Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,303,885 C→T 4.0% intergenic (+72/+85) urtE → / ← RCAP_rcc01231 urea ABC transporter ATP‑binding protein UrtE/UbiH/UbiF/VisC/COQ6 family ubiquinone biosynthesis hydroxylase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,303,8850C→T 4.0% 3.3 ‑3.2 ‑0.0100intergenic (+72/+85)urtE/RCAP_rcc01231urea ABC transporter ATP‑binding protein UrtE/UbiH/UbiF/VisC/COQ6 family ubiquinone biosynthesis hydroxylase
Reads supporting (aligned to +/- strand):  new base (4/0):  ref base (42/54):  total (46/54)
Fisher's exact test strand distribution p-value = 4.16e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.70e-01

Only 100 of 114 total aligned reads displayed.

ACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCT.C..CGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCC....GCCAAGATGGGG..G..GC.CCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCAT  >  NC_014034/1303787‑1303978
                                                                                                        |                                                                                                      
aCCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCAc                                                                                                        <  2:350269/101‑1 (MQ=37)
 cccTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCAcc                                                                                                       >  2:762776/1‑101 (MQ=37)
 cccTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCAcc                                                                                                       >  1:3069401/1‑101 (MQ=37)
  ccTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCt                                                                                                      >  2:1356017/1‑101 (MQ=37)
   tgcagcgcCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCtt                                                                                                     >  2:2187067/5‑101 (MQ=25)
   cTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCtt                                                                                                     <  1:3809830/101‑1 (MQ=37)
   cTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCtt                                                                                                     <  1:2513536/101‑1 (MQ=37)
    tGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTg                                                                                                    >  2:2185049/1‑101 (MQ=37)
     gcgcgcCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGc                                                                                                   <  2:4118282/101‑1 (MQ=37)
      cgcggCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGcc                                                                                                  <  2:2964949/101‑1 (MQ=25)
       gcgcCGTGGTCAGCGTTTAGGCAAGGGGGCGCTGCCCCC.C..CGGCTTCGCCTCACCCCCGGGGTATTTTTGGCAAGAT.AAACCCCATCCTTTCTCCTTTCCg                                                                                                 >  1:1575536/1‑101 (MQ=25)
       gcgcCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCg                                                                                                 >  1:3830234/1‑101 (MQ=37)
        cgcCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGc                                                                                                <  1:489274/101‑1 (MQ=37)
         gcCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCa                                                                                               <  2:2139799/101‑1 (MQ=37)
          ccGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCaa                                                                                              <  1:2874406/101‑1 (MQ=37)
          ccGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCaa                                                                                              >  1:1585085/1‑101 (MQ=37)
          ccGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCaa                                                                                              <  2:3000834/101‑1 (MQ=37)
           cGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCCCCCCCCGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCaaa                                                                                             >  1:777301/1‑101 (MQ=37)
            gTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGGTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAat                                                                                            >  2:2545248/1‑101 (MQ=37)
            gTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAat                                                                                            <  2:2241231/101‑1 (MQ=37)
            gTGGTCAGCGTTTAGGCAAGGGAGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAat                                                                                            <  2:4474152/101‑1 (MQ=37)
             tGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAata                                                                                           <  1:2986212/101‑1 (MQ=37)
             tGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAata                                                                                           <  2:4252110/101‑1 (MQ=37)
             tGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAata                                                                                           <  1:3162787/101‑1 (MQ=37)
             gggTCAGCGTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCGCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAata                                                                                           <  2:4362535/100‑1 (MQ=25)
              gggcAGGGTTTAGGGCAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAatat                                                                                          <  1:1531611/98‑1 (MQ=21)
              ggTCAGCGTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAatat                                                                                          <  1:4116336/101‑1 (MQ=37)
              ggTCAGCGTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAatat                                                                                          <  1:1129550/101‑1 (MQ=37)
               gTCAGCGTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATc                                                                                         <  2:568293/101‑1 (MQ=37)
               gTCAGCGTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATc                                                                                         <  1:2185199/101‑1 (MQ=37)
               gTCAGCGTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATc                                                                                         >  2:3435157/1‑101 (MQ=25)
                tCAGCGTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATcc                                                                                        >  1:4187963/1‑101 (MQ=37)
                 cAGCGTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATccc                                                                                       <  1:664412/101‑1 (MQ=37)
                 cAGCGTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATccc                                                                                       <  2:3555021/101‑1 (MQ=37)
                 cAGCGTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATccc                                                                                       >  1:3110031/1‑101 (MQ=37)
                  aGCGTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCg                                                                                      >  1:3606527/1‑101 (MQ=25)
                  aGCGTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCg                                                                                      <  1:3876810/101‑1 (MQ=37)
                   gCGTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCgg                                                                                     <  2:2272683/101‑1 (MQ=37)
                   gCGTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCgg                                                                                     <  1:186243/101‑1 (MQ=37)
                   gCGTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCgg                                                                                     <  1:442255/101‑1 (MQ=37)
                     gTTTAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCgggg                                                                                   <  1:447572/101‑1 (MQ=37)
                     ctgacggacggggggCTCTGC.CCC.C..CGGCTTCG.CTC.CCCCCGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCCaggggg                                                                                 >  2:2585515/11‑101 (MQ=2)
                     ctgacggacggggggCTCTGC.CCC.C..CGGCTTCG.CTC.CCCCCGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCCaggggg                                                                                 >  1:3167541/11‑101 (MQ=2)
                      tttAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCggggg                                                                                  >  1:3705785/1‑101 (MQ=37)
                       ttAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCgggggg                                                                                 <  2:4448374/101‑1 (MQ=37)
                        tAGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCCAATATCCCggggggc                                                                                >  2:567525/1‑100 (MQ=25)
                         aGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCgggggggg                                                                               >  1:541675/1‑99 (MQ=25)
                         aGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTg                                                                               <  1:457214/101‑1 (MQ=37)
                         aGGCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTg                                                                               <  1:2882259/101‑1 (MQ=37)
                          ggCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGAAAAAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGa                                                                              <  1:4653332/101‑1 (MQ=37)
                           gCAAGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGaa                                                                             <  1:1192725/101‑1 (MQ=37)
                             aaGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAAtt                                                                           <  1:762851/101‑1 (MQ=37)
                             aaGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAAtt                                                                           >  2:3190409/1‑101 (MQ=25)
                             aaGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAAtt                                                                           <  2:4271463/101‑1 (MQ=37)
                             aaGGGGGCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCGATCACCTTGCCGCAAATATCCCGGGGGGTGAAtt                                                                           >  1:4005449/1‑101 (MQ=25)
                               gggggCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTgg                                                                         >  1:3012954/1‑101 (MQ=37)
                                ggggCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGgc                                                                        <  2:3256091/101‑1 (MQ=37)
                                 gggCTCTG..CCCCC..CGGCTTCG.CTC.CCCCCGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGG.GAATTGgccaaa                                                                    >  1:3204317/1‑98 (MQ=2)
                                 gggCTCTG..CCCCC..CGGCTTCG.CTC.CCCCCGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGgccaaa                                                                    >  1:2983703/1‑98 (MQ=2)
                                 gggCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGgcc                                                                       >  2:4053354/1‑101 (MQ=37)
                                  ggCGCTG..CCC.CCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGgcc....g                                                                  <  2:3069157/101‑1 (MQ=37)
                                                                            tGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAGCGCCCCCTTTTCGgggcggg                             >  2:169142/1‑101 (MQ=14)
                                                                            tGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAGCGCCCCCTTTTCGgggcggg                             <  1:2480518/101‑1 (MQ=14)
                                                                             ggggCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAGCGCCCCCTTTTCGgggcgggc                            <  2:1302747/101‑1 (MQ=14)
                                                                              gggCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAGCGCCCCCTTTTCGGGGCgggcc                           >  2:4068038/1‑101 (MQ=14)
                                                                                 cAAGAT.AACCCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAGCGCCCCCTTTTCGGGGCgggccgca                        <  1:2988281/101‑1 (MQ=9)
                                                                                  aaGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAGCGCCCCCTTTTCGGGGCgggccgcag                       >  1:3519800/1‑101 (MQ=14)
                                                                                  aaGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAGCGCCCCCTTTTCGGGGCgggccgcag                       <  2:3513518/101‑1 (MQ=14)
                                                                                  aaGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGGGAATTGGCCGAAGGCCAAGAGGGGG..GCAGCGCCCCCTTTTCGGGGCgggccgcag                       >  1:3361931/1‑101 (MQ=9)
                                                                                   aGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAGCGCCCCCTTTTCGGGGCgggccgcagg                      >  1:4163937/1‑101 (MQ=14)
                                                                                    gAT.AAACCCCATCCTTTCCCCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAGCGCCCCCTTTTCGGGGCgggccgcaggg                     <  1:696988/101‑1 (MQ=9)
                                                                                    gAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCCGCGCCCCCTTTTCGGGGCgggccgccagg                     >  2:2901542/1‑97 (MQ=9)
                                                                                     aT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGGGAATTGGCCGAAGGCCAGGAGGGGG..GCAGCGCCCCCTTTTAGGGGCgggccgcagggc                    >  1:1387025/1‑101 (MQ=9)
                                                                                      t.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAGCGCCCCCTTTTCGGGGCgggccgcagggcc                   >  1:2445950/1‑101 (MQ=14)
                                                                                      t.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAGCGCCCCCTTTTCGGGGCgggccgcagggcc                   <  2:3334533/101‑1 (MQ=14)
                                                                                        aaaCCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAGCGCCCCCTTTTCGGGGCgggccgcagggccg                  >  2:1990618/1‑101 (MQ=14)
                                                                                        aaaCCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAGCGCCCCCTTTTCGGGGCgggccgcagggccg                  >  2:235268/1‑101 (MQ=14)
                                                                                         aaCCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAGCGCCCCCTTTTCGGGGCgggccgcagggccgc                 >  1:951809/1‑101 (MQ=14)
                                                                                         aaCCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAGCGCCCCCTTTTCGGGGCgggccgcagggccgc                 >  2:2594452/1‑101 (MQ=14)
                                                                                          aCCCCATCCTTTCTCCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAGCGCCCCCTTTTCGGGGCgggccgcagggccgca                <  1:2532924/101‑1 (MQ=9)
                                                                                           ccccATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCAc               <  2:211985/101‑1 (MQ=14)
                                                                                           ccccATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCAc               >  1:625181/1‑101 (MQ=14)
                                                                                           ccccATCCTTTCACCTTGCCGCAAATATCCCGGGGGGGGAATTGGCCGAAGGCCAAGAGGGGGTCA..GCGCGCCCTTTTCGGGGCGGGCCGCAGGGCCGCAc               >  2:2830202/1‑101 (MQ=9)
                                                                                           ccccATCCTTTCACCTTGCCGCAAATATCCCGGGGGGGGAATTGGCCGAAGGCCAAGAGGGGG..GCAGCGGACCCTTTTCGGGGCGGGCCGCAGGGCCGCAc               >  1:3428062/1‑101 (MQ=9)
                                                                                             ccATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCAccc             >  2:468793/1‑101 (MQ=14)
                                                                                               ctCCTTTCTCGTTGCGGTATATATCCTGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGT..GCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCCCCCTg           <  2:4553359/100‑1 (MQ=2)
                                                                                                 ccTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTc         >  1:244201/1‑101 (MQ=14)
                                                                                                  cTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCa        <  2:3417652/101‑1 (MQ=14)
                                                                                                  cTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCa        >  2:3139949/1‑101 (MQ=14)
                                                                                                   tttCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAg       <  2:3606259/101‑1 (MQ=14)
                                                                                                   tttCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAg       >  1:1922202/1‑101 (MQ=14)
                                                                                                    ttCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAgc      >  1:3545773/1‑101 (MQ=14)
                                                                                                     tccccTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAgcg     <  1:2659521/98‑1 (MQ=9)
                                                                                                     tCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAgcg     <  1:672721/101‑1 (MQ=14)
                                                                                                     tCACCTAGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAGCGCCCCCTTTTCGGGGCGGGGCGCAGGGCCGCACCCGGTCAgcg     >  2:1984083/1‑101 (MQ=9)
                                                                                                      cACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAgcgc    >  2:2595452/1‑101 (MQ=17)
                                                                                                        ccTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCAt  <  2:2129937/101‑1 (MQ=17)
                                                                                                        ccTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCAt  <  1:2833865/101‑1 (MQ=17)
                                                                                                        ccTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCAt  >  2:138254/1‑101 (MQ=17)
                                                                                                        ccTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGGCCAAGAGGGGG..GCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCAt  <  1:1950939/101‑1 (MQ=17)
                                                                                                        |                                                                                                      
ACCCTGCGCGCCGTGGTCAGCGTTTAGGCAAGGGGGCGCTG..CCT.C..CGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCC....GCCAAGATGGGG..G..GC.CCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCAT  >  NC_014034/1303787‑1303978

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG