Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,411 C→G 8.3% intergenic (+223/‑708) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,4110C→G 8.3% 38.7 ‑0.4 ‑0.0241intergenic (+223/‑708)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (12/8):  ref base (119/102):  total (131/110)
Fisher's exact test strand distribution p-value = 6.46e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.81e-01

Only 100 of 267 total aligned reads displayed.

CTTCGCACCACCGGAGCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCC  >  NC_014034/1395311‑1395511
                                                                                                    |                                                                                                     
cTTCGCACCACCGGAGCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGccc                                                                                                       >  2:3507999/1‑101 (MQ=255)
 ttCGCACCACCGGAGCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCt                                                                                                      <  2:2413992/101‑1 (MQ=255)
 ttCGCACCACCGGAGCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCt                                                                                                      <  2:1906187/101‑1 (MQ=255)
  tCGCACCACCGGAGCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTg                                                                                                     <  2:1314627/101‑1 (MQ=255)
    gCACCACCGGAGCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTTcc                                                                                                   >  2:2902114/1‑101 (MQ=255)
    gCACCACCGGAGCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGcc                                                                                                   >  1:3507817/1‑101 (MQ=255)
    gCACCACCGGAGCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGAACCCGCTGGCCCTGcc                                                                                                   >  2:3981013/1‑101 (MQ=255)
     caccacCGGAGCAATTTACACCGTCTGGTCAGCTGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCcg                                                                                                  <  1:3999730/101‑1 (MQ=255)
       ccaccGGAGCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCcggt                                                                                                <  2:183921/101‑1 (MQ=255)
         accGGAGCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCcggtga                                                                                              >  2:1696756/1‑101 (MQ=255)
             gAGCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCcggtgatcgg                                                                                          >  1:2350769/1‑101 (MQ=255)
              aGCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCcggtgatcggt                                                                                         >  1:2986407/1‑101 (MQ=255)
                cAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCcggtgatcggtg.a                                                                                      >  1:531958/1‑101 (MQ=255)
                cAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCcggcgatcggtg.a                                                                                      >  1:3469367/1‑101 (MQ=255)
                  aTTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCggtgatcggtg.atc                                                                                    >  1:3878072/1‑101 (MQ=255)
                   tttACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCa                                                                                   <  1:1468023/101‑1 (MQ=255)
                   tttACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGGGATCGGTG.ATCa                                                                                   >  2:4031935/1‑101 (MQ=255)
                    ttACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAg                                                                                  <  1:2484104/101‑1 (MQ=255)
                    ttACACCGGCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAg                                                                                  <  2:20289/101‑1 (MQ=255)
                        cccGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGt                                                                              <  1:1106706/100‑1 (MQ=255)
                        acCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGt                                                                              >  2:3220691/1‑101 (MQ=255)
                           gTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGc                                                                           >  2:2300353/1‑101 (MQ=255)
                           gTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGc                                                                           <  1:1874865/101‑1 (MQ=255)
                            tCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCt                                                                          >  2:4224089/1‑101 (MQ=255)
                            tCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCt                                                                          >  1:182349/1‑101 (MQ=255)
                               ggTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTccc                                                                       >  2:3968839/1‑101 (MQ=255)
                               ggTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTccc                                                                       >  1:1225161/1‑101 (MQ=255)
                                gTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGGG.ATCAGCGGTCGCTcccc                                                                      >  2:352498/1‑101 (MQ=255)
                                 tCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTccccc                                                                     <  1:2381168/101‑1 (MQ=255)
                                 tCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTccccc                                                                     >  1:3200532/1‑101 (MQ=255)
                                   aGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCcg                                                                   <  1:3292896/101‑1 (MQ=255)
                                   aGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTCACCCGCTGGCCCTGCCGGTGACCGGTG.ATCAGCGGTCGCGccccccc                                                                   >  2:644228/1‑100 (MQ=37)
                                    gCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCcgc                                                                  <  1:3417436/101‑1 (MQ=255)
                                     cGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCcgcg                                                                 <  2:3143124/101‑1 (MQ=255)
                                        gTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCgggg                                                              <  1:2338731/101‑1 (MQ=255)
                                         ttACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCggggg                                                             <  2:2978797/101‑1 (MQ=255)
                                         ttACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCggggg                                                             >  1:4588213/1‑101 (MQ=255)
                                          tACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGc                                                            <  2:1193667/101‑1 (MQ=255)
                                            cGAAACGGCAGCCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGGCCTGCCGGCGCTCGGTG.ATCAGCGGTCTCTCTTCCCGCGGGGGCGt                                                          >  2:3080792/1‑101 (MQ=37)
                                             gAAAGGGCGGTCGCTCCCCCCGCGGGGGCGTGGTGCGAAACTGCCCCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTg                                                         <  1:3978501/101‑1 (MQ=38)
                                             gAAACGGCAGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTg                                                         <  2:3244209/101‑1 (MQ=255)
                                              tcaacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.GCTGGCGCGCGCCGCGCCG..GATCTTTGTCTGCGCGGTCGCTCCCCCCGCGGGGGCGTGGAt                                                      >  1:4524058/9‑101 (MQ=2)
                                              tcaacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.GCTGGCGCGCGCCGCGCCG..GATCTTTGTCTGCGCGGTCGCTCCCCCCGCGGGGGCGTGGAt                                                      >  1:3692723/9‑101 (MQ=2)
                                              aaaCGGCAGTCGCTCCCCCCGCGGGGGCGTTGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTgg                                                        <  2:3443017/101‑1 (MQ=255)
                                              aaaCGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTgg                                                        <  1:4436026/101‑1 (MQ=255)
                                               aaCGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGa                                                       >  1:4494640/1‑101 (MQ=255)
                                               aaCGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGa                                                       <  1:1814703/101‑1 (MQ=255)
                                                aacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.GCTGGCGCGCGCCGCGCCG..GATCTTTGTCTGCGCGGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                    <  1:4187166/95‑1 (MQ=2)
                                                aacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.GCTGGCGCGCGCCGCGCCG..GATCTTTGTCTGCGCGGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                    <  1:154476/95‑1 (MQ=2)
                                                 cGGCAGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATc                                                     <  2:3281574/101‑1 (MQ=255)
                                                 cGGCAGTCCCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATc                                                     <  2:3648930/101‑1 (MQ=255)
                                                 acactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.GCTGGCGCGCGCCGCGCCG..GATCTTTGTCTGCGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                   >  1:3940869/6‑101 (MQ=2)
                                                  cactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.GCTGGCGCGCGCCGCGCCG..GATCTTTGTCTGCGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                  <  2:2197567/97‑1 (MQ=2)
                                                   gCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCCCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                   <  1:749198/101‑1 (MQ=255)
                                                   gCAGTCCCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                   <  2:4574396/101‑1 (MQ=255)
                                                    cAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                  <  2:3004297/101‑1 (MQ=255)
                                                    cAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                  <  2:4471129/101‑1 (MQ=255)
                                                    cAGACGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGATGGACCTGCCGGTGATCGGTG.ATCAGCGGTCGCACCCCCCGCGGGGGCGTGGATCGaa                                                  >  2:438207/1‑101 (MQ=38)
                                                     tgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.GCTGGCGCGCGCCGCGCCG..GATCTTTGTCTGCGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                               >  2:1752721/2‑100 (MQ=2)
                                                     aGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                 >  2:2555926/1‑101 (MQ=255)
                                                     aGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                 <  1:4347029/101‑1 (MQ=255)
                                                     aGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                 >  1:2888290/1‑101 (MQ=255)
                                                     aGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGGGGATCGaaa                                                 >  1:593584/1‑101 (MQ=255)
                                                     aGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                 <  1:4633246/101‑1 (MQ=255)
                                                       tCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                               <  2:182325/101‑1 (MQ=255)
                                                       tCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                               <  1:1755816/101‑1 (MQ=255)
                                                         gCTCCCCCCGCGGGGGCGTGGAGCGACGCTGCGCCTGGTGGCCCTGCCGGTGATCGGGG.ATCAGCGGTCCCCCCCCCCGCGGGGGCGTGGATCGAAACATc                                             <  2:1604010/101‑1 (MQ=25)
                                                          cTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcg                                            >  1:3751117/1‑101 (MQ=255)
                                                           tCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgc                                           <  2:1389185/101‑1 (MQ=255)
                                                           tCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgc                                           >  2:234818/1‑101 (MQ=255)
                                                           tCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGCCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgc                                           >  2:4364462/1‑101 (MQ=255)
                                                            ccccccGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgcc                                          <  1:1019681/101‑1 (MQ=255)
                                                               cccGCGGGGGCGTGGATCGAAACTGCCCCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCt                                       <  1:2669007/101‑1 (MQ=255)
                                                                    gggggCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGc                                  >  1:4284900/1‑101 (MQ=255)
                                                                    gggggCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGc                                  <  1:359693/101‑1 (MQ=255)
                                                                     ggggCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCg                                 >  1:2100205/1‑101 (MQ=255)
                                                                     ggggCGGGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCg                                 <  2:3891237/101‑1 (MQ=255)
                                                                      gggCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCgg                                <  2:1764964/101‑1 (MQ=255)
                                                                       ggCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGt                               <  1:2044196/101‑1 (MQ=255)
                                                                        gCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTc                              >  1:2982898/1‑101 (MQ=255)
                                                                          gtcgATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGa                            <  2:3931072/98‑1 (MQ=255)
                                                                          gTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGa                            >  1:994749/1‑101 (MQ=255)
                                                                          gTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGa                            >  1:2759213/1‑101 (MQ=255)
                                                                          gTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGa                            >  1:41322/1‑101 (MQ=255)
                                                                          gTGGATCGAAACCGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGa                            <  2:2742043/101‑1 (MQ=255)
                                                                          gTGGAGCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGTTCTGTCCCGCCGCGGGGGCGTGGATCGACATATCGCCGCTTTGGCGGTCGa                            >  2:806072/1‑101 (MQ=255)
                                                                           tGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGGGTGGATCGAAACATCGCCGCTTTGGCGGTAGAc                           >  1:1643886/1‑101 (MQ=255)
                                                                           tGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTTGGGGTCggc                           >  1:4392100/1‑99 (MQ=255)
                                                                           tGGATCGAAACTGCACCCGCTGGCCCTGCCGGCGATCGGTG.ATCAGCGGTCCCTCCCCCCGCGGGGGGGTGGATCGAAACATCGCCGCTTTGGCGGGCGAc                           >  1:623051/1‑101 (MQ=255)
                                                                             gATCGAAACTGCCCCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAg                         <  1:3081036/101‑1 (MQ=255)
                                                                             gATCGAAACTGCCCCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAg                         <  1:4319545/101‑1 (MQ=255)
                                                                             gATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAg                         >  2:2815872/1‑101 (MQ=255)
                                                                               tCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGc                       <  2:2984023/101‑1 (MQ=255)
                                                                                cGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCt                      >  1:875021/1‑101 (MQ=255)
                                                                                cGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCt                      >  1:1286704/1‑101 (MQ=255)
                                                                                   aaCTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGt                   <  2:1450177/101‑1 (MQ=255)
                                                                                   aaCTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGt                   <  2:4587914/101‑1 (MQ=255)
                                                                                    aCTGCCCCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTa                  <  1:1164256/101‑1 (MQ=255)
                                                                                               tGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTc       >  1:924648/1‑101 (MQ=255)
                                                                                               tGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTc       >  2:20757/1‑101 (MQ=255)
                                                                                                    |                                                                                                     
CTTCGCACCACCGGAGCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCC  >  NC_014034/1395311‑1395511

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG