Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,467 T→A 16.1% intergenic (+279/‑652) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,4670T→A16.1% 116.9 ‑0.5 ‑0.0254intergenic (+279/‑652)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (23/18):  ref base (109/104):  total (132/122)
Fisher's exact test strand distribution p-value = 6.11e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.08e-01

Only 100 of 276 total aligned reads displayed.

CGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCTCG  >  NC_014034/1395367‑1395567
                                                                                                    |                                                                                                    
cGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAAAt                                                                                                      >  1:60779/1‑101 (MQ=255)
   tCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgc                                                                                                   >  2:234818/1‑101 (MQ=255)
    ccccccGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgcc                                                                                                  <  1:1019681/101‑1 (MQ=255)
    ccccccGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgcc                                                                                                  <  1:3443503/101‑1 (MQ=255)
    ccccccGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgcc                                                                                                  <  2:437496/101‑1 (MQ=255)
          gcgGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTg                                                                                            <  1:4320703/101‑1 (MQ=255)
           cgGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTgg                                                                                           >  2:2610689/1‑101 (MQ=255)
            gggggCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGc                                                                                          <  1:359693/101‑1 (MQ=255)
                 cGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCg                                                                                     >  1:1789802/1‑101 (MQ=255)
                 cGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCg                                                                                     <  2:4435355/101‑1 (MQ=255)
                  gTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGa                                                                                    >  1:2759213/1‑101 (MQ=255)
                  gTGGAGCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGTTCTGTCCCGCCGCGGGGGCGTGGATCGACATATCGCCGCTTTGGCGGTCGa                                                                                    >  2:806072/1‑101 (MQ=255)
                   tGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGGGTGGATCGAAACATCGCCGCTTTGGCGGTAGAc                                                                                   >  1:1643886/1‑101 (MQ=255)
                   tGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTTGGGGTCggc                                                                                   >  1:4392100/1‑99 (MQ=255)
                   tGGATCGAAACTGCACCCGCTGGCCCTGCCGGTAATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGAc                                                                                   <  1:3981298/101‑1 (MQ=255)
                    ggATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACa                                                                                  >  1:3355550/1‑101 (MQ=255)
                    ggATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACa                                                                                  >  2:3479144/1‑101 (MQ=255)
                     gATCGAAACTGCCCCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAg                                                                                 <  1:4319545/101‑1 (MQ=255)
                     gATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAg                                                                                 <  2:531910/101‑1 (MQ=255)
                       tCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGc                                                                               <  2:2984023/101‑1 (MQ=255)
                        cGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCt                                                                              >  1:1286704/1‑101 (MQ=255)
                        cGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCt                                                                              >  1:875021/1‑101 (MQ=255)
                           aaCTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGt                                                                           <  2:4587914/101‑1 (MQ=255)
                           aaCTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGt                                                                           <  2:1450177/101‑1 (MQ=255)
                            aCTGCCCCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTa                                                                          <  1:1164256/101‑1 (MQ=255)
                            aCTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTa                                                                          <  2:3998365/101‑1 (MQ=255)
                            aCTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTa                                                                          >  2:3229170/1‑101 (MQ=255)
                              tGCCCCCGCTGGCCCTGGGGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAAc                                                                        <  1:4646387/101‑1 (MQ=255)
                              tGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAAc                                                                        >  2:3542194/1‑101 (MQ=255)
                                cACCCGCTGGCCCTGCCGGTGATCGGGGATCAGCGGTCGCTCCCCCCGCGGGGGCGGGGATCGAAAGATCGCCGCTTTGGCGGCTTCCAGGCTggataagc                                                                      >  2:2461677/1‑95 (MQ=25)
                                 ccccGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACgtg                                                                     <  1:2342844/100‑1 (MQ=255)
                                  cccGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACgtgt                                                                    <  1:352530/101‑1 (MQ=255)
                                  cccGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACgtgt                                                                    <  2:2888007/101‑1 (MQ=255)
                                   ccGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTc                                                                   <  2:60773/101‑1 (MQ=255)
                                   ccGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTc                                                                   >  1:4596619/1‑101 (MQ=255)
                                     gCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGc                                                                 <  2:3362020/101‑1 (MQ=255)
                                      cgggCCCTGCTGGTGATCGGTGATCAGCGGTCTCAACGCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCt                                                                <  2:1333544/99‑1 (MQ=38)
                                      cTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCt                                                                <  1:4372012/101‑1 (MQ=255)
                                       tGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTc                                                               >  2:20757/1‑101 (MQ=255)
                                       tGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTc                                                               >  1:924648/1‑101 (MQ=255)
                                           ccTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTccccc                                                           <  1:2061497/101‑1 (MQ=255)
                                            cTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTcccccc                                                          <  2:939740/101‑1 (MQ=255)
                                             tGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCcg                                                         >  2:2494859/1‑101 (MQ=255)
                                              gCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCcgc                                                        >  1:2358714/1‑101 (MQ=255)
                                                cggtgatcggtgatCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCgg                                                      >  2:1593180/1‑101 (MQ=255)
                                                cggtgatcggtgatCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCgg                                                      <  2:2759015/101‑1 (MQ=255)
                                                  gtgatcggtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCgggg                                                    <  2:622990/101‑1 (MQ=255)
                                                  gtgatcggtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCgggg                                                    >  1:2676108/1‑101 (MQ=255)
                                                  gtgatcggtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCgggg                                                    >  1:4089704/1‑101 (MQ=255)
                                                  gtgatcggtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCgggg                                                    >  2:508400/1‑101 (MQ=255)
                                                   tgatcggtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGNCGCTCCCCCCGCggggg                                                   >  2:212557/1‑101 (MQ=255)
                                                    gatcggtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGc                                                  >  1:2852068/1‑101 (MQ=255)
                                                     atcggtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCg                                                 <  1:3220934/101‑1 (MQ=255)
                                                      tcggtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGt                                                <  2:4051398/101‑1 (MQ=255)
                                                        cgctggacgtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGa                                             <  1:1485202/91‑1 (MQ=2)
                                                         gtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGa                                             <  1:391909/101‑1 (MQ=255)
                                                         gtgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGa                                             <  1:482771/101‑1 (MQ=255)
                                                         gctggacgtggTCGATCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCNCCCGCGGGGGCGTGGAt                                            >  2:425545/10‑101 (MQ=2)
                                                          tgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGAt                                            <  2:3900524/101‑1 (MQ=255)
                                                          tgatcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGGgggt                                            >  2:1759181/1‑99 (MQ=255)
                                                            atcAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCg                                          <  2:3502707/101‑1 (MQ=255)
                                                               cgtgtgcaccccccccGCGGGGGCGTGGATCGAAACTACGCCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCGCCCCCCGCGGGGGCGTGGATCGAAAc                                      <  2:2539883/91‑1 (MQ=2)
                                                                gtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                     >  1:4053380/3‑101 (MQ=2)
                                                                gCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                      <  1:2986182/101‑1 (MQ=255)
                                                                 tggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACag                                    >  1:4272097/2‑100 (MQ=2)
                                                                  ggTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                    <  1:2210832/101‑1 (MQ=255)
                                                                    tCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgctt                                 >  1:3671143/1‑100 (MQ=2)
                                                                      gCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCg                                >  1:655815/1‑101 (MQ=255)
                                                                      gCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCg                                >  1:2668713/1‑101 (MQ=255)
                                                                       cTCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgctttca                              >  1:1956997/1‑97 (MQ=2)
                                                                       cTCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgctttca                              <  1:554453/101‑5 (MQ=2)
                                                                       cTCCCCCCGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgctttca                              <  2:2704355/101‑5 (MQ=2)
                                                                       cTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCgg                               <  1:963569/101‑1 (MQ=255)
                                                                       cTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCgg                               <  1:3479402/101‑1 (MQ=255)
                                                                        tCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGt                              >  1:1401946/1‑101 (MQ=255)
                                                                         ccccccGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgctttcaat                            >  1:1844075/1‑95 (MQ=0)
                                                                         ccccccGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgctttcaat                            >  2:1943675/1‑95 (MQ=0)
                                                                          cccccGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgctttcaatg                           >  1:4473903/1‑94 (MQ=2)
                                                                          cccccGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgctttcaatg                           >  2:2795852/1‑94 (MQ=2)
                                                                          cccccGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGc                            <  2:4573908/101‑1 (MQ=255)
                                                                           ccccGCGGGGGCGTGGATCGAAACTACACCGGGGCTCTGGTC.ACGGTTTGGGACGGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAgctttcaatgg                          <  1:4510379/101‑9 (MQ=2)
                                                                            cccGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCg                          <  2:1716733/101‑1 (MQ=255)
                                                                             ccGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGt                         >  2:1986835/1‑101 (MQ=255)
                                                                              cgcgGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTc                        <  2:777702/101‑1 (MQ=255)
                                                                              cgcgGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTc                        <  1:2461843/101‑1 (MQ=255)
                                                                               gcgGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCg                       <  2:3650410/101‑1 (MQ=255)
                                                                                 gggggCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCt                     <  1:2495025/101‑1 (MQ=255)
                                                                                  ggggCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTc                    >  1:1514033/1‑101 (MQ=255)
                                                                                    ggCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGt                  <  2:1782558/101‑1 (MQ=255)
                                                                                      cGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGGGCCGTCGCTCGTcc                >  2:1423074/1‑101 (MQ=255)
                                                                                      cGTCGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTcc                >  2:311519/1‑101 (MQ=255)
                                                                                       gTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCTGTGCCGCCGCTCGTCCa               <  2:3877787/101‑1 (MQ=255)
                                                                                       gTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCa               <  1:2966163/101‑1 (MQ=255)
                                                                                       gTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCa               >  2:1519881/1‑101 (MQ=255)
                                                                                             cGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGc         >  1:3863634/1‑101 (MQ=255)
                                                                                             cGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGc         >  1:4235/1‑101 (MQ=255)
                                                                                                aaCATCGCCGCTTTGGCGGTCGACAGGCTGGGAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTg      >  1:4133367/1‑101 (MQ=255)
                                                                                                   aTCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCtc   <  2:3750842/101‑1 (MQ=255)
                                                                                                   aTCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCtc   >  2:1108139/1‑101 (MQ=255)
                                                                                                   aTCGCCGCTTTGGCGGTAGACAGGCTGGTAACGTGTCCCGCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCtc   <  2:3417925/101‑1 (MQ=255)
                                                                                                    |                                                                                                    
CGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTGATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAACGTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCTCGGTGCCGTCGCTCGTCCAGCCGGCCTGCTCG  >  NC_014034/1395367‑1395567

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG