.CCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCCGCAGCGGCCgg               >  1:4542687/1‑101 (MQ=255)
                                                                                       gcTGCGGCATCCTG..CCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCCGCGGCCggg              >  1:2674774/1‑100 (MQ=255)
                                                                                          gctgCATCCTG..CCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGCCGGACCg           >  1:3832164/4‑101 (MQ=255)
                                                                                            ggCATCCTG.CCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGCCGGCCCgg          >  1:3763542/1‑100 (MQ=255)
                                                                                            ggCACCCTG.CCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGCCGGACCga          <  1:46970/101‑1 (MQ=255)
                                                                                               ccccTG.CCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGCCGGACCGAGAt       <  2:3166034/99‑1 (MQ=255)
                                                                                               aTCCTG.CCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGGCGGGCCCGCTTCCGCCCGCAGCGGCCGGGCCGGGAt       >  1:1705595/1‑101 (MQ=255)
                                                                                               aTCCTG.CCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGCCGGACCGAGAt       >  2:371145/1‑101 (MQ=255)
                                                                                                 ccTG.CCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGCCGGACCGAGAttt     <  2:1154120/101‑1 (MQ=255)
                                                                                                  cTG.CCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGGGCCCGCTTACGCCTGCAGCGGCCCGCCCgcggcttc    >  2:3930404/1‑94 (MQ=255)
                                                                                                     |                                                                                                    
TCATTCCTGCCTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGCCGGACCGAGATTTCGC  >  NC_014034/568701‑568901

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 23 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG