Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	900,802	+CC	100%	intergenic (‑131/‑156)	ldh ← / → ftsW	L‑lactate dehydrogenase/cell division protein FtsW

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	900,802	1	.→C	100.0%	100.0	43	intergenic (‑131/‑156)	ldh/ftsW	L‑lactate dehydrogenase/cell division protein FtsW
Reads supporting (aligned to +/- strand):  new base (32/10):  ref base (0/1):  total (32/11)
*	NC_014034	900,802	2	.→C	100.0%	102.4	44	intergenic (‑131/‑156)	ldh/ftsW	L‑lactate dehydrogenase/cell division protein FtsW
Reads supporting (aligned to +/- strand):  new base (32/11):  ref base (0/1):  total (32/12)

AACCCCGTTTCCG.CGCGG.CGCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGTGC...C.GCGGA..G..CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGCAAAAGCTCTGGTCAGAGG  >  NC_014034/900725‑900876                                                                                          ||                                                                           aaCCCCGTTTCCGCCGCGGCCGCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGTGCGGGG.GCGGA..GCCCCCGCGGGTCGCCCCg                                                            >  2:3722259/1‑101 (MQ=16)   ccccGTTTCCGCCGCGGCCGCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGTGCGGGG.GCGGA..GCCCCCGCGGGTCGCCCCGGc                                                          >  2:3852406/1‑101 (MQ=16)    cccGTTTCCGCCGCGGCCGCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGTGCGGGG.GCGGA..GCCCCCGCGGGTCGCCCCGGcc                                                         >  1:4624299/1‑101 (MQ=16)      cGTTTCCGCCGCGGCCGCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGTGCGGGG.GCGGA..GCCCCCGCGGGTCGCCCCGGCCCt                                                       <  2:2131546/101‑1 (MQ=16)      cGTTTCCGCCGCGGCCGCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGTGCGGGG.GCGGA..GCCCCCGCGGGTCGCCCCGGCCCt                                                       >  1:75730/1‑101 (MQ=16)       gttcccgccgcgGCCGCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGTGCGGGG.GCGGA..GCCCCCGCGGGTCGCCCCGGCCCTg                                                      <  2:3633764/93‑1 (MQ=16)           ccgcggcggccgccgcTTGGCCGCACCCCCGGTTTTCCTTGGTGCAAATCCGCC...CGGGGGGGCGGGG.GCGGA..GCCCCCGCGGGTCGCCCCGGCCCTGCCgg                                                  <  2:403654/91‑1 (MQ=12)           ccgccgcgGCCGCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGTGCGG.CGGCGGA..GCCCCCGCGGGTCGCCCCGGCCCTGCCgg                                                  <  2:1790314/97‑1 (MQ=21)              ccccggccgccgcTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCC...GGGGGTTGCGGGG.GCGGA..GCCCCCGCGGGTCGCCCCGGCCCTGCCGGGGc                                               <  2:3137130/94‑1 (MQ=12)                gcggccgccgcTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGTGCGGGG.GCGGA..GCCCCCGCGGGTCGCCCCGGCCCTGCCGGGGCGa                                             >  2:2834885/6‑101 (MQ=18)                ccgtcctccgcTTTGCCGCCGCCCCGGTTTTCTGTGGAGGAAACCCGCC...GGGGGGGGCGGGG.GCGGG..GCCCCCGCGGGTCGCCCCGGCCCTGCCGGGGCGa                                             <  1:660209/94‑1 (MQ=2)                  ggccgccgcTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGTGCGGGG.GCGGA..GCCCCCGCGGGTCGCCCCGGCCCTGCCGGGGCGaaa                                           >  2:2901134/4‑101 (MQ=18)                       ccgcTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGTGCGGGG.GCGGA..GCCCCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGcccc                                      >  1:2711427/1‑101 (MQ=21)                        cgcTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGTGCGGGG.GCGGA..GCCCCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGccccc                                     >  1:3084185/1‑101 (MQ=18)                           tttGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGTGCGGGG.GCGGA..GCCCCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCcgcg                                  >  2:2648011/1‑101 (MQ=18)                            ttGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGTGCGGGG.GCGGA..GCCCCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCcgcgc                                 >  2:702966/1‑101 (MQ=18)                             tGCCGCAGCCCGGGTTTTCCTTGGTGCAAATACGCC...CGGGGGGGCGGGG.GCGGA..GCCCCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCcgcgcg                                <  2:4410957/101‑1 (MQ=18)                             tGCCGCAGCCCCGGTTTGCCTTGGTGCAAAGACGCC...CGGGGGTGCGGGG.GCGGA..GCCCCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCcgcgcg                                <  2:2113322/101‑1 (MQ=18)                              gCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGTGCGGGG.GCGGA..GCCCCCGCGGGTCGCCCCGGCCCTGCCGGGGGGAAAGCCCCCgcgcgc                               >  2:2128691/1‑101 (MQ=18)                              gCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCC...AGGGGGGGCGGGG.GCGGA..GCCCCCGCGGGCAGCCCCGGCCCTGCCGGGGCGACAGCCCCCgcgcgc                               >  2:3132905/1‑101 (MQ=16)                                cGCAGCCCCGTTTTTCCTTGGTGAAAATGCGCCGGGGGGGGGGGG...G.GCGGACCC..CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGcgccg                             <  1:4663680/101‑1 (MQ=2)                                 gCAGCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGTGCGGGG.GCGGA..GCCCCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGcgccgc                            >  2:3550534/1‑101 (MQ=18)                                 gCAGCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGTGCGGGG.GCGGA..GCCCCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGcgccgc                            >  1:1864200/1‑101 (MQ=18)                                 gCAGCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGTGCGGGG.GCGGA..GCCCCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGcgccgc                            >  1:1340947/1‑101 (MQ=18)                                  cAGCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGTGCGGGG.GCGGA..GCCCCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCcgcg                           >  1:4099414/1‑101 (MQ=18)                                   aGCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGTGCGGGG.GCGGA..GCCCCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCgg                          >  1:2226899/1‑101 (MQ=18)                                   aGCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGTGCGGGG.GCGAA..GCCCCCGCGGGTCGCCCCCGCCCTGCCGCGGCGAAACCCCCCGGGCGCCGCgg                          >  2:3182491/1‑101 (MQ=12)                                    gCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGTGCGGGG.GCGGA..GCCCCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGc                         >  1:1956741/1‑101 (MQ=18)                                    gCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGTGCGGGG.GCGGA..GCCCCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGc                         >  2:3040520/1‑101 (MQ=18)                                       ccGGTTTTCCTTGGTGCAAATACGCC...CGGGGGTGCGGGG.GCGGA..GCCCCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCg                      >  1:3231048/1‑101 (MQ=18)                                         ggTTTTCCTTGGTGCAAATACGCC...CGGGGGTGCGGGG.GCGGA..GCCCCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGc                    >  2:3787500/1‑101 (MQ=18)                                          gTTTTCCTTGGTGCAAATACGCC...CGGGGGTGCGGGG.GCGGA..GCCCCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGCa                   >  2:2972276/1‑101 (MQ=18)                                          gTTTTCCTTGGTGCAAATACGCC...CGGGGGTGCGGGG.GCGGA..GCCCCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGCa                   >  1:1227844/1‑101 (MQ=18)                                           ttttCCTTGGTGCAAATACGCC...CGGGGGTGCGGGG.GCGGA..GCCCCCGCGGGTTGCCCCGGCCCTGCCGGGGCGACAGCCCCCGCGCGCCGCGGCCCGGcca                  >  2:917310/1‑99 (MQ=21)                                           ttttCCTTGGTGCAAATACGCC...CGGGGGTGCGGGG.GCGGA..GCCCCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGCaa                  >  2:570832/1‑101 (MQ=18)                                            tttCCTTGGTGCAAATACGCC...CGGGGGTGCGGGG.GCGGA..GCCCCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGCaaa                 >  2:4073816/1‑101 (MQ=18)                                              tCCTTGGTGCAAATACGCC...CGGGGGTGCGGGG.GCGGA..GCCCCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGCAAAAg               >  2:3647753/1‑101 (MQ=18)                                              tCCTTGGTGCAAATACGCC...CGGGGGTGCGGGG.GCGGA..GCCCCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGCAAAAg               >  2:1784825/1‑101 (MQ=18)                                                 ttGGTGCAAATACGCC...CGGGGGTGCGGGG.GCGGA..GCCCCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGCAAAAGctc            <  2:2711231/101‑1 (MQ=18)                                                  tGGTGCAAATCCGCC...CGGGGGTGCGGGG.GCGGA..GCCCCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGCAAAAGctct           <  1:2857270/101‑1 (MQ=18)                                                  tGGTGCAAATACGCC...CGGGGGTGCGGGG.GCGGA..GCCCCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGCAAAAGctct           >  1:2017187/1‑101 (MQ=18)                                                     tGCAAATACGCC...CGGGGGTGCGGGG.GCGGA..GCCCCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGCAAAAGCTCTGGt        <  1:3830194/101‑1 (MQ=18)                                                          aTACGCC...CGGGGGTGCGGGG.GCGGA..GCCCCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGCAAAAGCTCTGGTCagag   >  2:2712419/1‑101 (MQ=18)                                                          aTACGCC...CGGGGGTGCGGGG.GCGGA..GCCCCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGCAAAAGCTCTGGTCagag   <  2:1509957/101‑1 (MQ=18)                                                           tACGCC...CGGGGGTGCGGGG.GCGGA..GCCCCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGCAAAAGatcggaagagcgt  >  2:1949592/1‑91 (MQ=12)                                                                                          ||                                                                           AACCCCGTTTCCG.CGCGG.CGCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGTGC...C.GCGGA..G..CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGCAAAAGCTCTGGTCAGAGG  >  NC_014034/900725‑900876 Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG