Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,400 A→T 8.1% intergenic (+212/‑719) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,4000A→T 8.1% 47.9 ‑0.2 ‑1.3248intergenic (+212/‑719)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (12/8):  ref base (125/98):  total (137/111)
Fisher's exact test strand distribution p-value = 8.17e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.55e-02

Only 100 of 268 total aligned reads displayed.

GCGACAAGCACCTTCGCACCACCGGAGCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAAC  >  NC_014034/1395300‑1395497
                                                                                                    |                                                                                                  
gCGACAAGCACCTTCGCACCACCGGAGCAATTTTCACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCa                                                                                                    <  1:3976830/101‑1 (MQ=255)
 cGACAAGCACCTTCGCACCACCGGAGCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCAc                                                                                                   <  1:4330052/101‑1 (MQ=255)
   aCAAGCACCTTCGCACCACCGGAGCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCAccc                                                                                                 >  2:4210144/1‑101 (MQ=255)
   aCAAGCACCTTCGCACCACCGGAGCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCAccc                                                                                                 <  2:37597/101‑1 (MQ=255)
    cAAGCACCTTCGCACCACCGGAGCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCg                                                                                                >  2:1173707/1‑101 (MQ=255)
     aaGCACCTTCGCACCACCGGAGCAATTTACACCATCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGc                                                                                               <  1:4000112/101‑1 (MQ=255)
       gCACCTTCGCACCACCGGAGCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTg                                                                                             >  1:1963776/1‑101 (MQ=255)
       gCACCTTCGCACCACCGGAGCAATTTACACCGTCTGGTCAGCGGGTTAAGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTg                                                                                             <  1:1769628/101‑1 (MQ=255)
         cccTTCGCACCACCGGAGCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGc                                                                                           <  2:2755601/100‑1 (MQ=255)
           cTTCGCACCACCGGAGCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGccc                                                                                         <  1:1162764/101‑1 (MQ=255)
           cTTCGCACCACCGGAGCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGccc                                                                                         >  1:3244454/1‑101 (MQ=255)
            ttCGCACCACCGGAGCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCt                                                                                        >  1:2979026/1‑101 (MQ=255)
            ttCGCACCACCGGAGCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCt                                                                                        >  1:711289/1‑101 (MQ=255)
            ttCGCACCACCGGAGCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCt                                                                                        >  2:1837436/1‑101 (MQ=255)
              cGCACCACCGGAGCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGc                                                                                      >  1:1849249/1‑101 (MQ=255)
              cGCACCACCGGAGCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGc                                                                                      >  2:2965934/1‑101 (MQ=255)
               gCACCACCGGAGCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGcc                                                                                     <  1:2000769/101‑1 (MQ=255)
               gCACCACCGGAGCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGAACCCGCTGGCCCTGcc                                                                                     >  2:3981013/1‑101 (MQ=255)
                caccacCGGAGCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCcg                                                                                    >  2:2342682/1‑101 (MQ=255)
                 accaccGGAGCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCcgg                                                                                   <  2:2851814/101‑1 (MQ=255)
                 accaccGGAGCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCcgg                                                                                   >  2:1249333/1‑101 (MQ=255)
                   caccGGAGCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCcggtg                                                                                 <  1:540035/101‑1 (MQ=255)
                      cGGAGCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCcggtgatc                                                                              >  2:1874739/1‑101 (MQ=255)
                      cGGAGCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGCGGGCGTGGATCGAAACTCCACCCGCTGGCCCCGCcggtgaac                                                                              >  1:883350/1‑99 (MQ=255)
                       ggAGCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCcggtgatcg                                                                             >  2:2061357/1‑101 (MQ=255)
                       ggAGCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCcggtgatcg                                                                             >  2:2110342/1‑101 (MQ=255)
                         aGCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCcggtgatcggt                                                                           >  1:2986407/1‑101 (MQ=255)
                            aaTTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCcggtgatcggtg.at                                                                       <  2:2024427/101‑1 (MQ=255)
                             aTTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCggtgatcggtg.atc                                                                      >  1:3878072/1‑101 (MQ=255)
                              tttACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCa                                                                     >  1:3900809/1‑101 (MQ=255)
                              tttACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGGGATCGGTG.ATCa                                                                     >  2:4031935/1‑101 (MQ=255)
                               ttACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGGCCTGCCGGTGATCGGTG.ATCAg                                                                    >  1:4574207/1‑101 (MQ=255)
                               ttACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAg                                                                    >  1:4300910/1‑101 (MQ=255)
                               ttACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAg                                                                    <  1:2484104/101‑1 (MQ=255)
                               ttACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAg                                                                    >  2:1814577/1‑101 (MQ=255)
                                 acacCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCg                                                                  >  2:1755697/1‑101 (MQ=255)
                                 acacCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGAACCCGCTGGCCCTGCCGGGGCTCGGGG.GTCAggg                                                                  >  1:3418179/1‑99 (MQ=37)
                                   cccGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGt                                                                <  1:1106706/100‑1 (MQ=255)
                                   acCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCggc                                                                >  2:3178039/1‑100 (MQ=255)
                                     cGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCg                                                              <  1:3351839/101‑1 (MQ=255)
                                     cGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCg                                                              <  1:823746/101‑1 (MQ=255)
                                        cTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTc                                                           <  1:3664559/101‑1 (MQ=255)
                                          ggTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTccc                                                         >  2:3968839/1‑101 (MQ=255)
                                          ggTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGGGATCGGTG.ATCAGCGGTCGGTccc                                                         >  2:3368734/1‑101 (MQ=255)
                                           gTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGGG.ATCAGCGGTCGCTcccc                                                        >  2:352498/1‑101 (MQ=255)
                                              aGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTCACCCGCTGGCCCTGCCGGTGACCGGTG.ATCAGCGGTCGCGccccccc                                                     >  2:644228/1‑100 (MQ=37)
                                                cGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCcgcg                                                   <  2:3143124/101‑1 (MQ=255)
                                                cGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCcgcg                                                   >  2:4319255/1‑101 (MQ=255)
                                                 gggTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCgg                                                  <  1:1260656/101‑1 (MQ=255)
                                                   gTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCgggg                                                >  1:3502965/1‑101 (MQ=255)
                                                      aCGAAACGGCAGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCg                                             <  1:4498723/101‑1 (MQ=255)
                                                        gAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCggg                                           >  1:2365215/1‑99 (MQ=255)
                                                        gAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTg                                           >  2:30664/1‑101 (MQ=255)
                                                         tcaacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.GCTGGCGCGCGCCGCGCCG..GATCTTTGTCTGCGCGGTCGCTCCCCCCGCGGGGGCGTGGAt                                        >  2:2494299/9‑101 (MQ=2)
                                                         aaaCGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.CTCAGCGGGCGCTCCCCCCCCGGGGGCGTgg                                          >  2:4519730/1‑101 (MQ=255)
                                                          caacactgTCGCACCCCCCGCGGGGGCGTGGATCGAAAC.GCTGGCGCGCGCCGCGCCG..GATCTTTGTCTGCGCGGTCGCTCCCCCCGCGGGGGCGTGGATc                                       >  1:2528256/8‑101 (MQ=2)
                                                          aaCGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGa                                         >  1:4494640/1‑101 (MQ=255)
                                                          aaCGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGa                                         <  1:1814703/101‑1 (MQ=255)
                                                           aacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.GCTGGCGCGCGCCGCGCCG..GATCTTTGTCTGCGCGGTCGCTCCCCCCGCGGGGGCGTGGATCg                                      <  1:154476/95‑1 (MQ=2)
                                                           aacacggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.GCTGGCGCGCGCCGCGCCG..GATCTTTGTCTGCGCGGTCGCTCCCCCCGCGGGGGCGTGGATCg                                      <  2:1727026/95‑1 (MQ=2)
                                                            cGGCAGTCCCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATc                                       <  2:3648930/101‑1 (MQ=255)
                                                            acactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.GCTGGCGCGCGCCGCGCCG..GATCTTTGTCTGCGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                     >  1:165982/6‑101 (MQ=2)
                                                             ggCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCg                                      >  1:413753/1‑101 (MQ=255)
                                                             cactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.GCTGGCGCGCGCCGCGCCG..GATCTTTGTCTGCGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                    >  1:4390574/5‑101 (MQ=2)
                                                             cactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.GCTGGCGCGCGCCGCGCCG..GATCTTTGTCTGCGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                    >  1:4216720/5‑101 (MQ=2)
                                                             cactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.GCTGGCGCGCGCCGCGCCG..GATCTTTGTCTGCGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                    <  1:218179/97‑1 (MQ=2)
                                                              cctgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.GCTGGCGCGCGCCGCGCCG..GATCTTTGTCTGCGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                   <  1:1424812/98‑1 (MQ=2)
                                                                tgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.GCTGGCGCGCGCCGCGCCG..GATCTTTGTCTGCGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                 >  1:3095857/2‑100 (MQ=2)
                                                                aGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                   >  2:3251534/1‑101 (MQ=255)
                                                                aGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                   >  1:2888290/1‑101 (MQ=255)
                                                                 gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                  <  2:2349928/101‑1 (MQ=255)
                                                                 gTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.GCTGGCGCGCGCCGCGCCG..GATCTTTGTCTGCGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActc                                >  1:3860529/1‑99 (MQ=2)
                                                                   cGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAAAt                                >  1:60779/1‑101 (MQ=255)
                                                                      tCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgc                             <  2:1389185/101‑1 (MQ=255)
                                                                       ccccccGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgcc                            <  2:437496/101‑1 (MQ=255)
                                                                       ccccccGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgcc                            <  2:3002593/101‑1 (MQ=255)
                                                                       ccccccGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgcc                            <  1:1019681/101‑1 (MQ=255)
                                                                              cgGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTgg                     >  2:2610689/1‑101 (MQ=255)
                                                                              cgGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTgg                     >  2:2237069/1‑101 (MQ=255)
                                                                              cgGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTgg                     >  1:1191417/1‑101 (MQ=255)
                                                                                ggggCGGGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCg                   <  2:3891237/101‑1 (MQ=255)
                                                                                   gCGTGGATCGAAACTGCGCCCGCTTGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTc                <  2:4585447/101‑1 (MQ=255)
                                                                                   gCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTc                <  1:3508257/101‑1 (MQ=255)
                                                                                    cGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCg               <  2:4435355/101‑1 (MQ=255)
                                                                                    cGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCg               >  1:2453810/1‑101 (MQ=255)
                                                                                     gtcgATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGa              <  2:3931072/98‑1 (MQ=255)
                                                                                     gTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGa              >  1:994749/1‑101 (MQ=255)
                                                                                     gTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGa              >  1:2759213/1‑101 (MQ=255)
                                                                                     gTGGAGCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGTTCTGTCCCGCCGCGGGGGCGTGGATCGACATATCGCCGCTTTGGCGGTCGa              >  2:806072/1‑101 (MQ=255)
                                                                                      tGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.GTTAACGGTCGGTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGGCggc             >  2:1540596/1‑99 (MQ=37)
                                                                                      tGGATCGAAACTGCACCCGCTGGCCCTGCCGGTAATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGAc             <  1:3981298/101‑1 (MQ=255)
                                                                                      tGGATCGAAACTGAACCCGCTGGACCTGCCGGTGATCGGTA.ATCAGCGGTCGCTCCCACCGCGGGGGCGTGGATTGAAACATCGCCGCTTTTGCGGTCgga             >  1:1513968/1‑99 (MQ=255)
                                                                                       ggATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACa            >  2:3479144/1‑101 (MQ=255)
                                                                                       ggATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACa            <  1:2348909/101‑1 (MQ=255)
                                                                                          tCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGc         <  2:2984023/101‑1 (MQ=255)
                                                                                              aaCTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGt     <  2:1450177/101‑1 (MQ=255)
                                                                                               aCTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTa    >  2:3229170/1‑101 (MQ=255)
                                                                                                 tGCCCCCGCTGGCCCTGGGGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAAc  <  1:4646387/101‑1 (MQ=255)
                                                                                                 tGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAAc  <  1:4413331/101‑1 (MQ=255)
                                                                                                 tGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGCCGACAGGATGGTAAc  >  2:167793/1‑101 (MQ=255)
                                                                                                    |                                                                                                  
GCGACAAGCACCTTCGCACCACCGGAGCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAAC  >  NC_014034/1395300‑1395497

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG