Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,329,189 C→G 18.8% intergenic (‑127/+114) RCAP_rcc01255 ← / ← RCAP_rcc01256 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,329,1890C→G18.8% 21.3 ‑3.4 ‑0.0108intergenic (‑127/+114)RCAP_rcc01255/RCAP_rcc01256hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (2/20):  ref base (27/58):  total (29/79)
Fisher's exact test strand distribution p-value = 3.42e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.15e-01

Only 100 of 175 total aligned reads displayed.

GATGGGCGCGCAGCAGGTAGCCTTCCGCGCATCGAATCCCGCCTGCTACCTCTCGACGATGGGCAACAGGGGGGCTTTGTGGCTCGCCCCGAGC.GGGGGCGAGCCGTGATTACACGTGCGGGCGGTGATGGGAGCCCAGTGCGTGGGCGATGGAGAGCCGTGTGGAGCATGACCCGCCGAAGAGCGGGCGGCTGCAGCC  >  NC_014034/1329090‑1329288
                                                                                                    |                                                                                                   
gATGGGCGCGCAGCAGGTAGCCTTCCGCGCATCGAATCCCGCCTGCTACCTCTCGACGATGGGCAACAGGGGGGCTTTGTGGCTCGCCCCGAGC.GGGGGCg                                                                                                    <  1:87261/101‑1 (MQ=255)
 aTGGGCGCGCAGCAGGTAGCCTTCCGCGCATCGAATCCCGCCTGCTACCTCTCGACGATGGGCAACAGGGGGGCTTTGTGGCTCGCCCCGAGC.GGGGGCGa                                                                                                   <  1:1232716/101‑1 (MQ=255)
   gggCGCGCAGCAGGTAGCCTTCCGCGCATCGAATCCCGCCTGCTACCTCTCGACGATGGGCAACAGGGGGGCTTTGTGGCTCGCCCCGAGC.GGGGGCGAGc                                                                                                 <  1:2420925/101‑1 (MQ=255)
   gggCGCGCAGCAGGTAGCCTTCCGCGCATCGAATCCCGCCTGCTACCTCTCGACGATGGGCAACAGGGGGGCTTTGTGGCTCCCCCCCCGG.GGGGGGGAGc                                                                                                 >  2:4427805/1‑101 (MQ=255)
      ggggcggcagGGCGCCTTCCGCGCATCGAACCCCCCCTGCTGCCCCTCGGCGAGGGGCAGCGGGGGGCCTTTGGGGCTCGCCCCGAGC.GGGGGCGAGCCGt                                                                                              <  2:2482706/95‑1 (MQ=255)
      cgcgcAGCAGGTAGCCTTCCGCGCATCGAATCCCGCCTGCTACCTCTCGACGATGGGCAACAGGGGGGCTTTGTGGCTCGCCCCGAGC.GGGGGCGAGCCGt                                                                                              <  2:1105467/101‑1 (MQ=255)
       gcgcAGCAGGTAGCCTTCCGCGCATCGAATCCCGCCTGCTACCTCTCGACGATGGGCAACAGGGGGGCTTTGTGGCTCGCCCCGAGC.GGGGGCGAGCCGTg                                                                                             <  1:2253990/101‑1 (MQ=255)
       gcgcAGCAGGTAGCCTTCCGCGCATCGAATCCCGCCTGCTACCTCTCGACGATGGGCAACAGGGGGGCTTTGTGGCTCGCCCCGAGC.GGGGGCGAGCCGTg                                                                                             <  2:60374/101‑1 (MQ=255)
        cgcAGCAGGTAGCCTTCCGCGCATCGAATCCCGCCTGCTACCTCTCGACGATGGGCAACAGGGGGGCTTTGTGGCTCCCCCCCCGC.GGGGGCGGGCCgggg                                                                                            >  1:1885446/1‑98 (MQ=255)
          cagcagGTAGCCTTCCGCGCATCGAATCCCGCCTGCTACCTCTCGACGATGGGCAACAGGGGGGCTTTGTGGCTCCCCCCGACC.GGGGGCGGGCCGGGAtt                                                                                          >  1:4578479/1‑101 (MQ=255)
           agcagGTAGCCTTCCGCGCATCGAATCCCGCCTGCTACCTCTCGACGATGGGCAACAGGGGGGCTTTGTGGCTCGCCCCGAGC.GGGGGCGAGCCGTGATTa                                                                                         <  2:1980085/101‑1 (MQ=255)
             cagGTAGCCTTCCGCGCATCGAATCCCGCCTGCTACCTCTCGACGATGGGCAACAGGGGGGCTTTGTGGCTCGCCCCGAGC.GGGGGCGAGCCGTGATTaca                                                                                       <  1:3603127/101‑1 (MQ=255)
             cagGTAGCCTTCCGCGCATCGAATCCCGCCTGCTACCTCTCGACGATGGGCAACAGGGGGGCTTTGTGGCTCGCCCCGAGC.GGGGGCGAGCCGTGATTaca                                                                                       <  1:79457/101‑1 (MQ=255)
              agGTACCCTGCCGCGCATCGAATCCCGCCTGCTCCCTCTCGGCGATGGGCAACAGGGGGGCTTTGGGGCTCGCCCCGAGC.GGGGGCGAGCCGTGATTacac                                                                                      <  2:2907172/101‑1 (MQ=255)
                gTAGCCTTCCGCGCATCGAATCCCGCCTGCTACCCCTCGACGATGGGCAACAGGGGGGCTTTGTGGCTCGCCCCGGGC.GGGGGCGAGCCGTGATTACACGt                                                                                    <  1:2548324/101‑1 (MQ=255)
                gTAGCCTTCCGCCCATCGAATCCCGCCTGCTACCTCTCGGCGATGGGCAACAGGGGGGTTTTGTGGCTCGCCCCGAGC.GGGGGCGAGCCGTGATTACACGt                                                                                    <  1:477314/101‑1 (MQ=255)
                    ccTTCCGCGCATCGAATCCCGCCTGCTACCTCTCGACGATGGGCAACAGGGGGGCTTTGTGGCTCGCCCCGAGC.GGGGGCGAGCCGTGATTACACGTgcgg                                                                                <  1:1455608/101‑1 (MQ=255)
                    ccTTCCGCGCATCGAACCCCGCCTGCTACCTCTCGACGATGGGCAGCAGGGGGGCTTTGTGGCTCGCCCCGAGC.GGGGGCGAGCCGTGATTACACGTgcgg                                                                                <  2:2737910/101‑1 (MQ=255)
                     cTTCCGCGCATCGAATCCCGCCTGCTACCTCTCGACGATGGGCAACAGGGGGGCTTTGTGGCTCCCCCCCGGC.GGGGGGGGGCCGGGGGTTCACCTgcggg                                                                               >  2:4302613/1‑101 (MQ=255)
                     cTTCCGCGCATCGAACCCCGCCTGCTCCCTCTCGACGATGGGCAACAGGGGGGCTTTGTGGCTCGCCCCGAGC.GGGGGCGAGCCGTGATTACACGTgcggg                                                                               <  1:683176/101‑1 (MQ=255)
                        ccGCGCATCGAATCCCGCCTGCTACCTCTCGACGATGGGCAACAGGGGGGCTTTGTGGCTCCCCCCCACC.GGGGGCGCGCCCGGATTTAACGTgcgggggg                                                                            >  2:2263814/1‑101 (MQ=255)
                             cagcGAATCCCGCTTGATACCTCTCAGCTCGGGAAAACAGGGGGCGTTGGTGACTCCCCCCGAGG.GGGGGCGAGCCGTGATTACACGTGCGGGCGGTGATg                                                                       <  2:4608795/98‑1 (MQ=255)
                             cATCGAATCCCGCCTGCTACCTCTCGACGATGGGCAACAGGGGGGCTTTGTGGCTCCCCCCGAGC.GGGGGGGGGCCGGGGTTACACGTgcgggggggggtg                                                                       >  1:4251751/1‑96 (MQ=255)
                              agcGAACCCCGCCTGCTACCTCTCGACGATGGGCAACAGGGGGGCTTTGTGGCTCGCCCCGAGC.GGGGGCGAGCCGTGATTACACGTGCGGGCGGTGATgg                                                                      <  2:3300470/99‑1 (MQ=255)
                                 gAATCCCGCCTGCTACCTCTCGACGATGGGCAACAGGGGGGCTTTGTGGCTCGCCCCCGGC.GGGGGGGGGCCGTGGTTACACGTGCGGGCGGGGAGGGGAg                                                                   >  1:4166730/1‑101 (MQ=255)
                                  acccccGCCTGCTCCCTCTCGACGATGGGCAACAGGGGGGCTTTGTGGCTCGCCCCGGGG.GGGGGCGAGCCGTGATTACACGTGCGGGCGGTGATGGGAGc                                                                  <  1:1054806/98‑1 (MQ=255)
                                    tCCCGCCTGCTACCTCTCGACGATGGGCAACAGGGGGGCTTTGTGGCTCGCCCCCAGC.GGGGGGGGGGCGTGATTACACGTGCGGGCGGGGAGGGGGGccc                                                                >  2:1732058/1‑101 (MQ=255)
                                    tCCCGCCTGCTACCTCTCGACGATGGGCAACAGGGGGGCTTTGTGGCTCCCCCCCAGC.GGGGGGGGGCCGGGATTAAACGTGCGGGCGGTGATGGGGGccc                                                                >  2:3830146/1‑101 (MQ=255)
                                     cccGCCTGCTCCCTCTCGACGATGGGCAACGGGGGGGCTTTGTGGCTCGCCCCGAGG.GGGGGCGAGCCGTGATTACACGTGCGGGCGGTGATGGGAGCCCa                                                               <  1:3120578/101‑1 (MQ=255)
                                     cccGCCTGCTACCTCTCGACGATGGGCAACAGGGGGGCTTTGTGGCTCGCCCCGAGG.GGGGGCGAGCCGTGATTACACGTGCGGGCGGTGATGGGAGCCCa                                                               <  2:468348/101‑1 (MQ=255)
                                     cccGCCTGCTACCTCTCGACGATGGGCAACAGGGGGGCTTTGTGGCTCCCCCCCCGC.GGGGGGGGGGCGGGATTACACCTGCGGGGGGGGGTGGGCGcccc                                                               >  2:137024/1‑100 (MQ=255)
                                       cGCCTGCTCCCTCGCGAGGGTGGGCAACGGGGGGGCTTTGGGGCGCGCCCCGGGC.GGGGGGGAGCCGTGATTACACGTGCGGGCGGTGATGGGAGCCCAGt                                                             <  1:935616/101‑1 (MQ=255)
                                       cGCCTGCTACCTCTCGACGATGGGCAACAGGGGGGCTTTGTGGCTCGCCCCCAGC.GGGGGGGAGCCGGGATTACACATGCGGGCGGTGGTGGGCGCCCAgc                                                             >  2:2590625/1‑100 (MQ=255)
                                       cGCCTGCTACCTCTCGACGATGGGCAACAGGGGGGCTTTGTGGCTCCCCCCGCGC.GGGGGGGAGCCGGGATTAACCGTGGGGGGGGGGGGGGGGGccccgg                                                             >  1:3295033/1‑98 (MQ=255)
                                        cccTGCTCCCTCTCGACGAGGGGCAACGGGGGGGCTTTGGGGCTCGCCCCGAGC.GGGGGCGAGCCGTGATTACACGTGCGGGCGGTGATGGGAGCCCAGTg                                                            <  2:3958751/100‑1 (MQ=255)
                                         ccTGCTCCCTCTGGACGATGGCCAACAGGGGGGCTTTGTGCCCCGCCCCGAGG.GGGGGCGAGCCGTGATTACACGTGCGGGCGGTGATGGGAGCCCAGTGc                                                           <  2:469113/101‑1 (MQ=255)
                                           tGCTACCTCTCGACGATGGGCAACAGGGGGGCTTTGTGGCTCGCCCCGAGC.GGGGGCGAGCCGGGATTACACGTGCGGGCGGGGATGGGAGGCCCGTGCgg                                                         >  2:2517975/1‑100 (MQ=255)
                                           tGCTACCTCTCGACGATGGGCAACAGGGGGGCTTTGTGGCTCCCCCCCCGC.GGGGGGGGGCCGGGGTTACAAGGGCGGGGGGGGGTGGGGGccccgggggg                                                         >  1:4073023/1‑94 (MQ=255)
                                            gCTACCTCTCGACGATGGGCAACAGGGGGGCTTTGTGGCTCGCCCCCCGC.GGGGGGGAGCCGCGGGTACACGTGCGGGCGGGGGGGGGGGCCCCGTGGGTg                                                        >  1:1009091/1‑101 (MQ=255)
                                            gCTACCTCTCGACGATGGGCAACAGGGGGGCTTTGTGGCTCCCCCCGCGG.GGGGGGGAGCTGTGATTATACGTGCGGGCGGGGTGGGGTGCGCAGGGCggg                                                        >  2:742878/1‑99 (MQ=255)
                                            gCTACCTCTCGACGATGGGCAACAGGGGGGCTTTGTGGCTCCCCCCCAGC.GGGGGGCGGCCGCGAGTACACCTGCCGGCGGGGGTGGGGGGCCacgccggg                                                        >  1:2655412/1‑94 (MQ=255)
                                             ctcccTCTCGACGATGGGCAACGGGGGGGCTTTGTGGCTCGCCCCGAGC.GGGGGCGAGCCGTGATTACACGTGCGGGCGGTGATGGGAGCCCAGTGCGTgg                                                       <  1:2711689/98‑1 (MQ=255)
                                              tACCTCTCGACGATGGGCAACAGGGGGGCTTTGTGGCTCGCCCCCGGC.GGGGGGGAGCCGGGGTTACACGCGGGGGCGGGGATGGGGGCCCCGGGCGGggg                                                      >  1:1715154/1‑101 (MQ=255)
                                              tACCTCTCGACGATGGGCAACAGGGGGGCTTTGTGGCTCCCCCCCAGC.GGGGGCGAGCCGTGGTTACACGTGCGGGCGGGGATGGGAGGCCAGTGCGTggg                                                      >  1:123834/1‑101 (MQ=255)
                                               aCCTCTCGACGATGGGCAACAGGGGGGCTTTGTGGCTCCCCCCGCGC.GGGGGCGAGCCGTGATTACACGTGCGGGCGGGGATGGGAGCCCAGTGCGAgggg                                                     >  2:198488/1‑100 (MQ=255)
                                                  tctcAACGAGGGGCAATGGGGGGGTTTTGGGGCGCCCCCCGAGC.GGGGGCGAGCCGTGATTACACGTGCGGGCGGTGATGGGAGCCCAGTGCGTGGGCGAt                                                  <  1:753119/101‑1 (MQ=255)
                                                   ctggacgaTGGGCAAGGGGGGGGCTTTGGGGCTCGCCCCGAGG.GGGGGCGAGCCGTGATTACACGTGCGGGCGGTGATGGGAGCCCAGTGCGTGGGCGATg                                                 <  1:3268581/98‑1 (MQ=255)
                                                   ctcGACGATGGGCAACAGGGGGGCTTTGTGGCTCGCCCCGCGC.GGGGGCGGGGCGTGATTACACGTGCGGGCGGGGGTGGGGGCCCCGTGCGTGGGGGATg                                                 >  2:486986/1‑101 (MQ=255)
                                                   cccgaggaTGGGCAACGGGGGGGTTTTGTGGCTCGCCCGGGGG.GGGGGCGAGCCGTGATTACACGTGCGGGCGGTGATGGGAGCCCAGTGCGTGGGCGATg                                                 <  1:1375799/99‑1 (MQ=255)
                                                    tcGACGATGGGCAACAGGGGGGCTTTGTGGCTCGCCCCCAGC.GGGGGGGGGCCGGGATTAAACGTGCGGGCGGGGATGGGAGGCCCGGGCGTGGGGGATgg                                                >  1:3759564/1‑101 (MQ=255)
                                                     cgacgaTGGGCAACGGGGGGGCTTGGTGGCTCGCCCCGGGC.GGGGGGGAGCCGTGATTACACGTGCGGGCGGTGATGGGAGCCCAGTGCGTGGGCGATGga                                               <  1:3017949/101‑1 (MQ=255)
                                                     cgacgaTGGGCAACAGGGGGGCTTTGTGGCTCGCCCCCAGC.GGGGGCGAGCCGTGATTACACGTGCGGGCGGGGGTGGGAGCCCAGGGCGTGGGGGGTggg                                               >  1:159989/1‑100 (MQ=255)
                                                     cgacgaTGGGAAACGGGGGGGCTTTGGGGCTCGCCCCGAGG.GGGGGCGAGCCGTGATTACACGTGCGGGCGGTGATGGGAGCCCAGTGCGTGGGCGATGga                                               <  2:144061/101‑1 (MQ=255)
                                                      gacgaTGGGCAACAGGGGGGCTTTGTGGCTCCCCCCCAGC.GGGGGGGGGGCGGGATTACACGTGCGGGGGGGGATGGGAGCCCAGTGCGTGGGGGAGgggg                                              >  1:3068023/1‑99 (MQ=255)
                                                        cgaTGGGCAACAGGGGGGCTTTGTGGCTCGCCCCGCGC.GGGGGGGGGCGGTGATTACACATGCGGGCGGGGAGGGGAGCCCCGTGCGGGGGCGGTGGcgag                                            >  1:421896/1‑101 (MQ=255)
                                                        cgaTGGGCAACAGGGGGGCTTTGTGGCTCCCCCCCCGC.GGGGGGGGGCCGGGGTTAAAACGGCGGGCGGGGGTGGGGGGCCAGCGCGGgggggggggggag                                            >  2:1580268/1‑91 (MQ=255)
                                                         gaTGGGCAACAGGGGGGCTTTGTGGCTCGCCCCCAGC.GGGGGCGAGCCGTGATTACACGTGCGGGCGGGGGTGGGAGGCCAGTGCGGGGGCGGGGGAGAGc                                           >  2:2096651/1‑101 (MQ=255)
                                                          aTGGGCAACAGGGGGGCTTTGTGGCTCGCCCCCAGC.GGGGGGGAGCCGGGATTACACGTGCGGGCGGGGATGGGGGCCCCGGGCGTGGGCGATGGGGAGcc                                          >  1:3725448/1‑101 (MQ=255)
                                                           tGGGCAACAGGGGGGCTTTGTGGCTCGCCCCGAGC.GGGGGGGGGGCGGGATTACACGTGCGGGCGGGGGTGGGGGCCCAGTGCGGGGGCGATGgaggggcg                                         >  2:1295615/1‑96 (MQ=255)
                                                           tGGGCAACAGGGGGGCTTTGTGGCTCGCCCCGAGC.GGGGGGGAGCCGTGGTTACACGTGCGGGCGGGGATGGGGGCCCAGTGCGTGGGGGGTGGAGAGGCg                                         >  1:2184646/1‑101 (MQ=255)
                                                            gggCACCGGGGGGGCTTGGTGGCCCGCCCCGGGG.GGGGGGGAGCCGTGATTACACGTGCGGGCGGTGATGGGAGCCCAGTGCGTGGGCGATGGAGAGCCgt                                        <  1:1398534/101‑1 (MQ=255)
                                                            gggCAAAGGGGGGGTTTTGTGGCCCGCCCGGAGG.GGGGGGGAGCCGTGATTACACGTGCGGGCGGTGATGGGAGCCCAGTGCGTGGGCGATGGAGAGCCgt                                        <  1:1921241/101‑1 (MQ=255)
                                                             ggCAACAGGGGGGCTTTGTGGCTCCCCCCCAGC.GGGGGCGGGCCCGGGTTACACCTGCGGGCGGGGGTGGGGGGCCAGTGCGTGGGGGGTGGGGGGCCggg                                       >  1:4406020/1‑99 (MQ=255)
                                                               cAACAGGGGGGCTTTGGGGCTCGCCCCGAGC.GGGGGCGAGCCGTGATTACACGTGCGGGCGGGGATGGGAGCCCAGTGCGGGGGGGATGGAGAGGCgtggg                                     >  1:4076257/1‑99 (MQ=255)
                                                                aaCAGGGGGGCTTTGTGGCTCGCCCCGAGC.GGGGGGGAGCCGGGATTACACGTGCGGGCGGGGGTGGGGGCCCAGTGCGTGGGCGGTGGGGAGCCGGGTgg                                    >  2:3222890/1‑101 (MQ=255)
                                                                aaCAGGGGGGCTTTGTGGCTCGCCCCGAGC.GGGGGGGAGCCGGGATTACACGTGCGGGCGGGGATGGGAGCCCAGTGCGTGGGCGGTGGAGAGCCGTGGgg                                    >  2:2945121/1‑101 (MQ=255)
                                                                  cAGGGGGGCTTTGTGGCTCGCCCCGAGC.GGGGGCGAGCCGGGATTACACGTGCGGGCGGGGATGGGGGCCCCGGGCGGGGGCGGTGGAGGGCCCGGTgggg                                  >  1:1272908/1‑99 (MQ=255)
                                                                  cAGGGGGGCTTTGTGGCTCGCCCCCAGC.GGGGGCGAGCCGTGATTACACGTGGGGGCGGGGATGGGAGCCCAGGGGGTGGGGGATGGAGGGCCgggggggg                                  >  1:4654638/1‑94 (MQ=255)
                                                                     gggggCTTTGTGGCTCGCCCCGAGC.GGGGGCGAGCCGGGATTACACGTGCGGGGGGGGATGGGGGGCCAGTGCGTGGGCGATGGAGAGCCGGGGGGAGCAt                               >  2:4394003/1‑101 (MQ=255)
                                                                        ggCTTTGTGGCTCGCCCCGAGC.GGGGGCGAGCCGTGATTACACGTGCGGGCGGGGATGGGAGCCCCGTGCGTGGGCGATGGAGAGCCGTGTGGAGGATGAc                            >  2:3633655/1‑101 (MQ=255)
                                                                         gtgtgggggCTCGCCGGGAGGGGGGGGGGCGCCTTGTTTCAACGTGC.GGCGGTGATGGGAGCCCAGTGCGTGGGCGATGGAGAGCCGTGTGGAGCATGAcc                           <  1:4465355/94‑1 (MQ=255)
                                                                         gCTTTGTGGCTCGCCCCGGGC.GGGGGGGAGCCGTGATTACACGTGCGGGCGGTGATGGGAGCCCAGTGCGTGGGCGATGGAGAGCCGTGTGGAGCATGAcc                           <  2:3494444/101‑1 (MQ=255)
                                                                         gCTTTGTGGCTCGCCCCGAGC.GGGGGCGAGCCGTGATTACACGTGCGGGCGGTGATGGGAGCCCAGTGCGTGGGCGGTGGAGAGCCGGGTGGGGGATGAcc                           >  2:2845773/1‑101 (MQ=255)
                                                                           tttgggccccGCCCGGGGC.GGGGGCGAGCCGTGATTACACGTGCGGGCGGTGATGGGAGCCCAGTGCGTGGGCGATGGAGAGCCGTGTGGAGCATGACccg                         <  1:477152/92‑1 (MQ=255)
                                                                            ttGTGGCTCGCCCCGAGC.GGGGGCGAGCCGTGATTACACGTGCGGGCGGTGATGGGAGCCCAGTGCGTGGGCGATGGAGAGCCGTGTGGGGCATGACccgc                        >  2:742279/1‑101 (MQ=255)
                                                                             tgtgGCTCGCCCCGAGC.GGGGGCGAGCCGTGATTACACGTGCGGGCGGGGATGGGAGGCCCGTGCGTGGGCGGTGGGGAGCCGGGTGGGGCATGACccgcc                       >  2:3467625/1‑101 (MQ=255)
                                                                              gtgGCCCGCCCCGGGG.GGGGGCGAGCCGTGATTACACGTGCGGGCGGTGATGGGAGCCCAGTGCGTGGGCGATGGAGAGCCGTGTGGAGCATGACccgccg                      <  2:396257/101‑1 (MQ=255)
                                                                               tgGCTCGCCCCGAGC.GGGGGCGAGCCGTGATTACACGTGCGGGCGGTGATGGGAGCCCAGTGCGTGGGCGATGGAGAGCCGGGTGGAGCATGACccgccca                     >  2:4435504/1‑99 (MQ=255)
                                                                                 gCTCGCCCCGAGC.GGGGGCGAGCCGTGATTACACGTGCGGGCGGTGATGGGAGCCCAGTGCGTGGGCGATGGAGAGCCGTGTGGAGCATGACCCGCCGAag                   >  2:3170281/1‑101 (MQ=255)
                                                                                  cTCGCCCCGAGC.GGGGGCGAGCCGTGATTACACGTGCGGGCGGTGATGGGAGCCCAGTGCGTGGGCGATGGAGAGCCGTGTGGAGCATGACCCGCCGAaga                  >  2:3060216/1‑101 (MQ=255)
                                                                                   tCGCCCCGAGC.GGGGGCGAGCCGTGATTACACGTGCGGGCGGTGATGGGAGCCCAGTGCGTGGGCGATGGAGAGCCGTGTGGAGCATGACCCGCCGAagag                 >  1:3603576/1‑101 (MQ=255)
                                                                                     gCCCCGAGC.GGGGGCGAGCCGTGATTACACGTGCGGGCGGTGATGGGAGCCCAGTGCGTGGGCGATGGAGAGCCGTGTGGAGCATGACCCGCCGAAGAgcg               >  2:810718/1‑101 (MQ=255)
                                                                                     gCCCCGAGC.GGGGGCGAGCCGTGATTACACGTGCGGGCGGTGATGGGAGCCCAGTGCGTGGGCGATGGAGAGCCGTGTGGAGCATGACCCGCCGAAGAgcg               >  2:2589452/1‑101 (MQ=255)
                                                                                     gCCCCGAGC.GGGGGCGAGCCGTGATTACACGTGCGGGCGGTGATGGGAGCCCAGTGCGTGGGCGATGGAGAGCCGTGTGGAGCATGACCCGCCGAAGAgcg               >  1:2493075/1‑101 (MQ=255)
                                                                                     gCCCCGAGC.GGGGGCGAGCCGTGATTACACGTGCGGGCGGTGATGGGAGCCCAGTGCGTGGGCGATGGAGAGCCGGGTGGAGCATGACCCGCCGAAGAgcg               >  2:4580515/1‑101 (MQ=255)
                                                                                     gCCCCGAGC.GGGGGCGAGCCGTGATTACACGTGCGGGCGGCGATGGGAGCCCAGTGCGTGGGCGATGGAGAGCCGTGTGGAGCATGACCCGCCGAAGAgcg               >  1:1007710/1‑101 (MQ=255)
                                                                                     cccccggggggggggCGAGCCGTGATTACACGTGCGGGCGGTGATGGGAGCCCAGTGCGTGGGCGATGGAGAGCCGTGTGGAGCATGACCCGCCGAAGAgc                <  2:3385329/91‑1 (MQ=255)
                                                                                       cccggggggggggCGAGCCGTGATTACACGTGCGGGCGGTGATGGGAGCCCAGTGCGTGGGCGATGGAGAGCCGTGTGGAGCATGACCCGCCGAAGAgcgg              <  2:879140/93‑1 (MQ=255)
                                                                                       cccGGGG.GGGGGGGAGCCGTGATTACACGTGCGGGCGGTGATGGGAGCCCAGTGCGTGGGCGATGGAGAGCCGTGTGGAGCATGACCCGCCGAAGAgcggg             <  2:3232553/101‑1 (MQ=255)
                                                                                        ccggggggggggGGAGCCGTGATTACACGTGCGGGCGGTGATGGGAGCCCAGTGCGTGGGCGATGGAGAGCCGTGTGGAGCATGACCCGCCGAAGAgcggg             <  1:2096791/94‑1 (MQ=255)
                                                                                        ccGAGC.GGGGGCGAGCCGTGATTACACGTGCGGGCGGTGATGGGAGCCCAGTGCGTGGGCGATGGAGAGCCGTGTGGAGCATGACCCGCCGAAGAgcgggc            >  1:612338/1‑101 (MQ=255)
                                                                                          gAGC.GGGGGCGAGCCGTGATTACACGTGCGGGCGGTGATGGGAGCCCAGTGCGTGGGCGATGGAGAGCCGTGTGGAGCATGACCCGCCGAAGAgcgggcgg          >  2:1877689/1‑101 (MQ=255)
                                                                                          gAGC.GGGGGCGAGCCGTGATTACACGTGCGGGCGGTGATGGGAGCCCAGTGCGTGGGCGATGGAGAGCCGTGTGGAGCATGACCCGCCGAAGAgcgggcgg          >  1:4447460/1‑101 (MQ=255)
                                                                                           aGC.GGGGGCGAGCCGTGATTACACGTGCGGGCGGTGATGGGAGCCCAGTGCGTGGGCGATGGAGAGCCGTGTGGAGCATGACCCGCCGAAGAGCGggcggc         >  2:2401738/1‑101 (MQ=255)
                                                                                            ggggggggGGAGCCGTGATTACACGTGCGGGCGGTGATGGGAGCCCAGTGCGTGGGCGATGGAGAGCCGTGTGGAGCATGACCCGCCGAAGAGCGggcggc         <  1:1104777/98‑1 (MQ=255)
                                                                                             ggtcgggGGAGCCGTGATTACACGTGCGGGCGGTGATGGGAGCCCAGTGCGTGGGCGATGGAGAGCCGTGTGGAGCATGACCCGCCGAAGAGCGGGCGGCt        <  1:1848552/97‑1 (MQ=255)
                                                                                             gggggggGGAGCCGTGATTACACGTGCGGGCGGTGATGGGAGCCCAGTGCGTGGGCGTTGGAGAGCCGTGTGGAGCATGACCCGCCGAAGAGCGGGCGGCt        <  2:2397372/99‑1 (MQ=255)
                                                                                               gggggCGAGCCGTGATTACACGTGCGGGCGGTGATGGGAGCCCAGTGCGTGGGCGATGGAGAGCCGTGTGGAGCATGACCCGCCGAAGAGCGGGCGGCTga      >  1:4257046/1‑100 (MQ=255)
                                                                                                ggggGGAGCCGTGATTACACGTGCGGGCGGTGATGGGAGCCCAGTGCGTGGGCGATGGAGAGCCGTGTGGAGCATGACCCGCCGAAGAGCGGGCGGCTGCa     <  1:4328042/101‑1 (MQ=255)
                                                                                                  ggCGAGCCGTGATTACACGTGCGGGCGGTGATGGGAGCCCAGTGCGTGGGCGATGGAGAGCCGTGTGGAGCATGACCCGCCGAAGAGCGGGCGGCtagatc   >  2:1848426/1‑96 (MQ=255)
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GATGGGCGCGCAGCAGGTAGCCTTCCGCGCATCGAATCCCGCCTGCTACCTCTCGACGATGGGCAACAGGGGGGCTTTGTGGCTCGCCCCGAGC.GGGGGCGAGCCGTGATTACACGTGCGGGCGGTGATGGGAGCCCAGTGCGTGGGCGATGGAGAGCCGTGTGGAGCATGACCCGCCGAAGAGCGGGCGGCTGCAGCC  >  NC_014034/1329090‑1329288

Base quality scores:  ATCG  < 3 ≤  ATCG  < 8 ≤  ATCG  < 21 ≤  ATCG  < 32 ≤  ATCG  < 40 ≤  ATCG